HERAcell 150i 240i With Decontamination Routine PDF

Operating Instructions
CO2 Incubator
HERAcell® 150i / 240i
with Decontamination Routine
50115191 B / 11.2010
Copyright 2009 Thermo Fisher Scientific. All rights reserved.
These operating instructions are protected by copyright. Rights resulting
thereof, particularly reprint, photomechanical or digital postprocessing or
reproduction, even in part, are only allowed with the written consent of the
Thermo Electron LED GmbH.
This regulation does not apply to reproductions for in-plant use.
The contents of the operating instructions are subject to change without further
notice. Concerning translations into foreign languages, the German version of
these operating instructions is binding.
Trademarks
HERAcell®, iCan™ and iONGUARD™are registered trademarks of Thermo Scientific.
Thermo Scientific is a brand of Thermo Fisher Scientific Inc.
All trademarks mentioned in the operating instructions are the exclusive property
of the respective manufacturers.
Thermo Electron LED GmbH

Robert-Bosch-Straße 1
D - 63505 Langenselbold
Germany
Thermo Electron LED GmbH is a subsidiary company of:

Thermo Fisher Scientific Inc.
81 Wyman Street
Waltham, MA 02454
USA
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Operating Instructions | HERAcell 150i / 240i
Contents
1 General notes 9
1.1 Identification of the device and of the documentation....................................... 10
1.2 Instruction of the operating personnel................................................................ 10
1.3 Applicability of the instructions ........................................................................... 11
1.4 Warranty ............................................................................................................... 11
1.5 Explanation of safety information and symbols ................................................. 12
1.5.1 Safety information and symbols used in the operating instructions........... 12
1.5.2 Additional symbols for safety informations: ................................................ 12
1.5.3 Symbols on the device .................................................................................. 13
1.6 Intended purpose of the device........................................................................... 14
1.6.1 Correct use ..................................................................................................... 14
1.6.2 Incorrect use................................................................................................... 14
1.7 Standards and directives ..................................................................................... 15
1.8 Safety notes on gases.......................................................................................... 16
1.8.1 Safety notes on carbon dioxide (CO2) .......................................................... 16
1.8.2 Safety notes on oxygen (O2) ......................................................................... 17
1.8.3 Safety notes on nitrogen (N2) ........................................................................ 17
2 Delivery of the device 18

2.1 Packaging ............................................................................................................. 18
2.2 Acceptance inspection......................................................................................... 18
2.3 Standard equipment components HERAcell® 150i............................................ 19
2.4 Optional equipment components HERAcell® 150i ............................................. 19
2.5 Standard equipment components HERAcell® 240i............................................ 20
2.6 Optional equipment components HERAcell® 240i ............................................. 21
3 Installation of the device 22

3.1 Ambient conditions.............................................................................................. 22
3.2 Room ventilation .................................................................................................. 22
3.3 Space requirements ............................................................................................. 23
3.4 Transport .............................................................................................................. 24
3.5 Stacking ................................................................................................................ 24
3.6 Retrofitting/Modifications .................................................................................... 25
4 Description of the device 26

4.1 Front view of HERAcell® 150i .............................................................................. 26
4.2 Rear view HERAcell® 150i ................................................................................... 28
4.3 Front view HERAcell® 240i .................................................................................. 32
4.4 Rear view HERAcell® 240i ................................................................................... 34
4.5 Safety devices ...................................................................................................... 38
4.6 Work space atmosphere ...................................................................................... 38
4.7 Door switch .......................................................................................................... 40
4.8 Sensor system...................................................................................................... 41
4.9 Supply interface ................................................................................................... 42
4.10 Work space components ................................................................................... 44
4.10.1 Interior container.......................................................................................... 44
4.10.2 Optional split gas-tight screens ................................................................... 44
4.10.3 Water reservoir............................................................................................. 45
4.10.4 Gas humidification (optional, only for O2 control)...................................... 45
4.10.5 Heating system............................................................................................. 45
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4.10.6 Rear panel openings .................................................................................... 46

4.10.7 Shelf system ................................................................................................. 47
4.10.8 Bottle turning device for HERAcell® 240i (optional) .................................. 47
4.10.9 Water pump.................................................................................................. 48
5 Start-up 49
5.1 Let the device adapt to the ambient conditions ................................................. 49
5.2 Preparing the work space .................................................................................... 49
5.3 Installing the shelf system ................................................................................... 50
5.3.1 Installation/removal of the support rail ......................................................... 50
5.3.2 Installing the shelf supports: ......................................................................... 51
5.3.3 Levelling the device ....................................................................................... 51
5.4 Installing the gas humidification (optional, only for O2/N2-control)................... 51
5.5 Installing the optional bottle turning devices HERAcell® 240i ........................... 52
5.6 Installing the optional center strut HERAcell® 240i for split inserts................... 54
5.7 Gas connection..................................................................................................... 55
5.7.1 Installing gas pressure hoses ........................................................................ 55
5.7.2 Installing devices without the optional gas monitoring system .................. 56
5.7.3 CO2 connection with gas monitoring system (optional) .............................. 57
5.7.4 Combined CO2/O2/N2 connection with gas monitoring system (optional) . 57
5.8 Power supply connection .................................................................................... 59
5.9 Connecting the RS 232 interface ......................................................................... 60
5.10 Connecting the USB interface (optional)........................................................... 60
5.11 Connecting the alarm contact............................................................................ 61
6 Operation 63
6.1 Preparing the device ............................................................................................ 63
6.2 Starting operation ................................................................................................ 64
7 Handling and control (iCan™ touchscreen controller) 65

7.1 Power switch ........................................................................................................ 65
7.2 Operating panel and operating structure............................................................ 65
7.3 Factory presettings of the iCan™ touchscreen controls..................................... 68
7.4 Heat-up phase of the control loop sensors......................................................... 68
7.5 Event actions for settings .................................................................................... 69
7.6 Setting the temperature set value ....................................................................... 70
7.7 Setting the CO2 set value..................................................................................... 71
7.8 Setting the O2 set value ....................................................................................... 72
7.9 Auto-start routine ................................................................................................. 73
7.9.1 Activating auto-start....................................................................................... 75
7.9.2 Interrupting the auto-start routine ................................................................. 76
7.10 Running the contra-con routine......................................................................... 76
7.11 User configuration.............................................................................................. 76
7.11.1 Settings......................................................................................................... 77
7.11.2 Event logging ............................................................................................... 83
7.11.3 Options ......................................................................................................... 85
7.11.4 Icon description............................................................................................ 90
7.11.5 Enabling/disabling the keypad lock............................................................. 92
7.11.6 Software versions ........................................................................................ 93
7.12 Scaling the trend display ................................................................................... 94
7.13 Error messages .................................................................................................. 95
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7.13.1 Response to an error message event ......................................................... 95

7.13.2 Resetting the overtemperature protection mechanism ............................. 96
7.13.3 Troubleshooting ........................................................................................... 96
8 Shut-down 100
8.1 Shutting the device down.................................................................................. 100
9 Cleaning and disinfection 101

9.1 Cleaning .............................................................................................................. 101
9.2 Decontamimation procedures ........................................................................... 102
9.3 Wipe/spray disinfection ..................................................................................... 102
9.4 contra-con decontamination routine................................................................. 105
9.4.1 Activating the contra-con routine................................................................ 108
9.4.2 Interrupting contra-con ................................................................................ 108
9.4.3 contra-con interruption due to error ........................................................... 109
9.4.4 Completing contra-con ................................................................................ 110
10 Maintenance 111
10.1 Inspections and checks.................................................................................... 111
10.2 Service intervals ............................................................................................... 111
10.3 Preparing the temperature calibration ............................................................ 112
10.4 Temperature calibration procedure ................................................................ 113
10.5 Preparing the CO2 calibration .......................................................................... 114
10.6 CO2 calibration procedure ............................................................................... 116
10.7 Replacing the gas inlet filter............................................................................. 117
10.8 Replacing the device fuses .............................................................................. 117
10.9 Replacing the door seal ................................................................................... 118
11 Disposal 119
12 Technical data 121
13 Annex: Data communication 125

13.1 Interfaces .......................................................................................................... 125
13.1.1 RS 232 interface ......................................................................................... 125
13.1.2 USB port (optional) .................................................................................... 125
13.1.3 Installing the USB port driver .................................................................... 126
13.2 Structure of the command sequences for data communication ................... 128
13.2.1 Description of the protocol ........................................................................ 128
13.3 Overview of general parameters (addresses 0xxx) ........................................ 129
13.3.1 Reading general parameters ..................................................................... 129
13.4 Overview of incubator parameters (addresses 2xxx)..................................... 130
13.4.1 Reading basic parameters ......................................................................... 130
13.4.2 Reading internal function parameters ....................................................... 131
13.5 Error memory structure ................................................................................... 133
13.5.1 Error memory data set structure scheme:................................................ 134
13.5.2 Overview of the possible error messages in hex coding......................... 134
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13.5.3 Overview of the possible error messages in bit coding........................... 135

13.6 Data logger structure ....................................................................................... 137
13.6.1 Data logger data set structure scheme:.................................................... 138
13.6.2 Overview of possible event entries in bit coding ..................................... 139
13.7 Examples of data logger codes ....................................................................... 141
13.7.1 Functions for data logger queries ............................................................. 141
13.7.2 Example of a code for a a data logger query............................................ 141
13.8 Program Heracell 150i & 240i........................................................................... 146
13.8.1 Installing the Heracell 150i & 240i.............................................................. 147
13.8.2 Operating the Heracell 150i & 240i ............................................................ 148
14 Device log 155
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Betriebsanleitung | HERAcell 150i / 240i
Figures
Fig. 1: Device dimensions.................................................................................... 23

Fig. 2: Lift points ................................................................................................... 24
Fig. 3: Stacking ..................................................................................................... 24
Fig. 4: Front view HERAcell® 150i ....................................................................... 27
Fig. 5: Rear view HERAcell® 150i ........................................................................ 29
Fig. 6: Looped-through gas supply HERAcell® 150i........................................... 31
Fig. 7: Front view HERAcell® 240i ....................................................................... 33
Fig. 8: Rear view HERAcell® 240i ........................................................................ 35
Fig. 9: Looped-through gas supply HERAcell® 240i........................................... 37
Fig. 10: Door switch ............................................................................................... 40
Fig. 11: Temperature, CO2,and O2 sensors .......................................................... 41
Fig. 12: Supply interface ........................................................................................ 42
Fig. 13: Work space components .......................................................................... 44
Fig. 14: Water reservoir.......................................................................................... 45
Fig. 15: Rear panel openings ................................................................................. 46
Fig. 16: Bottle turning device................................................................................. 47
Fig. 17: Water pump............................................................................................... 48
Fig. 18: Support rail installation/removal .............................................................. 50
Fig. 19: Shelf support installation .......................................................................... 51
Fig. 20: Installing gas humidification ..................................................................... 51
Fig. 21: Installing the bottle turning devices ......................................................... 52
Fig. 22: Bottle turning speed.................................................................................. 53
Fig. 23: Center strut installation............................................................................. 54
Fig. 24: Installing gas pressure hoses ................................................................... 55
Fig. 25: Gas connection without gas monitoring system..................................... 56
Fig. 26: Gas connection with gas monitoring system (optional) ......................... 57
Fig. 27: Combined connection with gas monitoring system (optional)............... 58
Fig. 28: Combined gas connection with gas monitoring system......................... 59
Fig. 28: Power supply connection ......................................................................... 59
Fig. 29: Connection example ................................................................................. 62
Fig. 30: Power switch ............................................................................................. 65
Fig. 31: Pressure-sensitive areas ........................................................................... 65
Fig. 32: iCan™ touchscreen withoutO2/N2gas supply .......................................... 66
Fig. 33: iCan™ touchscreen with combined gas connection ............................... 66
Fig. 34: Menu structure overview.......................................................................... 67
Fig. 35: Heat-up phase display .............................................................................. 68
Fig. 36: Setting the temperature set value ............................................................ 70
Fig. 37: Setting the CO2 set value.......................................................................... 71
Fig. 38: Setting the O2 set value ............................................................................ 72
Fig. 39: Activating the auto-start routine............................................................... 75
Fig. 40: Interrupting the auto-start routine ............................................................ 76
Fig. 41: Changing the code .................................................................................... 77
Fig. 42: Date / time setting ..................................................................................... 78
Fig. 43: Display contrast setting ............................................................................ 79
Fig. 44: Key tone setting ........................................................................................ 79
Fig. 45: RS 232 interface baud rate setting ........................................................... 80
Fig. 46: USB interface baud rate setting................................................................ 80
Fig. 47: Language setting....................................................................................... 81
Fig. 48: Reminder interval setting.......................................................................... 82
Fig. 49: Event display ............................................................................................. 83
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Betriebsanleitung | HERAcell 150i / 240i
Figures
Fig. 50: Logging cycle setting ................................................................................ 83

Fig. 51: Error table display ..................................................................................... 84
Fig. 52: Alarm relay setting .................................................................................... 85
Fig. 53: Low humidity setting ................................................................................ 86
Fig. 54: Gas tight screen setting ............................................................................ 87
Fig. 55: Water level setting .................................................................................... 87
Fig. 56: Alarm relay setting .................................................................................... 88
Fig. 57: Bottle turning device speed setting.......................................................... 88
Fig. 58: Switching the O2 control on and off......................................................... 89
Fig. 59: Icon description overview ........................................................................ 90
Fig. 60: Gas monitoring icons................................................................................ 91
Fig. 61: Enabling/disabling the keypad lock.......................................................... 92
Fig. 62: Software versions ..................................................................................... 93
Fig. 63: Trend display scaling ................................................................................ 94
Fig. 64: Event error messages ............................................................................... 95
Fig. 65: Event error overtemperature.................................................................... 96
Fig. 66: Decontamination routine phases ........................................................... 107
Fig. 67: Activating contra-con.............................................................................. 108
Fig. 68: Interrupting / cancelling contra-con ....................................................... 109
Fig. 69: contra-con completion............................................................................ 110
Fig. 70: Preparing the temperature calibration ................................................... 112
Fig. 71: Temperature calibration procedure ....................................................... 113
Fig. 72: Preparing CO2 calibration ....................................................................... 114
Fig. 73: Gas-tight screen measurement opening............................................... 115
Fig. 74: CO2 calibration procedure ...................................................................... 116
Fig. 75: Gas inlet filter installation........................................................................ 117
Fig. 76: Device fuse replacement ........................................................................ 117
Fig. 77: Magnetic door seal replacement............................................................ 118
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1 General notes
Overview of the Thermo Fisher international sales organisations
Europe, Middle East, Africa Postal address Germany:

Thermo Electron LED GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Enquiries from Germany:

Phone Sales 0800 1 536376
Phone Service 0800 1 112110
Fax Sales/Service 0800 1 112114
E-Mail [email protected]
Enquiries from Europe, Middle East, and Africa:

Phone + 49 (0) 6184 / 90-6940
Fax + 49 (0) 6184 / 90-6772
America Postal address USA:

Thermo Fisher Scientific
275 Aiken Road
Asheville, NC 28804
USA
Enquiries from North America:

Phone +1 800-879 7767
Fax +1 828-658 0363
Enquiries from Latin America:

Phone +1 828-658 2711
Fax +1 828-645 9466
Enquiries from Asia Pacific:

Phone +852-2711 3910
Fax +852-2711 3858
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1 General notes
1.1 Identification of the device and of the documentation
Identification of the device

Identfication data Device name: CO2 incubator
Type designation: HERAcell® 150i
HERAcell® 240i
Serial No.: 40830469 or higher
Allocation of product documentation

User information: Operating instructions 50113925 E
Valid: 11.2010
Certifications and quality audit:

Certification: CE Certification
Test mark: GS by VDE Germany
CSA/UL by VDE Germany
1.2 Instruction of the operating personnel
These operating instructions describe the CO2 incubator HERAcell® 150i /

240i. The CO2 incubator has been manufactured in keeping with the latest
technological developments and is operationally safe. However, the device
may present potential hazards, particularly if it is operated by inadequately
trained personnel or if it is not used in accordance with the intended
purpose. Therefore, the following must be observed to prevent accidents:
Target group • The CO2 incubator must be operated only by trained and authorized
personnel.
Process procedures for personnel • For any operation of this device, the operator must prepare clear and
concise written instructions in the language of the operating and
cleaning personnel based on these operating instructions, applicable
safety data sheets, plant hygiene guidelines, and technical regulations,
in particular:
– which decontamination measures are to be applied for the CO2
incubator and accessories,
– which protective measures apply when specific agents are used,
– which measures are to be taken in the case of an accident.
• Repairs to the device must be carried out only by trained and
authorized expert personnel.
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1 General notes
1.3 Applicability of the instructions
Original document for translation • The contents of the operating instructions are subject to change
without further notice.
• Concerning translations into foreign languages, the German version of
these operating instructions is binding.
• Keep these operating instructions close to the device so that safety
instructions and important information are always accessible.
Should you encounter problems that are not detailed adequately in these
operating instructions, please contact Thermo Scientific immediately for
your own safety.
1.4 Warranty
Requirements for warranty Thermo Scientific warrants the operational safety and functions of the CO2
incubator only under the condition that:
• the device is operated and serviced exclusively in accordance with its
intended purpose and as described in these operating instructions,
• the device is not modified,
• only original spare parts and accessories that have been approved by
Thermo Fisher Scientific are used,
• inspections and maintenance are performed at the specified intervals.
The warranty is valid from the date of delivery of the device to the operator.
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1 General notes
1.5 Explanation of safety information and symbols
1.5.1 Safety information and symbols used in the operating

instructions
WARNING: Personal injuries
Indicates a hazardous situation which, if not avoided, could

result in death or serious injuries.
CAUTION: Personal injuries
Indicates a hazardous situation which, if not avoided, could

result in minor or moderate injuries.
NOTICE: Property damage

Indicates a situation which, if not avoided, could result in
property damage.
NOTE: Useful information
Is used for applicational hints and useful information.
1.5.2 Additional symbols for safety informations:
Wear safety gloves!
Wear safety goggles!
Harmful liquids!
Electric shock!
Hot surfaces!
Fire hazard!
Explosion hazard!
Suffocation hazard!
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1 General notes
1.5.3 Symbols on the device
CE conformity mark: confirms conformity according to EU Guidelines
H VDE test mark
Mark of conformity USA/Canada
Observe operating instructions!
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1 General notes
1.6 Intended purpose of the device
1.6.1 Correct use

Use The CO2 incubator HERAcell® 150i / 240i is a laboratory device for
preparing and cultivating cell and tissue cultures. The device allows the
simulation of the special physiological ambient conditions for these
cultures due to the exact control of:
• temperature,
• CO2 content,
• O2/N2 content,
• and increased relative humidity.
Fields of application HERAcell® 150i / 240i has been designed for installation and operation in
the following fields of application:
• Laboratories for cytobiological and biotechnological experiments of
safety levels L1, L2, and L3.
• Medical-microbiological laboratories in accordance with DIN 58 956.
• Laboratories in the central area of clinics and hospitals.
Gas supply system The gases required for the incubator (CO2 and / or O2/N2) are supplied to
the device from a separate gas supply system, either from gas cylinders or
from a central pressurized gas container.
The layout of the gas supply system must ensure that the operating
pressure of the gas supply lines can be set to a range between 0.8 bar
(min.) to 1 bar (max.) and that the pressure cannot be changed.
If the device is equipped with the optional gas monitoring system, up to
four devices can be interconnected in line, independent of the of the gas
supply system capacity.
The CO2 incubator is suitable for continuous operation.
1.6.2 Incorrect use

Incorrect use Do not use cell or tissue cultures in the device that are not in accordance
with the regulations of safety levels L1, L2, and L3.
Do not use tissues, substances or liquids that:
• are easily ignitible or explosive,
• release vapors that form combustible or explosive mixtures when
exposed to air,
• release poisons.
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1 General notes
1.7 Standards and directives
The device complies with the following standards and guidelines:

• DIN EN 61010 - 1: 2002, DIN EN 61010 - 2 - 010 : 2004
• Low Voltage Guideline 2006/95/EG
• EMC Guideline 2004/108/EG
• UVV BGV D4 Revised Version
(UVV VBG 20, continues to be valid for old stock)
The following safety regulations must be observed if the device is operated
within the territory of the Federal Republic of Germany:
• BGR 104
• BGR 120
• BGI 629
• BGI 630
• TRG 280
• EC Official Gazette, L 374
• Safety data sheets of the gas suppliers with regard to the particular
characteristics of CO2, O2, and N2.
• Principles of good microbiological proceedings, notice of the trade
association of the German chemical industry.
For other countries, the applicable national regulations are binding.
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1 General notes
1.8 Safety notes on gases
Installation work:
Any work to supply lines and pressurized gas containers,
cylinders or containers used for storing CO2 or O2/N2 must only
be carried out by expert personnel using the appropriate tools.
Instruction of the personnel:

Personnel operating devices with CO2 supply must be
instructed about the particularities in the handling of CO2
before starting their work:
• Correct operation of pressurized gas containers and gas
supply systems,
• obligation to report damages and shortcomings in CO2
supply lines,
• measures to be taken in case of accidents or failures.
These instructions must be repeated at appropriate intervals and must

comprise the particular operating instructions of the gas supplier.
1.8.1 Safety notes on carbon dioxide (CO2)

Carbon dioxide Since CO2 is rated as a harmful gas, certain safety instructions must be
observed when the CO2 incubator is started up and when the device is
operated.
Suffocation hazard!
Large amounts of CO2 released into the room atmosphere may
cause suffocation.
If CO2 is released, initiate safety measures immediately!
• Leave the room immediately and do not allow others to
enter the room!
• Inform security service or fire department!
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1 General notes
1.8.2 Safety notes on oxygen (O 2)

Oxygen O2 is a gas that promotes combustion and may explode in combination
with grease-containing materials.
Oxygen explosion!
O2 may explode in combination with oils, greases, and
lubricants. If highly compressed oxygen comes in contact with
grease- or oil-containing substances, the mixture may explode!
• For cleaning these device components, use only oil- and
grease-free lubricants.
• Keep all connections and components of the oxygen system
free from substances that contain oil, grease, or lubricant!
Fire hazard!
Released oxygen promotes combustion. Do not use open
flames in the vicinity of oxygen-operated systems!
• Do not smoke in the vicinity of oxygen systems.
• Do not expose the components of an oxygen system to
excessive heat.
1.8.3 Safety notes on nitrogen (N 2)

Nitrogen Nitrogen mixes easily with air. High concentrations of nitrogen reduce the
oxygen content in the air.
Suffocation hazard!
Large amounts of N2 released into the room atmosphere may
cause suffocation.
If N2 is released, initiate safety measures immediately!
• Leave the room immediately and do not allow others to
enter the room!
• Inform security service or fire department!
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2 Delivery of the device
2.1 Packaging
The CO2 incubator HERAcell® 150i / 240i is delivered in a stable packaging

box. All packaging materials can be separated and are reusable:
Packaging materials • Packaging carton:
Recycled paper
• Foam elements:
Styrofoam (CFC-free)
• Pallet:
Untreated wood
• Packaging film:
Polyethylene
• Packaging ribbons:
Polypropylene
2.2 Acceptance inspection
After the device has been delivered, check the delivery immediately for:
• completeness,
• possible damage.
Damage report If components are missing or damage is found on the device or the
packaging, esp. damages caused by humidity and water, please contact
the linehauler as well as the Technical Support of Thermo Scientific
immediately.
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2.3 Standard equipment components HERAcell® 150i
HERAcell® 150i
Quantity of the delivered components CO2 incubator or CO2/O2 incubator
(in piece) Solid glass door and 3-door gas tight screen and
continuous shelves continuous shelves
Shelf 3 3
Support rail for shelf 4 4
Shelf supports for shelf 6 6
Insert for pressure compensation opening 1 1
Plug for pipe channel 1 1
Power supply cable 1 1
Connector, potential-free contact 1 1
Spare caps, set 1 1
CO2 connecting hose set 1 1
Open-end wrench, 24 mm 1 1
Allen wrench 2 mm for blower wheel 1 1
Allen wrench 3 mm for blower wheel cover 1 1
Operating instructions (CD) 1 1
Water pump 1 1
2.4 Optional equipment components HERAcell® 150i
HERAcell® 150i
Quantity of the delivered components CO2 incubator or CO2/O2 incubator
(in piece) Solid glass door and 3-door gas tight screen and
continuous shelves continuous shelves
O2 connecting hose set 1 1
O2 sensor with set for gas humidification *1) 1 1
CO2 connecting hose set, gas monitoring (optional) 1 1
O2 / N2 connecting hose set, gas monitoring (optional) 1 1
*1) O2 sensor with gas humidification set is packed in a separate carton and placed into
the work space during transport.
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2.5 Standard equipment components HERAcell® 240i
HERAcell® 240i
CO2 incubator or CO2/O2 incubator
Quantity of the delivered
Solid glass door and Solid glass door 6-door gas tight 6-door gas tight
components
continuous shelves and split shelves screen and screen and split
(in piece)
(standard version) (optional) continuous shelves shelves
(standard version) (optional)
Shelf 3 6 3 6
Support rail for shelf 4 6 4 6
Shelf supports for shelf 6 12 6 12
Insert for pressure
1 1 1 1
compensation opening
Plug for pipe channel 1 1 1 1
Power supply cable 1 1 1 1
Connector, potential-free
1 1 1 1
contact
Spare caps, set 1 1 1 1
CO2 connecting hose set 1 1 1 1
Open-end wrench, 24 mm 1 1 1 1
Allen wrench 2 mm for
1 1 1 1
blower wheel
Allen wrench 3 mm for
1 1 1 1
blower wheel cover
Operating instructions (CD) 1 1 1 1
Water pump 1 1 1 1
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2.6 Optional equipment components HERAcell® 240i
HERAcell® 240i
CO2 incubator or CO2/O2 incubator
Quantity of the delivered
components Solid glass door and Solid glass door 6-door gas tight 6-door gas tight
(in piece) continuous shelves and split shelves screen and screen and split
(standard version) (optional) continuous shelves shelves
(standard version) (optional)
O2 connecting
1 1 1 1
hose set
O2 gas humidification
system connecting hose 1 1 1 1
set *1)
Bottle turning device
(motor with four 1 — 1 —
rollers, optional) *2), *3)
CO2 connecting hose set,
1 1 1 1
gas monitoring (optional)
O2/ N2 connecting hose set,
1 1 1 1
gas monitoring (optional)
*1) O2 sensor with gas humidification set is packed in a separate box that is placed into
the work space during transport.
*2) For bottle turning devices, the version with split shelves is not available.
*3) Each level of a bottle turning device is packed in a separate box. The boxes (1 to 4)
are delivered with the device.
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3 Installation of the device
3.1 Ambient conditions
The device must only be operated at locations that meet the particular
ambient conditions listed below:
Requirements:
Location • Draft-free and dry location.
• The minimal distance to adjacent surfaces must be observed on all
sides (see Section 3.3.).
• The operating room must be equipped with appropriate room
ventilation.
• Solid, level, fire-proof surface.
• Vibration-proof substructure (floor stand, lab table) capable of bearing
the weight of the device and of accessories (particularly if several
devices are stacked).
• The electrical system of the device has been designed for an operating
height of up to 2000 m above sea level.
• To ensure a constant incubation temperature of 37 °C, the ambient
temperature must be within a range of +18 °C to +33 °C.
• Relative humidity up to 80% (max.).
• No direct exposure to sunlight.
• Devices that produce excessive heat must not be placed near the
location of the HERAcell® 150i / 240i.
3.2 Room ventilation
When CO2/O2/N2 is supplied, the work space of the incubator is slightly

pressurized. The pressure is released through the pressure compensation
opening into the operating room.
Pressure compensation and any opening of the glass door/gas-tight screen
during the operation of the device will release very small quantities of CO2/
O2/N2 into the operating room; the room ventilation must be capable of
carrying the released gas safely off into the open.
In addition, heat dissipating from the device during continuous operation
may cause a change in the room climate.
• Therefore, the HERAcell® 150i / 240i must only be installed in rooms
with sufficient ventilation.
• Do not install the device in room recesses without ventilation.
Technical ventilation • The room ventilation should be a technical ventilation that complies
with the requirements of BGR 120 (Guidelines for laboritories,
Germany) or some other suited ventilation system with appropriate
capacity.
50115191 B / 11.2010 22
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3.3 Space requirements
EMERGENCY STOP!
The mains socket must always be
accessible for the case of an EMERGENCY
STOP.
Fig. 1: When installing the device, make sure that the

installation and supply connections are freely
accessible.
The control box on the rear panel of the device may
serve as a spacer to adjacent objects. The side
distances given are minimal distances.
To protect the CO2 incubator against contamination,
use a floor stand even if the device is installed near the
floor. The height of the floor stand should not fall
below 200 mm.
Accessibility of the devices:

To ensure the accessibility for care and
maintenance works, keep larger side and
rear distances.
Fig. 1: Device dimensions
50115191 B / 11.2010 23
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3.4 Transport
Fig. 2: For transport, do not lift the device using the

doors or components attached to the device (e.g.
control box on rear panel) as lift points.
Lift points:
Lift the device only using the lift points.
3.5 Stacking
Fig. 3: HERAcell® 150i / 240i is suited for stacking up

to two devices of the same device type. For thermal
separation, an adapter plate [3] can be placed between
the two devices. Fig. 2: Lift points
HERAcell® 150i
• Place the adapter plate with the receptacles [4] at
the bottom onto the stacking elements [5] on top
of the device. Make sure that the adapter plate is
seated safely on the stacking elements.
• Place the device to be stacked with the device
stands [1] onto the stacking elements [2] at the top
of the adapter plate.
HERAcell® 240i
• Place the adapter plate with the receptacle
recesses [8] at the bottom onto the stacking
elements [9] on top of the device. Make sure that
the adapter plate is seated safely on the recesses.
• Place the device to be stacked with the device
stands [6] onto the stacking elements [7] at the top
of the adapter plate.
The devices are secured to one another by their own
weight.
Transporting stacked devices!

The stacking elements are not locking
elements. Therefore, it is not permitted to
transport stacked devices.
Fig. 3: Stacking
50115191 B / 11.2010 24
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Mobile racks Fastening on mobile racks:

If the devices are installed onto mobile racks, make sure that
the rollers [10] are secured with a locking brake during the
operation of the incubators and that the rollers are oriented
toward the front for increased stability.
Condensate formation during the operation of stacked devices:

If stacked devices of type HERAcell® 150i are operated at an
ambient temperature of more than 26 °C, condensate formation
may occur on the upper device while the contra-con
decontamination routine is run for the lower device.
To prevent condensate formation, an adapter plate should be
placed between the two devices for thermal separation.
3.6 Retrofitting/Modifications
Retrofitting options The following components can be retrofitted to the standard version:
HERAcell® 150i
• 3-segment gas-tight screen (the gas-tight screen is installed in place of
the glass door),
• door hinge replacement on outer door and glass door (RH / LH door
opening),
• lockable outer door,
• split inserts,
• infrared (IR) sensor,
• PCB for USB interface.
HERAcell® 240i
• 6-segment gas-tight screen (the gas-tight screen is installed in place of
the glass door),
• door hinge replacement on outer door and glass door,
• lockable outer door,
• split inserts,
• infrared (IR) sensor,
• PCB for USB interface.
Modifications:
Retrofittings and modifications must only be performed by the
Technical Service of Thermo Electron LED.
50115191 B / 11.2010 25
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4 Description of the device
4.1 Front view of HERAcell® 150i
Front view [1] Stacking elements

[2] Plug caps
[3] Glass door
[4] Measuring cell with blower wheel and sensors
[5] Door switch
[6] Oxygen sensor (optional)
[7] Pressure compensation opening with insert
[8] Measuring opening
[9] Outer door
[10] Outer door seal, replaceable
[11] Stand, height-adjustable
[12] Gas humidification (optional)
[13] Water level sensor
[14] Nameplate
[15] Power switch
[16] Support rail
[17] Shelf
[18] Latch, glass door
[19] Support hook for shelf
[20] Access port with plug
[21] Glass door seal, replaceable
[22] iCan™ Touchscreen (Operating panel)
[23] Door handle
50115191 B / 11.2010 26
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Fig. 4: Front view HERAcell 150i
50115191 B / 11.2010 27
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4.2 Rear view HERAcell® 150i
Rear view [1] Access port, Ø 42 mm

[2] Pressure compensation opening
[3] Control box with supply interface for combined gas connection
(optional) CO2 and O2/N2 without gas monitoring
[4] Condensate drain gutter
[5] Diagram: Gas connection CO2 and O2/N2 without gas monitoring
[6] O2/N2 gas cylinder
[7] CO2 gas cylinder
[8] Secondary CO 2 gas supply B with optional gas monitoring
[9] Primary CO2 gas supply A with optional gas monitoring
[10] Secondary O2/N 2 gas supply B with optional gas monitoring
[11] Primary O2/N 2 gas supply A with optional gas monitoring
(optional) CO2 and O 2/N 2 with gas monitoring (optional)
[13] Distributor connection for looping through the CO2 gas supply for
up to three additional devices (independent of device type)
[14] Distributor connection for looping through the O2/N2 gas supply for
up to 3 additional devices (independent of device type)
[15] Diagram: Gas connection CO2 and O2/N2 with optional gas
monitoring
50115191 B / 11.2010 28
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Fig. 5: Rear view HERAcell 150i
50115191 B / 11.2010 29
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Looped-through gas supply for HERAcell® 150i:

(optional) CO2 and O2/N2 with gas monitoring (optional)
[2] Distributor connection for looping through the CO2 gas supply for up
to three additional devices (independent of device type)
[4] Tee joint for fitting the gas pressure hoses together
[5] Gas pressure hose for looping-through the gas supply
monitoring
[7] HERAcell® 150i with looped-through gas supply; therefore device
must only be equipped with combined gas connection (optional) CO2
and O2/N2
50115191 B / 11.2010 30
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Fig. 6: Looped-through gas supply

®
HERAcell 150i
50115191 B / 11.2010 31
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4.3 Front view HERAcell® 240i
Front view [1] Stacking elements

[2] Plug caps
[3] Glass door
[4] Measuring cell with blower wheel and sensors
[5] Door switch
[6] Oxygen sensor (optional)
[7] Receptacle for bottle turning device (optional)
[8] Measuring opening
[9] Plug for bottle turning device (optional)
[10] Outer door
[11] Outer door seal, replaceable
[12] Stand, height-adjustable
[13] Drive roller for bottle turning device (optional)
[14] Gas humidification (optional)
[15] Water level sensor
[16] Insert for bottle turning device (optional)
[17] Nameplate
[18] Cylinders (optional)
[19] Power switch
[20] Support rail
[21] Shelf
[22] Latch, glass door
[23] Support hook for shelf
[24] Pressure compensation opening with insert
[25] Access port with plug
[26] Glass door seal, replaceable
[27] iCan™ Touchscreen (Operating panel)
[28] Door handle
50115191 B / 11.2010 32
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Fig. 7: Front view HERAcell 240i
50115191 B / 11.2010 33
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4.4 Rear view HERAcell® 240i
Rear view [1] Pressure compensation opening

[2] Access port, Ø 42 mm
[3] Condensate drain gutter
(optional) CO2 and O2/N2 without gas monitoring
[5] Diagram: Gas connection CO2 and O2/N2 without gas monitoring
[6] O2/N2 gas cylinder
[7] CO2 gas cylinder
[8] Secondary CO 2 gas supply B with optional gas monitoring
[9] Primary CO2 gas supply A with optional gas monitoring
[10] Secondary O2/N 2 gas supply B with optional gas monitoring
[11] Primary O2/N 2 gas supply A with optional gas monitoring
(optional) CO2 and O 2/N 2 with gas monitoring (optional)
[13] Distributor connection for looping through the CO2 gas supply for
up to three additional devices (independent of device type)
[15] Diagram: Gas connection CO 2 and O 2/N 2 with optional gas
monitoring
50115191 B / 11.2010 34
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Fig. 8: Rear view HERAcell® 240i
50115191 B / 11.2010 35
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Looped-through gas supply for HERAcell® 240i:

(optional) CO2 and O2/N2 with gas monitoring (optional)
[2] Distributor connection for looping through the CO2 gas supply for up
to three additional devices (independent of device type)
[4] Tee joint for fitting the gas pressure hoses together
[5] Gas pressure hose for looping-through the gas supply
monitoring
[7] HERAcell® 240i with looped-through gas supply; therefore device
must only be equipped with combined gas connection (optional) CO2
and O2/N2
50115191 B / 11.2010 36
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Fig. 9: Looped-through gas supply

®
HERAcell 240i
50115191 B / 11.2010 37
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4.5 Safety devices
The device is equipped with the following safety devices:

• A door switch interrupts the CO2/O2/N2 supply and the work space
heating when the glass door is opened.
• The optional gas detector switches the gas supply over to a full gas
cylinder.
• An independent thermal protection protects the samples from harmful
overheating in case of failures.
• A pressure compensation opening ensures pressure compensation in
the device work space.
• The alarm relay circuit uses audible and visual alarms to indicate errors
during operation.
4.6 Work space atmosphere
In the work space of the incubator, the particular physiological ambient

conditions for the preparation and cultivation of cell and tissue cultures are
simulated. The work space atmosphere is determined by the following
factors:
• Temperature
• Relative humidity
• CO2 concentration
• O2 concentration (optional)
Temperature:
Temperature conditions To ensure undisturbed operation, the temperature in the operating room
must be at least 18 °C and the incubation temperature must exceed this
temperature by at least 3 °C.
The heating system controls the incubation temperature from this
temperature threshold up to 55 °C. The principle of air jacket heating and
the additional, separate heating of the outer door and glass door/gas-tight
screen minimize the risk of condensate formation at the side walls, at the
ceiling of the work space, and at the glass door/gas-tight screen.
Relative humidity:
Water quality The heating of the work space promotes the condensation of the water,
thereby ensuring a constant humidity within the work space. For the
running operation, always keep a sufficient quantity of processed water of
the following quality available:
• HERAcell® 240i: quantity 4.5 l,
• HERAcell® 150i: quantity 3.0 l,
Water quality recommendation:

For trouble-free operation of the equipment, fill the water reservoir with
sterilized destilled water or equivalent. The acceptable conductivity should
be within the range of 1 to 20 μS (resistivity within the range of 50 kΩ to
1 MΩ).
50115191 B / 11.2010 38
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Termination of warranty!
Using chlorinated tap water or additives that contain chlorine
will void the manufacture warranty. Similarly, the use of
ultrapure water whose conductivity is out of the range of 1 to
20 μS (and whose resistivity is out of the range of 50 kΩ to
1 MΩ) will void the manufacture warranty.
If you should have any questions, please contact Technical
Support of Thermo Fisher Scientific.
Under normal operating conditions and at the usual incubation

temperature of 37 °C, a constant relative humidity of approx 93% is
achieved in the work space.
If dew formation occurs on the culture containers due to
elevated relative humidity, the humidity in the work space can be adapted
to a lower value by enabling the Low Humidity feature. The relative
humidity in the work space is lowered from approx. 93% to approx. 90%.
The modification requires an extended adaption phase. To ensure that it
effectively prevents
dew formation on culture containers, it must be used as a permanent
setting.
CO2 supply:
CO2-Work space atmosphere control To ensure the growth conditions for the cell and tissue cultures, the work
space is supplied with CO2.
The pH of the bicarbonate-buffered culture media largely depends on the
CO2 content of the work space atmosphere.
The CO2 content of the work space atmosphere can be controlled within a
range of 0 to 20%.
The supplied CO2 must have one of the following quality characteristics:
• Purity 99.5% min.,
• medical gas quality.
O2 supply:
O2-Work space atmosphere control If the CO2 incubator is to be operated with more than 21% oxygen, the
work space is supplied with oxygen. The O2 content of the work space
atmosphere can be controlled within a range of 0 to 21%.
N2 supply:
N2-Work space atmosphere control If the oxygen content during operation is to be lowered to less than 21%
(air oxygen content), the work space is supplied with nitrogen. The O2
content of the work space atmosphere can be controlled depending on the
configuration of the sensor within a range of 1% to 21% and 5% to 90%.
50115191 B / 11.2010 39
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4.7 Door switch
A door switch [1] is installed at the upper edge of the

work space opening. If the door switch is activated by
opening the glass door, the gas supply and the heating
of the work space are interrupted and the display
shows a corresponding message.
If the door remains open for more than 30 seconds, a
short audible alarm sounds. If the door remains open
for more than 10 minutes, an audible alarm sounds
and the alarm relay responds.
The outer door can only be closed after the glass door
has been latched properly.
Versions with gas-tight screen:

For devices with the optional gas-tight
screen, the door switch function described
Fig. 10: Door switch
above is activated when the outer door is
opened.
50115191 B / 11.2010 40
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4.8 Sensor system
The blower wheel and two sensor modules are

integral to the baseplate [1] of the measuring cell:
• Sensor [2] for the acquisition of the work space
temperature and the thermal protection,
• CO2 sensor [3] for the acquisition of the CO2
content in the work space atmosphere.
The O2 sensor (optional) [4] for the acquisition of the

O2 content in the work space atmosphere is installed
at various locations next to the measuring cell:
• HERAcell® 150i in the sidewall in the upper area [5],
• HERAcell® 240i in the ceiling of the work space [4].
The sensor for the acquisition of the work space

temperature as well as the CO2 sensor and the O2
sensor are integral to the control system of the device.
Their measured values are compared to the selected
set values. Based on this data, the control system
controls heating and CO2/O2/N2 supply.
The blower intermixes the supplied gases and ensures
an even temperature distribution within the work
space.
The thermal protection has been preprogrammed at

the factory and cannot be changed. It protects the
stored cultures from overheating.
If the set temperature is exceeded by more than 1°C,
the thermal protection responds and the work space
temperature is automatically reduced to the selected Fig. 11: Temperature, CO2,and O2 sensors
set value so that the incubation process can be
continued even in case of a failure. Any response of
the thermal protection will simultaneously trigger a
visual alarm.
If the thermal protection is enabled:
• an error message is issued,
• the alarm relay responds.
If the error message is accepted, the display shows the
Overtemperature icon to indicate the response of the
thermal protection, and the temperature display is
highlighted in red.
50115191 B / 11.2010 41
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4.9 Supply interface
All supply connections are installed in the supply

interface (control box [1]) in the rear of the device.
Gas connection:
The gas supply lines between the device and the gas
supply system are connected using the supplied
connecting hoses. O2/N2 is supplied to the device
through connecting sleeve [2]. CO2 is connected to
sleeve [3].
All process gases must be supplied to the device at a
fixed pressure that has been preset within a range of
0.8 to 1.0 bar and must remain unchanged.
Before the gases are fed into the work space, they flow
through a gas inlet filter with a separation rate of 99.97
% for a particle size of 0.3 μm (HEPA filter quality).
The illustration shows the optional combined gas
connection without the optional gas monitoring
system.
Label:
The label [4] contains information about gas supply,
an alarm contact terminal legend, and notes about the
electrical fusing of the device.
RS 232 interface:
Via the RS 232 interface [5], the incubator can be
connected to the serial interface of a PC. This
connection allows the computer-aided acquisition and
documentation of the major operating parameters
(temperature, CO2/O2/N2 concentration, error codes,
etc.).
USB interface (optional):

As an alternative to the RS 232 interface [5], the
incubator can be connected to a PC via the optional
USB interface [6]. This connection - USB 1.1 /
USB 2.0 full speed compatible - allows the computer-
aided acquisition and documentation of the major
operating parameters (temperature, CO2/O2/N2
concentration, error codes, etc.).
Fig. 12: Supply interface
50115191 B / 11.2010 42
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Alarm contact:
External alarm system Fig. 12: The device can be connected to an on-site, external alarm system
(e.g. telephone system, building monitoring system, visual or audible
alarm system).
For this purpose, a potential-free alarm contact [7] is preinstalled in the
device.
Alarm contact:
The alarm contact responds for all errors reported by the
control loops (see chap. 7.13‚ pg. 95).
Power supply connection:

Fig. 12: The connection to the power supply system is
established by connecting a cable with plug for IEC connector
[9] to the socket [8]. The receptacle for the two device fuses is
integral to the socket.
50115191 B / 11.2010 43
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4.10 Work space components
The work space of the incubator has only a minimum

of surface, thereby supporting both the prevention of
contamination and easy, effective decontamination.
4.10.1 Interior container

All components of the work space are made of
stainless steel and have a high-gloss polished,
absolutely smooth and easy-to-clean surface. Any
embossings have a large radius.
As an option, the interior container, the shelf system,
and the blower wheel with its cover can be made of
copper material.
Materials of the interior container

The standard version is equipped as follows:
• Interior container made from stainless steel.
Materials with increased contamination protection
are optionally available:
• Interior container made from stainless steel, with
transparent iONGURAD™ silver ion coating,
• Interior container made from copper, combined
with the following components also made from
copper material: Shelf system and blower wheel
with protective grid (cover).
Oxidation of copper components:

When exposed to heat and humid air, the
copper material of the interior container
will oxidize. This results in a discoloration
of the copper components during the test
run for checking the device functions.
Do not remove the oxide layer during
routine cleaning as the antimicrobial effect
of the copper material is based on it.
The components of the shelf system can be removed

easily so that only the easily treatable, surface-
reduced interior container [1] remains in the work
space for cleaning and manual disinfection works.
4.10.2 Optional split gas-tight screens

Equipment versions:
• HERAcell® 150i with 3-segment gas-tight screen [2],
• HERAcell® 240i with 6-segment gas-tight screen [3].
Fig. 13: Work space components
50115191 B / 11.2010 44
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For devices that are equipped with the optional gas-

tight
screen, the contamination hazard is considerably
lower and the recovery times of the incubation
parameters are shorter for:
• work space temperature,
• CO2 concentration,
• O2/N2 concentration,
• relative humidity.
Advantage of the gas-tight screen: The cross sections
of the openings are smaller when samples are
accessed.
4.10.3 Water reservoir

The water reservoir [1] is integral to the interior
container floor and inclines toward the rear. The water
level is monitored by a water level sensor [2] that
issues an alarm message at the display and an audible
signal when the water falls below the minimal level.
The embossings [3] in the water tray are used as
indicators for the maximal level.
4.10.4 Gas humidification (optional, only for O 2

control)
A hose [5] connects the gas humidification [6] to the
device-integral oxygen or nitrogen supply line [4]. The
inflowing oxygen or nitrogen is fed to the heated
water. This ensures humidification of the gases as
they enter the work space and prevents an undesired
drop of the work space humidity.
4.10.5 Heating system

An air jacket heating is used for heating the work
space. The arrangement of the heating elements
ensures that condensate formation above the water
reservoir is prevented as far as
possible.
The outer door of the device is also heated. The heat
radiated onto the interior glass door/gas-tight screen
prevents condensate formation. The work space of the
device always remains visible, despite high humidity.
Fig. 14: Water reservoir
50115191 B / 11.2010 45
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4.10.6 Rear panel openings

A pressure compensation opening with insert in the
rear panel of the device allows a compensation
between the pressures in the work space and in the
operating room.
A sealable access port allows cables, hoses or
additional sensors to be routed into the work space of
the device.
HERAcell® 150i:
– access port, Ø 42 mm [1]
– pressure compensation opening [2]
HERAcell® 240i:
– access port, Ø 42 mm [3]
– pressure compensation openingt [4]
Operating conditions:
When accessories are to be operated in the
work space of the CO2 incubator, the
ambient condition requirements must be
observed (see table below). The energy
introduced into the work space affects the
beginning of the temperature control
range. When additional heating sources are
introduced into the work space,
condensation (e.g. at the glass door) may
occur.
Introduced Control range of the temperature

energy
Example:
General RT* = 21° C
0W RT + 3° C 24° C
5W RT + 6,5° C 27,5° C
10 W RT + 9,5° C 30,5° C
15 W RT + 13° C 34° C
Fig. 15: Rear panel openings
20 W RT + 16° C 37° C
*RT = Ambient temperature
50115191 B / 11.2010 46
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4.10.7 Shelf system

Fig. 16: The support rails [1] of the shelf system are
perforated every 42 mm so that the support hooks [8]
can be inserted variably for any culture container size
required. The shelves [2] have an integrated tilt
protection and withdrawal stop. For details of the shelf
system, see Section „Start-up“.
4.10.8 Bottle turning device for HERAcell® 240i

(optional)
Fig. 16: HERAcell® 240i may be equipped with up to
four separate bottle turning devices. Each unit
consists of a roller insert [6] with a drive roller [4] and
four idle rollers [7] that are also rotated due to the
transmission function of the inserted cylinders [5].
Each unit is controlled separately as the drive roller is
connected to the device control unit by a connector [3]
at the right side of the device.
This modular layout also allows the operation of only
one bottle turning device in combination with several
shelves.
The arrangement of the idle rollers in the roller insert
frame depends on the configuration of the cylinders
used. The drive roller is always inserted into the right
roller guide. The position and the number of the idle
rollers in the frame then depend on the cylinder
diameter and on the number of cylinders. The figure
shows three examples of different idle roller positions
in dependence of the cylinder diameter.
The arrangement of the roller inserts in the work space
is designed alphabetically as a, b, c, and d with a being
the lowest and d being the uppermost roller insert.
The connections of the drive rollers are assigned
correspondingly to the device control unit. The drive
roller of the switching insert a must only be connected
to the lower receptacle a of the corresponding control
unit.
Connection assignment:
If the drive roller is not properly connected
to the control unit, the parameters cannot Fig. 16: Bottle turning device
be set correctly on the operating panel of
the device.
50115191 B / 11.2010 47
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Receptacles:
To prevent corrosion due to moisture in the
work space, unassigned receptacles of the
bottle turning device must always be
plugged with the protective cap.
The rotating speed of the bottle turning devices is set

depending on two parameters:
• Diameter (of the cylinders used),
• drive roller set value.
The value is determined using a diagram (see chap.
5.5‚ pg. 52).
4.10.9 Water pump

Fig. 17: The water pump serves for suction cleaning of
the remainig water in the water reservoir. The water
drain works through gravity.
1. Place the water pump [1] on lowest shelf [5].
2. Feed the inlet hose [4] through a whole [7] of the
lowest the shelf to the water reservoir [8].
3. Prepare a bucket [2].
4. Suck the water, therefore pump the water pump
using the hand grip [6] about four times until
water flows in the outlet hose.
5. Let the water run into the bucket [2] through
gravity.
6. Wipe out the rests of the water at the floor plate of
the water reservoir.
Fig. 17: Water pump
50115191 B / 11.2010 48
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5 Start-up
5.1 Let the device adapt to the ambient conditions
Let the device adapt to the ambient conditions!

The device must adapt to ambient conditions prior to start-up.
• Set up the device in the operating room and let it adapt to
the expected operating room temperature
• for a period of approx. 2 hours prior to switching it on.
• Open the device doors.
5.2 Preparing the work space
7. Upon delivery, the CO2 incubator is not in a sterile state. Before the
initial start-up, the device must be decontaminated.
Before the decontamination is performed, the following work space
components must be cleaned:
• Support rails,
• support hooks,
• shelves,
• bottle turning assembly (optional),
• gas humidification (optional),
• work space surfaces,
• work space seals and gaskets,
• glass door/gas-tight screen.
Decontamination:
For details about the cleaning and disinfection of the device,
(see chap. 9‚ pg. 101).
50115191 B / 11.2010 49
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5 Start-up
5.3 Installing the shelf system
Tools are not required for the installation of the shelf

system. The support rails are secured using spring
pressure. After the support hooks have been inserted
into the rail, the shelves are pushed onto the support
hooks.
5.3.1 Installation/removal of the support rail

The support rails are held at the sides by the
embossings [2] and [5] and secured by the
embossings [1] and [6]. The support rails marked with
the diamond shape are inserted at the rear panel of the
device with the locksprings [3] facing upward.
1. Position the support rail [4] onto the lower
embossing [6] and tilt it toward the work space
side wall so that the rail is positioned over the two
embossings [5] and [2].
2. Clamp the lockspring [3] behind the upper
embossing [1].
3. To remove the support rails, pull the lockspring
tab down out of the embossing and remove the
rail.
Fig. 18: Support rail installation/removal
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5 Start-up
5.3.2 Installing the shelf supports:

1. Fig. 19: Insert the shelf supports [3] into the
perforations [1] of the support rail with the bar
facing down.
2. Make sure that the two vertical elements [2] of the
shelf support are flush with the support rail.
Installing the shelves:

1. Push the shelf [4] onto the shelf supports with the
tilt protection [5] facing the rear panel of the
device. The tilt protection [5] is also used as a
guide for the shelf.
2. Slightly raise the shelf so that the withdrawal stop
[6] can be routed over the shelf supports.
3. Make sure that the shelf support is positioned in
the two tilt protections in a way that allows it to
move freely.
5.3.3 Levelling the device

1. Position a bubble level onto the center shelf or
onto the roller holder.
2. Rotate the adjustable device stands using the
supplied 24 mm wrench until the shelf is
positioned horizontally in all directions. Perform
the adjustment of the device stands from left to Fig. 19: Shelf support installation
right and from rear to front.
5.4 Installing the gas humidification

(optional, only for O2/N2-control)
Fig. 20: The gas humidification [1] is installed to the

water tray parallel to the rear panel of the device. The
position to the right side wall is predetermined by the
hose length.
1. Install hose [2] to the sleeve of the gas
humidification and then to the sleeve [3] of the
device-integral oxygen or nitrogen supply line.
2. Place gas humidification immediately to the rear
panel of the device and align it parallel to the rear
panel.
Fig. 20: Installing gas humidification
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5 Start-up
5.5 Installing the optional bottle turning

devices HERAcell® 240i
1. Align the roller insert so that the roller holder [8]

and the crossmember [7] are flush and facing the
work space opening.
2. Slide the roller insert with both guide rails [5] onto
the shelf supports.
3. Slightly raise the roller insert so that the
withdrawal stop can be routed over the shelf
support.
4. Insert the drive roller [6] into the right receptacle
and connect the cable [4] to the corresponding
control unit a.
5. Insert the four idle rollers [3] the into receptacles
and position the rollers in the receptacles in
accordance with the bottle diameter.
6. Place the bottles onto the rollers. To ensure that Fig. 21: Installing the bottle turning devices
the bottles will not be shifted against each other

by the rotation, the bottoms of the bottles should
contact the stop disks [2] of the rollers and the
cylinder necks [1] should contact the rubber stop
rings [8]. The stop ring can be positioned
accordingly on the roller.
Set speed of the bottle turning device:

7. The rotating speed of the bottle turning devices is
set depending on two parameters:
– Diameter (of the bottles used),
– drive roller set value.
Use of diagram:
8. The diagram shows the common bottle diameters
as different graphs (ascending straight).
– Set the desired speed at the Y axis. Read the
corresponding set value (in %) from the X axis
at the point of intersection of the Y value with
the specific graph.
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5 Start-up
Fig. 22: Bottle turning speed
Example:
– Bottle diameter: 56 mm
– Desired speed: 3.5 rpm
– Determined set value: 48%
In the dialog box SPEED BOTTLE TURN. DEVICE, the value 48% is set (see
chap. 7.11.3‚ pg. 85).
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5 Start-up
5.6 Installing the optional center strut

HERAcell® 240i for split inserts
If HERAcell® 240i is equipped with the optional 6-

segment insert, two center struts [1] with perforations
at each side must be installed.
In this case the shelf support rails must be installed to
the support rails at the sides and at the left and right
side of the strut.
The support rails marked with the diamond shape are
installed at the rear panel of the device.
1. A pressure spring keeps the upper rectangular
guide [3] of the center strut under tension. Insert
this guide into the receptacle [2] in the work space
ceiling und push it slightly upward so that it is
pushed into the center strut.
2. Then, position the lower rod guide [5] of the
center strut into the hole [6] in the work space
floor and remove spring tension.
3. The center strut is stabilized by the spring
pressure.
Configuration with split inserts:

For versions with split inserts, bottle
turning devices cannot be installed.
Fig. 23: Center strut installation
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5 Start-up
5.7 Gas connection
Gas quality:
The gases must have one of the following
qualities:
• Purity 99.5 % min,
• medical gas quality.
Overpressure!
The operating pressure of the gas applied
to the device must not exceed 1 bar. If the
gas is supplied at a higher pressure, the
valves integral to the device may not close
correctly and the gas supply control may
be impaired.
Set the gas supply to a range between 0.8
bar min. and 1.0 bar max. and make sure
that this pressure setting cannot be
changed!
5.7.1 Installing gas pressure hoses

The gas supply from the gas supply system to the
device is achieved using the supplied flexible gas
Fig. 24: Installing gas pressure hoses
pressure hoses:
1. Connect the gas pressure hose [2] to the sleeve of
the gas supply system.
2. Remove the protective cap [3] from the sterile
filter.
3. Slide the hose clamp [1] onto the gas pressure
hose and connect hose to the sleeve [4] of the
sterile
filter [5].
4. Secure the gas pressure hose to the sleeve of the
sterile filter using the hose clamp.
Pressure compensation opening:

To ensure permanent pressure
compensation, the pressure compensation
opening must not be connected to an
exhaust air system. The pipe of the
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5 Start-up
pressure compensation opening must not

be extended or redirected.
5.7.2 Installing devices without the optional gas

monitoring system
device is established using the supplied flexible gas
pressure hoses (see chap. 5.7.1‚ pg. 55):
CO2 connection:
• For a device with CO2 connection, connect the gas
supply to the sterile filter [2] in accordance with the
connection diagram [1].
Combined CO2 and O2/N2 connection (optional):

For a combined CO2/O2/N2 connection, proceed as
follows in accordance with the connection diagram
[3]:
• Connect the O2/N2 supply line to the upper gas
inlet filter [4],
• connect the CO2 supply line to the lower gas inlet
filter [5].
Manual gas monitoring:

On devices without the optional gas
monitoring system,
there is no automatic gas monitoring.
Therefore, the filling level of the gas bottles
must be checked every day.
Fig. 25: Gas connection without gas monitoring system
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5 Start-up
5.7.3 CO 2 connection with gas monitoring

system (optional)
device is established by the supplied flexible gas
pressure hoses.
Fig. 26: Devices with CO2 connections that are
equipped with the optional gas monitoring system are
connected according to the connection diagram [7].
CO2 connection:
For a device that is operated with process gas CO2
and equipped with the optional gas monitoring
system, connect the gas supply lines as follows:
• Distributor connection [5] for the CO2 gas supply
for up to three additional devices. For looping the
gas supply through, interconnect the devices with
a gas pressure hose.
• Connect the gas container B to the upper
connection [2] of the gas monitoring system,
• connect the gas container A to the lower
connection [3] of the gas monitoring system. Fig. 26: Gas connection with gas monitoring system (optional)
• The outlet of the gas monitoring system [4] is
connected at the factory to the gas inlet filter [5] by
a short gas pressure hose [6].
Looping the gas supply through:

By looping the gas supply through, up to 4
devices can be supplied by a CO2 gas
supply.
5.7.4 Combined CO2/O2/N2 connection with gas

monitoring system (optional)
device is established by the supplied flexible gas
pressure hoses (see chap. 5.6.1‚ pg. 47).
Fig. 27: Devices with the combined CO2/O2/N2
connection that are equipped with the optional gas
monitoring system are connected according to
connection diagram [13] and have the following gas
connections:
O2/N2 supply:
• Distributor connection [1] for the O2/N2 gas supply
• Connect the gas container B [2] to the upper
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5 Start-up
connection of the gas monitoring system,

• connect the gas container A [3] to the lower
connection of the gas monitoring system,
connected at the factory to the gas inlet filter [9] by
a short gas pressure hose [11].
CO2 supply:
• Distributor connection [5] for the CO2 gas supply
• Connect the gas container B to the upper
connection [6] of the gas monitoring system,
• connect the gas container A to the lower
connection [7] of the gas monitoring system.
connected at the factory to the gas inlet filter [10]
by a short gas pressure hose [12].
Looping the gas supply through:

By looping the gas supply through, up to 4
devices (see annex at the end of this
section) cyn be supplied by a CO2 gas
supply or O2/N2 gas supply.
Fig. 27: Combined connection with

gas monitoring system (optional)
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5 Start-up
5.8 Power supply connection
Electric shock!
Contact with current-carrying components
may cause a lethal electric shock.
Before connecting the device to the power
supply, check plug and power supply cable
for damage.
Do not use damaged components when
connecting the device to the power supply!
The device must be connected only to a correctly

installed and grounded power supply source:
• Fusing T 10 A
• Circuit breaker B 16
Connection to the power supply system:

1. Before connecting the device to the power
supply, check to see if the voltage of the power
supply corresponds with the specifications on the
nameplate on the front of the device. If the ratings
given for voltage (V) and current (A) are not
correct, the device must not be connected to the
power supply.
2. Connect the IEC connector [2] to the socket [1] of Fig. 28: Combined gas connection with gas monitoring system
the control box of the device.
3. Connect the grounding plug [3] of the power
supply cable to a correctly grounded and fused
socket.
4. Make sure the power supply cable is not
subjected to tensile or compressive force.
Fig. 28: Power supply connection
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5 Start-up
5.9 Connecting the RS 232 interface
Data communication via RS 232 The RS 232 data communication interface has been designed for a cable
connection with 9-pin connector and a contact assignment of 1:1.
Data exchange is accomplished by a predefined structure of command
sequences (see annex at the end of this section).
Data communication diagram:

The command sequence syntax as well as the RS 232 interface
data communication diagram are described in the annex at the
end of this section.
Connecting the devices:

1. Turn the PC off.
2. Connect the serial interface cable connector (not included in the
standard equipment) to the socket at the device supply interface at the
rear of the device.
3. Connect the second connector to an unassigned serial port COM 1 /
COM 2 etc. at the PC.
4. Turn the PC on.
5.10 Connecting the USB interface (optional)
Data communication via USB As an alternative to data communication via RS 232 interface, the devices
can be equipped with the optional USB interface. The USB interface
complies with Standard USB 1.1 and is compatible with Standard USB 2.0
(full speed).
The interface stepping rate can be changed within the defined baud rates
(9,600, 19,200, 38,400, 57,600 baud).
Data exchange is accomplished via a defined command sequence
structure (frames). The command sequences correspond with the RS 232
interface installation diagram (see annex at the end of this section).
Using the USB interface for data communication between a PC and the
device a driver must be installed first (see annex data communication).
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5 Start-up
5.11 Connecting the alarm contact
Skilled work:
Thermo Scientific warrants the operational safety and the
operativeness of the device only if installation and repairs are
performed properly.
The connection of the device to an external alarm system must
only be carried out by adequately trained and authorized
expert electrical/telecommunication personnel!
Function:
When system errors and failures occur in the temperature or gas control
circuits, an alarm message is issued to the connected alarm/monitoring
system. The potential-free contact (1 changeover contact) has been
designed for the following circuits:
Alarm relay:
Circuit Voltage External fusing

Circuits with system voltage max. 250 V ~ max. 6 A
"SELV circuits 25 V ~ max. 2 A
(cf. VDE 0100, Part 410)" 60 V = max. 1 A
SELV E – circuits 50 V ~ max. 1 A
(cf. VDE 0100, Part 410)" 120 V = max. 0,5 A
Contact Contact
Operating states
4-1 4-3
Operating state power failure "off" X O
Operating state power failure "on" O X
Failure: power failure "off" O X
Failure: power failure "on" X O
X: Contact closed / O: Contact open
Switching structure:
The alarm relay switches for all failures reported by the control
loops (see chap. 7.13‚ pg. 95).
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5 Start-up
Connection example:
The connector [5] for the connecting cable is included
in the standard equipment. The values for the
operating voltage of the external circuits and of the
fusing of the alarm system are given in the table on the
previous page.
1. Connect the individual conductors [1] to [4] of the
connecting cable as shown in the wiring diagram.
2. Connect the connector [5] of the alarm system
connecting cable to the interface [6] at the control
box at the rear panel of the device.
Fig. 29: Connection example
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6 Operation
6.1 Preparing the device
The device must only be released for operation after all major measures for
the start-up have been taken (see Section 5, page 45).
Device check:
Device check Prior to starting operation, the following device components must be
checked for their correct function
• Gas hoses must be seated tight on the connecting filter and must be
secured using a hose clamp.
• The access port must be capped.
• The pressure compensation opening must be permeable, its insert
must be installed in the work space.
• The glass door/gas-tight screen seal must not be damaged.
• The glass door measurement opening must be capped.
• The shelf system components must be installed safely.
• The gas humidification (optional) must be connected to the device-
integral gas supply and aligned parallel to the rear panel.
• The drive rollers of the bottle turning device (optional) must be properly
connected to the corresponding control unit.
• The idle rollers of the bottle turning device (optional) must be
positioned in accordance with the cylinder diameters.
Decontaminating the device work space:

Decontamination Run the contra-con decontamination routine (see chap. 9.4‚ pg. 104) or
decontaminate the work space according to the hygiene regulations set
forth by the operator.
Hygiene regulations:
Prior to any operation, the user must clean and disinfect the
work space in accordance with the hygiene regulations set
forth by the operator to protect the cultures.
The “Principles of good microbiological proceedings” at the
annex of these instructions can be used as safety information
for personnel working with the device.
Water supply:
A sufficient quantity of treated water of the following quality
must always be available for the running operation:
• distilled, fully demineralized, and
• autoclaved, sterile.
When the filling level drops below a minimum value, it must be
possible to top up water during the work process.
Capacities:
• HERAcell® 150i: 3.0 l
• HERAcell® 240i: 4.5 l
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6 Operation
6.2 Starting operation
1. Fill the water tray with a sufficient quantity of processed water. Do not
exceed the upper level mark.
2. Make sure that the CO2/O2/N2 supply system valves are open.
3. Turn the device on using the power switch.
4. Set the set values for temperature and CO2 / O2 content on the iCan™
touchscreen.
5. Start contra-con.
Starting the device:

Starting with auto-start 6. Start the device with the auto-start routine (see chap. 7.9.1‚ pg. 75).
7. The progress indicator auto-start appears on the display, and the
automatic start routine is run.
8. The temperature control adjusts the temperature to the selected set
value, humidity rises.
9. When temperature and relative humidity are constant, the automatic
adjustment of the CO2 / O2 measuring system is performed
10. The CO2 / O2 control supplies the set amount of CO2 / O2.
11. If the auto-start routine has been completed, the progress indicator is
hidden, and the main menu is displayed. The device is operational.
Loading the device:

Loading the work space 12. Load the work space with cultures or place bottles onto the bottle
turning device rollers.
Duration of the auto-start routine:

When the device is cold and when the ambient temperature is
low, the auto-start routine may take up to 10 hours.
Loading:
To ensure sufficient air circulation and even heating of the
samples, the loading surface within the work space should be
used up to 70% max. Voluminous objects in the work space
that dissipate heat may impair heat distribution.
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7 Handling and control (iCan™ touchscreen controller)
7.1 Power switch
Fig. 30: Depending on which side the door hinges are

installed, the power switch [1] is integral to the front
cover [2] of one of the front device stands.
• To turn the device on: Press the power switch [1];
the switch illumination comes on.
– After a short audible signal and a short dark
phase, the touchscreen display appears.
– The control loop sensors pass the heat-up
phase (see chap. 7.4‚ pg. 68)
• To turn the device off: Press the power switch; the
switch illumination goes off
7.2 Operating panel and operating

structure
Fig. 31: The control panel works as a touchscreen Fig. 30: Power switch
(iCan™ touchscreen) and can be controlled
performing light pressure on the pressure-sensitive
areas of the screen using a finger or a blunt pin:
• Temperature display [1],
• CO2 display [3],
• O2 display [5] (optional).
• contra-con key [2]
• menu key [4]
• auto-start key [6],
Extended pressure-sensitive area:

To accept a failure message, the entire
touchscreen can be used as a pressure-
sensitive area.
.
Fig. 31: Pressure-sensitive areas
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Versions without O2/N2 control:

Fig. 32: Function keys and value displays of the
operating panel for a device version without O2/N2 gas
supply:
[1] Type designation of the device
[2] Temperature display
[3] CO2 display
[4] Key for starting the contra-con
decontamination routine
[5] Key for opening the menu navigation
[6] Key for starting the auto-start routine
[7] Actual value CO2
[8] Set value CO2
[9] Temperature actual value
[10] Temperature set value
[11] Current date and time
Version with combined CO2/O2/N2 control

(optional):
Fig. 33: Function keys and value displays of the Fig. 32: iCan™ touchscreen withoutO2/N2gas supply
operating panel of a device version with combined
CO2/O2/N2 gas supply:
[1] Type designation of the device
[2] Temperature display
[3] CO2 display
[4] CO2 set value
[5] CO2 actual value
[6] Key for starting the contra-con
decontamination routine
[7] Key for opening the menu navigation
[8] Key for starting the auto-start routine
[9] O2 display
[10] O2 actual value
[11] O2 set value
[12] Temperature actual value
[13] Temperature set value
[14] Current date and time
Structure of the operating levels:

Fig. 34: Operation is divided into three levels:
• A: Direct access to the control loop settings:
Temperature, CO2, O2 set value and bottle turing
device (optional), Fig. 33: iCan™ touchscreen with combined gas connection
• B: Start of the device routines contra-con or auto-
start,
• C: Navigation through the submenus for device
configuration.
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Fig. 34: Menu structure overview
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7.3 Factory presettings of the iCan™

touchscreen controls
Upon delivery of the device, the following set values

have been preset:
• Temperature: 37 °C
• CO2 content: 0.0%
• O2 content (optional): 21.0%
• Optional bottle turningdevice speed a, b, c, and
d: 0%.
CO2/O2 control:
Since the CO2 concentration of the air is
nearly 0%, the CO2 control and the control
Fig. 35: Heat-up phase display
loop error monitoring system are disabled
at a set value of 0%.
Since the O2 concentration of the air is
nearly 21%, the O2 control and the control
7.4 Heat-up phase of the control loop

sensors
When the device has been switched on, the control

loop sensors pass through a heat-up phase of varying
duration during the start process:
Temperature control loop 10 s

CO2 control loop 10 s
CO2 control loop with IR measuring cell 3 min
O2 control loop 5 min
The start process is indicated by an audible signal.

During the heat-up phase, the displays show dots (...)
instead of values:
• Temperature display [2],
• CO2 display [1] and
• O2 display [3]
After the heat-up phase has been completed, the
control loop actual values are indicated.
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Exposure to CO2 gas:

During the 5-minute heat-up phase of the O2 control loop, the
work space is not exposed to CO2 gas and the CO2 control and
is disabled.
7.5 Event actions for settings
Key response:
Pressing a key can increase or reduce a value gradually:
• When the – key [4] or the + key [6] is kept depressed, the system
switches to quick run,
• after the key has been kept depressed for more than 3 seconds, the
quick run speed increases.
Saving the settings:

To save changed values, press the Enter key.
Resetting the settings:

Unless a user action (contact with the pressure-sensitive areas
and keys) occurs within 30 seconds, the system automatically
exits the menu and the most recently confirmed setting
defaults.
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7.6 Setting the temperature set value
1. Press the TEMPERATURE DISPLAY key [1].

> The Temperature menu is displayed.
2. To exit the Temperature menu:

> Press the END key [2].
3. To set the temperature set value:

> Press the SET VALUE key [3].
To increase the set value:

> Press the + key [6].
To reduce the set value:

> Press the - key [4].
4. To accept and save the set value:

> Press the ENTER key [5].
> The system returns to the main menu. The
temperature displays shows the actual value
currently measured in the work space.
Fig. 36: Setting the temperature set value
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7.7 Setting the CO2 set value
1. Press the CO2 DISPLAY key [1].

> The CO2 menu is displayed.
2. To exit the CO2 menu:

3. To set the CO2 set value:




> The system returns to the main menu. The CO2
display shows the actual value currently
measured in the work space.
Deactivating the CO2 control loop:

To deactivate the CO2 control, the set value
is set to 0%.
If the control loop is deactivated, error
monitoring is enabled as well.
Gas monitoring:
The switching state of the CO2 control loop
does not affect the function of the optional
gas monitoring system. The gas monitoring
system remains active even when the CO2
control is switched off.
For set values of 21% O2, the O2 control is
switched off and error monitoring is
disabled as well.
The control loop error monitoring system
(optional) is enabled.
Fig. 37: Setting the CO2 set value
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7.8 Setting the O2 set value
This setting is possible only on versions with the

optional O2/N2 control.
1. Press the O2 DISPLAY key [1].
> The O2 menu is displayed.
2. To exit the O2 menu:

3. To set the O2 set value:




> The system returns to the main menu. The O2
displays shows the actual value currently
measured in the work space.
Factory settings:
Depending on the type of the O2 sensor,
one of the two O2 control ranges has been
preset at the factory:
Control range I: 1% to 21%
Control range II: 5% to 90%
Usage of the process gases:

For set values below 21% O2, the device
must be connected to a nitrogen supply
system.
For set values above 21% O2, the device
must be connected to an oxygen supply
system.
Since the O2 concentration of the air is
nearly 21%, the O2 control and the control
The control loop error monitoring system
(optional) is enabled. Fig. 38: Setting the O2 set value
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7.9 Auto-start routine
The auto-start function is an automated routine for the start and the
subsequent adjustment of the CO2 measuring system. After the start, the
device control adjusts the temperature to the set value while humidity is
generated. When temperature and relative humidity have reached constant
values, the CO2 measuring system is automatically adjusted to these
values, and the work space is supplied with the preset quantity of CO2.
Application of the auto-start routine:

To ensure that the specified accuracy of the CO2 measuring
system is maintained, the device should always be started
using the auto-start routine if
• a difference of more than 1 °C is entered upon setting the
temperature set value,
• the Low Humidity function is enabled/disabled,
• the device is restarted after an extended interruption of
operation.
The auto-start routine should be run at least every three
months on the occasion of cleaning and maintenance works.
Duration of the routine:

Running the routine usually takes 5 to 7 hours. At low room
temperatures and when the device is cold, it may take up to 10
hours until the auto-start routine has been completed. If the
glass door is opened or if the power supply of the device is
interrupted while the routine is running, the routine is
interrupted and rerun after the glass door has been closed or
the power supply has been reestablished.
Start conditions for the auto-start routine:

Prior to running the auto-start routine, set the CO2 and O2 set
values to the desired values and make sure that the
atmosphere in the work space only consists of ambient air.
The floorpan of the device must be filled with a sufficient
quantity of water.
Conditions that prevent the start of the auto-start routine:

The auto-start routine cannot be started if one of the following
failure conditions exists.
Temperature control loop:
• Sensor breakage,
• actual value above set value (excessive deviation),
• actual value below set value (excessive deviation),
50115191 B / 11.2010 73
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• set value not plausible,

• calibration values too high or too low,
• sensor communication failure,
• sensor parameter not plausible,
• no communication with LM 75.
Control loop for CO2 gas supply:

• No communication with sensor.
In this case, the auto-start key is dimmed and its function is not
available.
Faulty cancellation of the auto-start routine:

The auto-start is cancelled if:
• an error is detected in the temperature control loop,
• an error is detected in the CO2 control loop,
• the water filling level is insufficient.
auto-start-Dry execution of auto-start routine:

If the auto-start routine is to be run dry (without water in the
floorpan of the work space), the water level sensor must be
disabled prior to its start (see chap. 7.11.3‚ pg. 85).
50115191 B / 11.2010 74
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7.9.1 Activating auto-start

Preparations for the start:
1. Make sure that the CO2-/O2-/N2 gas supply system
valves are open.
2. Fill the floorpan of the work space with a sufficient

quantity of water. The filling level must not
exceed the upper filling level mark.
3. Set the set values for temperature, CO2 and O2 on

the touchscreen.
auto-start routine activation:

1. Press the auto-start key [2].
> The auto-start-instruction menu [1] is
displayed.
2. To exit the auto-start-instruction menu and

cancel auto-start:
3. Activate the auto-start routine:

> Press the START key [3].
4. To air the work space, open both device doors.
5. When the audible alarm sounds after 30 seconds,

close both device doors.
> The trend status indicator [5] is displayed.
6. During the progress, the status indicator displays

the following information:
– Temperature,
– start time.
Cancellation:
The auto-start routine can be cancelled at
any time!
Press the Stop key [6].
Automatic restart:
The auto-start routine is restarted
automatically if the routine is cancelled due
to one of the following events:
• The glass door is opened, Fig. 39: Activating the auto-start routine
50115191 B / 11.2010 75
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• the outer door is opened on a device

with optional gas-tight screen,
• the power supply is interrupted.
7.9.2 Interrupting the auto-start routine

If the STOP key in the status display is depressed (see
above), the auto-start routine is interrupted and the
auto-start stop dialog box is displayed for a safety
scan. The routine can now be permanently cancelled
or resumed.
1. To resume the auto-start routine:

> Press the BACK key [1].
> The system returns to the status display, and
the auto-start routine is resumed.
2. To cancel the auto-start routine:

> The warning triangle [3] is displayed as a failure
message together with the audible signal.
3. To accept the failure message:

> Press any position on the display.
> The ERROR dialog box with the description of
the error is displayed.
4. To end the auto-start routine:

> The system returns to the main menu.
7.10 Running the contra-con routine
contra-con is an automatic decontamination routine

for disinfecting the device work space. For a detailed
description of this device function, (see chap. 9.4‚
pg. 104).
7.11 User configuration
The user configuration settings allow the user

interface and the additional device functions to be
adjusted to the requirements of everyday operation.
The user configuration menu is split into six
categories:
• Settings,
• Event logging,
Fig. 40: Interrupting the auto-start routine
• Options,
• Icon description,
50115191 B / 11.2010 76
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• Keypad lock,
• Software versions.
To make a user-specific setting in a dialog box,
navigate through the submenus listed in the
illustrations and open the dialog box.
7.11.1 Settings
The input dialogs of the SETTINGS category comprise
all settings for the individual configuration of the user
display screen and the user interface:
• Keypad lock coding,
• Date/time setting,
• Contrast setting,
• Key tone setting,
• Interface configuration,
• User display screen language setting,
• Reminder interval setting.
Changing the keypad lock code:

The keypad lock prevents the unauthorized changing
of the operational settings. Only those keys are locked
where values can be entered.
The keypad is locked by entering four numbers.
• Upon delivery, the default code is: 0000.
This default can be changed into a user-defined code
that is then enabled using the KEYPAD LOCK dialog box
(see chap. 7.11.5‚ pg. 92).
1. To enter the default 0000:

> Press the corresponding numeric keys.
> The number combination is displayed hidden in
the input box [1].
2. To delete the input numbers:

> Press the DELETE key [2] .
3. To exit the menu:

> Press the BACK key [4] .
4. To confirm the input:

> The system changes to the CODE NEW menu. Fig. 41: Changing the code
Entering the new 4-digit code:

> Press the corresponding numeric keys.
> The number combination is displayed in the
input box [1].
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Fig. 41: To set the cursor to the left to overwrite a

value:
5. Press the BACKSPACE key (<<) [6].
6. To accept and save the input value:

> The system returns to the Settings menu.
Changing the user-defined code:

The user-defined code can be changed as
often as required using the same
procedure:
• Activate the recoding function by
entering the valid code,
• enter the new code and confirm it.
Setting date / time:

The input dialog allows date and time to be set to the
required time zone.
1. Fig. 42: To enter the date:

> Press the numeric key [2].
> The input numbers are displayed in the input
box [1].
2. To set the cursor to the left to overwrite a value:

> Press the BACKSPACE key (<<) [4].

4. The system returns to the Date/Time menu.
5. Enter the time accordingly.

> The system returns to the Date/Time menu. Fig. 42: Date / time setting
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Setting the contrast:

The input dialog allows the color contrast of the
operating panel to be set within the value range of
48% to 80%.
1. To increase the value:

2. To reduce the value:

3. The value change appears in the display [1].
4. To accept and save the change:

> The system returns to the Settings/Setup
menu.
Setting the key tone:

The input dialog allows the loudness of the key tone
that sounds whenever a key is depressed to be set.
The value range is 0 to 100. The change occurs in
increments of 5%. Fig. 43: Display contrast setting



> The system returns to the Settings/Setup
menu.
Fig. 44: Key tone setting
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Setting the interface baud rate:

The input dialog allows the setting of the stepping rate
for data communication of the:
• RS232 interface,
• USB interface (optional).
The stepping rate of both interfaces can be changed
within the defined baud rates (9.600, 19.200, 38.400,
57.600 baud).
The standard stepping rate of both interfaces is 57.600
baud.
Use of HERAline:
If the devices are combined with the
HERAline accessory, the baud rate must be
set to 9,600 baud.

2. To reduce the value: Fig. 45: RS 232 interface baud rate setting

> The system returns to the Interface menu.
5. To activate the new settings:

> Browse back to the main menu.
> Wait for approx. 10 seconds and perform a
reset by switching the device off and on using
the power switch.
Fig. 46: USB interface baud rate setting
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Setting the user display screen language:

The input dialog allows the language of the display
screen to be set. Five languages are available:
• German,
• English,
• Spanish,
• French,
• Italian.
1. To browse upward in the selection:

2. To browse downward in the selection:

3. The new language appears in the display [1].
4. To accept and save the selection:

> The system returns to the Settings menu.
Fig. 47: Language setting
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Setting the reminder intervals:

The reminder intervals are integral components of the
alarm and monitoring system of the device control.
For the two essential functions contra-con and auto-
start as well as for routine service work, the user can
set dates that trigger an alarm whenever they occur.
The counting begins at 00:00 hrs of the day on which
the previously set reminder interval has elapsed.
1. To increase the number of days:

2. To reduce the number of days:

4. To deactivate the reminder interval:

> Set the value to OFF.

> The system returns to the Reminder Interval
menu.
On the due date, the display shows a reminder

message [5] for the activated reminder interval.
• contra-con: Please run contra-con.
• auto-start: Please run auto-start. Is displayed after
the contra-con decontamination routine has been
run successfully.
• Service interval: Request service. The service
message can be confirmed. In this case, the
Request Service icon is displayed.
After the routines have been run successfully, the
reminder messages are hidden.
Factory settings:
contra-con decontamination routine 90 days
auto-start routine Off
Service interval 365 days
Fig. 48: Reminder interval setting
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7.11.2 Event logging

The input dialogs of the category EVENT LOGGING
comprise all settings for logging and outputting events
during the operation of the device:
• Event display,
• time interval (of the logging cycle),
• error table.
Event display:
The event display [1] uses short, single-line entries
specifying date and time to report the events that were
logged during the operation of the device. The entries
are listed in chronological order with the most recent
event at the top position. The list can be displayed but
not edited. If the event display consists of several
pages, the user can browse through the list. The status
indicator [2] indicates which page of the total number
of pages is currently being displayed.
1. To browse forward in the list:

> Press the CONTINUE key [5].
2. To browse backward in the list: Fig. 49: Event display

> Press the PREVIOUS key [3].
3. To exit the display:

> The system returns to the Event Logger menu.
Changing the logging cycle time:

Due to limited memory resources, the oldest entries
are deleted as new entries are logged. The period from
which the displayed entries originate depends largely
on the selected logging cycle time.
Logging cycle time Minimal displayed period
10 s 22.5 hours
30 s 2.8 days
60 s 5.6 days
120 s 11.2 days
180 s 16.8 days
The setting controls the logging cycle in sections of

seconds during which the control loop measured
values are logged during the operation of the device
and displayed by the trend display (see chap. 7.12‚ Fig. 50: Logging cycle setting
pg. 94).
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Fig. 50: The settings can be made within the value

range of 10 seconds to 3600 seconds.



Event logger interval time:

The logging cycle time does not affect the
entries of the error table.
Displaying the error table: Fig. 51: Error table display

The error table lists the errors detected by the device-
integral monitoring system in descending
chronological order. The most recently detected error
is listed at the top position of 22 possible entries. An
entry consists of the control loop in which the error
occurred, the date, the time, and an error description.
The error table can be displayed but not edited.
If the event display consists of two pages, the user can
browse through the list. The status indicator [2]
indicates which page of the two is currently being
displayed.
1. To browse forward in the error table:

> Press the CONTINUE key [4].
2. To browse backward in the list:

> Press the PREVIOUS key.
3. To exit the display:

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Troubleshooting:
For a detailed overview of causes for errors
and their correction, please refer to the end
of this chapter!
7.11.3 Options
The input dialogs of the OPTIONS category comprise all
settings of the functional device options:
• Alarm relay,
• Low humidity,
• Gas-tight screen,
• Water level sensor,
• Audible alarm,
• Bottle turning device (optional),
• O2 (optional).
Setting the alarm relay:

The alarm relay is the interface for the connection of
the device-integral monitoring system to an external
monitoring system. Depending on the required input Fig. 52: Alarm relay setting
signal of the external monitoring system, network
monitoring can be enabled or disabled. If network
monitoring is enabled, a power failure is detected as
an error. The relay cannot be switched off (see chap.
5.11‚ pg. 61).
1. To toggle between two states [1]:

or

> The system returns to the Options menu.
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Setting low humidity:

If condensation occurs on the culture containers due
to high relative humidity, the humidity in the work
space can be set to a lower level. At the factory, the
device control is preset to high humidity (approx. 93%
relative humidity).
By enabling the Low Humidity feature, the relative
humidity in the work space is lowered from approx.
93% to approx. 90%. The modification requires an
extended adaption phase. To effectively prevent
condensation on culture containers, it must be used as
a permanent setting.

or
Low humidity:
The enabling/disabling of the Low
humidity function is entered into the event
list.
If the Low humidity function is enabled, the
corresponding icon [5] is displayed in the
main menu.
Fig. 53: Low humidity setting
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Setting the gas-tight screen:

As the aperture cross-sections are smaller when
accessing the samples, devices equipped with the
optional gas-tight screen achieve shorter recovery
times for the incubation parameters:
• Work space temperature,
• CO2 concentration,
• O2 concentration,
• relative humidity.
After conversion of the device, the device control

system must be adjusted to the gas-tight screen
option.
Malfunction:
The conversion to gas-tight screen causes a
change of the control parameters.
Failure to set the gas-tight screen function
in accordance with the actually installed
door version may impair the incubation Fig. 54: Gas tight screen setting
performance.
1. To toggle between two options [1]:

or

Switching the water level sensor on/off:

For incubation operation with ambient humidity or if
the auto-start routine is to be run dry (without water),
the water level sensor can be switched off.
This prevents alarm messages for the water level
sensor by the device-integral monitoring system from
being issued.

or
Fig. 55: Water level setting

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Switching the audible alarm on/off:

If the device-integral monitoring system detects an
error:
• an audible alarm sounds
• in addition to the visual error message and the
switching of the alarm relay.
The audible alarm can be permanently disabled:

or

Setting the bottle turning device speed (option

available only on HERAcell® 240 i):
If the device has been configured at the factory for the Fig. 56: Alarm relay setting
bottle turning device function, the main menu displays
the bottle turning device icon.
The speeds of the drive rollers can be set separately
for each level within a value range of 0% to 100% (see
chap. 4.10.8‚ pg. 47).
The drive roller of a bottle turning insert is activated as
soon as a value> 0 for the speed has been entered and
saved.


3. To switch the drive roller of one level off:

> Set the value to 0%.

> The system returns to the Bottle Turning Device
menu.
Fig. 57: Bottle turning device speed setting
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Quick access to bottle turning device

levels:
To directly access the Bottle turning device
submenu, touch the Bottle turning device
icon in the main menu.
Switching the O2 control on and off:

Depending on the requirements to the work process,
the O2 control can be switched on and off. This setting
is possible only on versions with the optional O2/N2
control.
1. To toggle between two states of the O2 control:

or
3. To accept and save the setting: Fig. 58: Switching the O2 control on and off

O2 value display:
If the O2 control has been switched off, the
O2 display does not show an actual value (-
- -).
This procedure provides protection for the
O2 sensor.
If the set value is set to 21%, the O2 control
loop is not monitored. This applies to both
O2 control ranges:
• Control range I: 1% to 21%
• Control range II: 5% to 90%
In this case, the O2 display shows the
actual value.
Airing the work space:

If the device was operated with O2 or N2 ,
the work space must be aired after the O2
control has been switched off.
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Gas monitoring:
The switching state of the O2 control loop
does not affect the function of the optional
gas monitoring system. The gas monitoring
system remains active even when the O2
control is switched off or the O2 sensor is
disabled.
7.11.4 Icon description

Essential operating states or error messages, e.g.
keypad lock [3], low humidity [4] or bottle turning
device [5] are displayed as icons in the touchscreen
main menu in addition to the entries in the event or in
the error table.
The Icon Description dialog box [1] explains the
meaning of the individual icons.
• To exit the display:

> The system returns to the Configuration menu.
Function of the individual icons:

Bottle turning device:
This function indicator shows that the device has been
configured for operation with the optional bottle
turning device.
The Bottle Turning Device dialog box can also be
accessed directly by touching the Bottle Turning
Device icon in the main menu.
Low water level:

This error indicator shows that the water level sensor
has detected a low water level.
Low humidity:
This function indicator shows that the relative
humidity in the work space has been lowered from
approx. 93% to approx. 90%. Fig. 59: Icon description overview
Keypad lock:
This function indicator shows that the keypad lock has
been activated so that currently the settings cannot be
changed.
Overtemperature:
This error indicator shows that the device control has
activated the overtemperature protection and has
switched to backup control.
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Gas cylinder empty:

Error message, indicating, that the filling level of one
or of serveral gas cylinders is too low to ensure regular
gas supply.
The monitoring function is available only for device
versions with the optional gas monitoring system.
Request service:
This indicator shows that the routine service is due.
The display of the icon is controlled by the time entry
in the REMINDER INTERVAL dialog box and it appears
after the reminder message has been confirmed.
Gas cylinder filling level display (optional):

If the device is equipped with the optional gas
monitoring system the icons [1] for gas cylinder A and
gas cylinder B appear in the corresponding CO2 Fig. 60: Gas monitoring icons
display / O2 display. The icons indicate the gas
cylinder filling level (full / empty).
The icon with the blue frame indicates the bottle not in
use that will be switched over to continue the supply.
• Manual changeover to a full gas cylinder is
possible.
> Press the cylinder icon with the blue frame.
or
• changeover can occur automatically, if:
> the cylinder pressure drops below 0.6 bar.
After a manual or automatic changeover of the gas

supply, another changeover is inhibited for 30
seconds. It takes approximately 2 minutes before the
filling level of the new cylinder has been determined
and can be displayed.
The gas monitoring system monitors the filling state of
the two connected gas cylinders.
If one cylinder is empty:
• no audible alarm sounds but an error message is
displayed,
• an entry is written into the event list.
If both bottles are empty:
• an audible alarm sounds and the alarm relay swit-
ches,
• an error message is displayed,
• an entry is written into the error table,
• an entry is written into the event list.
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Cylinder changeover:
A manual or automatic changeover of a gas
cylinder is entered into the event display.
7.11.5 Enabling/disabling the keypad lock

This input dialog box allows the keypad lock to be
enabled or disabled. At the factory, the keypad lock is
preset to the standard code 0000.
1. Enter the 4-digit code using the keypad. The input

appears encrypted in the display [1].
2. To delete complete incorrect input:

> Press the DELETE key [2].
3. To break off the input:

> Press the BACK key[4].
The system returns to the Configuration menu.
Fig. 61: Enabling/disabling the keypad lock
4. To confirm the input:
Changing an existing code:

The currently valid code can be redifined in
the KEYPAD LOCK CODE dialog box of the
SETTINGS/SETUP menu. (see chap. 7.11.3‚
pg. 85).
Resetting the code:

If the keypad lock code is no longer
available, the code must be reset to the
standard code by the Technical Support of
Thermo Fisher Scientific.
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7.11.6 Software versions
This menu shows the unit software versions in the

display [1].
• To exit the display:

Fig. 62: Software versions
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7.12 Scaling the trend display
The trend display of the three control loops:

• Temperature,
• CO2,
• O2,
can be scaled to two different versions.
1. Full screen display
> Press the display, for the value required.
> press the SHOW TREND ICON [1].
2. To display an enlarged section:

> Open a rectangular section [2] in the desired
area of the diagram using a finger or a pen. The
size of the rectangle is determined by dragging
a diagonal from the start (press onto screen at
left of upper diagram frame) to the end (release
at right of lower diagram frame).
> Press any position within the marked rectangle
area [2]. The section is now enlarged.
> This process can be repeated any number of
times until the section is displayed in the desi-
red enlargement or until the maximal enlar-
gement level (max. 30 event logger elements,
i.e. 30 min. process at a logging cycle of 60
seconds).
> During zoom mode the trend display can be
scrolled forward and back.
3. To return to the total trend:
> Open rectangle over a small diagram section
and press any position outside the marked
area.
4. To exit the trend display:

Fig. 63: Trend display scaling
Logging cycle:
The time interval of the data logging cycle
can be redefined in the INTERVAL TIME EVENT
LOGGER dialog box (see chap. 7.11.2‚
pg. 83).
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7.13 Error messages
The error detection system is an integral element of

the device-internal control system. It monitors the
control loops and their sensors. If an error is detected
in the system, the alarm relay switches and issues the
following signals and messages:
• An audible alarm sounds,
• a blinking warning triangle [2] and the
corresponding icon [1] are shown in the main
menu; the value displays are no longer updated,
• the detected error is listed in the error table,
• the event is entered into the event display.
7.13.1 Response to an error message event

If the alarm relay was switched due to a user action,
the switching state can be reset by accepting the error
message (e.g. for the manual cancellation of the
contra-con decontamination routine).
If the alarm relay was switched due to a techical
defect, the switching state remains active until the
defect has been corrected (e.g. low water level in work
space).
1. To accept the failure message:

> When the warning triangle [2] is displayed,
touch any position on the touch screen.
> The Error dialog box [3] apppears and the
detected error is displayed,
> the audible alarm is switched off.
2. To exit the error display: Fig. 64: Event error messages

> The error message disappears.
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7.13.2 Resetting the overtemperature protection

mechanism
If the device control has activated the over-
temperature protection mechanism and switched over
to emergency control mode, a blinking warning
triangle [2] and the icon [1] are displayed in the main
menu.
1. To display the fault cause:
> Touch any position on the touch screen.
> The Error dialog window [3] opens and the
detected fault cause is shown;
> the acoustic signal is switched off.
2. To close the error display:
> Press the END [5] key.
> The error message disappears.
> The temperature display field [4] is surrounded
by a red frame.
3. To reset the error message:
> Switch off the device.
4. Open the doors and let the work space cool
down.
5. Switch on the device.
If the overtemperature protection mechanism is
re-activated although the potential fault causes (see
error table) have been eliminated, take the device out
of service and contact the technical service center.
7.13.3 Troubleshooting
The error tables indicate source of error, cause of
error, and possible corrections.
For any communication with the Technical Support of
Thermo Fisher Scientific, please have the device data Fig. 65: Event error overtemperature
ready.
50115191 B / 11.2010 96
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Error message overview:

Control
Error message Cause Repair Alarm relay Buzzer
loop
Device door open Doors open for more than 10
System Close device doors x x
too long minutes.
Communication between Contact Service when failure
Failure display x x
display and main board faulty is recuring
Failure EEPROM EEPROM on main board is
Contact Service x x
main board faulty.
Failure Communication between
Contact Service when failure
communication display and main board faulty
is recuring
mainboard *1)
Failure during recording data Reset device to cancel failure.
System - Failure
logger. Device is still When failure is recuring x
data logger
working. contact Service.
Reset device to cancel failure.
Failure contra-con Failure in contra-con routine When failure is recuring x x
contact Service.
System - Power
Power failure during contra- Reset device and start contra-
down during contra- x x
con routine con again.
con
Failure upon auto- Failure during auto-start Reset auto-start. When failure
x x
start routine is recuring contact Service.
Reset device. When failure is
System - Safety Temperature signal
listed several times, contact
circuit active plausibility doubtful *2)
Service.
Failure Communication between
bottle turning bottle turning device and Contact Service x x
device main board faulty
Tempe- Detected value is out of

Sensor breakage Contact Service x x
rature limit.
Act. val. > nom. val. + 1°C Do not exceed ambient
Actual value above x x
*3) temperature limit
Act. val. < nom. val. - 1°C
Actual value below Contact Service x x
*4)
Calibration values Temperature max.
Contact Service x x
too high / too low adjustment value exceeded
Actual value
Temperature signal
plausibility Contact Service x x
plausibility doubtful
doubtful
Failure Measuring cell cannot
Contact Service x x
communication\ commuicate with mainboard
*1) Error message will be displayed, but not entered in error table.
*2) Error message will be displayed, but not entered in error table.
*3) If failure is detected, a special sample protection controlling will be enabled. To indicate the start of this routine,
the icon overtemperature is displayed. The routine will will be disabled after a power reset.
*4) After changing the set value, error time will be set on 159 min., after door opening on 45 min. ( O2 on 159 min.).
50115191 B / 11.2010 97
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Control
loop
LM75 sensor cannot not
Tempe-
Failure LM75 communicate with Contact Service x x
ratur
mainboard
Failure EEPROM
NV-RAM sensor faulty Contact Service x x
sensor
Detected value is out of Reset auto-start. When failure

CO2 Sensor breakage x x
limit. is recuring contact Service.
Act. val. > nom. val. + 1% Check gas supply. Reduce
*4) prepressure to 1 bar max.
Check gas supply. Change
Act. val. < nom. val. - 1% gas cylinder. Raise
Actual value below x x
*4) prepressure to 1 bar max..
Check supply line to device.
Calibration values Max. adjustment value
Contact Service x x
too high / too low exceeded for CO2
Failure Measuring cell cannot
Contact Service x x
communication commuicate with mainboard
Failure gas Gas cylinder changeover
cylinder switch cannot commuicate Contact Service x x
changeover switch with mainboard
Replace one or both O2
No gas Both CO2 cylinders empty x x
cylinders.
Gas cylinder A
Gas cylinder A is empty. Replace gas cylinder A.
empty
Gas cylinder B
Gas cylinder B is empty. Replace gas cylinder B.
empty
Failure EEPROM
NV-RAM sensor error. Contact Service x x
sensor
Detected value is out of

O2 Sensor breakage Contact Service x x
limit.
Act. val. > nom. val. + 1% Check gas supply. Reduce
*4) prepressure to 1 bar max.
Check gas supply. Change
Act. val. < nom. val. - 1% gas cylinder. Raise
Actual value below x x
*4) prepressure to 1 bar max..
Check supply line to device.
Failure O2 sensor cannot
Contact Service x x
communication commuicate with mainboard
*4) After changing the set value, error time will be set on 159 min., after door opening on 45 min. ( O2 on 159 min.).
50115191 B / 11.2010 98
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Control
loop
Gas cylinder changeover
Failure gas cylinder
O2 switch cannot commuicate Contact Service x x
changeover switch
with mainboard
Replace one or both O2
No gas Both CO2 cylinders empty x x
cylinders.
Gas cylinder A
Gas cylinder A is empty. Replace gas cylinder A.
empty
Gas cylinder B
Gas cylinder B is empty. Replace gas cylinder B.
empty
Fill in water or if the auto-start

routine is to be run dry
Water level in the incubator
rH No water (without water), the water x x
under
level sensor can be switched
off.
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8 Shut-down
8.1 Shutting the device down
Contamination hazard!
If the work space surfaces are contaminated, germs can be
transferred to the environment of the device.
In case of a shut-down, the device must be decontaminated!
1. Remove the culture containers and all accessories from the work
space.
2. Pump the water out off the water reservoir (see chap. 9).
3. Fill in 350 ml water and start the contra- con decontamination routine.
4. Turn the device off using the power switch when the contra-con
decontamination routine has been completed.
5. Unplug the power connector and protect it against accidental
reconnection.
6. Close the CO2/O2/N2 supply system shut-off valves.
7. Disconnect the gas pressure hoses from the sleeve at the rear of the
device.
8. Clean the work space and wipe the device dry.
9. Until the device is shut down, the work space must be continuously
ventilated. Leave the glass door and the outer door open and secure
them in this state.
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9 Cleaning and disinfection
9.1 Cleaning
Incompatible cleaners!
Some device components are made of plastic. Solvents can
dissolve plastics. Strong acids or caustic solutions can cause to
become brittle of the plastic.
For cleaning plastic components and surfaces, do not use
solvents that contain hydrocarbons, solvents with an alcohol
content of more than 10% or strong acids or caustic solutions.
Moisture-sensitive components!
Do not spray cleaning agent onto the touchscreen and the
control box at the rear of the device. When wiping the device
clean, always make sure that moisture does not enter into
these components.
Exterior surfaces Cleaning exterior surfaces:

1. Remove dirt residues and depositions thoroughly using a solution of
water and commercial detergent.
2. Wipe the surfaces clean using a clean cloth and clear water.
3. Then, wipe the surfaces dry using a clean cloth.
Cleaning display:
Moisture-sensitive display!
Do not spray or wipe the display with cleaner.
• Clean display using a dry cloth of 100% micro fiber!
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9.2 Decontamimation procedures
The operator must prepare hygiene regulations for the decontamination of

the device in accordance with the application of the device.
Disinfection procedure The following disinfection procedures are compatible with the device:
Wipe/spray disinfection:
The wipe/spray disinfection is used as the standardized manual disinfection
procedure for the device and for all accessories.
contra-con decontamination routine:

The contra-con decontamination routine uses an automated program cycle
to decontaminate the complete work space including the shelf system and
the sensors.
9.3 Wipe/spray disinfection
The manual wipe/spray disinfection is carried out in three stages:

Disinfection stages • Predisinfection,
• cleaning,
• final disinfection.
Alcoholic disinfectants!
Disinfectants with an alcohol content of more than 10% may
form, in combination with air, easily combustible and explosive
gas mixtures.
When using such disinfectants, avoid open flames or exposure
to excessive heat during the entire disinfection process!
• Use such disinfectants only in adequately ventilated rooms.
• After the disinfectant has been allowed to react, wipe the
cleaned device components thoroughly dry.
Observe safety regulations to avoid fire and/or explosion
hazard caused by alcohol-containing disinfectants (ZH 1/598).
Chloride-containing disinfectants!
Chloride-containing disinfectants can corrode stainless steel.
• Use only disinfectants that do not affect stainless steel!
50115191 B / 11.2010 102

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Preparing the manual wipe/spray disinfection:
Electric shock!
Contact with current-carrying components may cause a lethal
electric shock.
Prior to cleaning and disinfection work, disconnect the device
from the power supply!
• Turn the device off using the power switch.
• Unplug the power connector and protect it against
accidental reconnection.
• Check to see if the device is deenergized.
Health hazard!
The surfaces of the work space may be contaminated. Contact
with contaminated cleaning liquids may cause infections.
Disinfectants may contain harmful substances.
When cleaning and disinfecting, always observe the safety
instructions and hygiene regulations!
• Wear safety gloves.
• Wear safety goggles.
• Wear mouth and respiratory system protection gear to
protect the mucous membranes.
• Observe the safety instructions of the manufacturer of the
disinfectant and of the hygiene experts.
Predisinfection:
Predisinfection 1. Remove all samples from the work space and store them in a safe
place.
2. Pump the water off and remove water residues using a cloth.
3. Spray disinfectant onto the surfaces of the work space and of the
accessories or wipe the surfaces clean using disinfectant.
Moisture-sensitive components!
Do not spray disinfectant onto the CO2 sensor in the measuring
cell baseplate or onto the O2/N2 sensor.
4. Allow the disinfectant to react as specified by the manufacturer.
Removing accessories and shelf system:

1. Remove gas humidification, bottle turning device and/or shelves, then
remove the entire shelf system from the work space.
For removal and installation of the shelf system, please refer to
Section 5.2.
2. If required, remove the blower wheel and its cover from the baseplate
50115191 B / 11.2010 103

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of the measuring cell. The wheel and the cover can be autoclaved.
Removing blower wheel and cover:
Electric shock!
Contact with current-carrying components may cause a lethal
electric shock.
Prior to removing the blower wheel, switch the device off and
disconnect it from the power supply system.
1. Remove the two retaining screws of the cover using the supplied Allen
wrench (3 mm) and remove the cover.
2. The blower wheel is secured to the axle by a set screw. Remove the
set screw using the Allen wrench (2 mm) and pull the blower wheel
off.
Cleaning the work space and accessories:

1. Thoroughly remove dirt residues and deposits using a solution of
tepid water and dishwashing agent.
2. Wipe the surfaces clean using a clean cloth and plenty of clear water.
3. Remove the cleaning liquid from the water tray and wipe all surfaces
of the work space thoroughly dry.
4. Wipe accessories thoroughly dry.
Final disinfection:
Final disinfection 1. Spray disinfectant again onto the work space, the shelf system, and
the removed components and wipe them clean.
2. Allow the disinfectant to react as specified by the manufacturer.
3. Reinstall the shelf system and the removed components.
Functional check:
After the installation, check to see if the blower wheel is
securely attached to the axle and if it can rotate freely, then
secure cover using the screws.
50115191 B / 11.2010 104

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9.4 contra-con decontamination routine
The entire program run of the decontamination routine takes

approximately 25 hours.
contra-con process During this routine, a hot and humid atmosphere at 90 °C with highly
decontaminating effect is created in the work space. The effectiveness of
the contra-con decontamination routine has been certified by independent
institutes. Information on these tests is available from Thermo Scientific
upon request.
After the run has been completed, the device must be started up again
using the auto-start routine.
Conditions that prevent the start of the contra-con

decontamination routine:
The contra-con routine cannot be started if one of the following
failure conditions exists.
Temperature control loop:
• Sensor breakage,
• actual value above set value (excessive deviation),
• actual value below set value (excessive deviation),
• set value not plausible,
• calibration values too high or too low,
• sensor communication failure,
• sensor parameter not plausible,
• no communication with LM 75.
Control loop for CO2 gas supply:

• No communication with sensor.
In this case, the conta-con key is dimmed and its function is not
available.
Overtemperature protection:
If the overtemperature protection was enabled on the device,
the contra-con decontamination routine cannot be started
before the fault hes been corrected or reset.
No gas is supplied during the contra-con decontamination

routine on versions with the optional gas monitoring system:
If the error „no gas“ occurs during the contra-con
decontamination routine, the audible alarm is triggered. The
alarm can be confirmed by pressing on any position on the
display. In this case, the contra-con decontamination routine is
not cancelled. The alarm relay remains switched until the gas
monitoring system detects a full gas cylinder again.
50115191 B / 11.2010 105

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Optional bottle turning device:

Before starting the contra-con decontamination routine, all
rollers must be removed from the incubator. The electrical
connection sockets must then be sealed with the protecting
caps.
The rack that supports the rollers may be left in place for the
duration of the cycle.
Procedure of a contra-con decontamination routine:

contra-con procedure 1. After cleaning, reinstall the shelf system components into the work
space.
2. Fill the floorpan with 350 ml processed water.
4. Activate and start the decontamination routine.
5. After the decontamination routine has been completed, remove the
remaining water using a sterile cloth.
6. Turn the device off or resume operation.
Hot surfaces!
The handle and the screen of the glass door, the interior panel
of the outer door as well as the surfaces of the shelf system and
of the work space become extremely hot during the contra-con
decontamination routine.
During the routine run or immediately after completion of the
run, always wear safety gloves when touching these surfaces!
Damage to the samples!

During the contra-con decontamination routine, the work
space is heated up to 90 °C.
Make sure that:
• all samples are removed from the work space,
• all accessories are removed from the work space.
50115191 B / 11.2010 106

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Operating phases of contra-con decontamination:

The remaining run time of the contra-con
decontamination routine describes the time between
the start or the current time status to the end of the
drying phase. The indicated remaining run times are
not measured values but merely used for orientation.
The routine is divided into five phases:
1. Heating phase,
2. decontamination phase,
3. condensation, Fig. 66: Decontamination routine phases
4. cool-down,
5. drying.
Heating phase: Remaining run time approx. 25 hours.

The work space is heated to a temperature of 90° C
while an elevated relative humidity is created.
Decontamination phase: Remaining run time approx

23 hours.
After the decontamination atmosphere has been
created, the decontamination phase of approx. 9
hours is started. The temperature is maintained at
90°C.
Condensation: Remaining run time approx. 14 hours.

The floor heating is switched off, the temperature is
maintained only by the sidewall heating. This function
is activated to reduce the created moisture before the
cool-down and to collect it at the work space floor.
Cool-down phase: Remaining run time approx. 8

hours.
The device cools down until the originally set
temperature set value is reached.
Postheating phase: Remaining run time approx. 1

hour
During the postheating phase, condensate within the
device is eliminated as far as possible; remaining
condensate accumulates at the bottom of the work
space.
.
End of the decontamination routine: Remaining run
time 0 hours
When the remaining run time has elapsed to 0 hours,
the device has reached the originally set working
temperature again (e.g. 37 °C). The contra-con
decontamination routine must then be stopped by
pressing the appropriate key.
50115191 B / 11.2010 107

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9.4.1 Activating the contra-con routine

Fig. 67: The contra-con decontamination routine is
used to decontaminate the complete work space .
1. Press the CONTRA-CON key [1].

> The contra-con-instruction menu [2] is
displayed.
2. To exit the contra-con-instruction menu and

contra-con:
3. To activate contra-con:
> Press the START key [3].
> The contra-con-instruction menu [2] is
displayed.
4. To air the work space, open both device doors

when the audible alarm sounds after 30 seconds.
5. Remove all samples from the work space.
6. Pump the water out of the floorpan and wipe off

water residues.
7. Fill the floorpan of the work space with 350 ml

processed water.
8. Close both device doors.

> The run of the contra-con decontamination
routine starts.
> Fig. 68: While the contra-con decontamination
routine is running, the display shows the
current status [5] and outputs the following
information:
– Temperature,
– start time,
– phase,
– remaining run time.
9.4.2 Interrupting contra-con

Fig. 67: The contra-con decontamination routine can
be interrupted at any time.
1. To interrupt contra-con:
> Press the STOP key [6].
Fig. 67: Activating contra-con
50115191 B / 11.2010 108

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If the STOP key is pressed, the routine is interrupted

and the contra-con Stop dialog box is opened as a
security request. The routine can now be ultimately
cancelled or resumed.
2. To cancel contra-con:
> The error message is displayed.
> If the error message is confirmed, the system
returns to the main menu.
3. To resume contra-con:
> Press the BACK key [1].
> The system returns to the status display, and
the decontamination routine is resumed.
4. To interrupt contra-con from the status display:

> Press the STOP key [4].
> The contra-con Stop dialog box is displayed as
a security request (see above). Proceed with
work step 2. (see section above).
9.4.3 contra-con interruption due to error

If an error occurs while the decontamination routine is
run, an error message [3] is displayed and the
following actions are initiated:
– The decontamination routine automatically
changes to the cool-down phase,
– the audible alarm sounds.
1. To confirm the audible alarm:
> Press any position on the display.
> The audible alarm is switched off. The END key
is displayed.
If the decontamination routine is not cancelled
after this, cool-down to the set temperature
occurs.
2. To cancel contra-con:
> The error message is displayed.
> If the error message is confirmed, the system
returns to the main menu.
Fig. 68: Interrupting / cancelling contra-con
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9.4.4 Completing contra-con

After the five phases have been completed, the Stop
CONTRA-CON DECONTAMINATION ROUTINE dialog box [1]
is displayed.
The decontamination routine must be stopped
manually.
• To stop contra-con:
Opening door during contra-con

decontamination:
If the door will be opened and closed again
whilst contra-con decontamination is
running, the routine returns to a phase
Fig. 69: contra-con completion
which will ensure a continuation without
faults.
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10 Maintenance
10.1 Inspections and checks
To ensure the operativeness and the operational safety of the device, the
functions and device components listed below must be checked at
different intervals.
Daily check:
• Gas supply of the CO2 supply system.
• Gas supply of the O2/N2 supply system.
Annual inspection:
• Tightness of the glass door seal.
• Permeability of the pressure compensation opening with insert.
• Functional check of the operating panel and of the device control.
• Electrical safety check in accordance with the relevant national
regulations (e.g. VBG 4).
Functional check:
If safety devices were removed or disabled for inspections, the
device must not be operated before the safety devices have
been reinstalled and checked for their correct function.
10.2 Service intervals
During running operation, the following service works must be performed:
3-month service:
• Run auto-start routine and contra-con decontamination routine,
• perform temperature and CO2/O2 comparison measurement.
Annual service:
• Replace gas inlet filter.
• Perform Technical Service service inspection.
Service contract:
Thermo Scientifc offer a device-specific service contact that
comprises all test and service works required.
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10 Maintenance
10.3 Preparing the temperature calibration
To determine the exact measured value of the device-

integral temperature sensor, a temperature
comparison measurement has to be performed every
three months.
If a major temperature deviation is found during this
check, a temperature calibration is required.
During this process, the temperature control of the
device is set to the value measured during the
temperature comparison measurement.
Use a calibrated measuring instrument with an
accuracy of < ± 0,1 °C for this test. To minimize
temporary temperature fluctuations during the
measurement, the measuring instrument is placed
into the work space in an isothermal container (e.g. a
bowl filled with glycerol). The center of the work space
is the reference location for the comparison
measurement.
Isothermal container:
Do not use a container filled with water as
isothermal container as the evaporation of
water will result in a lower temperature Fig. 70: Preparing the temperature calibration
reading.
Excessive work space temperature:

Excessive work space temperature after
the calibration can be reduced by leaving
the doors open for approx. 30 seconds.
Comparison measurement procedure:

2. Set the temperature set value and allow the
device to be heated. This may take up to several
hours.
3. Place the measuring instrument [3] onto the
center area of the work space.
Alternatively, a temperature sensor may be
positioned in this location. Route the connecting
cable either through the measurement opening
[2] in the glass door or through the access port [1]
in the rear panel of the device.
50115191 B / 11.2010 112

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10 Maintenance
4. Close the doors.

5. Wait until the temperature value displayed on the
measuring instrument has stabilized.
6. Calibrate the temperature control as described in
(see chap. 10.4‚ pg. 113)
10.4 Temperature calibration procedure
Measurement example:
• Temperature set value: 37 °C
Reference temperature: 36.4 °C
1. Press TEMPERATURE DISPLAY [1].

> The temperature menu is displayed.
2. To exit the temperature menu:

3. To enter the Calibration submenu:

> Press the CALIBRATION key [2].
4. Enter the measured value (targeting value):

The targeting value can be increased or reduced
in increments; if you keep the + key [4] or the –
key [6] depressed, the function switches to a rapid
increase/reduction; after approx. 3 seconds,
another increase/reduction occurs.
To increase the targeting value:


> Press the – key [4].
5. To accept and store the targeting value:

temperature display shows the current actual
value measured in the work space.
Excessive work space temperature:

Excessive work space temperature after
the calibration can be reduced by leaving
the doors open for approx. 30 seconds.
Fig. 71: Temperature calibration procedure
.
50115191 B / 11.2010 113

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10 Maintenance
Value reset:
If the value isn’t changed within the next 30
seconds, the system automatically exits
the menu, and the most recently confirmed
value is preserved.
10.5 Preparing the CO2 calibration
To determine the exact measured value of the device-

integral CO2 sensor, a CO2 comparison measurement
has to be performed every three months.
If a major deviation is found during this check, a CO2
calibration is required.
During this process, the CO2 control of the device is
set to the value measured during the comparison
measurement.
Use a calibrated measuring instrument with an
accuracy of < ± 0,3 % CO2 for this test.
Suitable instrument:
• Portable IR readout instrument.
The measuring sample is withdrawn through the

sealable measurement opening of the glass door. The
comparison measurement must be performed when Fig. 72: Preparing CO2 calibration
the device is completely heated up.
Comparison measurement procedure:

2. Set the CO2 set value and start the device with
auto-start.
3. Insert the measuring instrument probe through
the measurement opening [1] into the work
space. Wait until the CO2 value displayed by the
instrument has stabilized.
50115191 B / 11.2010 114

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10 Maintenance
4. For devices equipped with the optional gas-tight

screen, the measurement opening is located:
– on HERAcell® 150 i [1] in the center gas-tight
screen,
– on HERAcell® 240 i [2] in the left center gas-
tight screen.
5. Remove the measuring probe, plug the
measurement opening and close the doors.
6. Calibrate the CO2 control.
IR measuring cell:
For devices with infrared (IR) measuring
cells, the CO2 calibration can only be
performed when the CO2 concentration has
been set to 4.0% or more.
Calibration should be performed with the
CO2 set value designated for the work
process (prospective work process value).
Fig. 73: Gas-tight screen measurement opening
50115191 B / 11.2010 115

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10 Maintenance
10.6 CO2 calibration procedure
Measurement example:
• CO2 set value: 5 %
Reference temperature: 5,6 %
1. Press the CO2 DISPLAY key [1].

> The CO2 menu is displayed.
2. To exit the CO2 menu:

3. To enter the Calibration submenu:

> Press the CALIBRATION key [2].
4. To enter the measured value (targeting value):

The targeting value can be increased or reduced
in increments; if you keep the + key [4] or the –
key [6] depressed, the function switches to a rapid
increase/reduction; after approx. 3 seconds,
another increase/reduction occurs.
To increase the targeting value:


> Press the – key [4].
5. To accept and store the targeting value:

temperature display shows the current actual
value measured in the CO2 display.
Excessive CO2 content:

Excessive CO2 content after the calibration
can be reduced by leaving the device doors
open for approx. 30 seconds.
Value reset:
If the value isn’t changed within the next 30
seconds, the system automatically exits
the menu, and the most recently confirmed
value is preserved.
Fig. 74: CO2 calibration procedure
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10 Maintenance
10.7 Replacing the gas inlet filter
Fig. 75: The gas inlet filter (CO2-/O2-/N2 supply) has

plastic threads and is screwed by hand into the
threaded hole at the control box.
Procedure for gas supply gas inlet filter:

1. Make sure that the gas supply is shut off.
2. Loosen the hose clamp [4].
3. Remove the gas hose [5] from the sterile filter
sleeve [2].
Procedure for all gas inlet filters:

4. Remove the retainer [1].
5. Unscrew the gas inlet filter [2] from the threaded
hole [3]. Fig. 75: Gas inlet filter installation
6. When installing the new gas inlet filter, make sure
that the plastic thread is not canted. Install the
filter using caution.
7. Install the retainer [1].
Procedure for gas supply gas inlet filter:

8. Connect the gas hose to the gas inlet filter sleeve
and secure it using the hose clamp. Check to see
if the gas hose is securely seated on the sleeve.
10.8 Replacing the device fuses
Fig. 76: The two identical device fuses [4] are installed
in the fuse compartment [1] next to the power plug
receptacle of the device:
• Time delay fuses, 6.3 A (5x20 mm)
1. The fuse holder is secured to the fuse Fig. 76: Device fuse replacement
compartment [1] using two locking tabs [2].
2. To remove the fuse holder, squeeze the two
locking tabs and pull the holder [3] out of the fuse
compartment.
3. Remove the faulty fuse from the fuse holder and
install the new fuse.
4. Slide the fuse holder into the fuse compartment
and press the holder on until the locking tabs are
fully engaged.
50115191 B / 11.2010 117

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10 Maintenance
10.9 Replacing the door seal
The door seal (magnetic seal) [3] of the outer door is

located in the retaining slot. No tools are required to
replace the seal.
1. Pull the magnetic seal [3] out of the guide slot [1].
2. Position the new seal at a corner [2] and press the
seal retaining rail [4] into the slot.
3. Make sure that the retaining rail taper is
positioned correctly in the slot [1] and that the
seal is flush with the door frame.
Fig. 77: Magnetic door seal replacement
50115191 B / 11.2010 118

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11 Disposal
Contamination hazard!
The device may have been used for treating and processing
infectious substances. Therefore, the device and device
components may have been contaminated.
Prior to disposal, all device components must be
decontaminated.
Decontamination measures • Clean the device components thoroughly and either
disinfect or decontaminate them (depending on application).
• Attach a declaration of non-objection with details of
performed decontamination measures to the items that are
to be disposed of.
Controlled disposal All device components can be disposed of after they have been
decontaminated properly.
Recycling Service
Recycling Service
Thermo Fisher Scientific offer a recycling service for discarded
components at the owner’s expense.
50115191 B / 11.2010 119

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11 Disposal
Overview of the materials used:

Component Material
Thermal insulation components Polystyrene foam EPS/PPS-Compound
Enclosed electrical components coated with different
Printed circuit boards
plastics, equipped on epoxy resin-bound boards.
Plastic components, general Note material labelling
Exterior housing Galvanized steel sheet, painted
Device rear panel Galvanized steel sheet
Outer door Galvanized steel sheet, painted
Door inner panel Galvanized steel sheet, painted
Operating panel and indicator foil Polyethylene
Magnetic door seal Magnetic core sheathed with EMPP
Heating Silicone-sheathed resistance-type wires
Stainless steel 1.4301,
Interior containers, installed components and shelves Stainless steel 1.4301 with silver ion coating (iONGUARD™)
Copper
Plug for pipe channel Silicone
Pressure compensation opening insert POM with brass sinter filter
Glass screen Soda-silicate glass
Glass door seal, measurement opening Tempered silicone
Sensor block Stainless steel 1.4301
Blower wheel Stainless steel 1.4305 or copper
Measuring cell base plate seal Tempered silicone
Cables Plastic-sheathed copper flexible
Packaging Corrugated board, polyethylene film, and styrofoam
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12 Technical data
HERAcell® 150i
Description Unit Value

Mechanical
External dimensions (W x H x T) mm 637 x 867 x 782
Interior dimensions (W x H x T) mm 470 x 607 x 530
Chamber volume l approx 151
Shelves (W x T) mm 423 x 465
Standard quantity Piece 3
Maximal quantity Piece 10
Maximal surface load kg 10 / insertion shelf
Maximal device overall load kg 30
Weight, without accessories kg 70
Thermal
Class 3.1
(overtemperature controller
Thermal safety devices according to DIN 12880:2007-05
(TWW) with overtemperature
detection function)
Ambient temperature range °C +18...33
Temperature control range °C RT + 3 ... 55
Temperature deviation, time (DIN 12880, Part 2) °C ± 0.1
Temperature deviation, spatial (DIN 12880, Part 2)
°C ± 0.5
at 37 °C *1)
Duration of the auto-start routine, to 37 °C
h 5 ... 10
ambient temperature 20 °C
Heat transfer to environment:
kWh/h 0.06
at 37 °C
kWh/h 0.112
during contra-con decontamination
Humidity
resistivity 50 kΩ to 1 MΩ,
Water quality
conductivity 1 to 20 μS
Liquid quantity:
l max. 3.0 / min 1.2
Incubation operation
ml 350
contra-con disinfection operation
Constant humidity at 37 °C (high-humidity mode) % rH approx 93
Constant humidity at 37 °C (low-humidity mode) % rH approx 90
Others
Sound pressure level (DIN 45 635, Part 1) dB(A) < 50
Relative humidity of environment % rH max. 80
Location elevation m ASL max. 2000
*1) determined on the basis of DIN 12880 for devices with standard equipment. See detailed information in calbration
instructions.
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12 Technical data
HERAcell® 150i

CO2 gas supply system
Gas purity % min. 99.5 or medical quality
Prepressure bar min. 0.8 - max. 1
Measuring and control range % vol. 0... 20
Control deviation, temporal % vol. ± 0.1
CO2 measuring cell
Accuracy (absolute) % CO2 ± 0.3
O2 gas supply system
Measuring and control range % vol. 1... 21 or 5...90
O2 measuring cell
± 0.5 (Option: 1.....21 % O2)
Accuracy (absolute) % O2
± 2.0 (Option: 5.....90 % O2)
Electrical system
V 1/N/PE 230 V, AC (± 10%)
Rated voltage V 1/N/PE 120 V, AC (± 10%)
V 1/N/PE 100 V, AC (± 10%)
Rated frequency Hz 50/60
Interference suppression (DIN VDE 0875) Interference level N
Type of protection (DIN 40 050) IP 20
Protection class l
Overvoltage category (EN 61010) ll
Pollution severity (EN 61010) 2
2.5 (230 V, AC)
Rated current A 5.2 (120 V, AC)
6.2 (100 V, AC)
On-site fusing:
Fuse T 10 A
Circuit breaker EU: B 16 / USA: N NN
kW 0.58 (230 VAC)
Rated input kW 0.62 (120 VAC)
kW 0.62 (100 VAC)
EMC class B
50115191 B / 11.2010 122

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12 Technical data
HERAcell® 240i

Mechanical
External dimensions (W x H x T) mm 780 x 934 x 834
Interior dimensions (W x H x T) mm 607 x 670 x 583
Chamber volume l approx 238
Shelves (W x T) mm 560 x 500
Standard quantity Piece 3
Maximal quantity Piece 12
Maximal surface load kg 10 / insertion shelf
Maximal device overall load kg 30
Weight, without accessories kg 81
Thermal
Class 3.1
(overtemperature controller
Thermal safety devices according to DIN 12880:2007-05
(TWW) with overtemperature
detection function)
Ambient temperature range °C +18...33
Temperature control range °C RT + 3 ... 55
Temperature deviation, time (DIN 12880, Part 2) °C ± 0,1
Temperature deviation, spatial (DIN 12880, Part 2)
°C ± 0.5
at 37 °C *1)
Duration of the auto-start routine, to 37 °C
h 5 ... 10
ambient temperature 20 °C
Heat transfer to environment:
at 37 °C kWh/h 0.07
during contra-con decontamination kWh/h 0.25
Humidity
resistivity 50 kΩ to 1 MΩ,
Water quality
conductivity 1 to 20 μS
Liquid quantity:
Incubation operation l max. 4.5 / min 1.8
contra-con disinfection operation ml 350
Constant humidity at 37 °C (high-humidity mode) % rH approx 93
Constant humidity at 37 °C (low-humidity mode) % rH approx 90
EMC class B
Others
Sound pressure level (DIN 45 635, Part 1) dB(A) < 50
Relative humidity of environment % rH max. 80
Location elevation m ASL max. 2000
*1) determined on the basis of DIN 12880 for devices with standard equipment. See detailed information in calbration
instructions.
50115191 B / 11.2010 123

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12 Technical data
HERAcell® 240i

CO2 gas supply system
Measuring and control range % vol. 0... 20
Control deviation, temporal % vol. ± 0,1
CO2 measuring cell
Accuracy (absolute) % CO2 ± 0.3
O2 gas supply system
Measuring and control range % vol. 1... 21 or 5...90
O2 measuring cell
± 0.5 (Option: 1.....21 % O2)
Accuracy (absolute) % O2
± 2.0 (Option: 5.....90 % O2)
Electrical system
V 1/N/PE 230 V, AC (± 10%)
Rated voltage V 1/N/PE 120 V, AC (± 10%)
V 1/N/PE 100 V, AC (± 10%)
Rated frequency Hz 50/60
Interference suppression (DIN VDE 0875) Interference level N
Type of protection (DIN 40 050) IP 20
Protection class l
Overvoltage category (EN 61010) ll
Pollution severity (EN 61010) 2
2.8 (230 V, AC)
Rated current A 5.4 (120 V, AC)
6.5 (100 V, AC)
On-site fusing:
Fuse T 10 A
Circuit breaker G 16
kW 0.64 (230 V, AC)
Rated input kW 0.65 (120 V, AC)
kW 0.65 (100 V, AC)
50115191 B / 11.2010 124

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13 Annex: Data communication
13.1 Interfaces
13.1.1 RS 232 interface

RS 232 configuration The RS 232 data communication interface has been designed for a cable
connection with 9-pin connector and a contact assignment of 1:1.
Setting the transmission speed:
• 9,600 – 57,600 baud,
• 8 data bits,
• 1 stop bit,
• no parity.
structure (see below).
13.1.2 USB port (optional)

USB 1.1 configuration As an alternative to data communication via RS 232 interface, the devices
can be equipped with the optional USB port. The USB port complies with
Standard USB 1.1 and is compatible with Standard USB 2.0 (full speed).
The USB port is operated as a virtual COM port. Therefore, the port
transmission speed can be changed within the defined baud rates (9,600,
19,200, 38,400, 57,600 baud).
structure. The command sequences comply with the design of the RS 232
interface.
Installing the USB port with the virtual COM port:

If the USB port is to be used for data exchange between PC and
incubator, the USB port is installed as virtual COM port (USB
serial port) using the supplied driver.
The assigned COM port can be located in the Windows Device
Manager/Ports dialog box, e.g. USB Serial Port (COM5), and is
then defined as communication port in the Heracell 150 i & 240 i
program (see chap. 13.8‚ pg. 146).
The driver can be run under the following operating systems:

WIN 2000, WIN XP, WIN VISTA.
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13.1.3 Installing the USB port driver

Connect the USB cable (optional) to the control box of the HERAcell® 150 i
/ 240 i and to a PC.
As soon as the Windows Hardware Detector has identified the USB port,
the FIND NEW HARDWARE WIZARD dialog box opens.
Windows dialog box 1. Select the INSTALL FROM A LIST OR SPECIFIC LOCATION (ADVANCED) option.
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2. Select the data CD as source.
3. On the data CD, select the DRIVER sub-directory.
4. The installation routine installs the driver: EVAL22 Board USB.

After the installation has been completed successfully, the routine is
completed with FINISH.
The interface transmission speed can be selected within the defined
baud rates (9,600, 19,200, 38,400, 57,600 Baud) at the touchscreen of
the HERAcell® 150 i / 240 i (see chap. 7.11.1‚ pg. 77).
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13.2 Structure of the command sequences for data

communication
All characters sent and received in the data exchange between a PC and the
incubator HERAcell® 150 i / 240 i are ASCII characters that can be displayed
on a conventional terminal.
This ensures that communication can be set up, controlled, and
programmed easily.
13.2.1 Description of the protocol
Protocol characteristics Character coding

ASCII characters, capital letters are not allowed.
Reading parameters:
Query: ?:aaaa:bb::cc<CR>
or: ?:aaaa:bb:XXXX:cc<CR>
Response: !:aaaa:bb:XXXXX:cc<CR>
where: aaaa = parameter address
bb = amount of payload in this telegram (00 – ff)
cc = checksum: Inverted XOR of all bytes without
checksum and <CR>
XXXX = bb payload bytes
Description of the response elements:

aaaa parameter address
bb amount of payload in this telegram (00 – ff)
cc checksum: Inverted XOR of all bytes without
checksum and <CR>
Example of a software version query (50111927):

Query: ?:0001:00::cc<CR>
Response: !:0001:08:50111927:cc<CR>
Writing parameters:
Command: !:aaaa:bb:XXXXX:cc<CR>
Response: !:aaaa:bb::cc<CR>
where: aaaa = parameter address
bb = amount of payload in this telegram (00 – ff)
cc = checksum: Inverted XOR of all bytes without
checksum and <CR>
XXXX = bb payload bytes
Response with an error message:

Response: !:aaaa:bb:XX:cc<CR>
Description of the response elements:

aaaa parameter address,
bb amount of payload (always 02)
cc checksum: Inverted XOR of all bytes without
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checksum and <CR>

XX = 2 bytes error message (see table below)
Example of an unknown command:

Query: ?:0005:00::cc<CR>
Response !:0005:02:?1:cc<CR>
Significance of the two bytes in the error mesage:
Error message Description

?0 Error in telegram structure or checksum
?1 Unknown command or unknown parameter
?2 Internal memory error
?3 Data error (value not within its limits)
13.3 Overview of general parameters (addresses 0xxx)
General parameters General parameters are system values such as date, time, and
motherboard version number.
13.3.1 Reading general parameters
Address Description Note

0001 Motherboard version number 8 digits
Output date and time
17 bytes / decimal value in
0010 [hours:minutes:seconds],
format xx:xx:xx;xx:xx:xx
[day:month:year]
Date 8 bytes / decimal value in
0011
[day:month:year] format xx:xx:xx
Time 8 bytes / decimal value in
0012
[hours:minutes:seconds] format xx:xx:xx
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13.4 Overview of incubator parameters (addresses 2xxx)
Incubator parameters Incubator parameters are divided into:

• the basic parameters of the three control loops temperature, CO2, and
O2,
• the internal function parameters of operating functions and data
logging.
13.4.1 Reading basic parameters

Device status*1) (error) status 33 bytes / hexadecimal value
of control loops for in format
2000
temperature, xxxxxxxx;xxxx; ...
CO2, O2, rh, ref. temp. ;xxxx;xxxx;xxxx
Set, actual, and reference
2010 format
temperature*2)
+xxx.xx;+xxx.xx;+xxx.xx
2020 Set and actual CO2 content*2)
format +xxx.xx;+xxx.xx
2030 Set and actual O2 content*2)
format +xxx.xx;+xxx.xx
Actual water level 7 bytes / decimal value in
204a
(100% or 0%) format +xxx.xx
Low humidity indicator (1 2 bytes / hexadecimal value in
204b
active, 0 inactive) format xx
*1) Example of device status and (error) status of control loops
(details see error message table)
*2) All values have 2 decimal places
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13.4.2 Reading internal function parameters

Reading internal functions
Status of run*1) and remaining

run time [hours:minutes] for
2100 format
disinfection, also date and time
xx;+xxx:xx;xx.xx.xx;xx:xx
of last start
Status of run*1) , current CO2 25 bytes / decimal value in

offset + waiting period format
2105
[minutes:seconds] autostart, xx;xx.x;+xxx:xx;xx.xx.xx;xx:
also date and time of last start xx
Read bottle turning device 8 bytes / hexadecimal
2132
speed (all levels)*2) value in format xxxxxxxx
Read bottle turning device
8 bytes / hexadecimal
2133 status (all levels) (1 active, 0
value in format xxxxxxxx
stopped)*2)
Read status of gas cylinder 2 bytes / hexadecimal
2140
changeover switch for CO2*3) value in format xx
Read status of gas cylinder 2 bytes / hexadecimal
2141
changeover switch for O2*3) value in format xx
Up to 241 bytes / hexadecimal
Read error memory (current
2300 value, format see
errors)*4)
corresponding chapter
Read error memory (prior
errors)*4)
Query (start) of the data stored
in the data logger*5)
Query (start) of the further data
stored in the data logger*6)
(Repeated) query of the most
recent data logger query*7)
Read data logger write cycle in 8 bytes / decimal value in
2410
hours:minutes:seconds format xx:xx:xx
*1) See table with notes on disinfection and autostart run status
*2) 2 bytes per level each
*3) Bottle A active (0x01), bottle B active (0x02), bottle A pressure OK (0x10),
bottle B pressure OK (0x20)
*4) For more information on the error memory, see chapter 13.5.
*5) Set reading pointer to first entry, read up to 7 entries max.
*6) Send the next 7 entries. Set reading pointer automatically to next new entry,
read up to 7 entries max.
*7) Send entries of last telegram again. Can be used after communication error
has occurred.
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Note on *3) disinfection and autostart run status:
Bit Disinfection auto-start

0x00 contra-con not active auto-start not active
0x01 Initialization Initialization
0x02 Await door opening time Await door opening time
0x03 Await door closing Await door closing
0x04 Start Start
0x05 Heat Heat
Perform counter-voltage
0x06 Hold
adjustment
0x07 Condensation Waiting period 1
0x08 Cool-down Set tolerance band
0x09 Dry Establish stable humidity
Perform counter-voltage
0x0A Await release
adjustment
0x0B Stop Waiting period 2
0x0C - Determine offset
0x0D - Read offset, check
0x0E - Release
0x0F - Stop
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13.5 Error memory structure
The error memory contains 22 error messages. A query is responded with

11 data sets with a colon as separator and can be queried using the
following command:
Query: ?:2300:00::cc<CR>
Reading the last 11 error memory entries.
Query: ?:2301:00::cc<CR>
Reading the first 11 error memory entries.
Error memory data set structure These data sets consist of 11 byte and are encrypted in 21 ASCII characters
prior to data transmission. Example: The byte 0x23 is converted into the
ASCII characters 0x32 (‚2’) and 0x33 (‚3’).
– Byte 1 consists of 1 characters,
– Bytes 2 - 11 consist of 2 characters.
Therefore, a response consists of 1+(10 x 2) = 21 data bytes plus
separator.
A data set always delivers the date, the time, the faulty control loop, the
device status, and the error message.
Example of a response:
First data set: !:2300:fb:10b01060f372280000002:

(with 21 bytes)
Second data set: 20b01060f38100001... ...:80

(beginning of the second data set after 21 bytes
of the first data set and of the separator [1 byte])
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13.5.1 Error memory data set structure scheme:
00 0b 01 06 0f 37 22 8000 0002
Faulty control loop type
0x00 = Error in temperature

control loop 00
Date / Day
0x0b = 11th day of the month 0b
Date / Month
0x01 = January 01
Date / Year
0x06 = 2006 06
Time / Hours
0x0f = 15 hrs. 0f
Time / Minutes
0x37 = 55 minutes 37
Time / Seconds
0x22 = 34 seconds 22
Device status
0x8000 = Device error
existing 8000
Error message
0x0002 = Value above set
value upper limit 0002
Therefore, the following information is transmitted in this data set:

• Created on 1 January 2006 at 15:55:34 hrs.
• A device error has occurred and the set temperature is too high.
13.5.2 Overview of the possible error messages in hex coding

Hex-coded error message
Hex code Description / Type

0x00 Temperature control loop
0x01 CO2 control loop
0x02 O2 control loop
0x07 Water level
0x08 General device status
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13.5.3 Overview of the possible error messages in bit coding

Bit-coded error messages for General device status, control loop for temperature and CO2:
temperature and CO2
Bit General device status

0x0002 Device door open too long
0x0004 Display fails to communicate
0x0008 Mainboad parameter implausible (EEPROM faulty)
0x0010 Data logger faulty (device remains operative)
0x0020 Error in disinfection / contraCon
0x0040 No power supply during contraCon
0x0080 Error in autostart
0x0100 Safety circuit activated
0x0200 Bottle turning device fails to communicate
0x2000 Autostart active (info)
0x4000 Disinfection active (info)
0x8000 Device error has occurred (info)
Bit Temperature control loop error status

0x0001 Sensor breakage
0x0002 Value above set value upper limit
0x0004 Value below set value lower limit
0x0008 Set value implausible
0x0010 Calibration value too high / too low
0x0020 Communication error (to sensor)
0x0040 Communication error (to LM75)
0x0100 Measuring cell parameter implausible (EEPROM faulty)
Bit CO2 control loop error status

0x0010 Calibration value too high / too low
0x0040 Communication error (to cylinder changeover switch)
0x0080 No gas, cylinders A and B empty
0x0100 Measuring cell parameter implausible (EEPROM faulty)
0x0200 Cylinder A empty
0x0400 Cylinder B empty
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Control loop for O2 and water level:

Bit-coded error messages for
O2 and water level
Bit CO2 control loop error status
0x0040 Gas cylinder changeover switch fails to communicate
0x0080 No gas, cylinders A and B empty
0x0200 Gas cylinder A empty
0x0400 Gas cylinder B empty
Bit Water level error status

0x0001 No water
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13.6 Data logger structure
The data logger can store up to 10,000 events. Depending on the setting for
the logging cycle (in sections of seconds), e.g. for a value of 60 s (default
value), the events of about 5 days can be stored.
The data logger stores the following information:
• important user actions, system events, and error messages,
• measured data of the three control loops during incubation operation.
The data logger can be queried using the following commands:

Query: ?:2400:00::cc<CR>
Setting the data logger reading pointer to the
oldest entry and output of the first data sets.
Query: ?:2401:00::cc<CR>
Output of the following data sets,
the reading pointer automatically moves gradually from
the older entries to the current entries.
Query: ?:2402:00::cc<CR>
Repeated output of the most recently read data,
this command does not move the reading pointer. This
command can be used to avoid data loss
after a communication error has occurred.
Data logger data set structure Each query command is responded with up to 7 data sets that follow one
another
without a separator. These data sets consist of
16 bytes and are encrypted into 32 ASCII characters prior to transmission.
For example, the byte 0x23 is converted into the ASCII characters:
0x32 (‚2’) and 0x33 (‚3’).
Therefore, a response consists of up to 7 X 16 = 112 bytes, i.e. 224 ASCII
characters.
A data set always delivers the date and the time (without seconds), the
device status and the type of data logger entry (byte 0-7 or ASCII character
0-15).
Also, depending on the entry, the current actual values or set values of the
control loops or other parameters can be entered (byte 8-15 or ASCII
character 16-31).
Example of a response:
First data set !:2400:e0:010b01060f3700000177002800d4000011

(consisting of 32 byte ASCII characters)
Second data set 0b01060f3800000172003200d20352... ...:80
(beginning of the second data set after 32 bytes
of the first data set)
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13.6.1 Data logger data set structure scheme:
01 0b 01 06 0f 37 0000 0177 0028 00d4 0000
Data logger entry type
0x01 = Standard entry

that is stored every
60 s 01
Date / Day
0x0b = 11th day of the
0b
month
Date / Month
0x01 = January 01
Date / Year
0x06 = 2006 06
Time / Hours
0x0f = 15 hrs. 0f
Time / Minutes
0x37 = 55 minutes 37
Device status
0000
0x0000 = Everything
OK
Type-specific, here:
current temperature in
1/10°Celsius
0177
0x0177 = 37.5°C
current CO2 content in
1/10%
0028
0x0028 = 4.0% CO2
current O2 content in
1/10%
00d4
0x00d4 = 21.2% O2
Reserved for rh in
1/10% always
0000
0x0000 = 0%
Therefore, the following information is transmitted in this data set:

• Created on 1 January 2006 at 15:55 hrs.
• The device status reports no particularities,
• the temperature is 37.5°C,
• gas concentration 4.0% CO2, 21.2% O2.
Example of a code:
For an example of a code, please refer to the end of this
chapter.
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13.6.2 Overview of possible event entries in bit coding

Bit-coded event entries Overview of event entries, Part I:
Code Event Special information (byte 8-15)

Set values from all control
Current values for temperature,
0x01 loops (periodically in minute
CO2, O2, and rh
cycles)
Set value change (at beginning Set value for temperature, CO2,
0x02
of new section) O2, and rh
Set value for temperature, CO2,

0x10 Change temperature set value
O2, and rh

0x11 Change CO2 set value
O2, and rh

0x12 Change O2 set value
O2, and rh
Status / error registration for

0x20 New temperature error
temperature, CO2, O2, and rh

0x21 New CO2 error

0x22 New O2 error

0x2F New system error

0x30 Power reset
O2, and rh
Current actual values for

0x31 Door open

0x32 Door closed
Calibration level (2 bytes), old

Customer-specific
0x40 temperature, new temperature
temperature calibration
(2 bytes each)
Customer-specific CO2
0x41 CO2 value, new CO2 value (2
calibration
bytes each)
Customer-specific O2
0x42 O2 value, new O2 value (2 bytes
calibration
each)
0x50 Start auto-start
auto-start successfully Current actual values for

0x51
completed temperature, CO2, O2, and rh
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Bit-coded event entries Overview of event entries, Part II:
Code Event Special information (byte 8-15)

0x52 auto-start completed with error

0x53 auto-start stopped manually

0x60 Start contra-con
contra-con successfully Current actual values for

0x61
completed temperature, CO2, O2, and rh
contra-con completed with Status / error registration for

0x62
error temperature, CO2, O2, and rh

0x63 contra-con stopped manually
Status gas monitoring (2

0x70 Gas monitor cylinder A empty
bytes), 4 bytes empty
Status gas monitoring (2

0x71 Gas monitor cylinder B empty
bytes), 4 Bytes empty
Gas monitor manual Status gas monitoring (2

0x72
changeover bytes), 4 Bytes empty

0x90 Start low humidity

0x91 Stop low humidity
Drive roller speed status (2

0x92 Start bottle turning device
bytes each)
Drive roller speed status (2

0x93 Stop bottle turning device
bytes each)

0xe0 Delete data logger
No information, not even on

0xff Last entry of data logger
date, time, and status
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13.7 Examples of data logger codes
Data logger entry An entry in the data logger is 16 bytes large and has the following structure:
1. byte: indicates the event (e.g. door open 0x31, measured value entry
0x01)
2. byte: day of entry
3. byte: month
4. byte: year
5. byte: hour
6. byte: minute
7. and 8. byte: device status
9. to 16. byte: various data on the event
13.7.1 Functions for data logger queries

Functions The following code example for reading the data logger uses six functions:
• ahex
// converts the received ASCII character into a hexadecimal number,
• send_telegramm
// sends a query to the data logger,
• get_telegramm
// receives a response data the data logger,
• time_2_str
// uses a hexadecimal value to create ASCII characters in time format,
• num_2_string
// uses hexadecimal values to create ASCII characters to be entered into
a file,
• read_datalogger
// edits the received data and writes them into a file.
13.7.2 Example of a code for a a data logger query

Creating a hexadecimal number char ahex (char a)
char ahex(char a)
{
char i;
char hexa[16]="0123456789abcdef";
for (i = 0; i < 16; i++)

if (a == hexa[i])
return (i);
return 0;
}
Sending a telegram send_telegramm

void send_telegramm(char *p)
{
char string [15];
unsigned char bcc = 0xFF;
char i;
// copy telegram together

strncpy (&string[0], "?:xxxx:00::00\r", 14);
// insert 4-digit address
strncpy (&string[2], p, 4);
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// calculate checksum: inverted XOR of all bytes

// without checksum and <CR>
for (i = 0; i < 11; i ++)
bcc = (bcc^string[i]);
// copy checksum
string[11] = hexa(bcc/16);
string[12] = hexa(bcc%16);
// send telegram
ComWrt (COM_NR, string, 14);
return;
}
Receiving a response telegram get_telegramm

int get_telegramm(char *p)
{
int reading_count = 0;
// reading the telegram character by character
do
ComRd(COM_NR, &p[reading_count], 1);
// until reception of <CR>
while ((p[reading_count++] != '\r'));
// return = number of received characters
return (reading_count);
}
Creating a time format in ASCII time_2_str

char time_2_str (int z, char * b)
{
char i;
// output two numerals
for (i = 1; i >= 0; i--){
// calculate value
b[i] = z%10+0x30;
// reduce default value
z = z/10;
}
return (2);
}
Hexadecimal values in ASCII num_2_string

characters char num_2_str (int z, char * b)
{
// number with one decimal place
char a[12];
char i, l;
int rest = 0;
l = 0;
// Negative number?
if (z < 0) {
// set algebraic sign
b[0] = '-';
l = 1;
// convert value
z = 0xffffffff-z+1;
}
// store decimal place
rest = z % 10;
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// cut off decimal place

z = z / 10;
// calculate and copy number before decimal separator
for (i = 0; i < 12; i++){
// calculate value
a[i] = z%10+0x30;
// reduce default value
z = z/10;
// Number copied completely?
if (z == 0) break;
}
for ( ; i >= 0; i--)b[l++] = a[i];
// calculate and copy number after decimal separator
b[l++] = ',';
// calculate value
b[l++] = rest%10+0x30;
return (l);
}
Writing data in a file read_datalogger

int read_datalogger ()
{
#define SIZE_DATA2 16
#define EVENT_STATUS 0x01
unsigned char buffer[300], string [300];
unsigned char zahlenstring [150],datestring ,timestring ;
unsigned char excelstring [150];
unsigned char len, h,i;
unsigned int read_count,status;
#define EVENT_DATA.END 0xFF
char data;
int GetTele = 0
GetError = 0,
// writing the title line in the file
WriteFile (FileHandle, "Date;Time;Comment;Temp Act.;CO2 Act.;O2
Act.;rH Act.;Temp Set;CO2 Set;O2 Set;rH Set;\n", 85);
// infinite loop
while (1)
{
// set data logger to beginning and read
if (!GetTele){
send_telegramm ("2400");
}
else{
// read additional data sets
}
len = get_telegramm (buffer);
// no telegram received
if (!len) {
GetError ++;
// requery
len = get_telegramm (buffer);
// again, no telegram received
if (!len) return 1;
}
// increase telegram counter
GetTele ++;
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// length of sent payload

len = (ahex(buffer[7]) * 0x10 + ahex(buffer[8])) / 2;
// converting ASCII string into usable numeric string
for (i = 0; i < (len); i++)
zahlenstring [i] = (ahex(buffer[10 + (2*i)]) * 0x10 +
ahex(buffer[11 + (2*i)]));
// calculation of the sent data packages
data = ((len) / SIZE_DATA2);
// evaluation of all data packages
for (i = 0; i < data; i++)9{
len = 0;
// write time and date into file
len += time_2_str (zahlenstring[1+i*SIZE_DATA2],
&excelstring[len]);
excelstring[len ++] = '.';
&excelstring[len]);
excelstring[len ++] = '.';
&excelstring[len]);
excelstring[len ++] = ';';
&excelstring[len]);
excelstring[len ++] = ':';
&excelstring[len]);
excelstring[len ++] = ':';
len += time_2_str (0, &excelstring[len]);
switch (zahlenstring[i*SIZE_DATA2]){
case EVENT_STATUS:
// check cyclic entries for device errors
status = zahlenstring[6+i*SIZE_DATA2]*0x100+
zahlenstring[7+i*SIZE_DATA2];
if (status & INFO_ERROR){
str_cpy (&excelstring[len], "Error active;", 13);
len += 13;
}
else{
// query all device errors (see chap. 13.6.2‚ pg. 139)
if (status & DOOR_LONG){
str_cpy (&excelstring[len], "Door open too long;",
19);
len += 19;
else {
if (status & DOOR_OPEN){
str_cpy (&excelstring[len], "Door open;", 10);
len += 10;
}
}
// query remaining device errors now
// ·
// ·
// ·
// ·
// ·
// and finally query cyclic nominal values entries without device
// errors
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else{
str_cpy (&excelstring[len], "ok;", 3);
len += 3;
}
}
// copy nominal values from numerical string to Excel string
len += num_2_str ((zahlenstring[8+i*SIZE_DATA2]*0x100+
zahlenstring[9+i*SIZE_DATA2]), &excelstring[len]);
// enter set values from here
len += num_2_str (SollTemp, &excelstring[len]);
len += num_2_str (SollCO2, &excelstring[len]);
len += num_2_str (SollO2, &excelstring[len]);
len += num_2_str (SollrH, &excelstring[len]);
excelstring[len] = '\n';
len += 1;
WriteFile (FileHandle, excelstring, len);
break;
// query the remaining events from here
case EVENT_FORMAT_DATALOG:
WriteFile (FileHandle, "Data logger erased;\n",20);
break;
case EVENT_POWER_ON:
// update of set values
SollTemp = zahlenstring[8+i*SIZE_DATA2]*0x100+
SollCO2 = zahlenstring[10+i*SIZE_DATA2]*0x100+
SollO2 = zahlenstring[12+i*SIZE_DATA2]*0x100+
SollrH = zahlenstring[14+i*SIZE_DATA2]*0x100+
WriteFile (FileHandle, "Power on;\n", 10);
break;
case..
// query all events here (see chap. 13.6.2‚ pg. 139)
// cancel 0xFF indicates the end of the data logger
case 0xFF:
WriteFile (FileHandle, "End;\n",5);
}
}
return 0;
}
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13.8 Program Heracell 150i & 240i
User surface for data communication The program provides a user surface (only with English menu designation)
for handling the data communication between a device and a connected
PC.
This program is used for:

• Reading and archiving error messages (error logger). The data sets are
stored in the meta format *.CSV.
• reading and archiving event entries (data logger). The data sets are
stored in the meta format *.CSV.
• Creating a service file (servicefile) to be sent to the Technical Service of
Thermo Fisher Scientific. The information of the service file is very
useful for systematic troubleshooting. The data sets are stored in the
proprietary format *.SRF.
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13.8.1 Installing the Heracell 150i & 240i

Installation routine 1. Starting the installation routine:
> On the data CD in the subdirectory PROGRAMS, double-click on the
file SETUP.EXE to open this file.
2. Locate the installation directory for the program.
3. In the sequence of the given installation steps:

– confirm the licence agreement,
– confirm the extent of installation,
– after the message on the completeness of the installation has been
displayed, close the installation surface and restart the computer.
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13.8.2 Operating the Heracell 150i & 240i
User menu structure:

The user surface is divided into two main menus:
• MAIN with two functional elements:
– Program version output FIRMWARE VERSION
– Switch for exiting the program: QUIT
Submenus • GENERAL with the submenus:

– PRESETTING for setting the transmission speed and for selecting
the serial port,
– TEST COM for testing the communication connection between PC
and incubator,
– DATE & TIME for adjusting date and time to the desired time zone.
– ERROR LOGGER for reading the error messages,
– DATA LOGGER for reading the event entries,
– SERVICEFILE for reading error information and for creating a
service file,
– PASSWORD to prevent access to the device parameters of the
incubator.
50115191 B / 11.2010 148

®
Function of the user menu:

PRESETTING
Presetting The submenu PRESETTING is used to set the transmission speed and to
select the serial port.
1. Select a transmission speed within the range of 9,600 to 115,200 baud.

2. Select the serial port of the PC. If the USB driver is installed, the
(virtual) COM port assigned to the USB port can be selected (see chap.
13.1.2‚ pg. 125).
3. Accept the settings:
> Press the CHANGE button.
4. Save the settings (in an INI file):
> Press the SAVE TO FILE button.
Transmission speeds:
The transmission speed settings in the user menu PRESETTING
and of the device must be identical!
50115191 B / 11.2010 149

®
TEST COM
Test Com The submenu TEST COM is used for testing the communication
connection with the settungs defined in the submenu PRESETTING.
1. Example of a query for the currently measurable temperature values of

the incubator:
> Query: ? (default, not changeable)
> Address: 2010 (temperature value address: Set value, actual value,
reference value)
2. Send the query to the incubator:
3. Press the SEND button.
> If the incubator returns a response string, the communication
connection to the incubator has been established.
> If a connection cannot be established, an error dialog is displayed:
4. Exit the error dialog:

> Press the OK button.
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DATE & TIME

Date & Time The submenu DATE & TIME is used for adjusting date and time to the
desired time zone.
1. Data in the two text boxes must be entered in the format DD.MM.YY
(day, month, year).
2. Accept the input data:
> Press the SET button.
ERROR LOGGER
Error Logger The submenu ERROR LOGGER is used for reading the error messages into
the text box of the user surface.
The data sets can be stored in the metaformat *.CSV.
50115191 B / 11.2010 151

®
• Saving data sets as files:

> Press the SAVE TO FILE button.
DATA LOGGER
Data Logger The submenu DATA LOGGER is used for reading the event entries into the
text box of the user surface.
The data sets can be stored in the metaformat *.CSV.

1. Read the data sets:
> Press the READ button.
2. The progress of the data transmission is indicated in the three text
boxes:
> AMOUNT DATA: Total number of transmitted data sets.
> AMOUNT TELEGRAMS: Therein the number of transmitted event
entries.
> AMOUNT ERRORS: Therein the number of transmitted error
messages.
Duration of the data transmission:

As the data logger can contain up to 10,000 data sets, the data
transmission to a PC may take some time.
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SERVICEFILE
Servicefile The submenu SERVICEFILE is used for reading error informations and for
creating a service file from it, saved with the proprietary extension *srf. The
service file is transmitted to the Technical Service of Thermo Fisher Scien-
tific for fault analysis.
1. Create the service file :

> Press the CREATE SERVICEFILE button.
> Define file name and saving directory in the Windows dialog box.
2. Start saving process:
> Press OK button.
Duration of the procedure:

The compilation of device informations and the creation of the
service file may take some time.
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PASSWORD
Password The submenu PASSWORD can only be accessed by the service personnel
of Thermo Fisher Scientific.
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14 Device log
Please list carried out works here:

Device type: Part number:
Serial number: Service number:
Location: Operator's note:
Work carried out Notes Date Signature
50115191 B / 11.2010 155

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Operating Instructions

Thermo Electron LED GmbH

Thermo Electron LED GmbH is a subsidiary company of:

2 Delivery of the device 18

3 Installation of the device 22

4 Description of the device 26

4.10.6 Rear panel openings .................................................................................... 46

7 Handling and control (iCan™ touchscreen controller) 65

7.13.1 Response to an error message event ......................................................... 95

9 Cleaning and disinfection 101

12 Technical data 121

13 Annex: Data communication 125

13.5.3 Overview of the possible error messages in bit coding........................... 135

14 Device log 155

Fig. 1: Device dimensions.................................................................................... 23

Fig. 50: Logging cycle setting ................................................................................ 83

Overview of the Thermo Fisher international sales organisations

Europe, Middle East, Africa Postal address Germany:

Enquiries from Germany:

Enquiries from Europe, Middle East, and Africa:

America Postal address USA:

Enquiries from North America:

Enquiries from Latin America:

Enquiries from Asia Pacific:

1.1 Identification of the device and of the documentation

Identification of the device

Allocation of product documentation

Certifications and quality audit:

1.2 Instruction of the operating personnel

These operating instructions describe the CO2 incubator HERAcell® 150i /

1.3 Applicability of the instructions

1.5 Explanation of safety information and symbols

1.5.1 Safety information and symbols used in the operating

WARNING: Personal injuries

Indicates a hazardous situation which, if not avoided, could

CAUTION: Personal injuries

Indicates a hazardous situation which, if not avoided, could

NOTICE: Property damage

NOTE: Useful information

Is used for applicational hints and useful information.

1.5.2 Additional symbols for safety informations:

Wear safety gloves!

Wear safety goggles!

1.5.3 Symbols on the device

CE conformity mark: confirms conformity according to EU Guidelines

H VDE test mark

Mark of conformity USA/Canada

Observe operating instructions!

1.6 Intended purpose of the device

1.6.1 Correct use

1.6.2 Incorrect use

1.7 Standards and directives

The device complies with the following standards and guidelines:

1.8 Safety notes on gases

Instruction of the personnel:

These instructions must be repeated at appropriate intervals and must

1.8.1 Safety notes on carbon dioxide (CO2)

1.8.2 Safety notes on oxygen (O 2)

1.8.3 Safety notes on nitrogen (N 2)