Jo Koy Jokes About Robert De Niro's Growing Family in 2024 Golden Globes Monologue: 'How'd You Get Her Pregnant?'

Jo Koy kicked off the 81st annual Golden Globe Awards with a bang.

The comedian gave a shoutout to some of the A-listers in the audience, including Ali Wong and her smash hit Beef, Kevin Costner and Meryl Streep while also joking that he "lied" about having seen all the TV shows and movies nominated at this year's show.

Koy, 52, got the crowd going as he addressed Robert De Niro, who he said he's a big "fan" of before asking: "How'd you get her pregnant at 80?"

"CGI?" he asked, referring to De Niro's newborn daughter, whom he and his girlfriend Tiffany Chen welcomed in April. Koy then turned away from the actor and asked the audience, feigning shyness, "Is he looking? Is he looking?"

On a similar topic, he called out Barry Keoghan, who lowered his head when the host singled him out.

"Where's your penis seated? Down front?" Koy said, shouting out a full-frontal nude scene that concluded Emerald Fennell's Saltburn. "That was the real star of the show."

He then said that something was "missing" in Barbie, compared to what was "dancing" in Saltburn, before landing a jab at Bradley Cooper and his prosthetic nose in Maestro.

During his monologue, Koy also made some jokes about another blockbuster hit from 2023, as he quipped that Oppenheimer "needed another hour," before saying that his resolution for 2024 was to finally finish the Christopher Nolan film.

"Oppenheimer answered my question that had been on my mind for years – yes, scientists do get laid. As long as they look like Cillian Murphy."

During Variety and Entertainment Tonight's Golden Globes digital pre-show, Koy said that the hosting gig was his chance to "show what I can do."

"I wanna bring my flavor, my style," he said of his approach to the show, which he called a chance to "celebrate" Hollywood after the months-long strikes.

He also said that he was "in complete awe" when he was asked to host. It was his "holiday present," he joked.

The comedian was announced as this year’s host in December, and called the role “extra special" in a statement.

“This is that moment where I get to make my Filipino family proud,” Koy said. 

“We are thrilled to have Jo host the 81st annual Golden Globe Awards and bring his infectious energy and relatable humor to kick off Hollywood’s award season. We can’t wait to see what he has in store for the stars in the room and a global audience,” said Golden Globes President Helen Hoehne. “We know Jo is bringing his A-game.”

See PEOPLE's full coverage of the 81st annual Golden Globes as they're broadcasting live from The Beverly Hilton in Los Angeles on CBS and Paramount+.

Last year’s show did not feature a host, as its former broadcast partner NBC declined to air that year's ceremony in response to the scandal.  

Other recent hosts include Tina Fey and Amy Poehler, who cohosted the Globes for the fourth time in 2021, and Ricky Gervais, who also made his fourth hosting appearance at 2020's ceremony.