BIA Separations Inc.

[Improved performance] From theory to practice. 📔➡️🧑🔬 See how NC State BTEC enhanced their AAV purification with the PATfix platform! 🔎 They integrated the PATfix platform for simultaneous analyses, improving purification methods like size exclusion chromatography (SEC) and steric exclusion chromatography. 𝐎𝐩𝐭𝐢𝐦𝐢𝐬𝐚𝐭𝐢𝐨𝐧 𝐚𝐭 𝐡𝐚𝐧𝐝 PATfix’s inline pH and conductivity sensors optimized gradient monitoring, enhancing AAV purification efficiency and effectiveness. Read on: https://lnkd.in/eFxk79VN #casestudy #AAV #purification #PATfix #biotech

[New study] Lentiviral vectors are being increasingly used as tools for stable integration of large gene inserts into the genomes of both dividing and non-dividing cells. 𝐑𝐞𝐜𝐞𝐧𝐭 𝐚𝐧𝐢𝐦𝐚𝐥 𝐬𝐭𝐮𝐝𝐢𝐞𝐬 𝐡𝐚𝐯𝐞 𝐬𝐮𝐜𝐜𝐞𝐬𝐬𝐟𝐮𝐥𝐥𝐲 𝐝𝐞𝐦𝐨𝐧𝐬𝐭𝐫𝐚𝐭𝐞𝐝 𝐭𝐡𝐞𝐢𝐫 𝐮𝐬𝐞 𝐢𝐧-𝐯𝐢𝐯𝐨 To enable more widespread use, as well as development of in-vivo therapies in humans, better downstream processes for lentiviral purification are needed. In this work, we present the results of lentiviral downstream optimisation on CIM® QA monolith with the use of DoE. 💡DoE enabled an increase in infectious lentiviral recovery to 70%. This is a 3x increase, compared to initial results. Read on:  https://lnkd.in/djRP-jNi #invivo #purification #genetherapy #donwstream #biotechnology

[MSS2024] Did you miss MSS2024? No worries, we’ve got you covered. 💻 Join your chosen MSS2024 lectures on demand and watch the content (again) at your convenience. All available lectures are divided into three streams: 1️⃣nucleic acids stream 2️⃣general aspects of purification technologies stream 3️⃣viruses, vlps, and nanoparticles stream Visit the page below to see the full list of lectures and grab your early-bird registration for this on-demand content untill December 31st! ✏️ Register here: https://lnkd.in/dzgnqVPg #MSS2024 #MSS2026 #ondemand #purification #biotech #biotechnology

[Join us live] 📅Join us on December 4th on our webinar where Luka Bevc will talk about optimizing lentiviral purification and enhancing chromatography with monoliths. 💡Lentiviral vectors (LVVs) can effectively deliver up to 10 kb of target genes and can infect non-dividing cells, making them a popular vector choice for cell and gene therapies. 🚫But a major drawback of using LVV is its arduous purification process. This webinar describes a systematic approach to using monoliths for LVV purification, including using monoliths in a multi-well plate format with a DOE approach, for faster and parallel testing. ✏️ Register here: https://lnkd.in/dDVKk9YW #purification #lenitiviral #monoliths #biotech #DOE 

[Improved performance] Neutralized E. coli cell lysate is a complex intermediate sample in the production process pDNA for use in vaccines and gene therapy. 🚫  But some remains can interfere with different analytical techniques, used for lysate characterization, thus decreasing method accuracy, sensitivity and/or robustness. 𝐁𝐞𝐭𝐭𝐞𝐫 𝐝𝐞𝐬𝐚𝐥𝐭𝐢𝐧𝐠 𝐟𝐨𝐫 𝐛𝐞𝐭𝐭𝐞𝐫 𝐚𝐜𝐜𝐮𝐫𝐚𝐜𝐲 💡 In our study, we present the use of an innovative method for desalting of E. coli cell lysate using monolithic spin columns* and compare its performance with traditional centrifugal filters. Read on to see how monolithic spin columns ensure rapid desalting of E. Coli cell lysate for CGE analysis: https://lnkd.in/dgxHcGUK   *𝘛𝘩𝘦 𝘮𝘰𝘯𝘰𝘭𝘪𝘵𝘩𝘪𝘤 𝘴𝘱𝘪𝘯 𝘤𝘰𝘭𝘶𝘮𝘯𝘴 𝘥𝘪𝘴𝘤𝘶𝘴𝘴𝘦𝘥 𝘪𝘯 𝘵𝘩𝘪𝘴 𝘴𝘵𝘶𝘥𝘺 𝘢𝘳𝘦 𝘯𝘰𝘵 𝘺𝘦𝘵 𝘢𝘷𝘢𝘪𝘭𝘢𝘣𝘭𝘦 𝘧𝘰𝘳 𝘤𝘰𝘮𝘮𝘦𝘳𝘤𝘪𝘢𝘭 𝘴𝘢𝘭𝘦. #genetherapy #pDNA #monoliths #purification #biotech

[The world of mRNA] Learn how we’ve shortened the process of production and purification of tRNA from approximately a day and a half to 6 hours! ⏱️ 💡 Our study also demonstrated how at-line HPLC monitoring can be used to monitor, and improve, production yields of IVT-derived tRNAs. 𝐅𝐫𝐨𝐦 𝐦𝐑𝐍𝐀 𝐭𝐨 𝐭𝐑𝐍𝐀 Though the IVT monitoring approach is now widely used for mRNA applications, we show that adjustments in HPLC peak interpretation are required to apply the methodology to tRNA. Read on: https://lnkd.in/dAqxqaAG #mRNA #tRNA #puirifcation #IVT #HPLC #biotech

[Product close-up] 𝐓𝐫𝐲 𝐨𝐮𝐫 𝐏𝐀𝐓𝐟𝐢𝐱 𝐝𝐞𝐦𝐨! PATfix software is designed to handle multidetector data processing and helps you extract maximum information from your samples 👉 it  offers quick and powerful tools for advanced post-analysis chromatography, data evaluation and calculations. 💡 This modern web application offers simple data import&management, it eases collaboration with your team and clients, all while ensuring top security and regulatory compliance. ✏️ For a detailed understanding of the PATfix system’s capabilities, we invite you to access our demo software: https://lnkd.in/disZNDds #PATfix #demo #purification #chromatography #biotech 

[Improved performance] Chromatographic purification of pDNA is often a bottleneck due to low dynamic binding capacities (DBC), pDNA recovery and/or low selectivity of columns. 𝐇𝐨𝐰 𝐜𝐚𝐧 𝐰𝐞 𝐢𝐧𝐜𝐫𝐞𝐚𝐬𝐞 𝐃𝐁𝐂? 🔎 The goal of our investigation was to increase the DBC for pDNA on a monolith stationary phase by grafting the surface with linear polymethacrylate brushes, while retaining high pDNA recovery and chromatographic selectivity between pDNA and RNA impurities. 𝐔𝐩 𝐭𝐨 8-𝐭𝐢𝐦𝐞𝐬 𝐥𝐚𝐫𝐠𝐞𝐫 𝐃𝐁𝐂 Dynamic binding capacities for 7.3 kpb pDNA were up to 8-times larger compared to non-grafted CIMmultus 1 ml columns. And that’s not all … read on: https://lnkd.in/dHmgrGxy #pDNA #chromatography #RNA #purification #biotech

[Join us live] 📅Join us on November 19th for a live webinar titled Process in the product: a new analytical tool for AAV production optimization and monitoring. Blaž Bakalar and Ivana Petrovic Koshmak, PhD will explore the determination of percentage of full capsids in complex samples, including lysed harvests and spent media, with rapid analysis (results provided within 25 minutes per sample) and baseline separation of empty and full capsids. Join us to: ✔ gain insights into how the at-line analytics provides precise and reliable data that can inform critical decisions in bioprocessing, leading to more effective and efficient production strategies ✔ discover the practical application of the at-line analytical monitoring in bioreactor runs, specifically in the context of AAV8 production in HEK suspension cells, and how it can be implemented into your existing processes to achieve better results ✔ learn how leverage optimization of upstream processes through Design of Experiments (DoE) by utilizing PATfix AAV Switcher Empty/Full capsid data ✔ gain a comprehensive understanding of the PATfix AAV Switcher, including its capabilities and operational framework ✏️ Register here: https://lnkd.in/dj_6gBPZ #AAV #designofexperiments #PATfix #biotechnology #upstream

[New study] By modifying pH, NaCl, MgCl2 and Arginine concentrations, using 96-well format and DoE, purification conditions can be optimised. 🔎 𝗟𝗲𝗻𝘁𝗶𝘃𝗶𝗿𝗮𝗹 𝗱𝗼𝘄𝗻𝘀𝘁𝗿𝗲𝗮𝗺 𝗽𝗿𝗼𝗰𝗲𝘀𝘀 𝗼𝗽𝘁𝗶𝗺𝗶𝘀𝗮𝘁𝗶𝗼𝗻 Currently, lentiviral based therapies are primarily ex-vivo, such as CAR- T, however recent animal studies have successfully demonstrated their use in-vivo as well. 💡 In this study, we present the results of lentiviral downstream optimisation on CIM® QA monolith with the use of DoE. 𝗧𝗵𝗲 𝗿𝗲𝘀𝘂𝗹𝘁? Optimised conditions from QA monolithic 96-well plates were successfully transferred to CIMmultus QA 1 mL Monolithic Column, where 70% average infectious recovery was achieved. Read on: https:/https://lnkd.in/dGQS3h32 #monoliths #DoE #purification #downstream #biotechnology