atSpiro

🎵 All I want for Christmas is 🎵 more time and a simpler way to run my cell cultures. We can't grant all your wishes, but we can fulfill one: making your lab work easier and more efficient in the new year! The ShakeReactor can be your lab's secret weapon, turning traditional shake flasks into fully controlled bioreactors with automated feeding, real-time monitoring, and yield improvements. Treat yourself in 2025 upgrading your lab with faster and simpler cultivations.

Running flask cultivations? Learn how the #ShakeReactor transforms flasks into bioreactors, enabling continuous monitoring and automatic control. Our CEO, Toke Faurby, will be presenting how the ShakeReactor supports material production, strain screening, and process development for cell culture and microbial applications. 📅 Wednesday, December 11th 🕔 17:05 CET / 11:05 AM ET / 8:05 AM PT Register here: https://lnkd.in/gbra6cHn Agenda: https://lnkd.in/gZKZ3-sb

Integrating new tools into the lab can often feel overwhelming. The ShakeReactor breaks this norm, it’s simple enough to be mastered in an afternoon. When we designed the ShakeReactor, our goal was to reduce the time required to set up fed-batch cultivations while increasing throughput. This was achieved, among other things, by simplifying the setup process and equipment, making 80% of traditional benchtop bioreactor setup redundant and reducing the amount of required components by 70%, compared to traditional benchtop bioreactors. An added benefit of this approach became that learning it is surprisingly easy. A traditional benchtop bioreactor protocol looks like this: - Preparing the Bioreactor (e.g., media addition, sensor mounting): ~5 steps - Autoclaving (vessel and components): ~8 steps - Connecting Components (e.g., sensors, tubing, and heating): ~12 steps - Calibration (e.g., pH, DO sensors): ~10 steps - Process Setup (e.g., parameter input, PID tuning): ~6 steps - Experiment Start and Monitoring: ~5 steps Total – 56 steps In contrast the ShakeReactor requires far fewer steps: - Preparation (e.g., gathering components, sterilization where applicable): ~2 steps - ShakeReactor Assembly (e.g., inserting sensors, attaching the device): ~6 steps - Experiment Start and Monitoring: ~3 steps Total – 11 steps ShakeReactor is designed to be easy to learn, it consists of only 5 parts parts that come sterilized, calibrated and ready to use: - ShakeReactor - Shake flask (500–1000 mL) - Pre-sterilized and calibrated single-use sensor (pH & DO) - Pre-sterilized liquid containers (up to 3 per unit) - Battery for ShakeReactor We’d have liked to include all the parts in a benchtop bioreactor in this post, but the list is simply too long… If you're interested in the atSpiro protocol, reach out to us through our website.

Exploring the Future of Cell Cultivation with the ShakeReactor! This week, our CEO Toke Faurby, showcased the ShakeReactor to key biotech leaders in southern Germany, including Sandoz, Fraunhofer IPA, Rentschler Biopharma, and MSD Animal Health. We are excited to continue collaborating with the dynamic #biotech communities in southern Germany and Switzerland. The #ShakeReactor transforms standard shake flasks into #bioreactors with #automated feeding, pH control, and real-time monitoring—streamlining #cell #cultivation and #bioprocess development. We were thrilled to discuss the diverse applications of the ShakeReactor and how to overcome challenges in the lab with top scientists and industry experts. A big thank you for a great week! Dan Ma, Lotte Lundø Nørgaard, Yilmaz Niyaz, Romke-Jan van Dijk, Denis Kartavyi, Andreas Traube, Thilo Grob, Dr. Susanne Gulden, Christoph Neubrand, Vera Koppenburg, Felix Dulich, Marco Gassen, Franziska Lausenmeyer, Christiane Bardroff, Innovation Centre Denmark - Munich, Sandoz in Deutschland

Are your shake flask cultures getting enough oxygen? When working with mammalian shake flask cultures, do you find yourself asking questions like: - What impact does a 15 RPM increase in shaking have? - How does a 40 mL change in media volume affect the culture? - When are all the substrates used up? - When did the stationary phase begin? Traditional shake flask setups often lack the ability to collect critical data, making it difficult to address fundamental questions about cell health and metabolism. This leaves you guessing when a culture behaves unexpectedly. We have seen that the #ShakeReactor brings transparency to shake flask cultures. By integrating dissolved oxygen monitoring, it provides valuable insights to troubleshoot issues and develop optimized protocols. With continuous oxygen concentration data, you can: - Detect substrate depletion—thanks to the rapid increase in dissolved oxygen when substrates run out. - Identify exact timing of culture phases—from exponential growth to stationary and death phases, based on changes in oxygen consumption. - Understand the effects of media volume adjustments—whether from sampling or feeding, and their impact on oxygen mass transfer. And that's just the beginning! Learn more about how the ShakeReactor can improve your cell culture work. Visit our page or website today!

Flask experiments are slow, tedious, and every time you feed or sample, you risk disturbing and contaminating your cell culture. Why risk it? The ShakeReactor optimizes your workflow with easy, contamination-free sampling and automated feeding. Setup is quick, and there’s no need to constantly monitor your cultures. Upgrade your lab and streamline your workflow to keep both your cells and your team happy. Visit atSpiro.com to learn more. 

The ShakeReactor is designed to improve lab-scale cultivation by simplifying the process. Here’s a closer look at its standout features: 1. Enhanced Performance: Much like a rocket, propelling your cultures to new heights, the ShakeReactor is engineered for FAST set up. It provides precise control over environmental parameters, enabling researchers to achieve superior cell growth and productivity. 2. User-Friendly Design: Just as a 5-piece puzzle can be assembled by anyone, the ShakeReactor is SIMPLE to set up and operate. Its intuitive interface enables rapid experimentation and troubleshooting so scientists can focus on their research without getting overwhelmed by complicated equipment. 3. Streamlined Learning Curve: Similar to a single-colored Rubik's Cube, the ShakeReactor is designed to be EASY to use. Researchers can learn its functionalities in a few hours, leading to faster insights and advancements. 4. Space Efficiency: Much like a astronaut floating in space, the ShakeReactor does not take up a lot of SPACE. It optimizes lab space without sacrificing performance, making it ideal for any research setting. In essence, the ShakeReactor bring simplicity & efficiency to your bioreactor experiments. We want you to not be limited by throughput, such that you can run the experiments you need, and get the outcome you want. Visit our website, or browse through our LinkedIn page to learn more.

The ShakeReactor is designed for ultimate flexibility, adapting seamlessly to a wide range of shakers and shaking incubators, supporting speed up to 300 RPM for 25-mm shakers and 200 RPM for 50-mm shakers. Turn your flasks into bioreactors and: • Automate tedious tasks such as manual feeding • Increase process understanding with online monitoring of pH and dissolved oxygen • Reduce manual handling of flasks by utilizing needle free sampling ports The ShakeReactor with its programmable pumps, monitoring, and online connectivity allows you to turn shake flasks into remote controlled bioreactors, without the complexity of stirred bioreactors. Setting up the ShakeReactor can be done in 10 minutes and its user-friendly interface can be used by anyone. Visit our page to learn more. #biotechnology #bioreactor #shakenfermentation #labautomation #research #processoptimization #cellculture

👀 What’s really happening inside your shake flask? 👀 In most labs, shake flasks and microtiter plate cultures are essential for testing various conditions and strains. However, they often function as “black boxes” where only sporadic offline samples offer a glimpse into what’s actually happening. 𝗽𝗛 is one of the most critical process parameters to monitor and control, yet in traditional flasks, it’s not easily tracked or controlled—leaving us to rely on buffers alone. In the video, you can see continuous data from an E. coli culture grown in M9 medium with 4 g/L of glucose. As the cells grow, dissolved oxygen (DO) drops while pH decreases, indicating the production of acid metabolites. After ~6 hours, the glucose is depleted, causing DO to rise back to 100%, while pH recovers as E. coli begins metabolizing the acid byproducts. Even with the 70 mM phosphate buffer in M9 medium, we observed a ~1 unit pH drop, which could significantly impact experimental outcomes. The rapid metabolic dynamics seen in microbial cultures are nearly impossible to capture through traditional sampling and offline analysis. 𝗧𝗵𝗲 𝗦𝗵𝗮𝗸𝗲𝗥𝗲𝗮𝗰𝘁𝗼𝗿 changes this. By tracking pH and DO continuously, it reveals the hidden dynamics of shake flask cultures. And with its 3 programmable pumps, it enables precise control and feeding, turning your flasks into bioreactors. 🎯 𝗪𝗵𝗲𝗻 𝘆𝗼𝘂 𝗰𝗮𝗻 𝘀𝗲𝗲 𝘄𝗵𝗮𝘁'𝘀 𝗵𝗮𝗽𝗽𝗲𝗻𝗶𝗻𝗴, 𝘆𝗼𝘂 𝗰𝗮𝗻 𝙘𝙤𝙣𝙩𝙧𝙤𝙡 𝗶𝘁. Imagine what this insight could do for your research. #biotech #phmonitoring #labinnovation #microbialcultivation

The ShakeReactor is designed to streamline lab-scale cultivations, turning flasks into bioreactors in just 10 minutes. Fast, simple, and efficient just like flasks, but as smart as a bioreactor. Ready to optimize your lab workflows? Contact us at atSpiro.com