P 900 Pump

GE Healthcare
ÄKTAdesign
Pump P-900
User Manual
ÄKTA™
Important user information
All users must read this entire manual to fully understand
the safe use of the component.
WARNING!
The WARNING! sign highlights instructions that
must be followed to avoid personal injury. It is
important not to proceed until all stated conditions
are met and clearly understood.
CAUTION!
The Caution! sign highlights instructions that must
be followed to avoid damage to the product or
other equipment. It is important not to proceed
until all stated conditions are met and clearly
understood.
Note
The Note sign is used to indicate information
important for trouble-free and optimal use of the
product.
CE Certifying
This product meets the requirements of applicable CE-
directives. A copy of the corresponding Declaration of
Conformity is available on request.
The CE symbol and corresponding declaration of
conformity, is valid for the instrument when it is:
– used as a stand-alone unit, or
– connected to other CE-marked GE Healthcare
instruments, or
– connected to other products recommended or
described in this manual, and
– used in the same state as it was delivered from
GE Healthcare except for alterations described in this
manual.
Recycling
This symobl indicates that the waste of electrical
and electronic equipment must not be disposed
as unsorted municipal waste and must be
collected separately. Please contact an authorized
representative of the manufacturer for information
concerning the decommissioning of equipment.
Contents
Contents
1 Introduction
1.1 General .....................................................................................................7
1.1.1 Accessories ...........................................................................................................8
1.2 Safety .....................................................................................................................9
2 Installation
2.1 Unpacking ............................................................................................ 11
2.2 General precautions........................................................................ 11
2.3 Connecting electrical signal cables .........................................11
2.3.1 Connecting to chart recorder (if used) .................................................. 12
2.4 Connecting to communication link ..........................................12
2.5 Connecting to supply voltage ....................................................12
2.6 Installing the piston seal rinsing system ...............................13
2.7 Connecting the inlet tubing .........................................................14
2.8 Purging the pump ............................................................................ 14
2.9 Running-in the new pump ...........................................................15
2.9.1 General ................................................................................................................ 15
2.9.2 Running-in ......................................................................................................... 15
2.10 Connecting the pump outlet tubing ........................................16
2.11 Installation of accessories ..........................................................16
3 Operation
3.1 On/off ..................................................................................................... 19
3.2 Menu selection and settings .......................................................19
3.2.1 Menu selection ................................................................................................ 19
3.2.2 Return to main menu ................................................................................... 20
3.2.3 Select value ....................................................................................................... 20
3.3 Main menu overview ......................................................................20
3.4 Starting and stopping the pump ...............................................21
3.4.1 Main operating menu ................................................................................... 21
3.4.2 Mode changing menu .................................................................................. 21
3.4.3 Preparation before starting ....................................................................... 21
3.4.4 Emergency stop .............................................................................................. 22
3.5 Setting the flow rate and starting the pump ......................22
3.6 Setting concentration B.................................................................22
3.7 Running a simple gradient ...........................................................23
3.8 Ending the run and storage ........................................................23
3.9 Changing eluent ................................................................................ 24
3.10 Restart after power failure ...........................................................24
ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE 5

Contents
4 Maintenance
4.1 Periodic maintenance ....................................................................25
4.2 Cleaning-in-place ............................................................................. 25
4.3 General care ....................................................................................... 25
4.3.1 General recommendations for all eluents ........................................... 26
4.3.2 Additional recommendations for aqueous eluents ......................... 26
4.4 Replacing the piston seal .............................................................26
4.5 Running-in a new piston seal .....................................................31
4.6 Replacing a damaged piston ......................................................32
4.7 Cleaning the inlet and outlet check valves ..........................32
4.7.1 Cleaning after removal ................................................................................ 32
5 Trouble shooting
5.1 General .................................................................................................. 35
5.2 Faults and actions ............................................................................35
5.3 Error messages .................................................................................. 36
5.4 Checking the pump pressure......................................................37
5.5 Removing trapped air bubbles from the pump .................38
A Description
A.1 Instrument ........................................................................................... 39
A.2 Pump head .......................................................................................... 41
A.3 Pump principle ................................................................................... 43
A.4 Outlet manifold .................................................................................. 43
A.5 Pressure monitoring ........................................................................44
A.6 Using an external chart recorder .............................................44
6 ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE

Contents
B Menus
B.1 Check menu ........................................................................................ 45
B.1.1 Checking piston stroke ................................................................................. 45
B.1.2 Check pump operation time ...................................................................... 45
B.1.3 Check number of piston strokes .............................................................. 45
B.1.4 Check Service Mode ...................................................................................... 45
B.1.5 Calibrate Pressure Offset ............................................................................ 46
B.2 Setup menu ......................................................................................... 46
B.2.1 Set pressure limit ............................................................................................ 46
B.2.2 Set pressure full scale on recorder ......................................................... 46
B.2.3 Set pressure unit ............................................................................................. 46
B.2.4 Set gradient base ........................................................................................... 46
B.2.5 Set gradient mode ......................................................................................... 47
B.2.6 Set pump type ................................................................................................. 47
B.2.7 Set compression compensation .............................................................. 47
B.2.8 Set mixer operation ....................................................................................... 48
B.2.9 Set valve operation ........................................................................................ 48
B.2.10 Setup language ............................................................................................... 48
B.2.11 Setup unit number ......................................................................................... 49
B.2.12 Setup display angle....................................................................................... 49
B.3 Alarm timer .......................................................................................... 49
B4 Service displays................................................................................. 50
B.5 Menu overview .................................................................................. 51
C Technical specifications
C.1 Operating data .................................................................................. 53
C2 Physical data ...................................................................................... 54
D Accessories and spare parts
6 Short instructions
12 59
About this manual

This manual comprises two parts; a practical part (sections 1 – 5) and a reference part
(sections A – D). Sections 1 – 5 contain the necessary information for operating the
instrument.

Contents

Introduction 1
1 Introduction
1.1 General
Pump P-900 is the collective name for a pump family. The family members
are:
• P-901 stand-alone or system mounted

Used as stand-alone, or in ÄKTA™explorer 100. Equipped with four pump
heads in two pump modules.
• P-903 stand-alone or system mounted

Used as stand-alone, or in ÄKTA™purifier 10/10 XT and ÄKTAexplorer
10/10 XT. Equipped with four pump heads in two pump modules.
P-9
00
Out to system
Fluid inlet tubing
P-901, P-903

1 Introduction
All P-900 models are high performance laboratory pumps for use in liquid
chromatography and other applications where accurately controlled liquid
flow is required. Double mode can be selected to double the flow rate range.
Pump P-900 features:
• P-901
– Pressure range 0–10 MPa (100 bar, 1450 psi).
– Flow rate range 0.01–100 ml/min isocratic and gradient mode.
– Double mode flow rate range of 0.01–200 ml/min isocratic mode.
• P-903
• The pumps are equipped with 2 pump modules, A and B, including two
pump heads each. This allows for binary gradients with high pressure
mixing.
• All pumps have a pressure sensor connected to pump module A.
• Low pulsation.
• Switch-valve control for low pressure gradients and BufferPrep.
All versions are run with the same software. Normal or double mode, as well
as the size of the pump heads are stated in a Setup meny, see Reference
information B.2.
1.1.1 Accessories
Accessories can be connected to the pumps, and can be controlled from the
pumps or from a PC running UNICORN™ version. 2.10 or higher.
These accessories are:
• Mixer M-925.
• 2-way Switching Valve SV-903.

Introduction 1
1.2 Safety
IMPORTANT! Pump P-900 is intended for laboratory use only, not for
clinical or in vitro use, or for diagnostic purposes.
• The instrument is designed for indoor use only.
• Do not use in a dusty atmosphere or close to spraying water.
• Do not block the air inlet and outlet of the unit.
WARNING! Always disconnect the power supply before attempting to

replace any item on the instrument.
WARNING! Incorrectly fitted tubing may loosen, causing a jet of liquid

to spray out. This is especially dangerous if hazardous chemicals are in
use. Connect the tubing by first inserting the tubing fully, then tightening
the connector fingertight. Finally tighten the connector a further 1/4 turn
using the key supplied.
WARNING! When using hazardous chemicals, all suitable protective

measures, such as protective glasses, must be taken.
WARNING! The instrument must not be opened by the user. It contains

high voltage circuits which can give a lethal electric shock.
WARNING! The instrument must be connected to a grounded mains

socket.
WARNING! NaOH is injurious to health. Avoid spillage.
WARNING! When using hazardous chemicals, take all suitable protective

measures, such as wearing protective glasses and gloves resistant to
the chemicals used. Follow local regulations and instructions for safe
operation and maintenance of the system.
WARNING! This is a Class A product. In domestic environment this

product may cause radio interference in which the user may be required
to take adequate measures.

1 Introduction

Installation 2
2 Installation
2.1 Unpacking
Unpack the instrument and check the items against the supplied packing
list. Inspect the items for obvious damage which may have occurred during
transportation.
It is recommended that all packing materials should be retained if onward
transport of the instrument is expected.
CAUTION! The following information should be read carefully to ensure

that the instrument is installed correctly.
2.2 General precautions

The instrument should be installed in a non-corrosive atmosphere.
The instrument should be located in a place of low temperature variations,
away from heat sources, draughts and direct sunlight.
The instrument may be operated at normal ambient temperatures in the
range +4 to +40 °C.
The instrument should be installed on a stable laboratory bench, providing a
suitable working area or in an ÄKTA system. To ensure correct ventilation a
free space of 0.1 m is required behind and in front of the instrument. Do not
block the ventilation inlets or aoutlets on the system! Place the instrument
P-900 directly on the bench. Do not use any soft material under the instrument, to
ensure that the ventilation inlet in the front is not blocked.
2.3 Connecting electrical signal cables

The sockets for electrical signals are located on the rear panel.
Valve 1
Valve A
Valve B
Pressure,
analog
out 0 - 1V UniNet 2 Remote UniNet 1 Mains
WARNING! Pump P-900 must be connected to a grounded mains socket

to prevent system parts from becoming live.
WARNING! Do not block the rear panel of the system. The mains power
switch must always be easy to access.

2 Installation
2.3.1 Connecting to chart recorder (if used)

1 Connect the chart recorder to the Mini-DIN-socket Pressure using the
cable supplied.
Pin Signal
1 Signal
2 Signal ground
3–6 Not used (Reserved for factory testing)
Note: The signal cable is delivered with protective covers on each wire.
Do not remove the protective covers from unused connections as a
short circuit may disturb the measurements.
2 Set the recorder to 0–1 V input, full scale.
3 Refer to section B.2.2 for a description of setting the full scale pressure.
2.4 Connecting to communication link

The pumps used in ÄKTAexplorer and ÄKTApurifier, are controlled from a PC
running UNICORN version 2.10 or higher, using UniNet cables.
CAUTION! The mains power to ÄKTAexplorer and ÄKTApurifier must be

switched OFF before connecting the instrument to the UniNet 1 link.
1 Connect two UniNet cables to the UniNet 1 connectors. The instrument

can be connected in series anywhere in the chain between the PC and
the termination plug. The UniNet 1 link connects, in series, the PC with
Pump P-900 and other instruments. The termination plug is connected to
the last instrument in the chain.
2.5 Connecting to supply voltage

1 Make sure the on/off switch is in the OFF-position O.
2 Connect a mains cable between the instrument and a grounded mains
socket. The instrument is delivered with both European and US type
mains cables, as standard. Any voltage from 100–240 V AC, 50–60 Hz
can be used.
WARNING! The instrument must be connected to a grounded mains

socket.
The instrument contains no user replaceable fuse.
WARNING! Only use mains cables delivered or approved by GE

Healthcare.

Installation 2
2.6 Installing the piston seal rinsing system

Note: For the protection of the new pump, do not run the pump until the
system has been flushed out and primed with eluent, according to
sections 2.8 and 2.9.
The rinsing system for the piston seals must be installed before use.
20% ethanol should be passed around the piston and piston seal on the
non–pressure side of the piston seal. This protects the piston seals and
pump heads by preventing a build-up of deposits from the solvents.
1 Connect the tubing for the piston seal rinsing system. The connections
are simple plug-in fittings.
2 The three lengths of wide bore tubing connect the pump heads in series,
connecting the top of one pump head to the underside of the next. The
wide bore tubing with the check valve should be fitted between the right
pump head of pump module A and the left pump head of pump module
B. The arrow on the check valve must point towards pump A.
A B 3 Two lengths of narrow bore tubing are also supplied with the pump.
Connect the tubing with a Union Luer connector to the underside of
P-901, P-903
the left pump head on pump A. Connect the other to the top of the
right pump head of pump module B and immerse the tubing in a flask
containing rinsing solution (20% ethanol).
4 Connect a syringe to the rinsing tubing that is connected to the

underside of the left pump head on pump A. Slowly suck up rinsing
solution to the syringe. When rinsing solution starts to enter the syringe,
continue to draw a few millilitres.
5 Loosen the syringe and immerse the tubing in the rinsing solution.

2 Installation
2.7 Connecting the inlet tubing

The inlet tubing is connected to the centre port of the inlet manifold of each
pump module.
Connect the tubing using male 5/16”-connections.
2.8 Purging the pump

CAUTION! To protect the piston seals, the pump must never be run with
air in the inlet tubing. Follow the procedure below to remove the air.
Purge valve
Purge tubing
1 Fill a reservoir flask with distilled water. Immerse the inlet tubings of both
pump modules, with filters, in the water.
Note: Never place the reservoir flask below the level of the pump inlet.
2 Connect a male Luer syringe of about 30 ml to the open end of the

purge tubing.
3 Connect the male Luer connector at the other end of the purge tubing to
the left purge valve at pump module module A.
4 Turn the purge valve counterclockwise half a turn to open it and slowly
draw eluent to the syringe.
5 When fluid starts to enter the syringe continue to draw a few millilitres
before closing the purge valve. Check that there is no air left in the inlet
tubing.
6 Repeat steps 3 to 5 for pump module B, if fitted.

Installation 2
2.9 Running-in the new pump

2.9.1 General
To be able to follow this section you must know how to operate the pump.
Read through section 3 Operation before continuing.
Note: To ensure trouble-free operation of the pump, it is important hat this

running-in procedure is followed.
The pump heads are filled with 20% ethanol for protection during
transportation. This is removed by running-in the pump with a liquid such
as distilled water which is miscible with 20% ethanol. After running-in, the
pump is primed for the chromatographic run.
Note: If, for any reason, the pumpheads are dry, actions according
to section 4.5 Running in a new piston seal must be carried out
instead!
2.9.2 Running-in
1 Purge the pump, see section 2.8 Purging the pump.
2 Switch the mains power switch on the rear panel to ON.
3 Check that the outlet tubing are not blocked, and press OK in the pump
synchronization display.
4 Set the flow rate in menu Set Flow Rate. Run both pumps at the same
time by setting the concentration to 50% in menu SetConcentrationB
under menu SetConc./Gradient. Set the pump in Run mode. Run the
pump at the flow rates and times specified below.
Pump Flow rate Time

P-901 40 ml/min 10 minutes
P-903 4 ml/min 10 minutes
5 Stop the flow by setting the pump in End mode.

2 Installation
2.10 Connecting the pump outlet tubing

1 Remove the blind plug from the pump head outlet manifold. Connect the
outlet tubing using 1/16” Fingertight connectors. Place the end of the
tubing in a drain or waste collection vessel.
2 Close the purge valve by turning it clockwise.

Purge valve
Outlet tubings
3 Set the flow rate to 10 ml/min for P-901, and to 1 ml/min for P-903.
4 Set the pump in Run mode. Allow the pump to run for 1–2 minutes to
prime the new outlet tubing.
5 Stop the flow by setting the pump in End mode.
2.11 Installation of accessories

Mixer M-925 and up to three 2-way Switching Valves SV-903 can be
connected to the pump:
0, 6
M-9
25
Mixer M-925 Switch Valve SV-903

Installation 2
CAUTION! Before connecting any accessories, make sure the power

switch is in OFF position.
Mixer M-925 is connected to the UniNet-2 connector.
SV-903 valve for buffer selection is connected to Valve 1. The valve is set
on/off via the pump sub menu Set Valve 1 under the Setup menu. See
Reference information B.2.8.
SV-903 valves for BufferPrep are connected to Valve A and Valve B.
SV-903 for one-pump gradients is connected to Valve A.
Valve 1
Valve A
Valve B
Pressure,
analog
Out to system
0, 6
M-9
25
Fluid inlet tubing

2 Installation

Operation 3
3 Operation
3.1 On/off
Switch on the instrument at the mains switch on the rear panel. At switch on
the instrument performs a selftest and then asks for synchronization.
Open the purge valves (see section 2.8) to avoid high pressure during
synchronisation which is done at the speed of 0.1 ml/min, and press OK.
The display will show Synchronizing pumps and after approx. 1 minute the
display shows the main operating menu with the pump in End mode. Close
the purge valves. All parameters are factory set to default values.
3.2 Menu selection and settings

3.2.1 Menu selection
A specific menu is selected by turning the front selection dial clockwise or
counterclockwise. When the required menu is visible the menu or selection is
accepted by pressing the OK-button.
Menu selection OK-button ESC-button

Select submenu Return one menu level
If a menu has sub levels, the sub menu is displayed by pressing the OK-
button. Pressing the ESC-button moves back one menu level.
Main menus Sub menus Sub menus
Main menu 1
Main menu 2 OK OK
Sub menu 2.1 Sub menu 2 .1.1
ESC ESC
Main menu 3
Sub menu 2.2
Main menu 4 OK
Sub menu 2.3 Sub menu 2.3.1
ESC
Main menu 5
Sub menu 2.3.2

3 Operation
3.2.2 Return to main menu

Pressing ESC repeatedly, always returns to the main menu 2 which is the
main operating menu. Press ESC once more to return to main menu 1, the
mode changing menu.
Main menu 1
ESC
Main menu 2 ESC ESC ESC ESC
3.2.3 Select value

A cursor below a text or numerical value shows what is affected by the dial.
To increase the value turn the dial clockwise. To decrease the value turn the
dial counterclockwise. The value can be reset by turning the dial several
clicks counterclockwise.
Parameter Set Hi Press Limit

(10.00MPa) 50.0
Current value New value to be set
When setting numerical values the cursor moves up to the next digit if the
dial is turned quickly in one direction, to simplify entering large values. The
cursor moves back one place to the right every two seconds if the dial is not
turned. The text or numerical value displayed is accepted by pressing the K-
button. To cancel, press the ESC-button.
3.3 Main menu overview

Mode changing menu. This is from where the pump is started and stopped.
The menu is accessed from all positions by pressing the ESC-button
repeatedly. The appearance of this menu will depent on the current mode.
Main operating menu: The menu is accessed from all positions by pressing
the ESC-button repeatedly.
Setting flow rate in ml/min.
Setting concentration and gradient values.
Check internal operating values. See Reference information section B.1.
Setup language, pressure limits, etc. See Reference information section B.2.
Set different timer options. The pump can be started or stopped at set times.
See Reference information section B.3.

Operation 3
3.4 Starting and stopping the pump

3.4.1 Main operating menu

The main operating menu shows the current flow rate together with a mode
indication, pressure and %B, if used. The available modes are:
Run The pump is running with set flow rate

End The pump is not running. Flow rate and gradient are reset.
Pause The pump is stopped but the set flow rate value and the gradient
are retained.
Hold The gradient is held at the value displayed, and the pump
continues to run.
3.4.2 Mode changing menu

Mode changes are made in the mode changing menu above the main
operating menu (turn dial counter-clockwise). The current mode is shown
in the upper left corner of the display. Available actions are shown at the
lower right. There are four different displays for this menu and the menu
displayed will depend on the current mode. When a new mode is selected
the appearance of the menu will change.
Pressing OK in a mode changing display will select the underlined mode.

Different modes can be underlined by turning the dial.
To start the pump: Select Run and press OK.

To stop the pump: Select End and press OK.
3.4.3 Preparation before starting
CAUTION! Before the start of each run, always ensure that there is an
adequate supply of eluent in the reservoirs. Never allow the pump to run
dry, since this will affect the lifetime of the piston seals.
1 Check that there is sufficient eluent present for the run, and that the
solvent filter is fully immersed. If the eluent is to be changed,
see section 3.9 Changing eluent
Note: The pump may not work if the buffer vessels are sealed. Do not
close the vessels off completely.
3 Operation
2 If there is air in the tubing or the pump, prime the pump. Refer to
section 2.8 Purging the pump.
3 Check that there is sufficient liquid (20% ethanol) in the rinsing system
reservoir.
4 Set the pressure limit. Refer to section B.2.1 in Reference information.

If the pressure limit is exceeded, the pump is stopped.
3.4.4 Emergency stop

By pressing ESC repeatedly and ending with OK, the pump is set to Pause
mode.
3.5 Setting the flow rate and starting the pump

1 Select main menu Set Flow Rate, press OK.
2 Set the value, press OK.
3 Select the mode menu, select Run, press OK. The pump speed gradually
increases to deliver the set flow rate.
4 To change the flow rate while the pump is running, perform steps 1 and
2 again.
5 To stop the pump, select the mode menu, select End, press OK.
In UNICORN select instruction Flow in System Control:Manual:Pump. Click

on Execute and the pump will start.
3.6 Setting concentration B

A percentage of eluent B can be set.
1 Select main menu Set Conc./Gradient, press OK.
2 The display shows Set Concentration B, press OK.
3 Set the %B value, press OK.
In UNICORN, select instruction Gradient in System Control:Manual:Pump.

Set length to zero.

Operation 3
3.7 Running a simple gradient

Gradients can be run in time or volume base. The default is time base. To
change base, see B.2.4 in section Reference information.
The gradient is run from current concentration to target concentration, in

set length of time or volume. The gradient can be set in any operating mode.
100%
Gradient length Target
concentration
Current
concentration
0%
Gradient
started
1 Select main menu Set Conc./Gradient, press OK.
2 The display shows Set Concentration B. Turn the dial to select menu Set
Gradient Length, press OK.
3 Set the value, press OK.
4 Select menu Set GradientTarget. Set the gradient target value in %B,
press OK. If the pump is in Run mode the gradient will start immediately.
5 At the end of the set gradient length time, the pump continues to run at
the target concentration.
In UNICORN, select instruction Gradient in System Control:Manual:Pump.
3.8 Ending the run and storage

The pump can be left filled with a buffer overnight.
Note: If buffers or water are stored at room temperature, there is a risk

that bacterial growth may occur.
If no further runs are planned, the pump should be flushed immediately with
pure eluent. If aqueous buffers have been in use, flushing with pure distilled
water is particularly important to prevent salt precipitation.
Overnight storage: The pump can be left filled with a buffer.
Weekend and Long time storage: Flush the pump with water and then fill it
with 20% ethanol.

3 Operation
3.9 Changing eluent
CAUTION! To prevent precipitation of crystals when changing from a salt-

containing buffer to organic solvent, always flush through the system
with water as the intermediate liquid.
When changing from one eluent to another, it is extremely important that

the two eluents are totally miscible with one another. If the two eluents are
immiscible, the pump should be flushed first with an intermediate liquid,
which is miscible with both eluents. Failure to do this will cause a wrong flow
of eluent from the pump.
When changing from a salt-containing buffer to an organic solvent, use

water as the intermediate liquid to prevent precipitation.
1 Stop the pump by setting it in Pause mode.
2 Transfer the inlet tubing into the new eluent or into the intermediate
liquid.
3 Run the pump at a flow rate and time as specified in the table in
section 2.9.
4 Stop the pump. If an intermediate liquid is being used, transfer the inlet
tubing into the final eluent and repeat step 3 with the new eluent.
In UNICORN, select instruction PumpWash in System Control:Manual:Pump.
3.10 Restart after power failure

If the power supply to the instrument is interrupted, the instrument
automatically restarts itself doing selftest and prompting for
synchronisation. All values under the Setup menu are retained. Other
values, e.g. flow rate are reset.

Maintenance 4
4 Maintenance
Note: The piston seals have a limited life time depending on the flow rate,
pressure and eluents used. The seals are regarded as consumables
and are available as a service kit. The wear of the piston seals is
not covered by the warranty of the instrument. A typical lifetime is
around 1000 run hours
WARNING! Remove liquid or dirt from the system surface using a cloth
and, if necessary, a mild cleaning agent.
WARNING! Always disconnect the power supply before attempting to

replace any item on the instrument during maintenance.
CAUTION! Only spare parts approved or supplied by GE Healthcare may

be used for maintaining and servicing the instrument.
4.1 Periodic maintenance

Interval Action (see procedures below)
Daily General care
When required Removing trapped air bubbles from the pump
Fixing leaking connections
Replacing the piston seal
Replacing a damaged piston
Cleaning or replacing the inlet and outlet check valves
4.2 Cleaning-in-place
Pump a cleaning or sanitizing agent through the pump. The standard
recommendation is to pump 1 M NaOH for 30 minutes and then wash out
with buffer or distilled water.
WARNING! NaOH is injurious to health. Avoid spillage.
WARNING! When using hazardous chemicals, take all suitable protective

measures, such as wearing protective glasses and gloves resistant to
the chemicals used. Follow local regulations and instructions for safe
operation nd maintenance of the system.
WARNING! When using hazardous chemicals, make sure that the entire
system has been flushed thoroughly with bacteriostatic solution, e.g.
NaOH, and distilled water before service and maintenance.

4 Maintenance
4.3 General care

Inspect the complete system daily for eluent leaks.
• Wipe the surface regularly with a damp cloth. Do not allow spilt liquid to
dry on the instrument.
• Remove dirt from the surface using a cloth and a mild cleaning agent.
• Let the system dry completely before using it.
If, at any time, air is observed in the inlet tubing, purge the pump by opening
the appropriate purge valve and running the pump at max. flow rate for
about 1 minute. If there is a lot of air in the inlet tubing, stop the pump and
perform the procedure in section 2.8 Purging the pump.
4.3.1 General recommendations for all eluents

It is essential that all liquids passing through the pump are clean and pure.
Impure or dirty eluents will not only cause baseline noise and drift, but will
cause damage to the pump head assemblies.
4.3.2 Additional recommendations for aqueous eluents

After running with an aqueous eluent the pump should always be
thoroughly purged with pure, distilled water to prevent salt precipitation.
4.4 Replacing the piston seal

If there are signs of liquid leaking between the pump head and the housing
side panel or the volume of the rinsing solution has increased or decreased,
replace the piston seal of the leaking pump head.
CAUTION! Do not disassemble the pump head unless there is good

reason to believe that the seal is leaking. Always ensure that sufficient
spare components are available before attempting to replace the piston
seal. It is not possible to reinstall a used piston seal after removal.
Note: The power must be switched OFF when removing and refitting the
pump heads.
Note: Always replace the piston seals on both pump heads at the same
time. An even better practice is to replace all four piston seals.
Spare parts and tools required:

Seal kit containing (see Reference information Dfor code no.):
2 or 4 piston seals
2 or 4 rinse membranes
1/4 inch wrench (supplied with the pump)
3 mm allen key (supplied with the pump)
Screwdriver (supplied with the pump)

Maintenance 4
Note: After a new seal has been installed, the pump should be run in, see
section 4.5 Running-in a new piston seal.
Note: Before disassembling the pump heads move all input buffers bottles
below the level of the pump heads to prevent siphoning.
CAUTION! Read the following instructions carefully. The individual parts

of the pump head can be assembled incorrectly. Take care to ensure that
the orientation of each part is correct before continuing with the next
instruction.
1 Switch off the pump at the mains power switch on the back panel.
Remove the piston seal rinsing system. The connectors are simple plug-
in fittings.
2 Completely loosen the tubing connector on the outlet valve.
3 If the pump is equipped with Switch Valves SV-903, first remove the switch
valves and disconnect the tubing. Unscrew the two white knurled screws
Outlet valve under the pump to release the support bracket locking the inlet manifold
into the inlet valve. Gently lower and remove the complete manifold.
4 Using the Allen key, unscrew and completely remove one of the two
Allen screws locking the pump head in position.
5 When unscrewing the second locking screw, push firmly on the front
face of the pump head to compensate for the pressure of the piston
return spring. Hold the pump head firmly to prevent it from twisting.
Remove the second screw and, without allowing the pump head to twist
sideways, carefully pull it out.
6 Place the pump head face down on the bench. Pull out the piston
together with the return spring.

4 Maintenance
7 Inspect the piston and return spring for sign of damage. If damaged,
they should be replaced.
8 Wipe the piston with a clean cloth. If salt solutions have been used
the piston may be slightly corroded. This corrosion can be removed
with a rubber eraser. If it cannot be wiped or rubbed clean, scrape
off any deposits with a scalpel or razor blade. Inspect the piston with
a magnifying glass for scratches. Replace with a new piston if any
scratches or cracks are found.
9 Remove the two screws securing the drain plate and the rinse chamber.
Remove and discard the rinsing membrane. Remove the rinse chamber.
For P-903, remove also the support washer.
Rinsing membrane
Drain plate
Rinse chamber
10 Gently withdraw the piston seal. Discard the used seal.
11 The pump head, rinse chamber and drain plate should be carefully
rinsed or cleaned in an ultrasonic bath, if available. If dirt can be seen on
any surfaces, the inlet and outlet check valves should be removed and
cleaned separately (see section 4.7)
12 Wet the new seal slightly and place it in the hole on the pump head and
press it down into position with a hard flat object For P-903, refit the
support washer on top of the new seal.
13 With the pump head still facing downwards on the bench, place the
rinse chamber onto the head with the rinse ports in line with the inlet
and outlet check-valves. The conical depression in the rinse chamber
should be facing upwards, ready to accept the new rinsing membrane.
Fit the rinsing membrane with the conical face downwards.

Maintenance 4
Return spring Piston Return spring

Piston
Drainage hole Drainage hole
Drain plate Drain plate
UP UP
Rinse membrane Rinse membrane
Rinse chamber Rinse chamber

outlet outlet
Support washer
Inlet Inlet
check valve Jointing ring check valve
Piston seal
Piston seal
Outlet Outlet
check valve check valve
P-901 P-903
14 Place the drain plate on top of the assembly. Use the two screws to lock
the complete assembly together.
Note: Align the drainage hole in the drainage plate with the inlet check
valve (the opposite side of the pump head marked OUT/UP).
Inlet check
valve
15 Wipe clean the piston and remove all finger prints. Wet the piston
UP
and then insert it into the return spring. With the pump head facing
downwards on the bench, insert the piston into the pump head by
pushing it gently but firmly vertically downwards into the seal.
CAUTION! Do not push the piston at an angle to the head and DO NOT
twist the piston.

4 Maintenance
16 Turn the head so that the inlet valve and drainage hole are facing
downwards and the text UP/OUT on the pump head is facing upwards.
Mount the complete pump head over the locating pins on the front
panel. Locate the metal end of the piston and the spring towards the
drive cam.
Hold the pump head firmly against the side panel of the housing with
one hand. Do not allow the assembly to twist under pressure from the
return spring. Using the Allen key, fit and tighten one of the Allen screws.
Fit and tighten the remaining Allen screw.

17 Reconnect the outlet tubing to the outlet check valve and the manifold
block as described above.
18 Reconnect the inlet manifold and, if used, the switch valves.
19 Refit the tubing of the piston seal rinse system.
20 The pump should now be purged and the new piston seal carefully run-
in following the instructions in section 4.5.

Maintainence 4
4.5 Running-in a new piston seal

The piston seal should be run-in using 100% methanol.
CAUTION! To protect the pump seals, always ensure that there is a

constant supply of eluent. The pump should never be allowed to run dry.
1 Ensure that the reservoir is filled with sufficient eluent. Immerse the
inlet tubing in the eluent. The reservoir should be placed at least 30 cm
above the pump inlet.
2 Connect a male Luer syringe of about 30 ml to the open end of the

purge tubing.
3 Connect the male Luer connector at the other end of the purge tubing
to the left purge valve at pump module module A.
4 Turn the purge valve counterclockwise half a turn to open it and slowly
draw eluent to the syringe.
5 When fluid starts to enter the syringe continue to draw a few millilitres
before closing the purge valve. Check that there is no air left in the inlet
tubing.
6 Repeat steps 3 to 5 for pump module B, if fitted.
7 Check that the outlet tubings are not blocked.
8 Connect a thin capillary or a column that will give sufficient back

pressure.
9 Run at the following flow rates for 15 minutes:

P-901 1 ml/min (or 2 ml/min 50%B)
P-903 0.1 ml/min (or 0.2 ml/min 50%B)
10a For P-901, run at 20 ml/min (or 40 ml/min 50%B) at a backpressure of

2–5 MPa for 15 minutes.
10b For P-903, run at 2 ml/min (or 4 ml/min 50%B) at a backpressure of

5–10 MPa for 2 hours, or longer if possible (e.g. overnight).
11 Finally, change the eluent according to the description given in

section 3.9.

4 Maintenance
4.6 Replacing a damaged piston

Typical symptoms of a damaged piston are observed as excessive piston
seal wear, unstable pressure, a reduction in the flow or, in some cases, noise
as the piston moves. The piston should be removed, examined for damage
or salt precipitation and then replaced with a new piston if necessary.
If a damaged piston has been in operation, the piston seal will be destroyed
and should also be replaced. To replace the piston and the seal follow the
instructions in section 4.4 Replacing the piston seal.
In addition to the spare parts listed in section 4.4, the following are required
(see Reference information D for code no.):
P-901, Piston kit, 100 ml

or:
P-903, Piston kit, 10 ml
4.7 Cleaning the inlet and outlet check valves

Faulty operation of the check valves is usually indicated by irregular flow,
very low flow or unstable pressure traces. Probable causes of this are air or
dirt in a check valve preventing it from closing to seal and hold the pressure.
Record the pressure (see section 5.4) and identify the faulty check valve by
observing which pump head is delivering the flow (see section B.1.1).
First try to clean the check valves in–place on the pump head by pumping
100% methanol for approximately 10 minutes. If this does not correct the
problem, follow the instructions for removing and then cleaning the valves.
Note: Change solvent to distilled water and flush out all salt before
removingthe check valves.
Tools required: A 13 mm and a 1/4 inch wrench.
4.7.1 Cleaning after removal

Before removing the check valves, move all input buffers bottles below the
level of the pump heads, to prevent siphoning.
1 If the condition of the check valve is not improved by in–place cleaning,

disconnect and remove the inlet manifold and outlet tubing.
2 Use the 13 mm wrench to remove the valve from the pump head.
CAUTION! Handle the check valves with care when they have been
removed from the pump heads to prevent loss of any internal
components.

Maintenance 4
3 Immerse the complete valve in methanol and place in an ultrasonic bath

for some minutes.
Then repeat the ultrasonic bath with distilled water.
4 Refit the check valves. The inlet check valve (with a lip for the manifold
and a larger diameter opening) is fitted to the side marked IN of the
pump head. Tighten the valves until fully finger-tight and then use the
13 mm wrench to tighten a further 1/3rd (110o) of a turn. Do not over-
tighten the valves since damage to the internal components can occur.

5 Refit the outlet tubing and the inlet manifold.
6 Purge the pump carefully and check that the pumping action has been
corrected. See section 2.8.
Note: Check valves have precision matched components and should

only be disassembled further by a trained service engineer. If
the problem cannot be corrected, the valve should be replaced
completely.

4 Maintenance

Trouble shooting 5
5 Trouble shooting
5.1 General
When contacting GE Healthcare for support, state the program version of
the instrument, shown for 2 seconds during switch-on.
It is recommended to make a pressure recording since much information

can be gained from the pressure trace. see section 5.4 Checking the pump
pressure, for more information.
WARNING! The instrument must not be opened by the user. It contains

high voltage circuits which can give a lethal electric shock.
5.2 Faults and actions

If the suggested actions do not correct the fault, call GE Healthcare.
Fault Action
No text on the front display 1 Check that the mains cable is onnected and
the power switch is in ON-position I.
2 Check the mains power supply.
Erratic flow, noisy baseline
signal, irregular pressure trace
Air bubbles passing 1 Check that there is sufficient eluent present in
the through or trapped in the pump reservoirs.
2 Check all connections for leaks.
3 Follow the instructions in section 5.5 Removing
trapped air bubbles from the pump.
Inlet or outlet check valves 1 Follow the instructions in section 4.7 Cleaning
not functioning correctly the inlet and outlet check valves.
2 Clean the valves in-place. If improvement is not
seen, remove the valves and clean again.
Piston seal leaking 1 Replace the piston seal according to the
instructions in section 4.4 Replacing the piston
seal.
Blockage or part blockage 1 Flush through to clear blockage.
2 If necessary, replace tubing.
3 Check inlet tubing filter. It can become clogged
if unfiltered buffers or samples are applied.
See instructions for flushing through at the end
of the run in section 3.8 Ending the run and
storage.
Liquid leaking between the Piston seal or rinsing membrane incorrectly fitted
or worn pump head and the 1 Replace or re-install the seal or membrane.
side panel 2 Run-in carefully, see section 4.5 Running-in a
new piston seal.

5 Trouble shooting
Fault Action
Low eluent flow and noise 1 Disassemble pump head and examine the
as the pistons move piston spring according to section
4.4 Replacing the piston seal. Replace if
necessary.
2 If spring is corroded, check piston seal and
rinse membrane. Ensure that piston rinsing
system is always used when working with
aqueous buffers with high salt concentration.
3 Check the piston for damage. If damaged,
replace the piston according to section
4.6 Replacing a damaged piston.
4 Remember to replace the piston seal and rinse
membrane with new items.
Leaking connection and/or 1 Unscrew the connector and check if it is worn
crystalized material around or incorrectly fitted. If so replace the connector.
a connector 2 Gently tighten the connector with your fingers.
Error in external chart recorder 1 Check the chart recorder in accordance with its
manual.
5.3 Error messages

If the suggested actions do not correct the fault, call GE Healthcare
Message Action
ERROR No mixer 1 Switch off the instrument.
Check connection! 2 Check the connections to the mixer.
ERROR in mixer. 3 Switch on the instrument.
Check mixer!
ERROR in valve x 1 Switch off the instrument.
Check connections! 2 Check the connections to the valve(s).
3 Switch on the instrument.
ERROR Pump too warm 1 Switch off the instrument to let it cool.
Check fans! 2 Clean or clear the front air inlet.
3 Switch on the instrument and check that the
fans are running.
ERROR Sync failure 1 Switch off the instrument.
Please restart! 2 Switch on the instrument. Remember to
open the purge valves before synchronization.
ERROR key (OK) 1 Switch off the instrument.
ERROR key (Esc) 2 Check all connections.
ERROR key (OK+Esc) 3 Switch on the instrument.
ERROR 100
ERROR 109-113
ERROR 120-121
ERROR 106-108 1 Switch off the instrument.
ERROR 118 2 Check all UniNet1 and UniNet 2 connections.
3 Switch on the instrument.

Trouble shooting 5
5.4 Checking the pump pressure

To check the pump function, a recording of the pressure can be made
(see section B.2.2), or by checking the pressure in UNICORN. This pressure
recording is more sensitive than the reading on the display. By observing
the piston stroke indicator in the Check menu (see section B.1.1) together
with the pressure trace, the pump head which is functioning abnormally can
be identified.
There can be several causes of an abnormal pressure recording, for

example:
• air trapped in the pump heads
• partially blocked solvent filters
• leaking connections
• piston seal leakage
• check valve malfunction
• piston damaged
Some examples of normal and abnormal pressure traces together with

comments are shown in the following table.
Result Running conditions Diagnosis

P-901 Flow rate: 1.00 ml/min Pump performance
Left Right Left Pressure: 2.0 M Pasatisfactory
Recorder 1 V, 1 mm/s Active head, left and right are shown
0.5 MPa Pump setup: Full Scale The small pressure peaks are caused by the
5s 10 MPa pump principle
Flow rate: 30 ml/min Pump performance satisfactory

Pressure: 7.5 MPa
0.5 MPa Recorder 1 V, 1 mm/s
Pump setup: Full Scale
5s
10 MPa
P-903 Flow rate: 1.00 ml/min Pump performance satisfactory

Pressure: 12.0 MPa
Recorder 1 V, 2 mm/min
1 MPa Pump setup: Full Scale
5 min 25 MPa

5 Trouble shooting
Result Running conditions Diagnosis

P-901 Flow rate: 1.00 ml/min Left pump head OK, right
Pressure: 2.0 MPa pump head not working
Left Right Left
Recorder 1 V, 1 mm/s Possible causes:
Pump setup: Full Scale • Bad or missing seal
0.5 MPa
10 MPa • Large leakage in inlet check valve
5s • Inlet opened or blocked
Flow rate: 20 ml/min Air bubbles passing through pump heads,

Pressure: 4.3 MPa then the trace returns to normal
0.5 MPa Recorder 1 V, 1 mm/s
Pump setup: Full Scale
5s
10 MPa
P-903 Flow rate: 2 ml/min One pump head stops

Pressure: 4.3 MPa working. Possible causes:
Recorder 1 V, 1 mm/s • Air bubble stuck in pump
Pump setup: Full Scale head
0.5 MPa 10 MPa • Large leakage in one pump head
5s • Large leakage in one inlet check valve
• Inlet open or blocked
5.5 Removing trapped air bubbles from the pump

During routine operation, the presence of air bubbles in the pump heads
is seen as an erratic flow, a noisy detector signal or an irregular pressure
recording. With air bubbles trapped in the pump, the pressure reading on
the display will fluctuate considerably.
To remove the air from the pump:
1 If the air has accumulated because of a leaking connector, correct the

fault.
2 Ensure that the inlet filter is fully immersed in eluent.
3 Purge the pump according to section 2.8 Purging the pump.
If the problem remains, try to remove the air bubbles in accordance with the
method described below. Use 100% methanol.
1 Run at 40 ml/min for P-901, or 4 ml/min for P-903.
2 Continue to run for 10 minutes, or until the air bubble is removed.
3 Change the eluent according to the description given in section 3.9.

Reference information A
A Description
A.1 Instrument
Pump P-900 is a high performance laboratory pump for use in liquid
chromatography and other applications where accurately controlled liquid
flow is required.
An eluent in an external vessel is drawn into the inlet manifold by the action
of the pump. Twin reciprocating pump heads work in unison to deliver a
smooth, low-pulsation flow from the pump outlet manifold.
Pump P-900 features:
• P-901
• P-903
• The pumps are equipped with 2 pump modules, A and B, including two
pump heads each. This allows for binary gradients with high pressure
mixing.
• All pumps has a pressure sensor connected to pump module A.

P-9
00
• Low pulsation.
• Switch-valve control for low pressure gradients and BufferPrep.

A Reference information
Valve 1
Valve A
Valve B
Pressure,
analog
Connector/switch Function
Pressure analogue out 0-1V Pressure signal to chart recorder
UniNet 2 Connection to mixer M-925 and UniNet 2 network
Remote Input for remote on/off and output for pressure alarm
UniNet 1 Connection UniNet-1 network
Mains Supply voltage, grounded
0/1 Instrument on/off switch
Valve A, B Connection to switch valves. A and B used for
BufferPrep. When pump used for one pump
gradients as stand-alone, valve A should be used.
Valve 1 Connection for an additional switch valve for buffer
selection when used as stand–alone
The instrument contains no internal user replaceable items.
Connect any auxiliary equipment to the 9-pole D-SUB female REMOTE

connector (5 V TTL signals only).
Pin Signal Function

Active status = low or closed terminal to pin 5 (0 V)
1 Remote
run/pause active = pause
2–4 –
5 0V Signal ground
6 Pressure
alarm output active = alarm
7–9 –
When used in combination with UNICORN and a custom-designed strategy,

4 digital inputs and 4 digital outputs can be handled via the REMOTE
connector. Pin 1–4 can then be used as inputs, and pin 6–9 as outputs. All
input/output signals are 5 V TTL signals.

A.2 Pump head

The pump consists of one or two pump modules, A and B. Each pump
module consists of two pump heads. The individual heads are identical but
are actuated in opposite phase to each other by individual stepper motors
controlled by a microprocessor. This gives a continuous, low pulsation, liquid
delivery.
Each outlet check valve houses a purge valve, a fingertight connector. It is

used for draining any unwanted eluents or to remove air from the system.
The purge valve is opened by turning it counterclockwise half a turn.
The inlet to each pump head is fed from a common manifold. Solvent is
drawn up into the pump head through a non-return check valve by the
action of the piston being withdrawn from the pump chamber.
Outlet check
valves Outlet manifold Outlet, to mixer
Outlet check valve
Rinse chamber
inlet
U
Inlet manifold
Inlet Inlet check
valve

On the delivery stroke of the piston, the inlet valve is sealed by the pressure
developed and eluent is forced out through a similar check valve at the
outlet.
Return spring Piston Return spring

Piston
Drainage hole Drainage hole
Drain plate Drain plate
UP UP
Rinse membrane Rinse membrane

outlet outlet
Support washer
Inlet Inlet
check valve Jointing ring check valve
Piston seal
Piston seal
Outlet Outlet
check valve check valve
P-901 P-903
The pistons are actuated by cams (eccentrics) driven by the motors. Force
for the retraction of the pistons is provided by coil springs. The length of
stroke of the pistons is fixed and changes in the flow rate are made by
varying the speed of the drive motor.
Leakage between the pump chamber and the drive mechanism is prevented
by a piston seal. The pistons and seals are continuously lubricated by the
presence of eluent. To prevent any deposition of salts from aqueous eluents
on the piston and to prolong the life of the seals, the low pressure chamber
behind the pump head can be flushed continuously with a low flow of
distilled water. The flexible rinsing membrane sweeps the piston and seals
the rinsing system.
The pump head is manufactured in titanium alloy.

A.3 Pump principle

Each piston is driven by a simple robust cam (eccentric). These cams
are driven by stepper motors via timing belts. The motor speed is varied
to achieve linear movement and compensation for compressibility. This
produces the particular motor sound. This system guarantees an accurate,
low pulsation flow over the entire flow rate range, independent of the back
pressure. When an increase in flow rate is programmed, the motor speed
accelerates gradually, giving a soft start and building up speed to the flow
rate required. When a decrease in flow rate is programmed, the motor
speed reduces rapidly to the lower flow rate.
A.4 Outlet manifold

The outlet of each pump is connected by tubing to a manifold block where
the liquids from both heads are combined to give continuous eluent delivery.
The tubing and the manifold block are made in PEEK. The manifold block
houses the outlet connections to the mixer.
Outlet tubing
Purge tubing
Purge valve

A.5 Pressure monitoring

The pressure in the system is continuously monitored by the pressure
transducer housed in the outlet manifold block of pump module A. In a two-
pump module configuration with the pump modules connected to a mixer,
the pressure is (almost) the same in pump A and B.
The output pressure is shown in the display together with the flow rate.
A continuous recording of the pressure may be made using the analog
output. For the protection of the system, a maximum and minimum
pressure limit can be set.
A.6 Using an external chart recorder

The facility for recording the actual pressure at the pump outlet can be used
for recording the pressure during an overnight run and for checking the
pump function for any abnormality.
The external chart recorder output for pressure is 0–1 V. The full scale
range is set under sub menu Set Pr Full Scale under Setup, see Reference
information B.2.2.

Reference information B
B Menus
B.1 Check menu
B.1.1 Checking piston stroke
To enable trouble shooting it is possible to check which pump module head
that delivers flow.
1 Select main menu Check, press OK.

Check Heads 2.30MPa
Check Heads 2.30MPa 2 The display shows the status of the pistons for both pump modules. A:
Left means that the left pump head is delivering flow in the A pump and
B:Right the right pump head in the B pump. At the changing point both
are displayed. The A pump is closest to the front panel.
B.1.2 Check pump operation time

Check Total Run Time
Check Total Run Time 2 Select sub menu Check Total Run Time. The display shows the
accumulated operation time (Run and Hold mode) for the A and B pumps.
B.1.3 Check number of piston strokes

Check Piston Strokes
Check Piston Strokes 2 Select sub menu Check Piston Strokes. The display shows the
accumulated number of piston strokes for the A and B pumps.
Check Service Mode B.1.4 Check Service Mode

Check Service Mode
Service information relevant to the instrument can be checked. Information
Telephone Service may not be available in all menus.
012345678901
Telephone Service
012345678901
Contract Number 1 Select main menu Check, press OK.
012345678901
Contract Number
012345678901 2 Select sub menu Check Service Mode, press OK.
Serial Number
01234567 YM 012345
Serial Number 3 The service telephone number1 is displayed, press OK.
01234567 YM 012345
Pump P-900
V1.00
Pump P-900 4 The service contract number1 is displayed, press OK.
V1.00
Date of Maintenance 5 The instrument serial number is displayed, press OK.
?
Date of Maintenance
?
Buzzer Test 6 Instrument name and software version are displayed, press OK.
Buzzer Test
7 The date of the last service is displayed, press OK.
8 A test of the instrument buzzer is performed, press OK.

1
Not always pre-programmed. Depends on customer-specific contract.
Calib Press Offset

ÄKTAdesign Pump
Calib Press P-900 User Manuall 18-1120-04 Edition AE
Offset 47
01234567 YM 012345
Pump P-900
V1.00
B Reference information
Date of Maintenance
?
Buzzer Test
B.1.5 Calibrate Pressure Offset

1 Ensure that the pump is at zero pressure.

Calib Press Offset
3 Select sub menu Calib Press Offset, press OK. “Working, please wait ...” is
displayed until the calibration is completed.
B.2 Setup menu

B.2.1 Set pressure limit
Sets high and low pressure limits. When the limit is reached the pump
is set in Pause mode, a buzzer sounds and an error message Overpressure
(on ascending pressure) or Underpressure (on descending pressure) is
shown. The message has to be confirmed by pressing OK.
OK.
Setup Hi Press Limit 1 Select sub menu Setup Hi Press Limit, press OK. OK.
OK.
Setup Hi Press Limit
Setup Hi Press Limit 2 Set the value, press OK. OK.
Setup Lo Press Limit
Setup
Setup
Hi Press Limit
Lo Press Limit 3 Repeat step 1 and 2 for Setup Lo Press Limit.
Setup Lo Press Limit B.2.2 Set pressure full scale on recorder

Sets the full scale pressure for the chart recorder output. The full scale value
will correspond to 1 V output on the analog output.
Setup Pr. Full Scale 1 Select sub menu Setup Pr. Full Scale, press OK.
Setup Pr. Full Scale
Setup Pr. Full Scale 2 Set the value, press OK.
B.2.3 Set pressure unit
Sets the pressure unit used in the display.
Setup Pressure Unit
Setup Pressure Unit 1 Select sub menu Setup Pressure Unit, press OK.
Setup Pressure Unit
Setup Pressure Unit

2 Select either MPa, bar or psi, press OK.
B.2.4 Set gradient base

Setup Gradient Base
Sets the base for the gradient to either time or volume.
Setup Gradient Base
Setup Gradient Base
1 Select sub menu Setup Gradient Base, press OK.
Setup Gradient Base
2 Select either time or volume, press OK.

B.2.5 Set gradient mode

Sets one-pump gradient (1), two-pump gradient (2) or double (D) flow rate
mode. The one-pump mode is used for low pressure gradient formation
with switch valve SV-903. Two-pump mode is only available with both A and
B pumps installed, and varies the speed of each pump for high pressure
gradient formation. Double mode allows for double max flow, and is only
possible with both A and B pump modules installed. In double mode, the two
pump modules are working in parallel with Conc. %B locked to 50%.
1 Select sub menu Setup Gradient Mode, press OK.

Setup Gradient Mode
2 Select either 1,2 or D, press OK.
Note: Selecting one pump gradient mode disables the B pump module.
Gradient mixing is not possible in double mode.
B.2.6 Set pump type

Sets the size of pump modules used for pump A and B, either 100 ml/min or
10 ml/min.
1 Select sub menu Setup Pump-A Type, press OK.

Setup Pump-A Type
2 Select either 100 ml or 10 ml, press OK.
Setup Pump-B Type 3 Select sub menu Setup Pump-B Type, press OK.
4 Select either 100 ml or 10 ml, press OK.
B.2.7 Set compression compensation

A compensation function can be selecteded, to compensate for flow losses
due to compression at high pressure. When the compensation function is
selected, the pump takes into consideration the stated compressability for
the liquid, at the actual pressure. For methanol at 25 MPa, for example, the
loss in flow is reduced from 10% to 1%. However, since this compensation
may affect the reproducability, it should only be used when exact flow rate
is required. A compressability value between 4.0 x 10-10 Pa-1– 15.0 x 10-10 Pa-1
can be stated. Some common values are given below:
Liquid Value in Pa-1

Water 4.6 x 10-10
Methanol 12.1 x 10-10
Most organic solvents (±15%) ˜ 11 x 10
-10
Note: The compensation function is only available for P-903 using 10 ml

pump heads.

1 Select main menu Setup, press OK.
Setup Compr. comp 2 Select sub menu Setup Compr. comp, press OK.
(off) off on
3 Select On to activate, or Off to deactivate compensation. Press OK.
Setup Comp A
(4.0E-10) 4.0 4 Enter a compensation value between 4.0 and 15.0 for pump module A.
Setup Comp B 5 Enter a compensation value between 4.0 and 15.0 for pump module B.
(4.0E-10) 4.0
B.2.8 Set mixer operation

Sets the condition of the mixer connected to the pump.
1 Select sub menu Setup Mixer, press OK.

Setup Mixer
2 Select either auto, off or on, press OK. ”Auto” means the mixer is running
when the pump is in Run, Pause or Hold mode.
B.2.9 Set valve operation

Sets the condition of any switch valve SV-903 connected to the pump.
1 Select sub menu Setup Valve 1, press OK.

Setup Valve 1
2 Select either off or on, press OK.
IN IN
NC NO NC NO
INA CTIVE A CTIVE
B.2.10 Setup language

Sets the language used in the display.
1 Select sub menu Setup Language press OK.

Setup language
(GB) GB D F E I
2 Select the desired language.
GB = British English
D = German
F = French
E = Spanish
I = Italian

B.2.11 Setup unit number

The unit number is the identification the pump has on the UniNetbus. It
should correspond to the number set in UNICORN for the pump. The number
should be set to 0 if one pump is used. If more than one pump is used they
must all have different identification numbers.:
Setup Unit Number

1 Select sub menu Setup Unit Number, press OK.
2 Select unit number (0–25), press OK.
B.2.12 Setup display angle

The display angle can be set to compensate for different viewing heights.
1 Select main menu Setup, press OK.
2 Select sub menu Set Display Angle, press OK.

Set Display Angle
3 Select viewing angle (->\ Up, ->| Mid or ->/ Down), press OK.
B.3 Alarm timer

You can set the alarm function to either a fixed alarm time or using a count-
down timer. The pump can be started or stopped automatically, or an alarm
can sound, at the set time. It is not possible to set both an alarm time and a
count-down timer. Current values are shown in parentheses.
Alarm/Timer 12:30:52 1 Select main menu Alarm/Timer, press OK.
2 Set the action to take place. Press OK to select action. Buzzer will
Alarm/Timer action generate an audible alarm for 15 s and a message. Run will start the
(Bzz) Buzzer Run End
pump at the set flow rate, End will stop the pump, each generating one
beep and a message.
Set Alarm 12:32:21
(0) 00.00.00 3 Use the sub menu Set Alarm, if you want to set an alarm at a fixed time.
Press OK to enter the time value in the form HH.MM.SS, pressing the OK
button after entering each time unit.
Set Timer
(0) 00.00.00 4 If you want to set a count-down timer, turn the dial to select sub menu
Set Timer. Press OK to enter the countdown value in the form HH.MM.SS,
pressing the OK button after entering each time unit.
Alarm/Timer 12:30:52
(Bzz12:33:00)
5 Press ESC button to return to the Alarm/Timer menu which now shows
the set alarm time or count-down time as BzzHH:MM:SS.
Bzz12:41:29 12:41:49 6 When the alarm time is due or the count-down timer reaches 00:00:00,
!! Alarm time !!
an alert display is shown and the instrument beeps, until the OK button is
pressed.
Set Clock
(00:36:53) 00:36:53

Alarm/Timer off?
(Buzz 05:15:00)
Alarm/Timer 12:30:52
(Bzz12:33:00)
Bzz12:41:29 12:41:49
!! Alarm time !!
Set Clock
The alarm timer is based on the internal instrument clock which can be set
(00:36:53) 00:36:53 in the Set Clock menu placed after the Alarm/Timer menu. The clock will be
reset when power is turned OFF.
Alarm/Timer off? An already set alarm/timer function can be reset by pressing OK in the
(Buzz 05:15:00)
menu Alarm/Timer off?
B. 4 Service displays
The instrument has service displays for use by authorised service personnel.
Enter Access Code!
If the service display Enter Access Code: is accidentally selected, press the
ESC-button to exit to the normal operation display.

B.5 Menu overview

End 1.00ml/min Run 1.00ml/min
Pause 1.00ml/min
Run 23.40ml/min Hold 1.00ml/min

2.00MPa 45.5%B
Set Flow Rate
Set Conc./Gradient Set Concentration B
Set Gradient Length
Set Gradient Target
Check Check Heads 23.0MPa
Check Total Run Time
Check Piston Strokes
Check Service Mode Telephone Service

012345678901
Calib Press Offset Contract Number
012345678901
Serial Number
01234567 YM 012345
Pump P-900
V1.00
Date of Maintenance
?
Setup Setup Hi Press Limit
Buzzer Test
Setup Pressure Unit
Setup Gradient Base
(time) time volume
Setup Gradient Mode
Setup Pump-A Type
Setup Pump-B Type

Setup Compr. comp
(off) off on
Setup Comp A
(4.0E-10) 4.0
Setup Comp B
Alarm/Timer 12:30:52 Alarm/Timer action (4.0E-10) 4.0
(Bzz) Buzzer Run End Setup Mixer
Set Alarm 12:32:21
(0) 00.00.00 Setup Valve 1
Set Timer Setup language
(0) 00.00.00
Set Clock Setup Unit number
(00:36:53) 00:36:53
Alarm/Timer off? Set Display Angle
(Buzz 05:15:00)


Reference information C
C Technical specifications
C.1 Operating data
The specifications are valid only at pressures higher than 0.2 MPa.
Flow rate range

P-901;
isocratic mode 0.01–100 ml/min in steps of 10 µl/min
gradient mode 0.01–100 ml/min in steps of 10 µl/min
double mode; 0.01–200 ml/min in steps of 10 µl/min
P-903;
isocratic mode 0.001–10 ml/min in steps of 1 µl/min
gradient mode 0.001–10 ml/min in steps of 1 µl/min
double mode; 0.001–20 ml/min in steps of 1 µl/min
Pressure range
P-901 0–10 MPa (100 bar, 1450 psi)
P-903 0–25 MPa (250 bar, 3625 psi)
pH stability range 1–13, 1–14 (<1 day exposure)
Viscosity Max. 5 cP
Flow rate accuracy
P-901;
0.2–10.0 MPa ±2% or 20 µl/min whichever is greater
P-903;
0.2–25.0 MPa ±2% or 2 µl/min whichever is greater, with
compression compensation activated
Flow rate reproducibility
P-901;
Flow rate ≥0.5 ml/min rsd < 0.5%
P-903;
Flow rate ≥0.05 ml/min rsd < 0.5%
Gradient composition
P-901;
accuracy <±1% at 0.5–100 ml/min
reproducibility rsd <0.25% at 0.5–100 ml/min
P-903;
accuracy <±1% at 0.05–10 ml/min
reproducibility rsd <0.25% at 0.05–10 ml/min
Pressure sensor
Range 0–27.5 MPa
Offset error <0.05 MPa
Scale error <±2%
Environment +4 to +40 °C
20–95% relative humidity
84–106 kPa (840–1060 mbar)

c Reference information
C.2 Physical data

Delay volumes
Total volume between inlet
and outlet (per pump module)
P-901 <800 µl/module
P-903 <600 µl/module
Inlet- and outlet tubing UNF 10-32 2B ”Fingertights” with capillary
tubing 1/16” outer diameter
Control Stand alone or from a PC running UNICORN version
2.20 or higher, through UniNet 1 cable connection.
Degree of protection
Housing IP 20
Wetted materials
Piston Aluminium oxide
Pump head Titanium alloy
Pump seal;
P-901 PE (polyethylene) and stainless steel (Elgiloy)
P-903 PTFE (polytetrafluoroethylene) and stainless steel
(Hastelloy)
Check valve PTFE (polytetrafluoroethylene), PVDF (polyvinylidene-
fluoride), titanium and ruby/sapphire.
Output manifold PEEK, Kalrez and stainless steel (Hastelloy)
Chemical resistance The wetted parts are resistant to organic solvents
and salt buffers commonly used in chromatography
of biomolecules, except 100% Ethylacetate,
100% Hexane, and 100 % Tetrahydrofuran (THF).
Power requirement 100–240 V AC, 50–60 Hz
Power consumption Up to 400 VA including accessories
Digital input 5 V TTL low or contact closure (see section A.1 for
pin significance)
Digital output TTL, open collectors
Recorder output 0–1 V full scale
Functions Languages selectable; English, German, Spanish,
French, Italian
Display 2 rows with 20 characters each
Dimensions, H x W x D 150 x 260 x 370 mm
Weight 17 kg

Reference information C
Compliance with standards The declaration of conformity is valid for the

instrument only if it is:
• used in laboratory locations
• used in the same state as it was delivered
from GE Healthcare except for alterations
described in the User Manual
• connected to other CE labelled GE Healthcare
modules or other products as recommended.
Safety standards This product meets the requirement of the
Low Voltage Directive (LVD) 73/23/EEC
through the following harmonized standards:
• EN 61010-1
• IEC 61010-1
• CAN/CSA-C22.2 No. 61010-1
• UL61010-1
EMC standards This device meets the requirements of the
EMC Directive 89/336/EEC through the
following harmonized standards:
• EN 61326 (emission and immunity)
• EN 55011, GR 2, Class A (emission)
• This device complies with part 15 of the
FCC rules (emission). Operation is subject
to the following two conditions:
1 This device may not cause harmful interference.
2 This device must accept any interference
received, including interference that may cause
undesired operation.

C Reference information

Reference information D
D Accessories and spare parts

Item Quantity per pack Code no.
P-901 Stand-alone 1 18-1114-00
P-901 System mounted 1 18-1108-56
Seal kit in4cluding 4 piston seals and
4 rinse membranes:
Seal kit, 100 ml: 1 18-1113-12
Piston kit, 100 ml 1 18-1112-13
Pump head capillaries 100 ml 1 18-1117-52
Rinsing/draining housing 100 ml 1 18-1112-03
Pump head, 100 ml complete 1 18-1128-48
Inlet manifold, 100 ml, UNF 5/16” 1 18-1112-25
P-903 Stand-alone 1 18-3000-00

P-903 System mounted 1 18-3100-00
Seal kit including 4 piston seals and
4 rinse membranes:
Seal kit, 10 ml: 1 18-1120-77
Piston kit, 10 ml 1 18-1120-75
Pump head capillaries 10 ml 1 18-1120-81
Rinsing/draining housing 10 ml 1 18-1120-76
Pump head, 10 ml complete 1 18-1128-47
Inlet manifold, 10 ml, UNF 5/16” 1 18-1120-80
Recorder cable 1 18-1110-64

Check valve kit, including: 1 18-1128-66
1 inlet check valve, 1 outlet check valve
Switch Valve SV-903 1 18-1114-49
Mixer M-925 1 18-1118-89
Mixer chamber 0.6 ml 1 18-1118-90
Mixer chamber 2 ml 1 18-1118-91
Purge valve 1 18-1128-87

O-ring for purge valve 10 19-0036-01
Inlet filter set 10 18-1113-16
Inlet tubing kit, i.d. 1/8’’, o.d. 3/16”, 1 18-1114-40
Inlet tubing kit, i.d. 1.0 mm , o.d. 1/8’’ 1 18-1119-26
Tubing kit for rinsing system 1 18-1113-32
Purge kit 1 18-1124-53
Inlet filter assembly 2 18-1113-15

D Reference information
Item Quantity per pack Code no.

Teflon tubing, i.d. 1/8”, o.d. 3/16” 3m 18-1112-47
Teflon tubing, i.d. 0.063”, o.d. 1/8” 3m 18-1121-16
Tubing connector for 1/8” o.d. tubing 10 18-1121-17
Tubing connector for 3/16” o.d. tubing 10 18-1112-49
Ferrule for 1/8” o.d. tubing 10 18-1121-18
Ferrule for 3/16” o.d. tubing 10 18-1112-48
Stop plug, 5/16” 5 18-1112-50
Stop plug, 1/16” 5 18-1112-52
Union Luer female/1/16” male 2 18-1112-51
Union 1/16” female/M6 male 6 18-1112-57
Union M6 female/1/16” male 8 18-1112-58
PEEK tubing, i.d. 0.50 mm, o.d. 1/16” 2m 18-1113-68
Teflon tubing, i.d. 0.75 mm, o.d. 1/16” 2m 18-1112-54
Fingertight connector 1/16” 10 18-1112-55

Short instructions 6
12 Short instructions
The following short instructions are intended as a guide to users who are
fully familiar with safety precautions and operating instructions described
in this manual. The instructions assumes that the instrument is installed
according to the installation instructions.
P-9
00
Selftest
Pump synchronization
End 0.00ml/min
0.00MPa 0.0%B
1 Switch on the instrument by means of the mains switch on the
rear panel.
Set Flow Rate
2 Press OK to start synchronisation.
End 2.50ml/min 3 The main operating menu is shown.
4 Set the flow rate.

Run 2.50ml/min
2.0MPa 0.0%B 5 Start the pump by setting it in RUN-mode.
6 The main operating menu is shown.

Set Gradient Length
7 Start a gradient by setting the Gradient Length and Gradient Target.

Set Gradient Target
8 Stop the pump by setting it in END-mode.
Run 2.50ml/min

www.chromatography. Drop Design, FPLC, Superloop, UNICORN and ÄKTA are trademarks of
GE Healthcare Ltd, a General Electric Company. GE, imagination at work and
amershambiosciences.com GE monogram are trademarks of General Electric Company.
www.gehealthcare.com Microsoft and Windows are either trademarks or registered trademarks of

Microsoft Corporation in the United States and/or other countries.
All goods and services are sold subject to the terms and conditions of sale
GE Healthcare Bio-sciences AB of the company within GE Healthcare which supplies them. GE Healthcare
Björkgatan 30 reserves the right, subject to any regulatory and contractual approval, if
required, to make changes in specifications and features shown herein, or
751 84 Uppsala discontinue the product described at any time without notice or obligation.
Contact your local GE Healthcare representative for the most current
Sweeden information.
© 2005 General Electric Company – All rights reserved.
GE Healthcare Bio-Sciences AB, a General Electric Company.
GE Healthcare Bio-Sciences AB
Björkgatan 30, 751 84 Uppsala, Sweden
GE Healthcare Europe GmbH

Munzinger Strasse 5, D-79111 Freiburg, Germany
GE Healthcare UK Ltd
Amersham Place, Little Chalfont, Buckinghamhire, HP7 9NA, UK
GE Healthcare Bio-Sciences Corp

800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327, USA
GE Healthcare Bio-Sciences KK
Sanken Bldg. 3-25-1, Huakunincho, Shinjuku-ku, Tokyo 169-0073, Japan
Asia Paci.c Tel: +852 2811 8693 Fax: +852 2811 5251 • Australasia Tel: + 61 2 9899 0999 Fax: +61 2 9899 7511 • Austria Tel: 01/57606-1619 Fax: 01/57606-1627 • Belgium Tel: 0800 73 888
Fax: 03 272 1637 • Canada Tel: 800 463 5800 Fax: 800 567 1008 • Central, East, & South East Europe Tel: +43 1 982 3826 Fax: +43 1 985 8327 • Denmark Tel: 45 16 2400 Fax: 45 16 2424 • Finland &
Baltics Tel: +358-(0)9-512 39 40 Fax: +358 (0)9 512 39 439 • France Tel: 01 69 35 67 00 Fax: 01 69 41 96 77 • Germany Tel: 0761/4903-490 Fax: 0761/4903-405 • Italy Tel: 02 27322 1 Fax: 02 27302
212 Japan Tel: +81 3 5331 9336 Fax: +81 3 5331 9370 • Latin America Tel: +55 11 3933 7300 Fax: +55 11 3933 7304 • Middle East & Africa Tel: +30 210 9600 687 Fax: +30 210 9600 693 • Netherlands
Tel: 0165 580 410 Fax: 0165 580 401 • Norway Tel: 815 65 555 Fax: 815 65 666 • Portugal Tel: 21 417 7035 Fax: 21 417 3184 • Russia & other C.I.S. & N.I.S Tel: +7 (095) 232 0250, 956 1137
Fax: +7 (095) 230 6377 • South East Asia Tel: 60 3 8024 2080 Fax: 60 3 8024 2090 • Spain Tel: 93 594 49 50 Fax: 93 594 49 55 • Sweden Tel: 018 612 1900 Fax: 018 612 1910 • Switzerland
Tel: 0848 8028 12 Fax: 0848 8028 13 • UK Tel: 0800 616928 Fax: 0800 616927 • USA Tel: 800 526 3593 Fax: 877 295 8102
imagination at work
User Manual 18-1120-04 AE

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ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE 5

6 ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE

D Accessories and spare parts

About this manual

ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE 7

8 ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE

• P-901 stand-alone or system mounted

• P-903 stand-alone or system mounted

Fluid inlet tubing

ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE 9

Pump P-900 features:

– Pressure range 0–10 MPa (100 bar, 1450 psi).

– Flow rate range 0.01–100 ml/min isocratic and gradient mode.

– Double mode flow rate range of 0.01–200 ml/min isocratic mode.

– Pressure range 0–25 MPa (250 bar, 3625 psi).

– Flow rate range 0.001–10 ml/min isocratic and gradient mode.

– Double mode flow rate range of 0.001–20 ml/min isocratic mode.

• All pumps have a pressure sensor connected to pump module A.

• Switch-valve control for low pressure gradients and BufferPrep.

These accessories are:

• 2-way Switching Valve SV-903.

10 ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE

• The instrument is designed for indoor use only.

• Do not use in a dusty atmosphere or close to spraying water.

• Do not block the air inlet and outlet of the unit.

WARNING! Always disconnect the power supply before attempting to

WARNING! Incorrectly fitted tubing may loosen, causing a jet of liquid

WARNING! When using hazardous chemicals, all suitable protective

WARNING! The instrument must not be opened by the user. It contains

WARNING! The instrument must be connected to a grounded mains

WARNING! NaOH is injurious to health. Avoid spillage.

WARNING! When using hazardous chemicals, take all suitable protective

WARNING! This is a Class A product. In domestic environment this

ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE 11

12 ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE

CAUTION! The following information should be read carefully to ensure

2.2 General precautions

2.3 Connecting electrical signal cables

WARNING! Pump P-900 must be connected to a grounded mains socket

ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE 13

2.3.1 Connecting to chart recorder (if used)

2.4 Connecting to communication link

CAUTION! The mains power to ÄKTAexplorer and ÄKTApurifier must be

1 Connect two UniNet cables to the UniNet 1 connectors. The instrument

2.5 Connecting to supply voltage

WARNING! The instrument must be connected to a grounded mains

The instrument contains no user replaceable fuse.

WARNING! Only use mains cables delivered or approved by GE

14 ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE

2.6 Installing the piston seal rinsing system

4 Connect a syringe to the rinsing tubing that is connected to the

ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE 15

2.7 Connecting the inlet tubing

Connect the tubing using male 5/16”-connections.

2.8 Purging the pump

2 Connect a male Luer syringe of about 30 ml to the open end of the

6 Repeat steps 3 to 5 for pump module B, if fitted.

16 ÄKTAdesign Pump P-900 User Manuall 18-1120-04 Edition AE