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Abstract
Background: Despite efforts made to reduce the spread of the human immune-deficiency virus (HIV), its testing
coverage remains low in low and middle-income countries (LMIC). Besides, information on factors associated with
HIV counseling and testing among reproductive-age women is not sufficiently available. Therefore, this study was
aimed to determine the pooled prevalence and factors associated with HIV testing among reproductive-age
women in eastern Africa.
Methods: Secondary data analysis was conducted based on the Demographic and Health Surveys (DHS) data
conducted in East African countries. We pooled the most recent DHS surveys done in 11 East African countries. A
total weighted sample of 183,411 reproductive-age women was included for this study. Both bivariable and
multivariable multilevel logistic regression models were fitted. Variables with a p-value ≤0.2 in the bivariable analysis
were selected for multivariable analysis. Finally, in the multivariable analysis, variables with a p-value ≤0.05 were
considered as significant factors affecting HIV testing.
Results: The pooled prevalence of HIV testing in eastern Africa was 66.92% (95%CI: 66.70, 67.13%). In the
multivariable multilevel analysis factors such as the age of respondent, marital status, educational level, HIV
knowledge, HIV stigma indicator, risky sexual behavior and women who visit a health facility were positively
associated with HIV testing coverage among reproductive-age women. While women from rich and richest
households, having multiple sexual partners, being from rural dwellers, late initiation of sex and higher community
illiteracy level had a lower chance of being tested for HIV.
* Correspondence: [email protected]
1
Department of Human Anatomy, College of Medicine and Health Science,
School of Medicine, University of Gondar, Gondar, Ethiopia
Full list of author information is available at the end of the article
© The Author(s). 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License,
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The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the
data made available in this article, unless otherwise stated in a credit line to the data.
Worku et al. BMC Public Health (2021) 21:1262 Page 2 of 9
Conclusion: The pooled prevalence of HIV testing in eastern Africa was higher than most previous studies. Age of
respondent, residence, wealth index, marital status, educational level, HIV knowledge, stigma indicator, risky sexual
behavior, women who visit a health facility, multiple sexual partnerships, early initiation of sex and community
illiteracy level were significantly associated with HIV testing. There should be an integrated strategic plan to give
education about methods of HIV transmission and the implication of HIV testing and counseling. So all the
stakeholders should have an integrated approach by giving special attention to the factors that hinder HIV testing
to increase awareness regarding the benefit of HIV testing and counseling to control the spread of HIV/AIDS.
Keywords: Multi-level analysis, HIV/AIDS, HIV testing, Eastern Africa
Table 2 Sociodemographic characteristics of the respondents in Table 2 Sociodemographic characteristics of the respondents in
eastern Africa (N = 183, 411) eastern Africa (N = 183, 411) (Continued)
Variables Frequency (%) Variables Frequency (%)
Age (years) At 20 and after years 74,878 (40.83%)
15–19 39,510 (21.54%) Visit health facility
20–24 34,150 (18.62%) No 72,075 (43.18%)
25–29 31,766 (17.32%) Yes 94,857 (56.82%)
30–34 26,992 (14.72%) Community illiteracy level
35–39 21,886 (11.93%) High 90,641 (49.42%)
40–44 16,406 (8.94%) Low 92,770 (50.58%)
45–49 12,701 (6.92%)
Highest education level
Operating Curve (ROC). These are plotted based on the
No education 33,035 (18.01%)
probability of sensitivity and 1 – specificity. Accordingly,
Primary education 89,229 (48.65%) the AUC of the final model was 84.5% and indicated that
Secondary education 51,294 (27.97%) the model’s ability to predict HIV testing was good
Higher education 9,840 (5.37%) (Fig. 2).
Wealth index
Factors associated with HIV testing
Poorest 32,495 (17.72%)
For assessing factors associated with HIV testing, we
Poorer 33,755 (18.40%)
consider the final model since it had the lowest deviance.
Middle 34,934 (19.05%) In the multivariable multilevel analysis factors such as
Richer 37,225 (20.30%) the age of respondent, marital status, educational level,
Richest 45,001 (24.54%) HIV knowledge, HIV stigma indicator, risky sexual be-
Risky sexual behavior havior and women who visit a health facility were posi-
tively associated with HIV testing coverage among
No risk 129,912 (81.70%)
reproductive-age women. While women from rich and
Some risk 20,169 (12.68%)
richest households, having multiple sexual partners, be-
High risk 8,931 (5.62%) ing from rural dwellers, late initiation of sex and higher
HIV knowledge community illiteracy level had a lower chance of being
Low knowledge 17,049 (10.03%) tested for HIV. Women aged 20 years and above had
High knowledge 71,694 (42.16%) higher odds of being tested for HIV compared with
women of 15–19 years old. The odds of testing for HIV
Comprehensive knowledge 81,301 (47.81%)
was 2.67 (AOR = 2.67: 95%CI; 2.60, 2.74) times higher
Marital status
for women visiting health centers in the last 12 months
Unmaried 88,582 (48.30%) compared with their counterparts. Regarding educational
Maried 94,829 (51.70%) level, the odds of testing for HIV was 2.30 (AOR = 2.30:
Number of sexual partners 95%CI; 2.22, 2.38), 2.39 (AOR = 2.39: 95%CI; 2.29, 2.50)
One 72,463 (39.51%) and 3.13 (AOR = 3.13: 95%CI; 2.89, 3.39) times higher
for women with primary, secondary and higher educa-
More than one 110,948 (60.49%)
tional level, respectively, compared to women with no
Stigma indicator
formal education. Being from rich (AOR = 0.93: 95%CI;
No stigma 5,961 (7.15%) 0.89, 0.97) and richest (AOR = 0.80: 95%CI; 0.76, 0.84)
Low stigma 35,612 (42.71%) households had lower odds of being tested for HIV. Be-
Moderate stigma 34,231 (41.06%) ing married had 1.32 (AOR = 1.32: 95%CI; 1.28, 1.36)
High stigma 7,569 (9.08%) times more likely to be tested for HIV compared with
their counterpart. Individuals who initiate sex after 20
Residence
years of age had 33% (AOR = 0.67: 95%CI; 0.65, 0.69)
Urban 51,426 (28.04%)
lower odds of being tested for HIV compared to those
Rural 131,985 (71.96%) who initiate sex early. Regarding stigma, women with
Age at sex higher, moderate and low stigma scores had 1.56
Before 20 years 108,533 (59.17%) (AOR = 1.56: 95%CI; 1.46, 1.66), 2.24 (AOR = 2.24:
95%CI; 2.16, 2.32), and 2.27 (AOR = 2.27: 95%CI; 2.19,
Worku et al. BMC Public Health (2021) 21:1262 Page 5 of 9
2.35) times higher odds of being tested for HIV com- from region to region. The regional variations in qual-
pared to those with no stigma. Women with higher ity and access to HIV testing facilities as well as know-
(AOR = 6.44: 95%CI: 6.21, 6.68) and comprehensive ledge related to HIV / AIDS may be the reasons for
knowledge (AOR = 10.7: 95%CI; 10.29, 11.12) about the reported regional variations of HIV testing in east-
HIV/AIDS had higher odds of being tested for HIV ern Africa [9, 14]. The prevalence reported in this
compared with women with low knowledge. Women study was in line with the report in Kenya [12]. The
having high (AOR = 1.78: 95%CI; 1.67, 1.90) and some finding in this study was greater than the study con-
risky sexual behavior (AOR = 1.59: 955CI; 1.53, 1.66) had ducted elsewhere [3, 16, 23] and it was smaller than
higher odds of being tested for HIV compared to women reports from different studies [9, 10]. The observed
with no risky sexual behavior. Women with multiple variations in the prevalence of HIV testing might be
sexual partners had a 36% lower chance of being tested explained by cultural beliefs and lifestyle differences
for HIV (AOR = 0.64: 95%CI; 0.62, 0.66) as compared to across regions [14]. Besides, the discrepancy might be
their counterparts. Women from the rural areas had due to the difference in quality and accessibility of
31% (AOR = 0.69: 95%CI; 0.67, 0.72) lower odds of being HIV testing facilities [9, 14, 24, 25].
tested for HIV compared with their counterparts. Being In this study age of respondent, multiple sexual part-
from communities with higher community illiteracy nerships, marital status, visiting health facility, stigma-
levels had 27% (AOR = 0.73: 95%CI; 0.68, 0.78) lower tized attitude towards HIV/AIDS, HIV knowledge, risky
odds of being tested for HIV compared with women sexual behavior, residence, educational level, wealth sta-
from communities with lower community illiteracy tus, age at first sex, and community illiteracy level were
levels (Table 4). significant factors associated with HIV testing.
The odds of HIV testing was higher among married
Discussion women compared with their counterpart. This finding
The pooled prevalence of HIV testing in east Africa was in agreement with the study done in Ethiopia [22].
was 66.9% (95%CI; 66.70, 67.13%), which varies greatly This might be due to compulsory counseling and
Table 3 Random effect model and model fitness for the assessment of HIV testing among reproductive-age women in eastern
Africa
Parameter Null model Model I Model II Model III
Intraclass correlation coefficient (ICC) 0.10031 0.0692 0.0782476 0.066394
Percentage change in variation (PCV) Ref 0.33 0.39 0.36
Median odds ratio (MOR) 1.77 1.6 1.65 1.58
Model comparison
Log likelihood − 113956.33 −75036.661 −113215.37 −74814.426
Deviance 227912.66 150073.322 226420.47 149628.852
Worku et al. BMC Public Health (2021) 21:1262 Page 6 of 9
testing promotion for couples intending to get mar- friendship, family relations, jobs and housing and health
ried by different organizations including religious care due to stigmatization [2, 18, 22]. However, in the
groups [22]. Women who visit health care facilities present study people with stigmatized attitudes had a
had higher odds of being tested for HIV, which was higher chance of being tested for HIV and this might be
supported by another study [16]. This might be be- explained by the variation in the cultural and socioeco-
cause health professionals initiate people who visited nomic status of the population included in this study.
health facilities for HIV testing [16]. In this study, women with risky sexual behavior had
In this study women from higher socioeconomic status higher odds of being tested for HIV. This is supported by
had lower odds of being tested for HIV, which is con- another study [22]. Individuals with risky sexual behavior
trary to another study [7]. This is justified by being rich live under persistent fear and uncertainty about their ser-
may be associated with a greater awareness of risks and ostatus and are usually suspicious and worried that they
with reduced financial barriers to testing [26]. Women might have infected with HIV. This urges them to develop
with primary and above educational levels had a higher habits of seeking VCT service [17].
chance of being tested for HIV, which is supported by Individuals who initiated sex early had a higher chance
different studies [16, 27]. The reason for this discrepancy of being tested for HIV and this is supported by the
might be as education can improve HIV knowledge as study conducted in Malawi [14]. This might be ex-
well as empowers women to make decisions to visit the plained as early age at first sexual intercourse is associ-
health facility and use health services [28]. ated with a higher risk of acquiring the different sexually
Women who had multiple sexual partners had less transmitted disease and risky sexual behaviors that may
chance of being tested for HIV compared with their lead to a higher risk for HIV infection, which in turn
counterparts. However, the finding of this study was in enforce them to know their HIV status [29].
contrast to another study [16]. This difference might be Women from rural dwellers had lower odds of be-
associated with individuals with a history of multiple ing tested for HIV, which was supported by the study
sexual partnerships who might be fear of having HIV conducted in Ethiopia [16]. This may be justified by
and have no interest to know their status. The study at the better availability and accessibility of HIV testing
hand revealed that women with higher and comprehen- facilities in urban settings compared with rural [30,
sive knowledge about HIV had higher odds of being 31]. Women from communities with higher illiteracy
tested for HIV. This is supported by a study conducted levels had less chance of being tested for HIV. This
in South Africa [13]. Different studies reported people finding was supported by studies conducted in
with higher HIV-associated stigma scores had less Ethiopia and Zambia [10, 16]. This might be associ-
chance of being tested for HIV. This is explained as ated with educational attainment may increase uptake
people could be hesitant to test because the disclosure of testing through increased recognition of the im-
of a positive HIV test result may lead to loss of portance of knowing one’s HIV status [20, 32].
Worku et al. BMC Public Health (2021) 21:1262 Page 7 of 9
Table 4 The bivariable and multivariable multilevel binary logistic regression analysis of factors associated with HIV testing in East
Africa in the final model
Variables Ever tested for HIV COR(95%CI) AOR(95%CI)
Yes No
Respondent age
15–19 15383 24122 1 1
20–24 25379 8738 4.70 (4.55, 4.86) 3.67 (3.53, 3.82)*
25–29 25, 043 6664 6.33 (6.11, 6.55) 5.36 (5.12, 5.61)*
30–34 21074 5882 6.23 (6.01, 6.47) 5.49 (5.23, 5.76)*
35–39 16333 5521 5.20 (5.00, 5.40) 4.77 (4.54, 5.01)*
40–44 11673 4714 4.16 (3.99, 4.33) 4.12 (3.91, 4.34)*
45–49 7860 4827 2.78 (2.66, 2.90) 3.03 (2.87, 3.20)*
Visiting health facility
No 34413 37624 1 1
Yes 74386 20401 4.06 (3.97, 4.15) 2.67 (2.60, 2.74)*
Highest educational level
No education 17747 15253 1 1
Primary education 61029 28085 1.84 (1.79, 1.89) 2.30 (2.22, 2.38)*
Secondary education 35471 15781 1.81 (1.76, 1.87) 2.39 (2.29, 2.50)*
Higher education 8488 1347 4.58 (4.31, 4.87) 3.13 (2.89, 3.39)*
Wealth status
Poorest 20082 12353 1 1
Poorer 21473 12245 1.25 (1.21, 1.29) 0.97 (0.93 1.02)
Middle 22840 12054 1.38 (1.33, 1.43) 0.98 (0.93 1.02)
Rich 25606 11585 1.56 (1.51, 1.61) 0. 93 (0.89, 0.97)*
Richest 32745 12231 1.71 (1.66, 1.76) 0.80 (0.76, 0.84)*
Marital statues
Married 69948 24745 1.99 (1.95, 2.03) 1.32 (1.28, 1.36)*
Unmarried 52799 35722 1 1
No of sexual partner
One 52493 19907 1 1
More than one 70254 40560 0.55 (0.54, 0.56) 0.64 (0.62 0.66)*
HIV knowledge
Low knowledge 7414 9619 1 1
Higher knowledge 49482 22146 6.9 (6.69, 7.11) 6.44 (6.21, 6.68)*
Comprehensive knowledge 65773 15416 12.6 (12.20, 13.01) 10.7 (10.29 11.12)*
Stigma indicator
Higher stigma 4375 3194 1.05 (1.01, 1.11) 1.56 (1.46, 1.66)*
Moderate stigma 25817 8414 2.30 (2.24, 2.37) 2.24 (2.16, 2.32)*
Low stigma 27813 7799 2.68 (2.61, 2.76) 2.27 (2.19, 2.35)*
No stigma 4905 1056 1 1
Residence
Urban 38964 12404 1 1
Rural 83783 48063 0.66 (0.64, 0.67) 0.69 (0.67, 0.72)*
Risky sexual behavior
Higher risk 7119 1805 2.21 (2.10, 2.33) 1.78 (1.67, 1.90)*
Worku et al. BMC Public Health (2021) 21:1262 Page 8 of 9
Table 4 The bivariable and multivariable multilevel binary logistic regression analysis of factors associated with HIV testing in East
Africa in the final model (Continued)
Variables Ever tested for HIV COR(95%CI) AOR(95%CI)
Yes No
Some risk 15518 4636 1.80 (1.74, 1.87) 1.59 (1.53, 1.66)*
No risk 86043 43784 1 1
Age at sex
Before 20 years 79591 29772 1 1
At 20 and above years 35500 9309 8.66 (8.37, 8.96) 0.67 (0.65, 0.69)*
Community illiteracy level
Low 64381 26126 1 1
High 58366 34341 0.52 (0.48, 0.55) 0.73 (0.68, 0.78)*
*p ≤ 0.05
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