Hemax530AL Fluidic Diagram VV

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3 Hydraulics System

3.3 Hydraulics Diagram for the Overall Unit


3-way valve 2-way valve
2:COM
Hydraulics Diagram
2:IN
1:NC 1:OUT
3:NO

sheath flow
pressure Flow
sensor chamber

Diluent DIFF I DIFF II HGB

Air Filter
Diluent Lyse
syringe syringe
(10ml) (2.5ml) One-way
valve
Sheath
positive-pressure
Flow Syringe
Sampling sensor casing pipe
(10ml) Sample flow
syringe
(250ul) syringe Air Filter
(100ul)
casing pipe strapping

casing
pipe positive-pressure
RBC chamber
WBC
liquid pump waste

swab

liquid pump
Isolation
In-line ch amber negative-
sample
filter In-line pressure
probe In-line
Isolation filter chamber
chamber filter
Note
LV-Liquid valve PP-pump
In-line filter
negative-pres sure T-pipe J-connector
sensor

3.4 Time Sequence Description

3.4.1 DIFF Measurement Channel

3.4.1.1 and a of Their


Measurement principles: Flow cytometry and scattering emission of the semiconductor laser
Measurement parameters: Mon#, Mon%, Lym#, Lym%, Neu#, Neu%, Eos#, Eos%, Bas#, Bas%
Graph information: one WBC 3D scattergram, three WBC 2D scattergrams
Reagents for use
DIFF I & DIFF II lyse: Lyses the RBCs and specifically treats different types of WBCs.
Diluent: Provides sheath flow fluid, used for cleaning.
Function description
The sample is mixed well for a full reaction with DIFF I lyse and DIFF II lyse in the DIFF bath. Then
the sample is placed between the flow chamber inlet and the sample injection tube; meanwhile, the
sheath flow is activated and kept steady so that it can hold the sample in motion until it reaches the
measurement section of the flow chamber. The sample injection tube, at a constant speed and within

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