Biomarker (BMKGENE) Europe

This pool was quite a fun challenge! Targeted sequencing is used to confirm the integration of inserts at target locations in CRISPR experiments, while whole genome sequencing is commonly used to identify off-target modifications. This approach offers a comprehensive overview of the genome, enabling researchers to identify unintended changes effectively. #ScienceFans #ScienceChallenge #ScienceCommunity

FPKM (Fragments Per Kilobase of transcript per Million mapped reads) is a commonly used unit for quantifying gene expression in the gene expression quantification analysis of RNA sequencing data. FPKM values can indicate the expression level of genes in samples, considering the effects of gene length and sequencing depth. When calculating FPKM values, the following steps are typically involved:  1. Calculate gene expression: Initially, determine the number of reads for each gene from the RNA-Seq sequencing data.  2. Normalization: Normalize the number of reads for each gene by dividing by the total number of mapped reads, calculated per million, to consider differences in sequencing depth between samples.  3. Consider gene length: Genes with longer lengths may capture more reads. Divide the normalized reads by the gene length in kilobases to obtain the number of reads per kilobase per million mapped reads. FPKM values can be used to compare the expression levels of different genes within the same sample or across different samples and to observe changes in gene expression under different conditions. Due to its consideration of gene length and sequencing depth, FPKM is widely used in the quantitative analysis of RNA-Seq data. With technological advancements, some studies use alternative units such as TPM (Transcripts Per Million) to represent gene expression levels. #BMKGENE can provide reliable transcriptome sequencing and professional bioinformatics analysis services for your research needs. #RNA #Transcriptome #GeneExpressionQuantification #FPKM

🔍 Ready for a challenge? Dive into today’s #BMKGENEQuiz and let your science smarts shine! #ScienceChallenge #ScienceCommunity

In the article published in Theranostics, Klotho-derived peptide 1 inhibits cellular senescence in the fibrotic kidney by restoring Klotho expression via posttranscriptional regulation. Klotho deficiency is a common feature of premature aging and chronic kidney disease (CKD). Restoring Klotho's expression could be a logical strategy for protecting against various nephropathies. This study demonstrates that KP1, a Klotho-derived peptide, inhibits cellular senescence and induces Klotho expression via posttranscriptional regulation mediated by miR-223-3p and lncRNA-TUG1. By restoring endogenous Klotho, KP1 elicits a broad spectrum of protective actions and could serve as a promising therapeutic agent for fibrotic kidney disorders. #BMKGENE provided small RNA sequencing and bioinformatics analysis services for this study. #miRNA #Klotho #CellularSenescence #KidneyFibrosis

In sequencing projects, a bioinformatician primarily analyzes sequencing reads and identifies genetic variants. This involves processing raw data, aligning reads and detecting mutations, which are essential steps in whole genome sequencing (WGS) projects. Another common type of sequencing project is RNA-seq, where, after reading alignment, gene expression levels are quantified to study transcriptomic profiles. #ScienceChallenge #ScienceCommunity

🌟 Wrapping up an incredible experience at the Inaugural Conference of the European Society for Spatial Biology (ESSB) e.V. in Berlin! From engaging talks to vibrant networking sessions, the event brought together passionate minds shaping the future of #SpatialBiology. Highlights from the conference: 📸 Connecting with experts at our booth, sharing insights into cutting-edge genomic services. 📸 A packed auditorium buzzing with innovative ideas and discussions. Thank you to everyone who stopped by our booth and visited our incredible team, Dr. Arina Urazova, Jie Xu, and Menglong, as we explored the potential of this transformative field! #SpatialBiology #ESSB2024 #BiomedicalResearch #Innovation #Networking #ScienceForTheFuture

Another Success Story in BMKGENE's Genomics Database Construction! Exciting news today as our client's groundbreaking work was published in the prestigious journal Nucleic Acids Research, showcasing their achievement in establishing the "MolluscDB 2.0", a cutting-edge functional and evolutionary genomics database encompassing over 1400 molluscan species. This exceptional database integrates a wealth of over 4200 multi-omics data resources across various mainstream omics dimensions, including high-quality genomes, bulk transcriptomes, single-cell transcriptomes, proteomes, epigenomes, and microbial metagenomes. The molluscan multi-omics resources cover 92% of the eight orders and 76 families within the Mollusca phylum, spanning diverse environments from land, freshwater, and nearshore to deep-sea regions. Furthermore, it offers a range of tools for genomic comparative analysis. BMKGENE was pivotal in establishing this groundbreaking international molluscan comprehensive genomics database, MolluscDB. In recent years, the growth of functional genomics data has been exponential, underscoring the importance of systematic integration and in-depth analysis of vast multi-omics resources. Customizing analysis platforms tailored to species-specific characteristics makes efficient utilization of omics data achievable, providing robust support for gene resource exploration and genetic breeding efforts. #BMKGENE has successfully collaborated to construct genomics databases for multiple species, boasting a wealth of experience in this domain. If you're considering consolidating genomic data for your species of interest, we welcome you to contact us for more information! #BioinformaticsDatabase #MultOmics #BigData #Genomics

🔬 Take on today’s #BMKGENEQuiz and show us what you’ve got! #ScienceFans #ScienceChallenge #ScienceCommunity

Wide-targeted metabolomics in plants combines the advantages of non-targeted high-resolution and broad coverage techniques with the sensitivity and precise quantification capabilities of targeted multiple reaction monitoring (MRM) methods. By utilizing high-resolution mass spectrometry for comprehensive secondary scan coverage of all metabolites, followed by accurate identification through a vast high-resolution secondary spectrum database, specific MRM ion pairs are extracted to establish sample-specific libraries. Subsequently, triple quadrupole mass spectrometry is employed for precise quantification. This integrated approach offers numerous technical advantages, including extensive substance identification, precise qualitative and quantitative analysis, wide dynamic range, high sensitivity, and excellent reproducibility. #BMKGENE provides services in plant-wide-targeted metabolomics, non-targeted metabolomics, and targeted metabolomics, offering a highly efficient method for detecting complex plant metabolites. #PlantMetabolomics #MetabolomicsServices

Excited about the future of Spatial Biology? Join us at the Conference of the European Society for Spatial Biology (ESSB) 🔬 Why Spatial Biology? Spatial biology delves into the intricate relationships between cells within tissues, integrating insights from medicine, biology, physics, and computer science to revolutionize our understanding of health and disease. From groundbreaking spatial technologies to interdisciplinary collaboration, this field is shaping the future of biomedical research. Don't miss this chance to explore innovations, connect with experts, and shape the future of this exciting field! 📌 Visit us at booth Nr. 18 #SpatialBiology #BiomedicalScience #ESSB2024 #Innovation #Networking #Research