CELLULAR

DIVISION

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 Cell Division
All cells are derived from pre-
existing cells
New cells are produced for
growth and to replace damaged or
old cells
Differs in prokaryotes (bacteria)
and eukaryotes (protists, fungi,
plants, & animals)

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 Keeping Cells Identical

The instructions for

making cell parts
are encoded in the
DNA, so each new
cell must get a
complete set of the
DNA molecules

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 DNA Replication
DNA must be
copied or Original DNA
strand
replicated
before cell
division Two new,
identical DNA
Each new cell strands
will then have an
identical copy of
the DNA

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Identical Daughter Cells

Two
identical
daughter
cells

Parent Cell

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Chromosomes

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 Prokaryotic Chromosome
 The DNA of
prokaryotes
(bacteria) is one,
circular
chromosome
attached to the
inside of the cell
membrane

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 Eukaryotic Chromosomes
All eukaryotic cells store genetic
information in chromosomes
 Most eukaryotes have between 10 and
50 chromosomes in their body cells
 Human body cells have 46 chromosomes
or 23 identical pairs

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 Eukaryotic Chromosomes
Each chromosome is composed of a
single, tightly coiled DNA molecule
Chromosomes can’t be seen when
cells aren’t dividing and are called
chromatin

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 Compacting DNA into
Chromosomes
DNA is
tightly
coiled
around
proteins
called
histones

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Chromosomes in Dividing Cells
Duplicated
chromosomes are
called
chromatids &
are held
together by the
centromere

Called Sister Chromatids 11

 Karyotype
 A picture of the
chromosomes from a
human cell arranged
in pairs by size
 First 22 pairs are
called autosomes
 Last pair are the
sex chromosomes
 XX female or XY
male

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 Boy or Girl?
The Y Chromosome Decides

Y - Chromosome

X - Chromosome
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Cell Reproduction

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 Types of Cell Reproduction
Asexual reproduction involves a
single cell dividing to make 2 new,
identical daughter cells
Mitosis & binary fission are
examples of asexual reproduction
Sexual reproduction involves two
cells (egg & sperm) joining to make a
new cell (zygote) that is NOT
identical to the original cells
Meiosis is an example
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Cell Division in
Prokaryotes

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 Cell Division in Prokaryotes
 Prokaryotes such as
bacteria divide into 2 Parent
cell
identical cells by the
process of binary
fission Chromosome
 Single chromosome doubles
makes a copy of
itself
 Cell wall forms Cell splits
between the
chromosomes dividing
the cell
2 identical daughter cells 17
 Prokaryotic Cell
Undergoing Binary Fission

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Animation of Binary Fission

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The Cell
Cycle
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Five Phases of the Cell Cycle
G1 - primary growth phase
S – synthesis; DNA replicated
G2 - secondary growth phase
collectively these 3 stages are
called interphase
M - mitosis
C - cytokinesis

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Cell Cycle

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Interphase - G1 Stage

1st growth stage after cell

division
Cells mature by making more
cytoplasm & organelles
Cell carries on its normal
metabolic activities

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 Interphase – S Stage
Synthesis stage
DNA is copied or replicated

Two
identical
copies
of DNA

Original
DNA
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 Interphase – G2 Stage
2nd Growth Stage
Occurs after DNA has been copied
All cell structures needed for
division are made (e.g. centrioles)
Both organelles & proteins are
synthesized

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What’s Happening in Interphase?

What the cell looks like

Animal Cell

What’s occurring

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 Sketch the Cell Cycle

DNA Copied
Cells prepare for
Cells Division
Mature

Daughter
Cells
Cell Divides into
Identical cells
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Mitosis

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 Mitosis
Division of the
nucleus
Also called
karyokinesis
Only occurs in
eukaryotes
Has four stages
Doesn’t occur in
some cells such
as brain cells
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 Four Mitotic Stages

Prophase
Metaphase
Anaphase
Telophase

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 Early Prophase
Chromatin in nucleus condenses to
form visible chromosomes
Mitotic spindle forms from fibers in
cytoskeleton or centrioles (animal)

Nucleolus Cytoplasm

Nuclear Membrane
Chromosomes

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 Late Prophase
Nuclear membrane & nucleolus are
broken down
Chromosomes continue condensing &
are clearly visible
Spindle fibers called kinetochores
attach to the centromere of each
chromosome
Spindle finishes forming between the
poles of the cell
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 Late Prophase

Chromosomes

Nucleus & Nucleolus have disintegrated

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 Spindle Fiber attached to
Chromosome

Kinetochore Fiber

Chromosome
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 Review of Prophase

What the cell

looks like

What’s happening 35
 Spindle Fibers
The mitotic spindle form from the
microtubules in plants and centrioles
in animal cells
Polar fibers extend from one pole of
the cell to the opposite pole
Kinetochore fibers extend from the
pole to the centromere of the
chromosome to which they attach
Asters are short fibers radiating
from centrioles
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Sketch The Spindle

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 Metaphase
Chromosomes, attached to the
kinetochore fibers, move to the center
of the cell
Chromosomes are now lined up at the
equator Equator of Cell

Pole of
the Cell

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 Metaphase

Asters at
the poles

Spindle Chromosomes
Fibers lined at the
Equator

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 Metaphase

Aster

Chromosomes at Equator
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 Review of Metaphase

What the cell looks

like

What’s
occurring
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 Anaphase
Occurs rapidly
Sister
chromatids are
pulled apart to
opposite poles
of the cell by
kinetochore
fibers

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 Anaphase

Sister
Chromatids
being
separated

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 Anaphase Review

What the
cell looks
like

What’s
occurring

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 Telophase
Sister chromatids at opposite
poles
Spindle disassembles
Nuclear envelope forms around
each set of sister chromatids
Nucleolus reappears
CYTOKINESIS occurs
Chromosomes reappear as
chromatin

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Comparison of Anaphase & Telophase

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 Cytokinesis
Means division of the cytoplasm
Division of cell into two,
identical halves called daughter
cells
In plant cells, cell plate forms
at the equator to divide cell
In animal cells, cleavage furrow
forms to split cell

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 Cytokinesis
Cleavage furrow Cell plate in
in animal cell plant cell

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Mitotic Stages

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 Daughter Cells of Mitosis
Have the same number of
chromosomes as each other and as
the parent cell from which they
were formed
Identical to each other, but smaller
than parent cell
Must grow in size to become mature
cells (G1 of Interphase)

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Identical Daughter Cells

What is
the 2n
or
diploid
number?
2

Chromosome number the same, but cells

smaller than parent cell
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Review
of
Mitosis

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Draw & Learn these Stages

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Draw & Learn these Stages

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 Name the Mitotic Stages:
Interphase

Name this?

Telophase Prophase

Name this?

Metaphase
Anaphase

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 Eukaryotic Cell Division
 Used for growth and
repair
 Produce two new cells
identical to the original
cell Chromosomes during
 Cells are diploid (2n) Metaphase of mitosis

Prophase Metaphase Anaphase Telophase Cytokinesis

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 Mitosis Animation
Name each stage as you see it occur?

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Mitosis in Onion Root Tips
Do you see any stages of mitosis?

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Name the Stages of Mitosis:
Early prophase
Early Anaphase Metaphase

Interphase Early
Telophase,
Begin
cytokinesis

Late Late telophase,

Prophase Advanced Mid-Prophase Late
cytokinesis Anaphase
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 Uncontrolled Mitosis
 If mitosis is not
controlled, unlimited
cell division occurs
causing cancerous
tumors
 Oncogenes are special
proteins that
increase the chance
that a normal cell
develops into a tumor
cell
Cancer cells
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 Meiosis
Formation of Gametes
(Eggs & Sperm)

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 Facts About Meiosis
Preceded by interphase which
includes chromosome replication
Two meiotic divisions --- Meiosis
I and Meiosis II
Called Reduction- division
Original cell is diploid (2n)
Four daughter cells produced that
are monoploid (1n)
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 Facts About Meiosis
Daughter cells contain half the
number of chromosomes as the
original cell
Produces gametes (eggs & sperm)
Occurs in the testes in males
(Spermatogenesis)
Occurs in the ovaries in females
(Oogenesis)

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 More Meiosis Facts
 Startwith 46 double stranded
chromosomes (2n)
After 1 division - 23 double
stranded chromosomes (n)
After 2nd division - 23 single
stranded chromosomes (n)
 Occurs in our germ cells that

produce gametes

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Why Do we Need Meiosis?
It is the fundamental basis of
sexual reproduction
Two haploid (1n) gametes are
brought together through
fertilization to form a diploid
(2n) zygote

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Fertilization – “Putting it
all together”
2n = 6

1n =3

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 Replication of Chromosomes
 Replication is the
process of
duplicating a Occurs in
chromosome Interphase
 Occurs prior to
division
 Replicated copies
are called sister
chromatids
 Held together at
centromere
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 A Replicated Chromosome

Gene X

Homologs Sister
(same genes, Chromatids
different alleles) (same genes,
same alleles)

Homologs separate in meiosis I and

therefore different alleles separate.
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Meiosis Forms Haploid Gametes
 Meiosis must reduce the chromosome number by half
 Fertilization then restores the 2n number

from mom from dad child

too
much!

meiosis reduces
genetic content
The
right
number!
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 Meiosis: Two Part Cell
Division
Sister
chromatids
Homologs separate
separate

Meiosis Meiosis
I II

Diploid
Diploid
Haploid
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 Meiosis I: Reduction Division

Nucleus Spindle
fibers Nuclear
Early envelope
Prophase I Late Metaphase
(Chromosome Prophase I Anaphase Telophase I
number I I (diploid)
doubled)

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 Prophase I

Early prophase Late prophase

Homologs pair. Chromosomes condense.
Crossing over Spindle forms.
occurs. Nuclear envelope
fragments.
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Tetrads Form in Prophase I

Homologous chromosomes Join to form a

(each with sister chromatids) TETRAD

Called Synapsis
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 Crossing-Over
 Homologous
chromosomes in
a tetrad cross
over each other
 Pieces of
chromosomes or
genes are
exchanged
 Produces
Genetic
recombination in
the offspring
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Homologous Chromosomes
During Crossing-Over

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 Crossing-Over

Crossing-over multiplies the already huge

number of different gamete types
produced by independent assortment 76
Metaphase I

Homologous pairs
of chromosomes
align along the
equator of the
cell

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Anaphase I

Homologs separate and

move to opposite poles.

Sister chromatids remain

attached at their
centromeres.

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Telophase I

Nuclear envelopes
reassemble.

Spindle disappears.

Cytokinesis divides cell

into two.

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 Meiosis II
Only one homolog of each
Gene X chromosome is present in
the cell.
cell
Sister chromatids carry
identical genetic
information.

Meiosis II produces gametes with

one copy of each chromosome and
thus one copy of each gene.
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 Meiosis II: Reducing
Chromosome Number

Prophase Metaphase
II II Telophase
Anaphase II 4 Identical
II haploid cells

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Prophase II

Nuclear envelope
fragments.

Spindle forms.

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Metaphase II

Chromosomes align
along equator of cell.

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 Anaphase II
Equator

Pole

Sister chromatids
separate and
move to opposite
poles.

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Telophase II

Nuclear envelope
assembles.

Chromosomes
decondense.

Spindle disappears.

Cytokinesis divides
cell into two.
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Results of Meiosis
Gametes (egg & sperm)
form

Four haploid cells with

one copy of each
chromosome

One allele of each gene

Different combinations of
alleles for different
genes along the
chromosome
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Gametogenesis
Oogenesis
or
Spermatogenesis

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 Spermatogenesis
 Occurs in the
testes
 Two divisions
produce 4
spermatids
 Spermatids mature
into sperm
 Men produce about
250,000,000
sperm per day
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Spermatogenesis in the
Testes
Spermatid

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Spermatogenesis

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 Oogenesis
Occurs in the ovaries
Two divisions produce 3 polar bodies
that die and 1 egg
Polar bodies die because of unequal
division of cytoplasm
Immature egg called oocyte
Starting at puberty, one oocyte
matures into an ovum (egg) every 28
days
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Oogenesis in the Ovaries

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 Oogenesis
First polar body
may divide a
(haploid) X
a Polar
bodies
X a die
a X
X
Mitosis Meiosis I Meiosis II
A X (if fertilization
Oogonium occurs) A
(diploid) Primary
X
oocyte
(diploid) A X Ovum (egg) Mature
Secondary A egg
oocyte X
(haploid) Second
polar body
(haploid)
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Comparing
Mitosis and
Meiosis

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 Comparison of Divisions
Mitosis Meiosis
Number of 2
1
divisions
Number of
2 4
daughter cells
Genetically
Yes No
identical?
Chromosome # Same as parent Half of parent

Where Somatic cells Germ cells

When Throughout life At sexual maturity
Growth and
Role Sexual reproduction
repair 95
96