Colorimeter and Fully Auto Analyzer - Group H
Colorimeter and Fully Auto Analyzer - Group H
Colorimeter and Fully Auto Analyzer - Group H
Light source
FILTER:
Three Types:
1.Prism
2.Grating
PRISM
•When light travels from one medium to another
medium , it is refracted and enters in the new medium
at a different angle.
Prism wavelength spectrum
GLASS FILTER:-
1.Glass
3.Plastic cuvette
• Shorter Life Span
•Easily get Scratches
•Low Cost
PHOTOCELL (PHOTODETECTOR)
•These are the devices to measure the
intensity of light by converting light energy in to
electric energy.
•They are made up of light sensitive material
such as selenium.
GALVANOMETER
• Readout device.
• A galvanometer is used to detect and measure
electrical current produced by the
photodetector.
ADVANTAGE:-
DISADVANTAGES:-
• Less sensitive.
• Limited range of filters are available.
• If the light source is not stable ,there is a
possibility of errors due to a change from the
initial light intensity during a measurement.
• The manual operation are limited.
Spectrophotometer
Principle
The working of colorimeter & Spectrometers is
based on Beer's & Lambert's law.
O.D.=2 – log T%
Differences: Colorimeter &
Spectrophotometer
Colorimeter Spectrophotometer
Limited for the visible
Ultra violet & infrared
portion of spectrum region also visible
(visible light) e.g.340nm
Cheap
Very costly
Two digit reading after
Four digits reading after
desimal point. desimal point .
Less sensitive
More sensitive
Glass are used.
Prism are used.
Glass cuvette or test
Quartz cuvette is used
tube is used for reading which does not absorb
which absorb 340nm 340nm light.
light.
Halogen lamps are used.
Tungsten lamps are
Can use specific filter.
used. • Can do kinetic method.
Can’t use specific filter.
Can’t do kinetic method.
Auto analyzers are mainly two types:-
• Semi Auto Analyzer
• Fully Auto Analyzer
Semi Auto Analyzer
Example : ERBA CHEM 5- PLUS
Advantage :
Displaying the test results
Printing & memorizing these results
Graphs of all linear & nonlinear reactions.
Disadvantage :
initial stage of Analysis are performed manually
Pipetting of reagent
Pipetting of specimen
Mixing & incubation.
This instrument require minimum 500 microliters of reagent for test.
Manual L-J chart to draw
Fully Auto Analyzer
The auto analyzer perform all the function of semi
auto analyzer.