David Moniz

David Moniz

Chilliwack, British Columbia, Canada
683 followers 500+ connections

About

👋 Welcome! I'm a dedicated and versatile Medical Practitioner and Fellow in rural and…

Activity

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Experience

  • Chilliwack General Hospital Graphic

    Chilliwack General Hospital

    Chilliwack, British Columbia, Canada

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    Chilliwack, British Columbia, Canada

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    Chilliwack, British Columbia, Canada

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    Australia

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    Albury, New South Wales, Australia

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    Katherine, Northern Territory, Australia

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    Katherine, Northern Territory, Australia

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    Katherine, Northern Territory, Australia

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    Katherine Northern Territory

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    Katherine Northern Territory

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    Albury, Australia

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    Geelong Victoria

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    Geelong Victoria

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    Warrnambool

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Education

  • UNSW Graphic

    UNSW

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    Activities and Societies: Sigma Chi Fraternity

Licenses & Certifications

Publications

  • Regulation of early neurite morphogenesis by the Na+/H+ exchanger NHE1. *is listed Sin WC et. al. as both authors contributed equal work towards the publication

    Journal of Neuroscience

    The ubiquitously expressed Na+/H+ exchanger NHE1 plays an important role in regulating polarized membrane protrusion and directional motility in non-neuronal cells. Using NGF-differentiated PC12 cells and murine neocortical neurons in vitro, we now show that NHE1 plays a role in regulating early neurite morphogenesis. NHE1 was expressed in growth cones in which it gave rise to an elevated intracellular pH in actively extending neurites. The NHE1 inhibitor cariporide reversibly reduced growth…

    The ubiquitously expressed Na+/H+ exchanger NHE1 plays an important role in regulating polarized membrane protrusion and directional motility in non-neuronal cells. Using NGF-differentiated PC12 cells and murine neocortical neurons in vitro, we now show that NHE1 plays a role in regulating early neurite morphogenesis. NHE1 was expressed in growth cones in which it gave rise to an elevated intracellular pH in actively extending neurites. The NHE1 inhibitor cariporide reversibly reduced growth cone filopodia number and the formation and elongation of neurites, especially branches, whereas the transient overexpression of full-length NHE1, but not NHE1 mutants deficient in either ion translocation activity or actin cytoskeletal anchoring, elicited opposite effects. In addition, compared with neocortical neurons obtained from wild-type littermates, neurons isolated from NHE1-null mice exhibited reductions in early neurite outgrowth, an effect that was rescued by overexpression of full-length NHE1 but not NHE1 mutants. Finally, the growth-promoting effects of netrin-1, but not BDNF or IGF-1, were markedly reduced by cariporide in wild-type neocortical neurons and were not observed in NHE1-null neurons. Although netrin-1 failed to increase growth cone intracellular pH or Na+/H+ exchange activity, netrin-1-induced increases in early neurite outgrowth were restored in NHE1-null neurons transfected with full-length NHE1 but not an ion translocation-deficient mutant. Collectively, the results indicate that NHE1 participates in the regulation of early neurite morphogenesis and identify a novel role for NHE1 in the promotion of early neurite outgrowth by netrin-1.

    Other authors
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Courses

  • Advanced Life Support Level 2

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  • Advanced Trauma Life Support

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Organizations

  • Australian College of Rural and Remote Medicine

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    - Present
  • Australia Medical Association

    Member

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