BIRLA INSTITUTE OF TECHNOLOGY & SCIENCE, PILANI

WORK INTEGRATED LEARNING PROGRAMMES

COURSE HANDOUT

Academic Term FIRST SEMESTER 2020-2021

Course Title Environmental Sampling and Analytical Methods

Course ID No. SSTM ZG516

Lead Instructor DR. ABHISHEK SURESH DHOBLE

Instructor(s) PROF. SMITA RAGHUVANSHI

Course Modules

Module Module Title Objectives

No

1 Structure and  To learn the structure and properties of pollutants.

function of  Function of the pollutants in environment, and biological
pollutants, and
impact.
analysis
 Able to distinguish the classes of organic and inorganic
pollutants. Interpret analytical data.

2 Spectrochemical  Able to solve the problems from various spectroscopic

Analysis of methods for pollutant analysis by :
pollutants  UV-Visible,
 Fluorescence ,
 IR,
 AAS,
 Fast spectroscopic methods.
3 Mass Spectrometry  To learn analysis of air and water and soil samples by
of pollutants  EI mass spectrometry,
 ESI mass spectrometry
 MALDI mass spectrometry
 To review the trends in monitoring pollutants in water
sources by mass spectrometry
4 Analysis of  To learn analysis by separation methods based on
pollutants by  HPLC
Separation methods  GC
 LC-MS/MS of pollutants in water

SSTM ZG516 (ES&AM) First Semester 2020-21 Page 1 of 9

 5 Chemical  To learn basic equilibrium in chemical systems and
equilibrium and electrochemical process
electroanalytical  Electrochemical methods: Potentiometry, Cyclic
methods for voltammetry
analysis of  Electrochemical detection methods
pollutants

Text Book(s)

T1 Fundamentals of Analytical Chemistry, SkooG, West, Holler, Crouch, 9th Edition,

ISBN-10, 8131522695, Cengage Learning India Pvt Ltd, New Delhi. (for all modules)

T2 Spectroscopy, Donald L. Pavia, 4th Edition, Cengage Learning India Pvt Ltd, New
Delhi
T3 Principles of Environmental Chemistry, Second Edition, James E. Girard, Jones &
Bartlett Learning , (For module 1)

Reference Book(s) & other resources

R1 Research Papers relevant to the topic will be provided by the instructor during the
course (Other reference material will be shared dynamically in eLearn and/or in the
classes. Students are advised to remain updated!)

R2 Analytical Chemistry, Gary D. Christian, Sixth Edition, ( Available in BITS, GOA,

libraray)

R3 Gerard Kiely, Environmental Engineering, McGraw Hill Education, Special Indian

Edition, 2007

Self-Study & Contact Session Plan

Module Title: Structure and function of pollutants, and analysis.

Session 1

SSTM ZG516 (ES&AM) First Semester 2020-21 Page 2 of 9

Topic Topic Title Text book/ Reference
No.

1.1 Structural formula T1, T3, R1 and R3

1.2 Functional groups T1, T3, R1 and R3

1.3 Organic pollutants T1, T3, R1 and R3

1.4 Inorganic pollutants T1, T3, R1 and R3

Module Title: Structure and function of pollutants, and analysis.

Session 2

Topic Topic Title Text book/Reference

No.

2.1 Source of organic pollutants: T1, T3, R1 and R3

2.2 Source of Inorganic pollutants: T1, T3, R1 and R3

2.3 Common Analytical methods T1, T3, R1 and R3

2.4 Sampling methods T1, T3, R1 and R3

Module Title: Structure and function of pollutants, and Sampling and analytical data.
Session 3

Topic Topic Title Text book/ Reference

No.

3.1 Analytical data collection T1 and R2

3.2 Errors in chemical analysis T1 and R2

3.3 Standardisation and Calibration T1 and R2

Module Title: Spectro-chemical Analysis of pollutants

Session 4

Topic Topic Title Text book/Reference

No.

SSTM ZG516 (ES&AM) First Semester 2020-21 Page 3 of 9

 4.1 Interaction of Radiation and Matter T1 and R1

4.2 Spectroscopy and discovery of elements T1 and R1

4.3 Radiation absorption T1 and R1

4.4 Why is red solution red? T1 and R1

Module Title: Spectrochemical Analysis of pollutants

Session 5

Topic Topic Title Text book/ Reference

No.

5.1 Optical instrumentation in analysis T1 and R1

5.2 Detectors T1 and R1

5.3 UV-Vis spectrometer T1 and R1

5.4 IR spectrometer T1 and R1

Module Title: Spectrochemical Analysis of pollutants

Session 6

Topic Topic Title Text book/ Reference

No.

6.1 UV-Vis absorption analysis of pollutants T1 and T2

6.2 Automated methods T1 and T2

6.3 IR analysis of pollutants T1 and T2

6.4 Functional group identification T1 and T2

Module Title: Mass Spectrometry of pollutants

Session 7

Topic Topic Title Text book/Reference

No.

7.1 Molecular Fluorescence T1 and R1

SSTM ZG516 (ES&AM) First Semester 2020-21 Page 4 of 9

 7.2 Detecting the pollutants T1 and R1

7.3 Chemiluminescence Methods T1 and R1

Module Title: Mass Spectrometry of pollutants

Session 8

Topic Topic Title Text book/ Reference

No.

8.1 Electron ionisation(EI) of pollutants T1 and T2

8.2 EI instrumentation T1 and T2

8.3 EI fragmentations T1 and T2

An optional Review Session before Mid-Semester Test (will be announced as and when need arises)

Module Title: Mass Spectrometry of pollutants

Session 9

Topic Topic Title Reference

No.

9.1 ESI mass spectrometry of pollutants T2 and R1

9.2 ESI mechanism T2 and R1

9.3 ESI fragmentation T2 and R1

9.4 Monitoring pollutants in water by ESI , and MALDI T2 and R1

mass spectrometry

Module Title: Analysis of pollutants by Separation methods

Session 10

Topic Topic Title Reference

No.

10.1 Separation by precipitation T1 and R1

10.2 Separation by distillation T1 and R1

SSTM ZG516 (ES&AM) First Semester 2020-21 Page 5 of 9

 10.3 Extraction method T1 and R1

10.4 Ion exchange T1 and R1

Module Title: Analysis of pollutants by Separation methods

Session 11

Topic Topic Title Reference

No.

11.1 Chromatographic separations T1 and R1

11.2 Chromatogram T1 and R1

11.3 Plate and Plate height T1 and R1

11.4 Column specifications T1 and R1

Module Title: Analysis of pollutants by Separation methods

Session 12

Topic Topic Title Reference

No.

12.1 HPLC instrumentation T1 and R1

12.2 HPLC methods T1 and R1

12.3 Sampling methods T1 and R1

12.4 Detectors for detecting the pollutants T1 and R1

Module Title: Chemical equilibrium and electroanalytical methods for analysis of pollutants
Session 13

Topic Topic Title Reference

No.

13.1 Determining Standard potentials experimentally T1 and R1

13.2 Constructing redox titration curves T1 and R1

13.3 Potentiometric end points T1 and R1

SSTM ZG516 (ES&AM) First Semester 2020-21 Page 6 of 9

Module Title: Chemical equilibrium and electroanalytical methods for analysis of pollutants
Session 14

Topic Topic Title Reference

No.

14.1 Oxidising and reducing agents T1 and R1

14.2 Applying standard reducing potentials T1 and R1

14.3 Determination of Chromium species in water samples T1 and R1

Module Title: Chemical equilibrium and electroanalytical methods for analysis of pollutants
Session 15

Topic Topic Title Reference

No.

15.1 Potentiometery T1 and R1

15.2 Reference electrodes T1 and R1

15.3 Instrumentation T1 and R1

15.4 Potentiometric titrations- equilibrium constant T1 and R1

Module Title: Chemical equilibrium and electroanalytical methods for analysis of pollutants
Session 16

Topic Topic Title Reference

No.

16.1 Voltammetry T1 and R1

16.2 Instrumentation T1 and R1

16.3 Sampling and Cyclic voltammetry T1 and R1

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 16.4 Clarks Oxygen Sensor , Determination of Dissolved T1 and R1
oxygen

An optional Review Session before Comprehensive Exam (will be announced as and when need
arises)

Evaluation Scheme:

Legend: EC = Evaluation Component; AN = After Noon Session; FN = Fore Noon Session

No Name Type Duratio Weight Day, Date, Session, Time

n

EC-1 Assignment / Quiz Online - 15% To be announced

EC-2 Mid-Semester Test Closed 2 hours 35% Sunday, 11/10/2020 (FN)

Book 10 AM - 12 Noon

EC-3 Comprehensive Exam Open 3 hours 50% Sunday, 29/11/2020 (FN)

Book 9 AM – 12 Noon

Syllabus for Mid-Semester Test (Closed Book): Topics in Session Nos. 1 to 8

Syllabus for Comprehensive Exam (Open Book): All topics (Session Nos. 1 to 16)
Important links and information:

Elearn portal: https://elearn.bits-pilani.ac.in

Students are expected to visit the Elearn portal on a regular basis and stay up to date with the latest
announcements and deadlines.
Contact sessions: Students should attend the online lectures as per the schedule provided on the
Elearn portal.
Evaluation Guidelines:

1. EC-1 consists of either two Assignments or three Quizzes. Students will attempt them
through the course pages on the Elearn portal. Announcements will be made on the portal, in
a timely manner.

2. For Closed Book tests: No books or reference material of any kind will be permitted.

3. For Open Book exams: Use of books and any printed / written reference material (filed or
bound) is permitted. However, loose sheets of paper will not be allowed. Use of calculators
is permitted in all exams. Laptops/Mobiles of any kind are not allowed. Exchange of any
material is not allowed.

SSTM ZG516 (ES&AM) First Semester 2020-21 Page 8 of 9

It shall be the responsibility of the individual student to be regular in maintaining the self study
schedule as given in the course handout, attend the online lectures, and take all the prescribed
evaluation components such as Assignment/Quiz, Mid-Semester Test and Comprehensive Exam
according to the evaluation scheme provided in the handout.

SSTM ZG516 (ES&AM) First Semester 2020-21 Page 9 of 9