General (B.Pharm VI A+B) - Microsoft Teams

First Sessional Exam.
BP 605T:
Pharmaceutical Biotechnology
(B.Pharm VI A+B)
1. Which among the following chromatographic technique is most commonly used

for enzyme purification
(1 Point)
Ion-exchange
Hydrophobic interaction
Size extrusion
Size exclusion
2. Glucose biosensor is an example of __________________ type of biosensors.

(1 Point)
Optical
Thermal
Amperometric
Conduct metric
3. The first synthetic polymer used for enzyme immobilization is

(1 Point)
Nylon
Polystyrene
Silk
Polyamide
4. _________________ biosensors are based on the principle of acoustics and are

also called as acoustic (sound waves) biosensors.
(1 Point)
Conduct metric
Calorimetric
Piezoelectric
Optical
5. The extra chromosomal, self replicating, circular DNA molecules present in

bacterial cells are known as
(1 Point)
Plasmid
Vector
All of the given options
rDNA
6. Which of the following is NOT a physical method of immobilization?

(1 Point)
None
Adsorption
Microencapsulation
Entrapment
7. Which bacteria is used for the development of nodules in cereal crops?
(1 Point)
Urokinase
Rhizobium
Clostridium
Lipase
8. Biological recognition element(s) or bioreceptors used in biosensor is / are

(1 Point)
Antibodies
Microorganisms
Enzymes
9. Which of the following is a characteristic of electrochemical biosensor?

(1 Point)
Detects the movement of electrons between electrodes
Detects the change in light adsorption
Detects the number of photons emitted out
Detects the angle at which electrons are emitted
10. Cutting of DNA molecules into segments is done by

(1 Point)
Urokinase
Ligase
Isomerase
Restriction endonucleases
11. The term ‘biotechnology’ was coined by __________ in __________year.

(1 Point)
Karl Ereky and 1919
Arthur Konberg and 1917
Arthur Konberg and 1912
Karl Ereky and 1905
12. Genetic engineering technique is used to produce human insulin from non-
human source. Identify the microorganism used to produce the same.
(1 Point)
Bacillus subtilis
Saccharomyces cerevisiae
Clostridium botulinum
Mycoplasma haemofelis
13. 1,4-α-D-glucan maltohydrolase is another name for

(1 Point)
γ- Amylase
ß- Amylase
α- Amylase
None
14. White biotechnology explains the applications related to which of the following
fields?
(1 Point)
Industrial
Aquatic
Agricultural
Medical
15. Conversion of C3 plants into C4 plants to increase the photosynthetic rate can
be done through
(1 Point)
Protoplast fusion
Recombinant DNA technology
Both
None
16. Type of biosensors used to detect urinary tract infections are

(1 Point)
Thermometric
Amperometric
Piezoelectric
Luminescent
17. Production of human growth hormones is an example of which type of

biotechnology?
(1 Point)
Blue
Red
White
Green
18. Which of the following statement is INCORRECT for a biosensor?
(1 Point)
The linearity of the sensor should be high
Chemical interference must be minimized for obtaining the correct results
Value of electrode response per substrate concentration should be low
Response time should be low
19. Enzyme immobilization is a process of confining the enzyme molecules to a

(1 Point)
Solid support
Reaction mixture
Liquid support
Semi solid support
20. Who is known as the Father of Biosensors?

(1 Point)
Karl Ereky
Arthur Konberg
Watson and Crick
Leland C Clark
21. Optimum pH for α-Amylase is

(1 Point)
6.7–7.0
4.0–5.0
5.5-6.5
22. The correct sequence of gene manipulation for rDNA is
P. Generation and selection of DNA fragments

Q. Introduction of the recombinant vectors into host cells
R. Expression of the gene to produce the desired product
S. Multiplication and selection of clones containing the recombinant molecules
T. Insertion of the selected DNA into a cloning vector
(1 Point)
P-Q-T-R-S
P-Q-R-S-T
P-T-Q-S-R
P-T-Q-R-S
23. Which of the following aspect is not included in Gene cloning strategies in
relation to recombinant DNA technology
(1 Point)
Insertion of DNA fragments into host cells
Generation of desired DNA fragments
Selection or screening of the recipient cells for the recombinant DNA molecules
Introduction of the vectors into host cells
24. The process used to create same genetic replica of another organism is known
as
(1 Point)
rDNA
Cloning
DNA finger printing

Gene mapping
25. The most commonly employed cross linked polymer used for enzyme
entrapment is
(1 Point)
EC
Collodion
Polyarylamide
Polyacrylamide Gel
26. Which statement among the following is CORRECT?

(1 Point)
Both Proteases and Peptidases are endocellular
Proteases are extracellular and Peptidases are endocellular
Proteases are endocellular and Peptidases are extracellular
Both Proteases and Peptidases are extracellular
27. Enzyme catalyzed joining of DNA fragments is called as

(1 Point)
Gene splicing
Gene mapping
Gene cloning
Gene sequencing
28. Using genetic technique in forensic science is also called as

(1 Point)
Gene Therapy
In-vivo Culture
Genetic Finger Printing
Hybridoma Technology
29. ______________ is an immobilized enzyme is used for the production of High

fructose corn syrup.
(1 Point)
Glucose isomerase
Aspartase
Glucose hydrolygase
Nitrile hydratase
30. Gene transfer can be achieved by which of the following method(s)

(1 Point)
Particle bombardment
Plasmid
micro-injection
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First Sessional Exam.

1. Which among the following chromatographic technique is most commonly used

2. Glucose biosensor is an example of __________________ type of biosensors.

3. The first synthetic polymer used for enzyme immobilization is

4. _________________ biosensors are based on the principle of acoustics and are

5. The extra chromosomal, self replicating, circular DNA molecules present in

All of the given options

6. Which of the following is NOT a physical method of immobilization?

8. Biological recognition element(s) or bioreceptors used in biosensor is / are

All of the given options

9. Which of the following is a characteristic of electrochemical biosensor?

Detects the movement of electrons between electrodes

Detects the change in light adsorption

Detects the number of photons emitted out

Detects the angle at which electrons are emitted

10. Cutting of DNA molecules into segments is done by

11. The term ‘biotechnology’ was coined by __________ in __________year.

Karl Ereky and 1919

Arthur Konberg and 1917

Arthur Konberg and 1912

Karl Ereky and 1905

13. 1,4-α-D-glucan maltohydrolase is another name for

Recombinant DNA technology

16. Type of biosensors used to detect urinary tract infections are

17. Production of human growth hormones is an example of which type of

The linearity of the sensor should be high

Chemical interference must be minimized for obtaining the correct results

Value of electrode response per substrate concentration should be low

Response time should be low

19. Enzyme immobilization is a process of confining the enzyme molecules to a

Semi solid support

20. Who is known as the Father of Biosensors?

Watson and Crick

21. Optimum pH for α-Amylase is

22. The correct sequence of gene manipulation for rDNA is

P. Generation and selection of DNA fragments

Insertion of DNA fragments into host cells

Generation of desired DNA fragments

Introduction of the vectors into host cells

DNA finger printing

26. Which statement among the following is CORRECT?

Both Proteases and Peptidases are endocellular

Proteases are extracellular and Peptidases are endocellular

Proteases are endocellular and Peptidases are extracellular

Both Proteases and Peptidases are extracellular

27. Enzyme catalyzed joining of DNA fragments is called as

28. Using genetic technique in forensic science is also called as

Genetic Finger Printing

29. ______________ is an immobilized enzyme is used for the production of High

30. Gene transfer can be achieved by which of the following method(s)

All of the given options

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11. The term ‘biotechnology’ was coined by in year.