BIA Separations Inc.’s Post

AAT Bioquest Helixyte™ iFluor® Nucleic Acid Labeling #Dyes enable the precise attachment of fluorophores to nucleic acids with ease and efficiency resulting in high-yield production of bright, stable #fluorescence probes for applications like #blotting or #ISH.  https://lnkd.in/e6_nYeWj #biosciences #lifesciences #nucleicacidlabelling #PCR #DNA #RNA #oligonucleotides #microarray

A great read about hydrophilic liquid chromatographic (HILIC) of intact proteins or glycoproteins subunits. HILIC has been gaining popularity for the analysis of complex biological macromolecules including oligosaccharides and oligonucleotides but also glycoproteins either as their intact forms or their partially digested subunits (Middle-up/down). A growing number of high-selectivity, wide-pore hydrophilic sorbents are now available from nano- to narrowbore formats making possible the analysis/separation of complex and heterogeneous protein isoforms featured with numerous post-translational modifications such as glycosylation, glycation, acetylation, oxidation, phosphorylation and clipping. https://lnkd.in/e2F-gSAe

What a nice review paper! Even after a decade of pushing for better and better protein #HILIC methods, I still smile when I see a beautiful subunit glycoform separation in print 😊 Here’s to improving capabilities for larger and larger oligos and nucleic acids ! Thank you for sharing Valegh Faid, PhD. Congratulations on the nicely written and summarized piece of work, Sara Tengattini, gabriella. massolini, Francesca Rinaldi, ENRICA CALLERI and Caterina Temporini! #HPLC

NEW: Enhance the performance of your oligonucleotides with Locked Nucleic Acid and Minor Groove Binder / Elipse Dark Quencher (MGB:EDQ) modifications. Dual-Labeled Probes labeled with Minor Groove Binder / Eclipse Dark Quencher (MGB:EDQ) strongly bind to the minor groove of B-form DNA. This leads to better qPCR performance by enhancing SNP detection via mismatch discrimination. #oligos #ASO #MGB #qPCR

📢 New oligonucleotide application note using HILIC The application note from Waters Corporation discusses the use of GTxResolve Premier BEH Amide 300 Å 1.7 μm columns for Hydrophilic Interaction Chromatography (HILIC) to analyze oligonucleotides. 💡Here are the key points: ❗ Objective: To demonstrate the benefits of using GTxResolve Premier BEH Amide columns for both denaturing and non-denaturing analyses of oligonucleotides up to 100 nucleotides in length. 📄 Method: HILIC is used to facilitate oligonucleotide analysis without ion pairing reagents, improving chromatographic performance. ✅ Findings: The columns provide excellent retention time reproducibility, peak symmetry, and size-dependent retention behavior for oligonucleotides ranging from 20 to 100 mers. ⬆ Benefits: Enhanced resolution of longer oligonucleotides, robust chromatography, and the ability to investigate single-stranded RNA impurities in siRNA under various conditions. 🔗 Link to the app note can be found in the comments below Feel free to ask if you need more details or have any specific questions! 😊

Enclosing hydrazine into a cycle creates aza-analogues of piperidines, pyrrolidines, and azepines. These structures can enhance molecular polarity, provide accessible sites for chemical functionalization, and allow for fine-tuning of structural and electronic properties of biomolecules. For example, RMC-9805, a KRAS G12D inhibitor, contains a piperazic acid moiety and has recently entered Phase 1 clinical trials. Enamine offers a collection of cyclic and bicyclic saturated hydrazine structures: https://bit.ly/4ey7DI3 Try our cyclic hydrazines in your research!

Explore EM-seq capabilities to support your methylome studies. This method improves upon current methyl-seq solutions, like WGBS, enabling higher sequencing quality & sensitivity. Connect with an expert to learn how EM-seq can support your next project: https://hubs.ly/Q02YdLGB0 #methylationanalysis #NGS #EMseq #WGBS #methylseq #epigenetics

Determining the purity of LNPs with nucleic acids is challenging due to size variations. We present AUC methods to differentiate between empty LNPs and various RNA loading states. You will learn: 1. Grasp the difficulties in assessing LNPs' purity due to their varying sizes. 2. Discover AUC methods using multiwavelength and D2O for measuring LNPs' properties. 3. Learn how density matching helps differentiate empty LNPs from those with RNA based on density. 📅 Save the Date: Thursday, April 18 ⏰ 10:00 am EST | 4:00 pm CET 🔗 Register here: https://lnkd.in/eV-VPQnD If you can't attend the webinar due to time zone differences and would like the meeting minutes, please let me know in the chat box. #LNP #AUC

New Product Spotlight: Helixyte™ Green, Optimized for quantitative real-time PCR (qPCR) Dealing with the presence of contaminating RNA or ssDNA? AAT Bioquest scientists have developed Helixyte Green helping researchers optimize the qPCR performance. It has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher) and the same spectral properties of PicoGreen® (PicoGreen® is the trademark of Invitrogen). But the particular formulation of Helixyte Green makes it much greater sensitive to dsDNA, thus especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. https://lnkd.in/g-DrKw6U #qpcr #geneexpression #crispr #newproduct

Ion exchange chromatography is an ideal technique to purify biomolecules with high productivity. As the target substances become larger, the demands on the process resins are changing. YMC has developed a new and innovative resin to target large biomolecules and particles such as adeno-associated viruses (AAVs), protein complexes and plasmid DNA (pDNA): MacroSep IEX Q!  This new strong anion exchange resin offers an optimized macro-porous structure that ensures an efficient purification of these molecules. With its high binding capacity for large particles and molecules and high separation efficiency even at elevated flow rates, MacroSep IEX Q is a perfect addition to DSP platforms. https://lnkd.in/gJemjjVh #ymc #chromatography #liquidchromatography #anionexchange #AAV #biomolecules #DBC #ionexhangechromatography