“Steve Kalkanis is a good example of what a modern Departmental Chairman should be. Philip Hessburg”
Steven Kalkanis MD
Detroit, Michigan, United States
3K followers
500+ connections
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Thank you for your kind words and for joining us at this milestone event John Kowal and Arthur Kaindl. We deeply value our partnership and…
Thank you for your kind words and for joining us at this milestone event John Kowal and Arthur Kaindl. We deeply value our partnership and…
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Father-son interventional cardiologist duo Dr. William O'Neill and Dr. Brian O'Neill teamed up to perform Michigan's 1st artificial tricuspid heart…
Father-son interventional cardiologist duo Dr. William O'Neill and Dr. Brian O'Neill teamed up to perform Michigan's 1st artificial tricuspid heart…
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Publications
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Raman spectroscopy to distinguish grey matter, necrosis, and glioblastoma multiforme in frozen tissue sections
Journal of neuro-oncology
The need exists for a highly accurate, efficient and inexpensive tool to distinguish normal brain tissue from glioblastoma multiforme (GBM) and necrosis boundaries rapidly, in real-time, in the operating room. Raman spectroscopy provides a unique biochemical signature of a tissue type, with the potential to provide intraoperative identification of tumor and necrosis boundaries. We aimed to develop a database of Raman spectra from normal brain, GBM, and necrosis, and a methodology for…
The need exists for a highly accurate, efficient and inexpensive tool to distinguish normal brain tissue from glioblastoma multiforme (GBM) and necrosis boundaries rapidly, in real-time, in the operating room. Raman spectroscopy provides a unique biochemical signature of a tissue type, with the potential to provide intraoperative identification of tumor and necrosis boundaries. We aimed to develop a database of Raman spectra from normal brain, GBM, and necrosis, and a methodology for distinguishing these pathologies. Raman spectroscopy was used to measure 95 regions from 40 frozen tissue sections using 785 nm excitation wavelength. Review of adjacent hematoxylin and eosin sections confirmed histology of each region. Three regions each of normal grey matter, necrosis, and GBM were selected as a training set. Ten regions were selected as a validation set, with a secondary validation set of tissue regions containing freeze artifact. Grey matter contained higher lipid (1061, 1081 cm(-1)) content, whereas necrosis revealed increased protein and nucleic acid content (1003, 1206, 1239, 1255-1266, 1552 cm(-1)). GBM fell between these two extremes. Discriminant function analysis showed 99.6, 97.8, and 77.5 % accuracy in distinguishing tissue types in the training, validation, and validation with freeze artifact datasets, respectively. Decreased classification in the freeze artifact group was due to tissue preparation damage. This study shows the potential of Raman spectroscopy to accurately identify normal brain, necrosis, and GBM as a tool to augment pathologic diagnosis. Future work will develop mapped images of diffuse glioma and neoplastic margins toward development of an intraoperative surgical tool.
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👏 We were honored to have Bob Riney, President & CEO of Henry Ford Health, join us at the Detroit Lions Thanksgiving Day game in support of Game On…
👏 We were honored to have Bob Riney, President & CEO of Henry Ford Health, join us at the Detroit Lions Thanksgiving Day game in support of Game On…
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Henry Ford Providence Southfield Hospital has been recognized as one of America’s 100 Best Hospitals for Cardiac Care™️ by Healthgrades. This…
Henry Ford Providence Southfield Hospital has been recognized as one of America’s 100 Best Hospitals for Cardiac Care™️ by Healthgrades. This…
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We’re thrilled to share that our President & CEO Robert Riney has been named one of Modern Healthcare’s 100 Most Influential People in Healthcare for…
We’re thrilled to share that our President & CEO Robert Riney has been named one of Modern Healthcare’s 100 Most Influential People in Healthcare for…
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Incredible tour of the White House with current White House Fellow and neurosurgery rising star Nalini Tata. Amazing experience! And the White…
Incredible tour of the White House with current White House Fellow and neurosurgery rising star Nalini Tata. Amazing experience! And the White…
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Thank you to my colleagues for the opportunity to serve as Chair of the Advisory Council for Neurological Surgery at the American College of…
Thank you to my colleagues for the opportunity to serve as Chair of the Advisory Council for Neurological Surgery at the American College of…
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Excited to share that I have been selected as a Distinguished Fellow for the American Academy of Physician Associates! I’m looking forward to serving…
Excited to share that I have been selected as a Distinguished Fellow for the American Academy of Physician Associates! I’m looking forward to serving…
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Are you curious to know if emergency medicine is the right specialty for you? Our emergency medicine physician Dr. Sam Mossallam is a featured doctor…
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