Probiotic

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What is probiotic?

Probiotics are “live microorganism which when administered


in adequate amounts confer a health benefit on the host”.
(FAO and WHO)

Key concepts of probiotic:


• Probiotics (literally “for life”) are friendly bacteria or yeasts, and
are a concept in contrast to antiobiotics.
• Most common probiotic bacteria  Lactobacilli
and
bifidobacteria
• Most common probiotic yeasts  Saccharomyces cerevisiae
• Potential health benefits from probiotics may vary depending on
the type of probiotics consumed.
Lactobacillus
 Lactobacillus a of Gram-positive, non
forming,
is catalasegenus
negative, facultative anaerobic
spore- or
microaerophilic rods  commonly produce lactic acid as
their major metabolite.
 At present, more than 125 Lactobacillus species have been
identified.

Lactobacillus Lactobacillus casei


Lactobacillus
casei strain Shirota
Lactobacillus casei
 L. casei is broadly distributed in nature and isolated from
dairy producs, silage,and the intestinal tracts of various
animals.
 This species is suggested to have a wide range of pH and
temperature, and specifically used for dairy production.

Lactobacillus Lactobacillus casei


Lactobacillus
casei strain Shirota
L. Casei strain Shirota
 In 1930, Dr. Minoru Shirota (Microbiological laboratory of
Kyoto Imperial University’s School) successfully cultured a
bacterial strain able to survive throughout the intestines.

Lactobacillus Lactobacillus casei


Lactobacillus
casei strain Shirota
L. Casei strain Shirota
Scanning
electron
micrograph
(SEM) of the
bacteria

Lactobacillus
casei
Shirota
strain.

Source: https://fineartamerica.com/featured/lactobacillus-casei-shirota-bacteria-
sem-science-photo-library.html
Survivability of L. casei Shirota

Method
Healthy subjects drank
two 65-mL bottles of
fermented milk for 3
weeks. On days 7, 14, 21,
and 28 after subjects
stopped
drinking sample for 7
days, the fecal numbers
of L. casei Shirota were
measured.
Suppression of putrefactive substances

Protein

Experiment Results
The urinary excretion
of 15N, which is a
biomarker of NH3,
and p-[2H4] cresol,
were significantly
A total of 19 healthy lower compared with
volunteers were ingestion of placebo
administered a probiotic
beverage containing 6.5 ×
109 cells of L. casei
Shirota or placebo drink
Potential toxic for 2 weeks twice daily.
metabolites: p-cresol,
ammonia
Preventive effect on colorectal cancer

Results 20% 35%


Experiment reduced reduced
(Ishikawa et al. 2005)
Rice bran

398 Japanese (at the time


free from tumor and who
previously had at L. casei Shirota These results suggested that L. casei
least two colorectal tumors Shirota may prevent development
removed) of colorectal tumors.
How colon cancer is suppressed by LAB?

Read more here: h


ttps://www.scienc
edirect.com/scienc

e/article/pii/S1286
457900003579
How colon cancer is suppressed by LAB?

Read
more
here:
https://ac
ademi
c.ou
p.com/jn/

article/13

0/2/410S/
4686434
Probiotic products
Yogurt
Possibly the most popular probiotic food is live cultured
yogurt or greek yogurt made from the milk of cows, goats
or sheep. Yogurt in most cases can rank at the top of
probiotic foods if it comes from grass-fed animals and has
not been pasteurized.
Source: https://draxe.com/probiotic-foods/

Natto
A popular dish in Japan consisting of fermented
soybeans, natto contains the extremely powerful
probiotic bacillus subtilis, which has been proven
to bolster your immune system, support
cardiovascular health and enhance digestion of
vitamin K2. Also, Natto contains a powerful anti-
inflammatory enzyme called nattokinase that has
been proven to prevent blood clotting
https://draxe.com/probiotic-foods/
Tempe as a probiotic product

Tempe is reported to
contain LAB of 10 million
cells / g. L. fermentum
present in tempe may
serve as probiotic and also
promote cholesterol
absorption.

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