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    (2017 Scheme) : Instrumental Methods of Analysis

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    (2017 Scheme) : Instrumental Methods of Analysis

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    JASMIN T
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    721006 (3)

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    (2017 Scheme) : Instrumental Methods of Analysis

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    JASMIN T

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    © All Rights Reserved
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    of 1

    QP Code: 721006 Reg. No……………………….

    Seventh Semester B. Pharm Degree Special Supplementary


    Examinations July 2022
    Instrumental Methods of Analysis
    (2017 Scheme)
    Time: 3 Hours Max. Marks: 75
     Answer all questions to the point neatly and legibly • Do not leave any blank pages between
    answers • Indicate the question number correctly for the answer in the margin space
     Answer all parts of a single question together • Leave sufficient space between answers
     Draw diagrams wherever necessary
    Essays (2x10=20)

    1. Explain Beer-Lambert’s law. Derive the Beer-Lambert’s equation and explain the
    deviations.
    2. With the help of a schematic diagram, explain the instrumentation of HPLC. Add
    a note on detectors used in HPLC.
    Short Notes (7x5=35)

    3. Explain the different types of spectral shifts observed in UV – visible


    spectroscopy.
    4. Classify sampling techniques in IR spectroscopy. How to prepare solid samples
    by pressed pellet technique to obtain IR spectrum.
    5. Enlist and explain with example the qualitative applications of UV spectroscopy.
    6. What is programmed temperature gas chromatography. Write its principle and
    advantages.
    7. Why high pressure pumps are important in HPLC. Explain the working of
    reciprocating pump.
    8. Define and classify chromatography. Explain the mechanisms of separation in
    chromatography.
    9. Differentiate between free boundary electrophoresis and zone electrophoresis.
    Write the procedure and applications of paper electrophoresis.
    Answer Briefly (10x2=20)
    10.Define quenching of fluorescence.
    11. Differentiate between Hyperchromic and hypochromic effect using UV Spectra.
    12. Why photomultiplier tube is considered as the most sensitive detector in UV
    spectroscopy.
    13. What is the finger print region of IR Spectrum.
    14. Any two applications of gel filtration chromatography.

    15. What are the factors affecting the “Chromatographic column efficiency.
    16. Explain how softening of hard water could be achieved by using “Ion exchange
    chromatography
    17. What is two dimensional development in TLC. Explain its importance.
    18. Explain with examples the specific and non-specific detection techniques in TLC.
    19. Differentiate between normal phase and reversed phase chromatography.
    *******************

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