VUGENE’s Post

We are ready to chat with you about #Fluidfm, #CellEDIT and #Liveseq at the #Latsis Symposium today!

🔬 Scientists introduce #MP3seq, a revolutionary method for measuring #ProteinProteinInteractions at a massive scale. This highly quantitative yeast two-hybrid approach can analyze over 100,000 interactions, potentially accelerating cell and gene therapy research. 🎯 MP3-seq uses DNA barcodes to directly measure protein interaction strength. Researchers applied it to study rationally designed heterodimers and investigate specificity in coiled-coil interactions, advancing our understanding of protein networks. 💡 The team combined MP3-seq with #AlphaFoldMultimer to predict and measure interactions. While AI models show potential for prescreening, experimental validation remains crucial for the quantitative ranking of interaction strengths. Quick Read: https://lnkd.in/gkgdQufK #Bioinformatics #ProteinScience #MolecularBiology #AIinBiology #StructuralBiology #ScienceNews

Attending SFN in Chicago and need help generating CRISPR models? Generating knockout and variant specific models in iPSCs can be tricky. Perhaps we can help so that you don't have to spend more time generating iPSC models than meaningful data. Meet Travis Hardcastle, Scott Messenger and Nano Schmidt below to learn more. #SFN24 #Neuroscience2024 #iPSC #CRISPR #NIH #GeneEditing

"Enzymatic DNA Synthesis: Going to Great Lengths" The demand for DNA is at an all-time high, driven by fields such as cell and gene therapy, vaccine development, protein engineering, data storage, and biomanufacturing. To meet these demands, researchers are developing innovative ways to customize and mass-produce synthetic DNA, sidestepping the limitations and low yields of extracting it from natural sources. Read the full story @ https://lnkd.in/gAkPwK3v #DNA #chemical_syngthesis #enzymastic_synthesis #chiralpedia

Learn all about #GeneTherapy thanks to this course based on the ADVANCE curriculum: https://lnkd.in/g2jQc_pc

🆕 #MERSCOPE Publication: Insights into Facioscapulohumeral Muscular Dystrophy (FSHD) 🧬 The latest study from UC Irvine uncovers that FSHD is linked to the abnormal de-repression of the transcription activator DUX4, affecting only a small percentage of cells. Traditional RNA-seq methods struggle to analyze multinucleated myotubes, but #MERFISH offers a breakthrough with its subcellular resolution capabilities. This study provides comprehensive gene network profiling of multinucleated cells and identifies FSHD-induced transcriptomic changes during myoblast differentiation. Discover how the study using #MERFISH is transforming our understanding of FSHD at the molecular level. 📖 Read the full paper here: https://hubs.ly/Q02Bmtmm0 #FSHD #SpatialTranscriptomics #MERFISH #DUX4 #Myotubes #MuscularDystrophy #Vizgen #ResearchPublication

Announcing the next CryoChats™, Wednesday December 4th:  The Last 100 Yards: Cell and Gene Therapy Clinical Considerations! Registration is now open: https://lnkd.in/gUXVHa83 CryoChats™ brings together thought leaders and practitioners in cryopreservation with the aim of advancing the industry through collaboration. In the first 30 minutes, each panelist will be interviewed. During the second half-hour, attendees are encouraged to connect, ask questions, and make introductions using Zoom's chat feature. Let's move the industry forward together! Featuring world-renowned Cryobiologist Allison Hubel, PhD, President of the Society for Cryobiology Hosted by: Jeffrey D. Allen | Chairman & CEO at Evia Bio Panelist: TBA Dominic Clarke, Marty Giedlin, PhD, Jeffrey D. Allen, John Baust, Marc J. Centrella, Scott Fisher, John Bonham-Carter, Mike O'Mara, Priya Baraniak, Ph.D., Russ Straate, Aby J. Mathew, PhD, Adam Joules, Bill Ward, Narayan Bhagroo, Austin Bigley, Robyn Osborne, MS, Carmine Carpenito, Dalip Sethi, PhD, MBA, Mike O'Mara, Laura Pierce #Cryopreservation #Biotechnology #ResearchAndDevelopment #CellTherapy #EviaBio #celltherapy #LifeScience

Fanzor2, a eukaryotic CRISPR-Cas homolog, characterized using advanced cryo-electron microscopy. This breakthrough, led by Elizabeth Kellogg and team at St. Jude Children's Research Hospital, could revolutionize gene editing by providing new insights into the mechanisms of CRISPR systems in eukaryotes. Really excited about this as it will be adding to the toolbox we may use for therapeutic applications. #CRISPRCas9 #GeneEditing #structuralbiology

BMKMANU S1000 Spatial Transcriptomics Project Experience - Olfactory Bulb in Mouse Brain Using single-cell level spatial transcriptome technology, BMKMANU S1000, the olfactory bulb sample in the mouse brain was detected, obtaining a total of transcripts and spatial positional information of 17,564 cells. With the help of BMKMANU cell segmentation technology, the cell types of the olfactory bulb sample were accurately identified, and the spatial distribution of the main olfactory bulb nerve layer, glomerular layer, external plexiform layer, internal plexiform layer, mitral valve layer, granule layer, and subependymal zone was reconstructed. Number of segmented cells: 17,564  Median gene count at single-cell resolution: 1142 Contact us now to talk about your project requirements: https://lnkd.in/dEwG33Jd #Spatial Transcriptomics #BMKGENE #BMKMANU S1000 #single cell level

Groundbreaking study unveils chromatin remodelers' complex behaviors! In a new #eLife publication, researchers from Johns Hopkins University and Howard Hughes Medical Institute have uncovered distinct activities of chromatin remodelers. These key proteins modify chromatin architecture, enabling regulatory proteins to access condensed genomic DNA, thereby controlling gene expression. Employing the advanced dynamic single-molecule tool, C-Trap, the team delivered the first real-time, detailed observations of how these proteins interact with DNA and nucleosomes. This significant discovery enhances our understanding of genome regulation and offers valuable insights for developing therapeutic strategies targeting remodeling pathways. Discover more: https://bit.ly/3Le9q8d