PARKER HANNIFIN CORPORATION

PRECISION FLUIDICS DIVISION

26 CLINTON DRIVE, UNIT 103
HOLLIS, NH 03049 USA
TEL: (603) 595-1500
FAX: (603) 595-8080

PICOSPRITZER® III Manual

Pressure Systems for Ejection of
Picoliter Volumes in Cell Research

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PICOSPRITZER® III

SAFETY

Please read this entire set of instructions before attempting to use this manual for intracellular
or extracellular ejections. This instrument should only be used as specified by the
manufacturer. Use other than as specified may present a safety hazard. It is intended for
research purposes only. Not for use on humans or for diagnostic purposes.

Please do not remove the cover as hazardous voltages may be exposed. Use only the electric
cord supplied with the instrument or one of equal capacity.

For continuing safe operation of the unit periodic inspections of the connections in the pressure
system should be made to ensure they are all still tight, particularly after a period of inactivity or
if the system is moved.

This instrument is intended for use in an indoor environment between 10 and 40 degrees
Celsius (50 to 104 degrees Fahrenheit) with no more than 90% humidity (non-condensing).

EQUIPMENT SPECIFICATIONS
Power Requirements: 100 to 240 Volts AC
50 to 60 HZ
0.8 Amps @ 115 Volts AC
0.6 Amps @ 230 Volts AC

Fuses: 1 Amp, 250 Volts, Slow Blow

5 X 20 millimeters

Maximum Inlet Pressure: 150 PSIG Clean, dry gas

Do not use oxygen, corrosive, or combustible gas

Environmental: Temperature: 10 C (50 F) to 40 C (104 F)

Humidity: 90% non-condensing
Altitude: 0 to 2000 meters
Overvoltage: Category II
Pollution: Degree 2

The instrument is equipped with a universal power supply which will operate on the full
range of voltages between 100 and 240 volts AC.

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PICOSPRITZER® III

TABLE OF CONTENTS

1.0 INTRODUCTION

2.0 SETUP INSTRUCTIONS

3.0 OPERATING SPECIFICATIONS

4.0 CONTOLS AND USE

5.0 BACKGROUND

6.0 REFERENCES

APPENDIX 1 – PART INFORMATION

APPENDIX 2 – DRAWINGS

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1.0 INTRODUCTION

Please read this entire set of instructions before attempting to use this instrument for
intracellular or extracellular ejections. These instructions cover the currently produced
system (P/N 052-0500-900).

The Picospritzer III is a self-contained, rack- mountable system which supplies

repeatable pressure pulses. Volumes dispensed are linear with time and pressure. The
system can be initiated three ways: front panel push button, external stimulator (5 volt),
or remote foot pedal. The Picospritzer III pressure system was designed for rapid and
reproducible ejections of picoliter to nanoliter volumes used in conjunction with
intracellular or extracellular studies while avoiding the inherent desensitization of nerve
cells which accompanies iontophoretic methodology.

Intracellular applications range from femtoliter ejections of RNA into small cells to
nanoliter ejections into oocytes. Extracellular applications range from picoliter
applications of dilute neuroactive substances onto neurons during intracellular recording
to the presentation of chemical stimuli to whole animals.

The system comes complete with high speed valves and the necessary tubing
assemblies (less pipette and holder). It is designed to fit a standard 19" relay rack. The
Picospritzer III comes fully adjusted and ready to use.

2.0 SETUP INSTRUCTIONS

1. Position the control unit in the desired location. Position the remote valve box within
two feet of the experimental site (VELCRO® is supplied for mounting) and within 6 feet
of the control unit.

2. Connect the black ¼ inch pressure tubing to the side port on the front panel regulator
and to a source of clean dry compressed gas (air, nitrogen, CO2, or argon) at a
maximum of 150 PSI (10bar). DO NOT USE OXYGEN OR COMBUSTIBLE GASES.
The fitting on the regulator is a quick- connect type. The end of the tubing should be cut
cleanly and inserted fully into the opening in the fitting. An o-ring seals around the
outside of the tube. To remove the tubing from the fitting you must first vent the pressure
from the line then press the ring on the end of the fitting (around the tubing) toward the
body of the fitting while pulling on the tubing.

3. Use the 6-foot long 1/8-inch tubing assembly to connect the front panel regulator to
the remote valve box. The connection at the regulator is also a quick-connect type while
the remote valve box is threaded (1/4-28 male). There should be a ferrule pressed in to
this end of the tubing.

4. The 3-foot long 1/16-inch tubing assembly is used to connect the remote valve box
to a pipette holder (not supplied). Both ends of this assembly have ¼-28 nuts and
ferrules pre-assembled on it.

PLEASE NOTE: An important detail in arranging the location of the solenoid in relation
to the pipette holder is to maintain a loose coil in the interconnecting small bore (1/16

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 inch) Teflon® tubing to absorb or dampen the pressure pulse, thus avoiding movement
of the pressure pipette. DO NOT stretch the tubing out tight between the valve housing
and the pipette holder.

3.0 OPERATING SPECIFICATIONS

Standard Pressure Range:

10-100 psig, self-bleeding pressure regulator

Pulse Durations:
3 to 999 milliseconds, 1 ms intervals
0.1 to 99.9 seconds, 0.1 sec. intervals
0.1 to 99.9 minutes, 0.1 min. intervals

Pulse Initiation Trigger:

Pushbutton on panel, remote footswitch, or external stimulator (5 volt TTL input BNC
jack).

Time Mark Output:

5-volt TTL signal from front panel BNC jack. References channel one output.

External Inputs:
5-volt TTL input BNC jack per channel.

Remote Valve Box:

24 volt DC high speed enclosed solenoid valve.

4.0 CONTROLS AND USE

The unit is activated when the power switch is in the ON position and the red pilot light is
on. The unit must be connected to a source of clean, dry compressed gas at a pressure
at least as high as that desired for ejections. Clockwise rotation of the knurled knob on
the right of the rack mount panel will increase the pressure (indicated on adjacent
gauge) available for injection purposes. This unit contains a self-bleeding regulator so
that the pressure may be reduced by simply rotating the pressure control knob counter-
clockwise.

The system is rated to operate up to 100 PSI (6.9 bar). One can be assured that all the
connections are gas-tight by rotating the knob on the front panel so that the meter
indicates approximately 80 PSI of pressure and then shutting off the main source of
pressure to the panel. If a gradual reduction of pressure is observed, it indicates that
there is a leak somewhere between the remote valve housing and the main pressure
source. The site of this leak may be found by carefully listening for a hissing sound or by
application of a dilute soap solution (or “snoop”) and watching for the formation of
bubbles at various connections. It is essential to have leak proof connections throughout
the system to assure reproducible results and to avoid unnecessary loss of gases.

Volume ejected is a linear function of both pulse duration and pressure (see references
1, 3). The pulse duration has a greater dynamic range with more accurate and
reproducible settings. Thus, pulse duration will be changed frequently during the course
of the experiment while pressure settings will be changed relatively little on a daily basis.

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 4.1 DURATION SETTING
The pulse duration is indicated by the setting on the three digit thumbwheel switch and
the extended range switch (see Extended Range). It may be initiated by pressing the
adjacent button on the front panel, by remote foot switch, or by an external input signal.
The circuit has a “debounce” control which restricts the action of the push button so that
only one pulse is initiated per button press, even if the button is continuously held down.
It also restricts the interval between repetitive pulses initiated by the push button to
approximately 200 milliseconds. The “PULSE” indicator will light during the course of the
pulse.

4.2 EXTENDED RANGE

The Picospritzer is furnished with extended range timing controlled by a three- position
toggle switch located at the right side of the “Duration” thumbwheel. The top position
(labeled “MSEC”), selects timing in the milliseconds range (2 to 999 milliseconds). In the
center position (labeled “SEC”), the range is from 0.1 to 99.9 seconds (note the
decimal). In the bottom position (labeled “MIN”), the range is from 0.1 to 99.9 minutes.

4.3 INDICATORS
The Picospritzer III contains four LED indicators. “ON” is the power indicator. The
“PULSE” indicator shows that the timer is active; the channel 1 and channel 2 indicators
show when each channel is active.

4.4 INPUT TRIGGER

The internal timer now has a separate BNC jack to allow it to be triggered from an
external source. A low to high (+5 volts) transition on this BNC will trigger the internal
timer just as if the manual pushbutton had been pressed. The Picospritzer will not trigger
again until the signal is set to low. If a pulse train is applied to this jack, care should be
taken to ensure that the duration setting is less than the period of the pulse. Otherwise
pulses will be skipped.

4.5 REMOTE FOOT SWITCH (not included)

A jack on the front panel of the Picospritzer is provided for attachment of an optional
remote foot pedal for initiating a pulse. This convenience permits the investigator to be
some distance from the rack mount panel and to view an ejection through a microscope
while initiating the ejection. When using a remote foot switch, the foot switch has the
same function as the panel push button

4.6 EXTERNAL INPUT

For additional flexibility in experimental use, the Picospritzer III has a front panel BNC
jack for each channel which permits independent activation of the two channels from
external sources. The selector toggle switch above each jack determines the source of
the control signal for that channel. In the “EXTERNAL” position, it operates by energizing
the valve whenever the input signal is high (+5 V.D.C.) and de-energizes it when the
signal returns to ground. This allows for pulse durations longer than the capacity of the
internal timer. In the “TIMER” position, the channel is connected to the internal timer and
will activate whenever that timer is triggered (either manually or externally). The internal
timer length can only be adjusted manually.

4.7 SECOND CHANNEL

The Picospritzer III is equipped with a separate BNC jack and selector toggle for each of

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 the two channels. This allows 2 external signals to be used
to operate the 2 channels independently when the selector toggles are set to the
“EXTERNAL” position. Placing a selector toggle to the “TIMER” position connects that
channel to the internal timer for operation whenever the timer is triggered (either
manually or externally). To prevent a channel from triggering, place the selector switch in
the “EXTERNAL” position and do not make a connection to the BNC for that channel.

4.8 MARKER
The time mark provides a convenient indication of the duration of the pulse with respect
to a biological signal much like that of the “artifact” associated with iontophoresis. It is a
5-volt TTL signal controlled by channel one. The time mark not only provides a useful
indication of the duration of the pulse, but it may also serve as a sync-out signal for
triggering the sweep of an oscilloscope, etc.

4.9 PIPETTE CALIBRATION

Pipettes (not supplied by Parker Hannifin) are generally calibrated by ejecting water into
oil (generally mineral oil) and then measuring the droplet diameter with an optical
micrometer. Smaller droplets may cling to the tip of the pipette. This is the method
briefly discussed in the manual and from which the droplet chart is derived.

Since the duration setting is easier to use and more repeatable it is usually the one that
is varied. An operating pressure is selected and kept that way for a given
experiment. You may need to make a few test droplets at different pressures and
durations with a given pipette to determine what operating pressure gives you the
desired range by varying just the duration. When setting the pressure, it is important to
do so by increasing the value. If you over shoot your desired set point always back
down to less than that point and then raise it back up.

The volume ejected during a single pulse depends on the applied pressure, the pulse
duration, AND the pipette tip diameter. Every pipette must be calibrated by optically
measuring the droplet sizes that result from varying the pressure or duration.

A typical pipette will only hold 1 to 4 microliters of media. Doing one ejection in the
microliter range may be possible but multiple ejections are not. Doing multiple ejections
to total 1 to 2 microliters is possible but the user still must calibrate the system, know the
number of ejections that will be required to reach the desired volume, and keep track of
the number of ejections completed

5.0 BACKGROUND

In 1977, Dr. R.E. McCaman and associates provided a complete description (1) of a
pressure ejection system that utilized a high speed valve. This valve continues to be the
heart of the pressure system offering very precise control of ejection volumes (in the
picoliter range) and ejection times (in the millisecond range).

Furthermore, these investigators described a series of holders that permitted ejection

through micropipettes with sufficiently small tips that could be used for simultaneous
intracellular recordings during ejections.

These systems have been used for intracellular as well as extracellular ejections. In
listing advantages of the pressure system, these investigators emphasize that the linear

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 relationship between ejection volume and either duration of the pulse or of the applied
pressure permits a rapid, convenient and reliable calibration of each pipette (1, 3), unlike
that for electrophoretic techniques (7-9).

Pressure ejection seems an ideal approach to delivering uncharged substances such as

peptides (4, 6), steroids (4), and enzymes (2,5). The solutions used for pressure
ejections are usually several orders of magnitude more dilute than those used for
electrophoretic ejection (1, 3), thus avoiding receptor desensitization commonly
experienced with iontophoresis. The fact that the ejection efficiency of the pneumatic
systems is not influenced by solute concentration nor by net charge, makes them ideal
for intracellular injections of radiolabeled or tracer substances (13-15). Thus, pressure
systems have been used for intracellular injection of radiolabeled precursors or
neurotransmitters (10, 11) and [H3] –sugars as precursors of glycoproteins (12) in order
to study neuron-specific transmitter biosynthesis, axonal transport and cellular
topography. The reproducible and quantifiable ejections obtained with pressure systems
make them ideal for neuropharmacological studies of agonist and drug interactions with
membrane receptors (1, 3, 4).

As you find additional uses for your Picospritzer, please send us a reprint for addition to
our reference section so that others may benefit from your experience.

N.B.; H3=radioactivity (tritium) label substance.

6.0 REFERENCES

References describing the use and unique advantage of pressure systems in several
types of experimentation in the field of neurobiology, cell biology, and biophysics are:

1. McCaman, R.E., Mc Kenna, D.G. and Ono, J.K. “A pressure system for intracellular
and extracellular ejections of picoliter volumes.” Brian Research 136:141 (1977).

2. Sakaki, M., Sakai, H. and Woody, C.D. “Intracellular staining of cortical neurons by
pressure micro-injection of horseradish peroxidase and recovery by core biopsy.” Exp.
Neurol. 58:138 (1978)

3. Sakai, M., Swartz, B.E. and Woody, C.D. “Controlled micro release of
pharmacological agents: measurements of volume ejected in vitro through fine tipped
glass microelectrodes by pressure.” Neuropharmacol. 18:209 (1979)

4. Dufy, B., Vincent J-D. Fluery, H., Pasquier, P., Gourdji, D., and Tixler- Vidal, A.
“Membrane effects of thyrotropin-releasing hormone and estrogen shown by intracellular
recording from pituitary cells.” Science. 204.509 (1979)

5. Tauc, L., Hoffman, A., Tsuji, S., Hinzen, D. and Faille, L. “Transmission abolished in
a cholinergic synapse after injection of acetylcholinesterase into the presynaptic neuron.”
Nature, 250, 496 (1974)

6. Chang, J.J., Gelperin, A., and Johnson, F.H. “Intracellulary injected aequorin detects
transmembrane calcium flux during action potentials in an identified neuron from the
terrestrial slug.” Brain Research 77:431 (1974)

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 7. Krnjevic, K., Mitchell, J.F., and Szerb, J.C. “Determination of iontophoretic release of
acetylcholine from micropipettes.” J.Physiol. 165:421 (1963)

8. Zieglgansberger, W., Southmann, G., and Herz, A. “Iontophoretic release of

substances from micropipettes in vitro.” Neuropharmacol. 13:417 (1974)

9. Kuffler, S. and Yoshikami, D. “The number of transmitter molecules in a quantum: an

estimate from iontophoretic application of acetylcholine at the neuromuscular synapse.”
J. Physiol. 251:465 (1975)

10. Eisenstadt, M., Goldman, J.E., Kandel, E.R., Koike, H., Koester, J. and Schwartz, J.
“Intrasomatic injection of radioactive precursors for studying transmitter synthesis in
identified neurons of Aplysia.” Proc. Nat. Acad. Sci. 70:3371 (1973)

11. Schwartz, J.H. “Synthesis, axonal transport and release of acetylcholine by identified
neurons of Aplysia.” Proc. Nat. Acad. Sci. 70:3371 (1973)

12. Thompson, E.B., Schwartz, J.H. and Kandel, E.R. “A radio autographic analysis in
the light and electron microscope of identified Aplysia neurons and their processes after
intrasomatic injection of L-H3-Fucose.” Brain Research.112:251 (1976)

13. Baux, G., Simonneau, M. Tauc, L. “Transmitter release, Ruthenium red used to
demonstrate a possible role of sialic acid containing substrates.” J. Physiol. 291, 161-
178 (1979)

14. Sakai, M., Sakai, H., and Woody, C.D. “Sampling distribution of morphologically
identified neurons of coronal- pericruciate cortex of awake cats following intracellular
injection of HRP.” Brain Research. 152: 329-333 (1978)

15. Amaral, D.G. and Price, J.L. “An air pressure system for the injection of tracer
substances into the brain.” Jrnl. Neuroscience Methods. 9:35-34 (1983)

16. Waitzman, D.M., Sliakov, V.L., DePalma-Bowles, S. and Ayers, A.S. “Effects of
reversible inactivation of the primate mesencephalic reticular formation. I. Hypermetric
goal-directed saccades.” Journal of Neurophysiology (2000)
https://journals.physiology.org/doi/full/10.1152/jn.2000.83.4.2260

17. Pham, L.N., Dionne, M.S., Shiarsu-Hiza, M. and Schneider, D.S. “A specific primed
immune response in drosophila is dependent on phagocytes.” PLOS
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.0030026 (2007)

18. Kim, L.K., Choi, U.Y., Cho, H.S., Lee, J.S., Lee, W., Kim, J., Jenog, K., Shim, J.,
Kim, J. and Kim, Y. “Down-regulation of NF-κB target genes by the AP-1 and STAT
complex during the innate immune response in drosophila.” PLOS
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0050238 (2007)

19. Kuhlman, S.J. and Huang, Z.J. “High-resolution labeling and functional manipulation
of specific neuron types in mouse brain by cre-activated viral gene expression.” PLOS
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0002005 (2008)

20. Gibbs, G.M., Orta, G., Reddy, T., Koppers, A.J., Martinez-Lopez, P., Vega-Beltran,

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 J.L., Lo, J.C.Y., Veldhuis, N., Jamsai, D., McIntyre, P., Darszon, A. and O’Bryan, M.K.
“Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a
role in establishing sperm function.” University of Hawaii
https://www.pnas.org/content/108/17/7034.figures-only (2011)

FIGURE 1: DROPLET CALIBRATION CHART

Calibration chart provided courtesy of Dr. Joyce K. Ono, Department of Biological

Science, California State University

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FIGURE 2: CHARACTERISTICS OF THE PRESSURE EJECTION SYSTEM

The diameter of the droplet ejected in air was measured with an ocular micrometer in a
dissecting microscope. The volume was calculated for droplets formed by varying pressure or
pulse duration parameters. The following graphs demonstrate that each pipette can be
calibrated by varying these two major determinants of the volume ejected.

FIGURE 2A: LINEARITY OF VOLUME EJECTED WITH VARYING PULSE DURATION

AT CONSTANT PRESSURE

The X-intercept (15 MSEC) represents the mechanical lag time of the particular solenoid
valve.

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FIGURE 2B: LINEARITY OF VOLUME EJECTED WITH VARYING PRESSURE AT
CONSTANT PULSE, DURATION

The X-intercept (7.5PSI) represents the minimum pressure necessary for ejection and is a
characteristic of a particular pipette.

FIGURE 2C: LINEARITY OF THE AMOUNT OF AN H3 STANDARD WITH VARIOUS

EJECTED VOLUMES

The points of this graph are highly correlated (R=.97) with the independently determined
specific activity (6.5 x 106 CPM/mL) of the radioactive solution.

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FIGURE 3: COMPARISONS OF RESPONSES OF APLYSIA CALIFORNICA NEURONS TO
IONTOPHORETIC AND PRESURE APPLICATION OF COMPOUNDS

FIGURE 3A:

Superimposed traces of Aplysia buccal neuron responses to ACh delivered by an iontophoretic

pulse (1µ amp, 80 MSEC) and a pressure pulse (40 PSI, 60 MSEC, 60 µm diameter droplets of
10-3 M ACh). The amplitude and polarity of the pressure artifacts (negative square pulse in this
case) can be manipulated.

FIGURE 3B:

Comparisons of responses to pressure ejected acetylcholine (ACh) in Aplysia neuron in the

absence (control) and presence of an iontophoretic pipette (70 MEGOHMS) containing 1 M ACh.
The dose-responsive curve is shifted to the right because of desensitization from ACh leaking
out of iontophoretic pipette. Each point is the mean response + standard error of the mean.

Problems of desensitization can be circumvented with a pressure pipette since the pipette is
usually filled with agonists in the concentrations of 10-6 to 10-3 M in contrast to iontophoretic
pipettes. Braking current is not necessary for the pressure pipette, thus avoiding inconsistent
ejection of compounds.

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FIGURE 3C:

Reproducibility of 12 consecutive responses to acetylcholine (ACh) delivered by a pressure

pulse to an Aplysia (Sea Hare) neuron. A 10-4M solution of ACh was ejected by 22 PSI for 5
MSEC to produce a droplet estimated to be 10 PL (=1 Femtomole of ACh). Calibration: 2 mV,
.02 Sec.

FIGURE 3D:

Comparison of response to iontophoretic (I) and pressure (P) applied ACh in a drug study. Both
responses are equally antagonized by hexamenthonium, even though the ACh in the pressure
pipette is ejected in a droplet of normal artificial seawater. Similar results are obtained during
substitution experiments. The arrows in the figure point to an input resistance test pulse.

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7.0 PICOSPRITZER III FAQ (Frequently Asked Questions):

1. I used to purchase the vacuum loading unit is that still available?

The vacuum loading unit is no longer available for purchase.

2. I used to purchase a 0-10, 0-30 or 0-60psi regulator, are those options still available?

The 0-10, 0-30, or 0-60psi regulator options are no longer available, only the 0-100psi
configuration is available. Please see Appendix 1 for alternative regulator information.

3. Can I trigger the two channels independently using two separate footswitches?

The Picospritzer III can only handle one footswitch (the footswitch controls the internal timer).
You can trigger them independently using an external trigger. Please see Section 4.7 Second
Channel for more details.

4. Can I provide separate pressures to the two valve boxes?

The Picospritzer III will only provide the same pressure to both valve boxes.

5. What can I do if my system is experiencing an issue with backflow?

Check the capillary tip size. Typically, we recommend a tip size between 1 and 5 microns. Once
the resistance of the tip is higher than the capillary effect of the pipette, material will no longer
be drawn into the pipette. Keep in mind that other factors such as pressure, timing, and the
nature of the material being ejected, may come into play.

6. What is the shortest pulse duration the Picospritzer III can support?

A minimum of 3 milliseconds should be used to ensure the valve in the valve box can fully
actuate for injection.

7. What are the approximate shipping weights and dimensions of the Picospritzer III

The Picospritzer III typically ships in a box with dimensions of 24” x 14” x 9” and weighs
approximately 10 lbs. Actual shipping weights and dimensions may vary.

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APPENDIX 1 – PART INFORMATION

Available from Parker Hannifin

PICOSPRITZER® III Two Channel Unit (0 – 100 psi regulator)

PARKER HANNIFIN P/N 052-0500-900

VALVE BOX & CABLE ASSEMBLY

PARKER HANNIFIN P/N 051-0009-401-1

Commercially Available from Parker Hannifin Distributors (except as noted*)

ALTERNATIVE REGULATORS:

Regulator Regulator Regulator P/N Pressure Gauge Gauge P/N

Range Vendor Vendor
0-100 psig Parker Watts R374-01C Wika* 9690234
0-60 psig Parker Watts R374-01B Parker Wilkerson K4515N18060
0-30 psig Parker Watts R374-01A Parker Wilkerson K4515N18030
0-10 psig Parker Watts R374-01AX43 Ametek* 146010

Commercially Available (Not Sold by Parker Hannifin)

PIPETTE HOLDERS:

Available from Warner Instruments (www.warneronline.com)

PIPETTES:

Available from Eppendorf (www.eppendorf.com)

TUBING AND FITTINGS:

Picospritzer to Valve Boxes

Tubing: PTFE Teflon 1/8” OD, .062” ID
Nut: 1/4-28 THD for 1/8” OD Tubing
Ferrule: for 1/8” OD Tubing

Valve Boxes to Pipette Holders

Tubing: PTFE Teflon 1/16” OD, .031” ID
Nut: 1/4-28 THD for 1/16” OD Tubing
Ferrule: for 1/16” OD Tubing

FOOTSWITCH:

StealthSwitch FS-2 available from StealthSwitch (www.stealthswitch.com)

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APPENDIX 2 – DRAWINGS

Picospritzer III – Pneumatic Assembly

Regulator Assembly Schematic

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