Pedro Silva

Groove is a sensation of movement or wanting to move when we listen to certain types of music; it is central to the appreciation of many styles such as Jazz, Funk, Latin, and many more. To better understand the mechanisms that lead to the sensation of groove, we explore the relationship between groove and systematic microtiming deviations. Manifested as small, intentional deviations in timing, systematic microtiming is widely considered within the music community to be a critical component of… Meer weergeven

Groove is a sensation of movement or wanting to move when we listen to certain types of music; it is central to the appreciation of many styles such as Jazz, Funk, Latin, and many more. To better understand the mechanisms that lead to the sensation of groove, we explore the relationship between groove and systematic microtiming deviations. Manifested as small, intentional deviations in timing, systematic microtiming is widely considered within the music community to be a critical component of music performances that groove. To investigate the effect of microtiming on the perception of groove we synthesized typical rhythm patterns for Jazz, Funk, and Samba with idiomatic microtiming deviation patterns for each style. The magnitude of the deviations was parametrically varied from nil to about double the natural level. In two experiments, untrained listeners and experts listened to all combinations of same and different music and micro-timing style and magnitude combinations, and rated liking, groove, naturalnes s, and speed. Contrary to a common and frequently expressed belief in the literature, systematic microtiming led to decreased groove ratings, as well as liking and naturalness, with the exception of the simple short-long shuffle Jazz pattern. A comparison of the ratings between the two listener groups revealed this effect to be stronger for the expert listener group than for the untrained listeners, suggesting that musical expertise plays an important role in the perception and appreciation of microtiming in rhythmic patterns. Minder weergeven

This paper presents work done on classification, segmentation and chronological prediction of cinematic sound employing support vector machines (SVM) with sequential minimal optimization (SMO). Speech, music, environmental sound and silence, plus all pair wise combinations excluding silence, are considered as classes. A model considering simple adjacency rules and probabilistic output from logistic regression is used for segmenting fixed-length parts into auditory scenes. Evaluation of the… Meer weergeven

This paper presents work done on classification, segmentation and chronological prediction of cinematic sound employing support vector machines (SVM) with sequential minimal optimization (SMO). Speech, music, environmental sound and silence, plus all pair wise combinations excluding silence, are considered as classes. A model considering simple adjacency rules and probabilistic output from logistic regression is used for segmenting fixed-length parts into auditory scenes. Evaluation of the proposed methods on a 44-film dataset against k-nearest neighbor, Naive Bayes and standard SVM classifiers shows superior results of the SMO classifier on all performance metrics. Subsequently, we propose sample size optimizations to the building of similar datasets. Finally, we use meta-features built from classification as descriptors in a chronological model for predicting the period of production of a given soundtrack. A decision table classifier is able to estimate the year of production of an unknown soundtrack with a mean absolute error of approximately five years. Minder weergeven

Imprinted genes are expressed primarily or exclusively from either the maternal or paternal allele, a phenomenon that occurs in flowering plants and mammals. Flowering plant imprinted gene expression has been described primarily in endosperm, a terminal nutritive tissue consumed by the embryo during seed development or after germination. Imprinted expression in Arabidopsis thaliana endosperm is orchestrated by differences in cytosine DNA methylation between the paternal and maternal genomes as… Meer weergeven

Imprinted genes are expressed primarily or exclusively from either the maternal or paternal allele, a phenomenon that occurs in flowering plants and mammals. Flowering plant imprinted gene expression has been described primarily in endosperm, a terminal nutritive tissue consumed by the embryo during seed development or after germination. Imprinted expression in Arabidopsis thaliana endosperm is orchestrated by differences in cytosine DNA methylation between the paternal and maternal genomes as well as by Polycomb group proteins. Currently, only 11 imprinted A. thaliana genes are known. Here, we use extensive sequencing of cDNA libraries to identify 9 paternally expressed and 34 maternally expressed imprinted genes in A. thaliana endosperm that are regulated by the DNA-demethylating glycosylase DEMETER, the DNA methyltransferase MET1, and/or the core Polycomb group protein FIE. These genes encode transcription factors, proteins involved in hormone signaling, components of the ubiquitin protein degradation pathway, regulators of histone and DNA methylation, and small RNA pathway proteins. We also identify maternally expressed genes that may be regulated by unknown mechanisms or deposited from maternal tissues. We did not detect any imprinted genes in the embryo. Our results show that imprinted gene expression is an extensive mechanistically complex phenomenon that likely affects multiple aspects of seed development. Minder weergeven

Eukaryotic cytosine methylation represses transcription but also occurs in the bodies of active genes, and the extent of methylation biology conservation is unclear. We quantified DNA methylation in 17 eukaryotic genomes and found that gene body methylation is conserved between plants and animals, whereas selective methylation of transposons is not. We show that methylation of plant transposons in the CHG context extends to green algae and that exclusion of histone H2A.Z from methylated DNA is… Meer weergeven

Eukaryotic cytosine methylation represses transcription but also occurs in the bodies of active genes, and the extent of methylation biology conservation is unclear. We quantified DNA methylation in 17 eukaryotic genomes and found that gene body methylation is conserved between plants and animals, whereas selective methylation of transposons is not. We show that methylation of plant transposons in the CHG context extends to green algae and that exclusion of histone H2A.Z from methylated DNA is conserved between plants and animals, and we present evidence for RNA-directed DNA methylation of fungal genes. Our data demonstrate that extant DNA methylation systems are mosaics of conserved and derived features, and indicate that gene body methylation is an ancient property of eukaryotic genomes. Minder weergeven

Cytosine methylation silences transposable elements in plants, vertebrates, and fungi but also regulates gene expression. Plant methylation is catalyzed by three families of enzymes, each with a preferred sequence context: CG, CHG (H = A, C, or T), and CHH, with CHH methylation targeted by the RNAi pathway. Arabidopsis thaliana endosperm, a placenta-like tissue that nourishes the embryo, is globally hypomethylated in the CG context while retaining high non-CG methylation. Global methylation… Meer weergeven

Cytosine methylation silences transposable elements in plants, vertebrates, and fungi but also regulates gene expression. Plant methylation is catalyzed by three families of enzymes, each with a preferred sequence context: CG, CHG (H = A, C, or T), and CHH, with CHH methylation targeted by the RNAi pathway. Arabidopsis thaliana endosperm, a placenta-like tissue that nourishes the embryo, is globally hypomethylated in the CG context while retaining high non-CG methylation. Global methylation dynamics in seeds of cereal crops that provide the bulk of human nutrition remain unknown. Here, we show that rice endosperm DNA is hypomethylated in all sequence contexts. Non-CG methylation is reduced evenly across the genome, whereas CG hypomethylation is localized. CHH methylation of small transposable elements is increased in embryos, suggesting that endosperm demethylation enhances transposon silencing. Genes preferentially expressed in endosperm, including those coding for major storage proteins and starch synthesizing enzymes, are frequently hypomethylated in endosperm, indicating that DNA methylation is a crucial regulator of rice endosperm biogenesis. Our data show that genome-wide reshaping of seed DNA methylation is conserved among angiosperms and has a profound effect on gene expression in cereal crops. Minder weergeven

Parent-of-origin-specific (imprinted) gene expression is regulated in Arabidopsis thaliana endosperm by cytosine demethylation of the maternal genome mediated by the DNA glycosylase DEMETER, but the extent of the methylation changes is not known. Here, we show that virtually the entire endosperm genome is demethylated, coupled with extensive local non-CG hypermethylation of small interfering RNA–targeted sequences. Mutation of DEMETER partially restores endosperm CG methylation to levels found… Meer weergeven

Parent-of-origin-specific (imprinted) gene expression is regulated in Arabidopsis thaliana endosperm by cytosine demethylation of the maternal genome mediated by the DNA glycosylase DEMETER, but the extent of the methylation changes is not known. Here, we show that virtually the entire endosperm genome is demethylated, coupled with extensive local non-CG hypermethylation of small interfering RNA–targeted sequences. Mutation of DEMETER partially restores endosperm CG methylation to levels found in other tissues, indicating that CG demethylation is specific to maternal sequences. Endosperm demethylation is accompanied by CHH hypermethylation of embryo transposable elements. Our findings demonstrate extensive reconfiguration of the endosperm methylation landscape that likely reinforces transposon silencing in the embryo. Minder weergeven