LC-40D X3 CL Lc-40D XR CL: Instruction Manual

228-97197B
Sep. 2022
Solvent Delivery Module for Shimadzu

Liquid Chromatograph
LC-40D X3 CL
LC-40D XR CL
Instruction Manual
Read this manual thoroughly before you use the product.
Keep this manual for future reference.
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Introduction
Read this Instruction Manual thoroughly before using
the product.
Thank you for purchasing this product.
This instruction manual describes the basic operation, and accessories and options for this
product. Read this manual thoroughly before using the product and operate the product
in accordance with the instructions in this manual.
The following instruction manuals are included with the product in booklet form or in
PDF format. The PDF documentation is on the instruction manual DVD-ROM (Part No.
228-97193-41).
Document Name Document No. Description

Instruction Manual (PDF) 228-97197 This instruction manual.
This manual provides details on how to use
the system:
System Guide (PDF) 228-97194 system performance optimization, analysis
procedure, troubleshooting, validation,
installation, etc.
Safety Guideline Describes the precaution instructions to
228-97195
(Booklet/PDF) ensure safe operation.
Read "Safety Guideline" thoroughly before using the product.

"Safety Guideline" describes the information about the warranty, after-sales service,
safety instructions and precautions to ensure safe operation of the instrument. Keep this
manual for future reference.
Important
• If the user or usage location changes, ensure that this Instruction Manual is always
kept together with the product.
• If this manual or a product warning label is lost or damaged, immediately contact
your Shimadzu representative to request a replacement.
• To ensure safe operation, read the accompanying booklet "Safety Guideline"
before using the product.
• To ensure safe operation, contact your Shimadzu representative if product
installation, adjustment, re-installation (after the product is moved), or repair is
required.
ã 2020-2022 Shimadzu Corporation. All rights reserved.

Original version is approved in English.
LC-40D X3 CL LC-40D XR CL i
Introduction
Notice
• Information in this manual is subject to change without notice and does not represent
a commitment on the part of the vendor.
• Any errors or omissions which may have occurred in this manual despite the utmost
care taken in its production will be corrected as soon as possible, although not
necessarily immediately after detection.
• All rights are reserved, including those to reproduce this manual or parts thereof in
any form without permission in writing from Shimadzu Corporation.
• Nexera and LabSolutions are trademarks of Shimadzu Corporation.
Windows is a registered trademark of Microsoft Corporation in the United States and/or
other countries.
Third-party trademarks and trade names may be used in this publication to refer to
either the entities or their products/services, whether or not they are used with
trademark symbol "TM" or "®".
Indications Used in This Manual

Precaution symbols are indicated using the following conventions:
Indication Meaning
Indicates a potentially hazardous situation which, if not

avoided, could result in serious injury or possibly death.
Indicates a potentially hazardous situation which, if not

avoided, may result in minor to moderate injury or equipment
damage.
Emphasizes additional information that is provided to ensure
the proper use of this product.
The following symbols are used in this manual:
Indication Meaning
Indicates an action that must not be performed.
Indicates an action that must be performed.
Indicates information provided to improve product performance.
Indicates the location of related reference information.
ii LC-40D X3 CL LC-40D XR CL
Electromagnetic Compatibility
Electromagnetic Compatibility
Descriptions in this section apply only to the following models:
• 228-65076-55 LC-40D X3 CL
• 228-65000-55 LC-40D XR CL
This product complies with European standard EN61326, class B for electromagnetic
interference (Emissions) and industrial electromagnetic environment (Immunity).
n EN55011 Emissions (Electromagnetic Interference)
This is a class B product. When this product causes an electromagnetic disturbance to

devices being used near this product, create an appropriate distance between those
devices and this product in order to eliminate the disturbance.
Operating Nexera CL System

n Intended Use
This system is designed to perform qualitative and quantitative analysis of target

compounds in a sample matrix and can be used as a liquid delivery unit of the liquid
chromatograph for general in vitro diagnostic applications. However, only personnel who
have received appropriate training on use of the system can use it for these purposes.
n Calibration
A calibration curve should be generated for each analyte in appropriate methods. At

least four out of six non-zero standards should meet the appropriate criteria, including
the calibration standard lower than the assumed LOQ and the calibration standard at
the highest concentration.
n Quality Control
Implement quality control of the instrument by routinely measuring at least one each of
four types of quality-control samples (a sample with a normal concentration level, a
sample with a concentration level higher than normal as well as a sample lower than
normal, and a blank sample). Check the selectivity/specificity in the matrix used for the
actual sample, the accuracy, correctness, recovery rate, dynamic range, linearity, and lower
limit of quantitation.
Based on these assessment results, check that the results are within the permissible range.
If the assessment results are out of range, the measured data may be invalid, so do not
use analysis results obtained from the instrument in question until it can be confirmed
that it is functioning normally. When, for example, analyzing samples that contain
complex sample matrices such as serum, plasma or urine, it may be possible to obtain
stabilized data by using an appropriate pre-treatment or an internal standard.
LC-40D X3 CL LC-40D XR CL iii

Contents
Introduction Indications Used in This Manual ......................... ii
Electromagnetic Compatibility ............................ iii
Operating Nexera CL System .............................. iii
1 Overview 1.1 Features ............................................................. 1

1.2 Component Parts ................................................. 2
1.3 Optional Parts ..................................................... 3
Parts Identification 7
2 and Function
2.1 Front ..................................................................
2.2 Behind Front Cover ............................................. 8

2.3 Back ................................................................... 9
2.4 Right Side and Bottom ...................................... 10
2.5 Name and Functions of the Operation Panel ....... 11
2.5.1 Display Area ................................................... 13
2.5.2 Operation Area ............................................... 14
3 Operation 3.1 Settings for the Basic Operation ......................... 16

3.1.1 Prior to Key Operation ........................................ 16
3.1.2 Setting Flow Rate ............................................. 16

3.1.3 Setting Compressibility ........................................ 16
3.1.4 Setting Maximum Pressure Limit .............................. 17
3.1.5 Setting Minimum Pressure Limit .............................. 18
3.2 Operation ......................................................... 19

3.2.1 Preparation before Operation ................................. 19
3.2.2 Operation in Constant Flow Delivery Mode .................. 20
3.2.3 Operation in Constant Pressure Delivery Mode .............. 21

3.3 Types of Screens ................................................ 23
3.4 Monitoring Screens ............................................ 24
3.5 Auxiliary Functions Screen (FUNCTION) ................ 28
3.5.1 Auxiliary Functions (FUNCTION) List .......................... 28
3.5.2 [PARAMETER] Setting Group .................................. 31
iv LC-40D X3 CL LC-40D XR CL
Contents
3.5.3 [CONTROL] Setting Group .................................... 37

3.5.4 [SYSTEM] Setting Group ...................................... 38
3.5.5 [UTILITY] Setting Group ....................................... 44
3.5.6 [FILE] Operation Group ........................................ 46
3.6 VP Functions Screen ........................................... 47

3.6.1 List of VP Functions .......................................... 47
3.6.2 Product Information Group (PRODUCT INFO) ................ 49
3.6.3 Maintenance Information Group (MAINTENANCE) ........... 49
3.6.4 Validation Support Information Group (VALIDATION) ........ 53
3.6.5 Calibration Support Information Group (CALIBRATION) ...... 61
3.7 Creating Time Program ...................................... 69

3.7.1 Time Program Command List ................................. 69
3.7.2 Time Program Setting Screen ................................. 70
3.7.3 Creating a Time Program ..................................... 71
3.7.4 Creating a Low-Pressure Gradient Program .................. 73

3.7.5 Deleting a Step ............................................... 74
3.7.6 Starting and Stopping a Time Program ...................... 74
3.7.7 Commands Used for Time Programs Only ................... 75
3.8 Delivery in the Low-Pressure Gradient Mode ....... 76
3.8.1 Set Value ...................................................... 76
3.8.2 Preparation for Delivery ....................................... 76
3.9 Connection with the System Controller or

Workstation ...................................................... 78
3.9.1 Setting the Instrument ........................................ 78
3.9.2 Basic Parameters .............................................. 78
3.10 Connection to External Input/Output
Terminals .......................................................... 78
4 Maintenance 4.1 Periodic Inspection and Maintenance .................. 79

4.1.1 Prior to Inspection and Maintenance ......................... 79
4.1.2 List of Periodic Inspection and Maintenance ................. 82
4.1.3 Check after Inspection and Maintenance .................... 83

4.2 Removing the Right Panel Cover and the Filter
Holder .............................................................. 84
4.3 Removing/Installing the Pump Heads ................... 85
LC-40D X3 CL LC-40D XR CL v
Contents
4.4 Replacing the Plunger Seal ................................ 92

4.4.1 Replacing the Plunger Seal and Backup Ring ................ 92
4.4.2 Resetting the Delivery Amount ............................... 99

4.4.3 Check after Replacement ..................................... 99
4.5 Cleaning/ Replacing the Plunger and
Diaphragm ...................................................... 100
4.5.1 Removing the Pump Head and the Head Holder .......... 101
4.5.2 Cleaning and Replacement of the Plunger and the
Diaphragm ................................................... 103
4.6 Rinsing the Check Valve ................................... 107
4.7 Performing an Ultrasonic Cleaning of the
Check Valve .................................................... 110
4.8 Replacing the Check Valve ............................... 112
4.9 Inspecting the Line Filter ................................. 114
4.10 Replacing the Line Filter .................................. 115
4.11 Cleaning/Replacing the Suction Filter ................. 117
4.12 Replacing the Drain Valve ................................ 119
4.13 Replacing the Air Filter (Right Panel) ................ 120
4.14 Cleaning of the Leak Tray ................................ 122
4.15 Cleaning the Exterior ....................................... 122
4.15.1 Usual cleaning ............................................... 122
4.15.2 Decontamination cleaning .................................. 123
4.16 Cleaning of the Automatic Rinsing Kit .............. 123
Technical 125
5 Information
5.1 Specifications ...................................................
5.1.1 LC-40D X3 CL Specifications ............................... 125

5.1.2 LC-40D XR CL Specifications ............................... 126
5.1.3 High-Pressure Gradient System .............................. 127
5.1.4 Low-Pressure Gradient System .............................. 127

5.1.5 Other Specifications ......................................... 128
5.2 Maintenance Parts ........................................... 130
5.2.1 Consumable Parts ........................................... 131
5.2.2 Replacement Parts ........................................... 132
5.2.3 Maintenance Kit ............................................. 133
vi LC-40D X3 CL LC-40D XR CL
Contents
Index ......................................................... 134
LC-40D X3 CL LC-40D XR CL vii

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1 Overview
This instrument is a liquid delivery unit for liquid chromatography using a parallel double
plunger reciprocating-type pump developed to improve the precision and sensitivity of 1
analysis.
By pumping alternately from two pump heads arranged in parallel, it can perform
continuous pumping. The stepping motor's torque is transmitted to the cam, causing the
plungers to perform a piston motion.
1.1 Features
n Low Flow Pulsation and Pulsation Period Enable Precise Delivery
By reducing the discharge volume per plunger stroke to the microvolume level (10 µL),
and applying high-speed drive, the flow pulsation and pulsation period have been
reduced to levels significantly lower than those for other instruments.
n Choice of Gradient Modes and Control Options
LC-40D X3 CL/LC-40D XR CL can be used in both low- and high- pressure gradient modes.
The high-pressure gradient mode uses two pumps and is highly accurate with minimal
time lag. In low-pressure gradient mode, a single pump unit plus the gradient unit
provides a low cost option to combine up to four solvents.
There are two gradient modes available: fast LC mode or normal mode under control of
the system controller.
The fast LC mode for binary high-pressure gradient delivery supports ultra high speed
high separation analysis with minimized gradient control steps.
n Automatic Plunger Rinsing
An automatic rinsing kit is provided as standard for LC-40D X3 CL/LC-40D XR CL . It can

rinse the plunger in the back of the plunger seal. When high-salt-concentration buffer
solutions are used, in particular, it prevents premature seal failure due to crystallization,
extending the service life of plunger seals.
n High-pressure Flow is Available
LC-40D X3 CL enables ultra high pressure solvent delivery up to 130 MPa supporting ultra
fast high separation analysis in combination with Shim-pack XR-ODS III or other ultra fast
high separation columns. For LC-40D XR CL, high-pressure solvent delivery up to 70 MPa
is possible so high speed high separation analysis is supported in combination with
Shim-pack XR-ODSⅡcolumns.
LC-40D X3 CL LC-40D XR CL 1
1 Overview
1.2 Component Parts

This instrument consists of the standard parts listed below. Check the parts against this
list after unpacking.
No. Part Name Part No. Q'ty Remark

Main Unit
- LC-40D X3 CL/LC-40D XR - 1
CL
Safety Guideline
- 228-97195 1
(Booklet)*1
Instruction manual/
- 228-97193-41 1
System Guide (CD-ROM)
- Bottle, 250 mL 228-74372 1
1 Suction Filter Assy 228-45708-91 1 Including a tube
2 REMOTE CABLE ASSY 228-28253-91 1 Not used in CL model.
3 DRAIN TUBE SI(L1000) 228-25162-03 1
STOP JOINT ASSY,B,D 228-46054-91 1
4
SYRINGE TUBE ASSY,B,D 228-46055-91 1
SYRINGE,SS-20ESZ 228-66048 1
5 NEEDLE ASSY,FOR
228-18216-91 1
SYRINGE
6 CLAMP,M9120 046-05522-02 1
7 DRAIN ADAPTER 228-42204 1
8 STANDARD OUT DRAIN 228-42205 1
9 CTO OUT DRAIN 228-42206 1
10 STRAIGHT JOINT 228-28163 1
11 FITTING,L-TYPE 1253 035-61561-12 1
*1 It contains cautions for use regarding the instrument.
2 LC-40D X3 CL LC-40D XR CL
1.3 Optional Parts
1.3 Optional Parts

Optional units available for this instrument are listed below.
For information about other optional units listed below, contact your Shimadzu
1
representative.
n Common Optional Parts for LC-40D X3 CL/LC-40D XR CL
Option Part No. Features
Switches between up to 4 liquids of low-pressure

gradient or mobile phase. It also can be used for
auto rinsing of column flow lines.
Installed inside of the instrument.
Low pressure gradient
228-65016-58
kit
• Switches between 2 liquids (for example,

switches between mobile phase and rinse
solution) or two mobile phases.
• Installed inside of the instrument.
Reservoir switching
228-65017-58
valve
(w/ mixer
Mixer installation kit Gradient mixer (40 µL internal capacity)
recognition
(MR40 LPGE, to be exclusively for low-pressure gradient analysis.
device)
built in) Fixed inside of the instrument.
228-65020-41
(w/o mixer
Mixer MR40 LPGE Gradient mixer (40 µL internal capacity)
recognition
(fixed inside column exclusively for low-pressure gradient analysis.
device)
oven) It can be installed inside of the CTO-40C CL.
228-45210-41
(w/ mixer
Gradient mixer (300 µL internal capacity)
recognition
exclusively for low-pressure gradient analysis.
device)
It can be installed inside of the CTO-40C CL.
228-72653-42
Mixer MR300 LPGE This option can be used for solvents that are
(w/o mixer
hard to mix, such as TFA (trifluoroacetic acid), or
recognition
for reducing the baseline fluctuation of the
device)
photo diode array detector.
228-45210-42
Gradient Mixer for
High-Pressure Gradient "Gradient Mixer for High-Pressure Gradient Analysis" P.5
Analysis
1 Overview

The rinsing pump kit is intended for
continuously rinsing the back part of the plunger
RINSING PUMP XR 228-39625-42
seal with rinsing solution. The rinsing volume
can be selected.
Kit containing the necessary tools for
Tool Kit 228-57647-43
preparation, inspection, and maintenance.
Plumbing for the pump outlet. ID 0.3 mm
SUS Pipe (0.3´600) 228-53184-54
´600 mm, pre-bent
Plumbing for the pump outlet. ID 0.3 mm

SUS Pipe HP (0.3´1,000) 228-53184-96
´1000 mm
The maximum available pressure is 44MPa.

When using a non-polar organic solvent such as
Plunger Seal GFP 228-52711-94
hexane or a solvent of alkyl sulfonic acid with
TFA, use the standard plunger seal.
Suction filter with tube and fittings for the low
SUCTION FILTER ASSY 228-61905-49
pressure gradient kit or the degassing unit.
n Optional Parts for LC-40D X3 CL

A set of consumable parts and plumbing parts
MAINTENANCE for LC-40D X3 CL.
228-53265-45
KIT,LC-40D X3 "Maintenance Kit for LC-40D X3 CL (Parts
No. 228-53265-45)" P.133
n Optional Parts for LC-40D XR CL

A set of consumable parts and plumbing parts.
MAINTENANCE KIT,
228-45593-49 "Maintenance Kit for LC-40D XR CL (Parts
LC-40D XR
No. 228-45593-49)" P.133
1.3 Optional Parts
n Gradient Mixer for High-Pressure Gradient Analysis
The following gradient mixers for high-pressure gradient analysis are available for liquid
chromatographs.
1
Part Name Part No.
(w/ mixer recognition device) 228-72652-41
MIXER,MR 20 µL
(w/o mixer recognition device) 228-45209-41
MIXER,MR 40 µL
MIXER,MR 100 µL
MIXER,MR 180 µL Ⅱ
Mixers described above are packaged in a set
Part Name Part No.

Set of Two Mixers MR 40 µL and MR 180 µLⅡ 228-45292-41
Set of Two Mixers MR 100 µL and MR 180 µLⅡ 228-45292-42
Set of Three Mixers MR 40 µL, MR 100 µL and MR 180 µLⅡ 228-45292-43
Recommended Mixers
The table below lists the mixers with the smallest volume among mixers that can be used
in individual combinations of detectors and mobile phases.
Mobile Phase
Water (Including
Solvent Containing Solvent Containing Buffer Solution),
Trifluoroacetic Formic Acid, Organic Solvent,
Acid (TFA) Acetic Acid, etc. or Its Solvent
Mixture
Photo Diode Array
MR 180 µL MR 180 µL MR 180 µL
Detector
Detector
UV Detector MR 180 µL MR 100 µL MR 40 µL
LCMS, LCMSMS - MR 20 µL MR 20 µL
Using a mixer with a volume smaller than the recommendation shown above may cause
excessive fluctuation of the baseline.
When connecting multiple detectors, use a mixer with the largest volume among the
applicable mixers. For example, if using a Photo Diode Array and an LC/MS, the MR 180
µLⅡmixer is recommended.
A larger than recommended mixer can be used to improve mixing performance, but will
also increase the gradient delay volume.
1 Overview
n Automatic Rinsing Kit
The plunger can be rinsed with the automatic rinsing kit at the back part of the plunger
seal. LC-40D X3 CL/LC-40D XR CL has the automatic rinsing kit as standard. When using
buffer solution, be sure to attach the automatic rinsing kit.
See the table below for the necessity of the automatic rinsing kit and rinsing solution.
LC-40D X3 CL/LC-40D XR CL
Mobile Phase Recommended Rinsing
Necessity
Solution
Buffer Solution Necessary 10 % 2-propanol water
Normal-phase solvent such as hexane Not necessary -
Other Solutions Necessary 10 % 2-propanol water
Necessary: The rinsing kit is necessary.

Not necessary: The rinsing kit should not be used.
• For the application system, refer to the instruction manual of the application
system.
• If the rinse solution is dirty, replace with new solution. If getting significantly
dirty, replace with new solution once a day. Even if it does not appear dirty,
replace with new solution once a week.
If using distilled water as rinsing solution, replace it once a day.
2 Parts Identification and Function
2.1 Front
No. Name Description

Configure or operate the instrument with the operation keys.
1 Operation Panel Touching the center part on the operation panel displays the
operation keys.
2 Front Cover This is the cover for the flow line.
Switches ON/OFF the power.
Pressing the switch turns on the power and pressing it again
3 (Power button) turns off the power.
The switch is enabled only when the main power switch on
the back is on.
2.2 Behind Front Cover

1 Pump Head*1 The plunger inside it reciprocates to deliver solvent.
It has the rinsing flow path of the plunger inside. The
2 Head Holder*1
pump head is attached to this part.
3 Check Valve OUT*1 A check valve on the outlet side.
It is used to replace mobile phase solvent or remove
4 Drain Valve air from the flow path. It has an internal pressure
sensor.
A column protection filter for removing contaminants
5 Line Filter
from mobile phase solvent.
A connection port of the tube connected to the
6 Pump Outlet
autosampler/manual injector.
7 Check Valve IN*1 A check valve on the inlet side.
8 Pump Inlet A connection port of the tube from the suction filter.
It detects liquid leaks.

If the leak sensor detects a leak, delivery automatically
stops, causing an alarm and message on the display.
9 Leak Sensor
*1 This is common to the right and left pump heads.
2.3 Back
2.3 Back

1 [REMOTE] Connector A connector for connecting the system controller.
External Input/Output
2 Connect to external equipment.
Terminals
To input the pressure signal from the external
3 [DGU PRESS IN] Connector
degassing unit.
A connector for connecting rinsing pump or degassing
4 [DGU/SV] Connector
unit.
5 [PC] Connector A connector for service personnel. Normally unused.
6 Power Cord Connector Connect the power cord.
It turns ON/OFF the power to the instrument. Normally
7 Main Power Switch
keep it on.*1
*1 Normally use (power button) of the operation panel or system controller to turn ON/OFF the
power. If the system controller is connected and the main power switch is on, the user can use
(power button) of the system controller to turn ON/OFF the power from the front of the instrument.
If the instrument is not used for a long time, turn off the main power switch. Before turning off
the main power switch, be sure to turn OFF the power using the power button.
"2.5 Name and Functions of the Operation Panel" P.11
2.4 Right Side and Bottom

1 Mixer Mounting Holes Used to install mixer and column holder.
To prevent damage during transportation. LC-40D X3
CL has three screws, and LC-40D XR CL has two screws.
2 Shipping Screws (Red)
Remove before installation.
3 Leakage Drain Outlet Connect the provided drain tubing.
2.5 Name and Functions of the Operation Panel

This instrument is controlled through the keypad of the operation area. The display area
allows verification of the instrument status.
The operation area has two input modes: [Arrow key input mode] to enable screen
transfer, and [Numerical key input mode] to enable value input.
Pressing [Enter] switches between these input modes.
When turning off the main power switch on the back of the instrument after 2
changing the parameters via panel operation, be sure to turn OFF the power
with the power button at the front of the instrument, and turn off the main
power switch. Otherwise, some of the changed parameters may return to their
original values.

1 Display Area Displays various screens and settings.
2 Link LED Illuminates when controlled by the system controller.
• Green: analysis ready
• Red: error
• Blue: analysis in progress
3 Status LED
• Yellow: preparation for analysis in progress (The indicator
normally lights in yellow while no solvent is delivered.)
• Orange: sleep
Switches ON/OFF the power.

• To turn the power ON:
Press and hold the power button for at least 3 seconds.
• To turn the power OFF:
(Power Holding down the power button 3 seconds or more
4 displays the confirmation screen as shown below. Holding
Button)*1 it down again 1 second turns off the power.
Press [CE] to cancel the operation.
Delivery start/stop, gradient concentration setting,

5 Direct Key
timeprogram start and purge can be performed directly.
*1 If CBM-40 CL is used, pressing the power button of the pump turns off the power of the entire
system.
Note, however, that the following will apply in the case of a system with multiple pumps.
- When using CBM-40 CL: The system power can be turned off using the power button of the
pump with the lowest optical link number.
2.5.1 Display Area
In this section the display on the initial screen is described.
No. Name Function

In the constant flow delivery mode, the set flow rate (mL/min) is
displayed.
1 Flow/Press (Only for LC-40D XR CL) In the constant pressure delivery mode,
the set pressure is displayed in the pressure unit set with the
[PRESSURE UNIT] auxiliary function.
Displays reading measured by pressure sensor, in units set with
2 Pressure
the [PRESSURE UNIT] auxiliary function.
The maximum pressure is displayed in the pressure unit set with
3 P.MAX
The minimum pressure is displayed in the pressure unit set with
4 P.MIN
5 PUMP Highlighted during pump operation.
Highlighted in the low-pressure gradient mode or blinks in the
6 CONC
high-speed LC mode.
Highlighted during execution of a time program or in the
7 RUN
high-speed LC mode.
8 PURGE Highlighted during purging.
2.5.2 Operation Area
Use the keys on the front to operate or configure the instrument. Touch the key to make
the status LED brighter, accept input when you release the key.
No. Key Name Function

1 [PUMP] Pump Key Starts or stops the pump.
Concentration
2 [CONC] Sets liquid concentrations in gradient analysis.
Key
Starts or stops a time program. (Key operation is
3 [RUN] Run Key
ignored if a time program is not configured.)
Starts or stops purging. Purging stops automatically 3
minutes after it begins.
[PUMP] can also be used to stop purging. Purging time
can be set with the [PURGE TIME] auxiliary function.
4 [PURGE] Purge Key
Refer "3.8 Delivery in the Low-Pressure Gradient
Mode" P.76for the operation method on low
-pressure gradient mode.
This key is used for the following;

• To initialize the screen
5 [CE] Clear Key • To clear the values input up to that time while
entering the values.
• To clear error message and cancel alarms.
Use in the following cases.
• To delete input values.
6 [del] Delete Key
• To delete a line of the displayed time program while
creating it.
7 [Enter] Enter Key Confirms input values of the setting items
[↑][→]
8 Arrow Keys Moves the cursor or switches the screen
[↓][←]
9 [·] to [9] Numeric Keys Inputs values for the setting items.
No. Key Name Function

10 [ - ] Minus Key No use
Regarding the acceptable part of the arrow keys on the touch panel.
When operating the arrow keys, it
responds by touching the part including
the upper and lower range of each key
indication on the operation panel.
No. Description 2
1 Acceptable part of [↑] key input.
2 Acceptable part of [→] key input.
3 Acceptable part of [↓] key input.
4 Acceptable part of [←] key input.
3 Operation
3.1 Settings for the Basic Operation
Before operating this instrument, it is necessary to set the flow rate and the pressure
limit to protect flow line parts such as columns.
See the product specifications before use.
"5.1.1 LC-40D X3 CL Specifications" P.125
"5.1.2 LC-40D XR CL Specifications" P.126
3.1.1 Prior to Key Operation
Touching the central part of the operation panel displays the operation keys that the
user can operate.
3.1.2 Setting Flow Rate
The following is the basic procedure for setting a flow rate [FLOW].
1 Displays the initial screen (the screen

that is displayed when the power is
turned ON).
If the initial screen is not displayed, press
[CE] to display the initial screen.
2 Press [Enter].
The cursor blinks at the input position indicating that the flow rate [FLOW] can be input.
3 Input the flow rate with the numeric keypad and press [Enter].
The flow rate [FLOW] is set and the display returns to the initial screen.
To cancel the input value, press [CE].
3.1.3 Setting Compressibility
1 Press [→] twice on the initial screen.

The FUNCTION setting group is displayed.
3.1 Settings for the Basic Operation
2 Move the cursor to [PARAMETER], and

press [→].
The PARAMETER setting group is displayed.
3 Press [↓] several times until [COMP]

(compressibility correction) is displayed
in the screen and press [Enter].
The cursor blinks at the input position
indicating that the compressibility [COMP]
can be input.
4 Input the compressibility [COMP] for the solvent to be used (0.45 for water) with 3
the numeric keypad.
"Setting the compressibility of solvent《COMP》" P.32
3.1.4 Setting Maximum Pressure Limit
The maximum pressure limit is the pressure

in the flow line that may not be exceeded.
If pressure exceeds the maximum limit,

pumping stops automatically, and the error
message will be displayed with the alarm
sound.
n Setting Procedure
The following is an example to set the maximum pressure [P.MAX] to 15.0 MPa.
1 Press [→] three times on the initial screen.

2 Press [Enter].
indicating that [P.MAX] can be input.
3 Operation
3 Press [1], [5], [ . ], [0] and [Enter].

The maximum pressure is set to 15.0 MPa.
The allowable maximum pressure is 130 MPa for LC-40D X3 and 70 MPa for
LC-40D XR CL. When connecting the instrument with other equipment, set the
maximum pressure to the lowest allowable maximum pressure of the equipment.
"Setting the maximum pressure limit during pumping《P.MAX》" P.31
3.1.5 Setting Minimum Pressure Limit
The purpose of the minimum pressure limit

is to prevent a pressure drop which can
occur as a result of the following
situations:
• When the mobile phase runs out, air will
be pumped through the flow lines resulting in a pressure drop.
• When a leak occurs in the flow lines, a pressure drop may be observed.
If the pressure is lower than the minimum
pressure limit after one minute during
pumping, the flow stops automatically.
Then the error message will be displayed
with the alarm sound.
n Setting example
The following is an example to set the minimum pressure [P.MIN] to 2.0 MPa.
1 Press [→] three times on the initial screen.

2 Press [↓] once and on the [P.MIN]

screen, press [Enter].
indicating that [P.MIN] can be input.
3.2 Operation
3 Press [2], [ . ], [0] and [Enter].

The minimum pressure is set to 2.0 MPa.
If [P.MIN] is set to "0", solvent delivery will not stop and an alarm will not
sound even if the pressure has dropped. The minimum pressure can be set to
130 MPa max. for LC-40D X3 CL and 60 MPa max. for LC-40D XR CL.
"Setting the minimum pressure during delivery《P.MIN》" P.31
3.2 Operation 3
3.2.1 Preparation before Operation
1 Pour the mobile phase into the reservoir bottle.
2 Place a suction filter into the reservoir bottle.
3 Connect the tubing provided to the pump outlet.
4 Put the end of the drain tubing into the waste container.
5 Turn the drain valve knob

90°counterclockwise to open the drain
valve.
3 Operation
6 Press [PURGE], and then [ENTER].
During delivery, first press [pump]

and [ENTER] to stop the delivery,
then press [purge] and [ENTER].
IF the drain valve knob is turned more than 180°, any mobile phase that drains
out may contain air bubbles. This is normal.
3.2.2 Operation in Constant Flow Delivery Mode
There are two basic operating modes; one is in constant flow delivery mode and the
other is in constant pressure delivery mode.
1 Turn the drain valve knob clockwise as far as possible to close the drain valve.
2 Press [CE].
The initial screen is displayed.
3 Press [Enter] once and set the flow

rate to be delivered.
To set 1 mL/min, press [1] and [Enter].
4 Press [PUMP], and then [ENTER].

[PUMP] on the display is highlighted and
the pump starts operation.
5 Check that the pump outlet pressure is increasing on the pressure indication
display.
3.2 Operation
6 To stop operation, press [PUMP], and then [ENTER].

[PUMP] on the display stops being highlighted and the pump stops operation.
Close the drain valve when not draining through the drain path.
If the drain valve is left opened, a pressure difference may cause the mobile
phase solvent to flow out of the drain outlet.
3.2.3 Operation in Constant Pressure Delivery Mode
This function is available only for LC-40D XR CL.

3
1 Turn the drain valve knob clockwise as far as possible to close the drain valve.
2 Press [→] twice on the initial screen.

The FUNCTION setting group is displayed.
3 Press [↓] once, move the cursor to

[CONTROL] and press [→].
The CONTROL setting group is displayed.
4 Press [↓] twice and on the [MODE

CHANGE] screen, press [Enter].
The numeric keypad becomes active
allowing the user to input numbers.
5 Press [1] and [Enter].

The delivery mode changes from the
constant flow delivery mode to the
constant pressure delivery mode.
"Switching the delivery mode《MODE CHANGE》(Only for LC-40D XR CL)" P.38
6 After changing the mode, press [CE]

once.
The initial screen of the constant pressure
delivery mode is displayed.
3 Operation
7 Press [Enter].
[press] becomes ready to accept input.
8 Set the pressure with the numeric

keypad.
To set 2.0 MPa, press [2], [ . ], [0] and

[Enter].
9 Press [PUMP], and then [ENTER].

[PUMP] on the display is highlighted and the pump starts operation.
10 Observe that the pump outlet pressure rises and pressure display stabilizes at
about 2.0 MPa.
While monitoring the pressure, increase the flow rate until the pressure reaches the set
value. If increasing the flow rate does not increase the pressure, stop increasing the flow
rate at 1 mL/min.
11 To stop operation, press [PUMP], and then [ENTER].

[PUMP] on the display stops being highlighted and the pump stops operation.
3.3 Types of Screens
3.3 Types of Screens

Turning ON the power displays the initial screen.
By pressing the [↓] and [→] arrows on the initial screen, the screen can be switched
from the initial screen to one of three screens below:
Displays Screen Description

View the status of the instrument.
Monitor screen
"3.4 Monitoring Screens" P.24
Configure various parameter settings and perform operations.

Auxiliary functions screen
"3.5 Auxiliary Functions Screen (FUNCTION)" P.28
Supports the validation of the instrument with functions for

VP function screen viewing and checking instrument information. 3
"3.6 VP Functions Screen" P.47
Pressing [→] on the initial screen displays the screen to select the auxiliary functions
screen [FUNCTION] or VP functions screen [VP]. Move the cursor to the desired option
with [↓] or [↑] and press [→] to display the screen. Press [←] to return to the previous
screen. And Press [CE] to return to the initial screen.
3 Operation
3.4 Monitoring Screens

This section describes the monitoring
screens.
Press [↓] on the initial screen to show the
various types of information.
Name Description Reference

MONITOR To show the actual flow rate during delivery. The elapsed
P.24
FLOW/TIME time is shown while a time program runs.
To show the pump ID and the flow path of the flow path
MONITOR ID/SV P.24
switching valve.
To show the Auto-Diagnostics result when
Auto-Diagnostics is on. The delivery status of the pump is
shown when Auto-Diagnostics is off.
"Turning on/off Auto-Diagnostics《AUTO
DIAGNOSTICS》"
CONDITION P.68
• Auto-Diagnostics can cause a warning when a
delivery failure is detected.
• This screen is not shown in the constant pressure

delivery mode.
DEGAS PRESSURE To show the vacuum pressure of the degassing unit. P.26
To show the status of the connection between the
PC/WS*1 P.27
instrument and the PC/WS.
*1 Shown only when CBM-40 CL is connected.
n Monitoring Program Elapsed Time《MONITOR FLOW/TIME》
On the right side of the first line, the flow

rate actually delivered is displayed in real
time when the instrument is in the
constant pressure delivery mode or while
the flow rate is gradually increased with
the [FLOW SLOPE] function. The elapsed
time is displayed on the right side of the second line while a time program runs.
"Setting the time to reach the set flow rate after the start of delivery《FLOW SLOPE》"
n Displaying the pump ID and the port of the flow path switching valve connected to
the instrument during remote control《MONITOR ID/SV》
When the system controller is connected,

one of the pump connection addresses (A,
B, C, or D) of the system controller is
displayed on the lower left side of the
screen.
On the right side of the screen, the open ports of the flow path switching valve
connected to the instrument are displayed. In the previous figure, the connection address
of the system controller is set to A. When the low-pressure gradient valve is used, the
set ports open only during delivery. While delivery is stopped, the screen displays
"OFF".
n Displays of Condition《CONDITION》
The screen displays a delivery status

Auto-Diagnostics result during analysis
when the Auto-Diagnostics function is on.
This screen is not displayed in the constant
pressure delivery mode.
"Switching the delivery mode《MODE
CHANGE》(Only for LC-40D XR CL)"
P.38
3
Display Auto-Diagnostics Status

The delivery status is not being
judged. This is displayed while
Diag Off
delivery is stopped or when not
being analysed etc.
Judgment of the delivery status is

Diag Monitoring
in progress.
Diag NotDetected Delivery failure was not detected.
Diag Detected Delivery failure was detected.
Auto-Diagnostics issues a
warning if a pulse exceeds a
specific threshold.
Note that very slight pressure
variation may not be detected.
The table shows a guideline of the conditions under which the Auto-Diagnostics function
can correctly detect a delivery failure.
Item Specification
Constant flow pumping
Pumping Methods "Switching the delivery mode《MODE CHANGE》(Only for
LC-40D XR CL)" P.38
0.1 to 2 mL/min
Flow Rate * Pumps should satisfy the above flow rate range in the high
-pressure gradient mode.
3 Operation
Item Specification
LC-40D X3 CL: 5 to 130 MPa
Delivery Pressure
LC-40D XR CL: 5 to 70 MPa
Isocratic, two-solvent high-pressure gradient, two-solvent
Pumping Modes
low-pressure gradient
The Auto-Diagnostics function may not work in case of a significant pressure

decrease, such as when a large bubble enters the pump head. For this reason,
set the lower pressure limit P.MIN when using the function.
When Auto-Diagnostics is off, change in

the pump pressure is detected and a
delivery status is displayed.
Display Delivery Status

Press Stable Delivery is stable.
The pressure of the pump head that is on the left when viewed
Press Dec Left
from the front is decreasing during discharge.
The pressure of the pump head that is on the right when viewed
Press Dec Right
from the front is decreasing during discharge.
Press Increase The pressure is at least 10 % higher than that 5 minutes ago.
Press Decrease The pressure is at least 10 % lower than that 5 minutes ago.
[Press Increase] and [Press Decrease] are also displayed during normal operation,
such as when the pumps start and stop.
If [Press Dec Right] is displayed, it may indicate one of the following:
• Air remains inside the right pump head.
• There is a leak in the right pump head's seal.
• The right pump head's inlet check valve or the left pump head's outlet check
valve are not operating properly.
If [Press Dec Left] is displayed, it may indicate that conditions described above
have occurred for the left pump head.
"Turning on/off Auto-Diagnostics《AUTO DIAGNOSTICS》" P.68
n Monitoring Vacuum Pressure in Degassing Unit《DEGAS PRESSURE》
The screen displays the vacuum pressure of

the degassing unit connected to the
instrument.
• [(OK)] is displayed when the vacuum
pressure is at a normal value.
• [(NG)] is displayed when the vacuum pressure is at an abnormal value.
[Disconnected] is displayed when a

degassing unit is not connected.
n Displaying the status of connection with PC/WS《PC/WS》
The screen displays the status of

connection with a PC/WS.
[Connected] is displayed when the
instrument is connected with a PC/WS.
This screen is displayed only when the
CBM-40 CL is connected.
[Disconnected] is displayed when the instrument is not connected with a PC/WS.
3
3 Operation
3.5 Auxiliary Functions Screen (FUNCTION)

The auxiliary functions screen has six setting groups. Switch the groups with [↑] or [↓]
and press [→] to enter the screen of the desired setting group. Press [CE] to return to
the initial screen.
Setting Group
Description
Name
PARAMETER The group allows the user to set analysis parameters.
CONTROL The group enables specific operation.
The group allows the user to set parameters related to the system such
SYSTEM
as parameters of connection with external equipment.
The group allows the user to set parameters not related to the analysis
UTILITY
result such as display and buzzer parameters.
EDIT Time programs can be edited.
FILE Not used in CL model.
3.5.1 Auxiliary Functions (FUNCTION) List
n [PARAMETER] settings group
Name Description Remark Ref.

Initial value: 10
Set value:
Sets the maximum pressure limit
P.MAX (LC-40D X3 CL) 1.0 to P.31
for solvent delivery.
130.0 MPa
(LC-40D XR CL) 1.0 to 70.0 MPa
Initial value: 0
Sets the minimum pressure limit Set value:
P.MIN P.31
for solvent delivery. (LC-40D X3 CL) 0 to 130.0 MPa
(LC-40D XR CL) 0 to 60.0 MPa
Makes fine adjustment of
Initial value: 0.45
COMP compressibility correction of P.32
Set value: 0.00 to 3.00
solvent.
Sets the port of the internal Initial value: 1
INT.SV PORT low-pressure gradient kit or Set value: P.33
mobile phase switching valve. 1: A 2: B 3: C 4: D
EXT.SV PORT
Not used in CL model* Initial value: 1 -
CH1/CH2/CH3
EVENT1 Not used in CL model* Initial value: 0 -
EVENT2 Not used in CL model* Initial value: 0 -
Initial value: 0
Set value:
Sets the low-pressure gradient 0: Standard mode
LPGE CYCLE P.35
operation mode. 2: 2, 4, and 8-cycle mode
4: 4-cycle mode
8: 8-cycle mode

Initial value: 3
PURGE TIME Sets a purge execution time. P.35
Set value: 1 to 20 minutes
Initial value: 4.0
PURGE FLOW Sets a purge flow rate. Set value: P.35
0.1 to 10.0 mL/min
Sets maximum pressure limit at Initial value: 10.0
PURGE P.MAX P.36
purging. Set value: 1.0 to 10.0 MPa
Sets the delivery amount of the
RINSING Initial value: 0 (OFF)
optional rinsing pump when the P.36
OPERATE Set value: 0 to 4
pump is used.
Initial value: 0
Sets the time to reach the set
FLOW SLOPE Set value: P.37
flow rate during delivery.
0 to 30.00 minutes
* Do not change the factory default value. 3
n [CONTROL] settings group

Moves the plunger position. Initial value: 0
PLUNGER SET Replace the plunger or the Set value: P.37
plunger seal. 0: Stop 1: L 2: R 3: M
Performs zero adjustment of
ZERO ADJUST - P.37
pressure screen.
(Only for LC-40D XR CL) Initial value: 0
MODE Switches between the constant Set value:
P.38
CHANGE flow delivery mode and the 0: Constant flow
constant pressure delivery mode. 1: Constant pressure
n [SYSTEM] setting group

Initial value: 0
Selects independent operation or
LOCAL MODE Set value: P.38
control via system controller.
0: Remote 1: Local
Sets address of the instrument for Initial value: 4
LINK ADDRESS P.38
control via system controller. Set value: 1 to 12
CONTROLLER Selects the type of the connected Initial value: 1
P.39
LINK system controller. Set value: 0: Int 1: Ext
SELECT EVENT1 Not used in CL model.* Initial value: 0 -
SELECT EVENT2 Not used in CL model.* Initial value: 0 -
Initial value: 1
Set value:
PUMP CONFIG Input values according to the
1: Standard P.40
(SYS) system used.
4: Low-pressure gradient
mode
Reduces the flow rate without
SYSTEM stopping the pumping when the Initial value: 0
P.40
PROTECT pressure exceeds the P.MAX Set value: 0: Clear 1: Set
value.
3 Operation

EXT.SV TYPE Not used in CL model.* Initial value: 0 -
EXT.SV SERIAL Not used in CL model. Initial value: - -
EXT.SV
Not used in CL model.* Initial value: 0 -
CHANNEL
Initial value: 0
Sets the unit of the displayed Set value:
PRESSURE UNIT P.42
pressure. 0: MPa 1: kgf/cm2
2: bar 3: psi
INNER Initial value: 0
Input the tube volume. P.43
VOLUME Set value: 0 to 9999.9
* Do not change the factory default value.
n [UTILITY] setting group

KEY CLOSE Disables the key input. - P.44
Sets the brightness of display Initial value: 4
BRIGHTNESS P.44
screen. Set value: 1 to 4
Initial value: 0
BEEP MODE Sets the operation of buzzer. Set value: P.44
0: ON 1: Alarm 2: OFF
Initial value: 2
VOLUME Sets the buzzer volume. P.44
Set value: 1 to 3
The operation panel turns off after Initial value: 1
DISP OFF TIME P.44
the time set here elapses. Set value: 0 to 10 min
Specifies whether to display the Initial value: 0
DIRECT KEY
confirmation screen when the Set value: P.45
MODE
direct key is operated. 0: Confirm 1: Direct
n [EDIT] group
Use this group to create a time program. For details, see "3.7 Creating Time Program"
P.69.
n [FILE] operation group
This function is not used because events are not used in the CL model.
Name Description Remark

Initial value: 0
FILE NUMBER Selects a program file No.
Set value: 0 to 9
Name Description Remark

Initial value: 0
FILE COPY Copies a file.
Set value: 0 to 9
FILE DELETE Deletes a time program from a file. -
About files:
The instrument can store up to 10 files of flow rate and other parameter values, and
time programs in memory. File No. 0 to 9 are assigned to each file. The auxiliary
functions except [INT.SV PORT] and [EXT.SV PORT] are common functions.
Use [FILE NUMBER] of the file operation group to switch the files.
3.5.2 [PARAMETER] Setting Group
This setting group is relevant to solvent delivery parameters. 3
n Setting the maximum pressure limit during pumping《P.MAX》
When the delivery pressure exceeds the set

value, an error will occur and stop delivery.
Input the value with the numeric keypad
and press [Enter].
Setting Range
Setting Step
LC-40D X3 CL LC-40D XR CL
1.0 to 130.0 MPa 1.0 to 70.0 MPa 0.1 MPa
10 to 1326 kgf/cm 10 to 714 kgf/cm 1 kgf/cm
10 to 1300 bar 10 to 700 bar 1 bar
142 to 19000 psi 142 to 10157 psi 1 psi
1 kgf/cm2 = 0.098 MPa = 0.98 bar = 14.2 psi
n Setting the minimum pressure during delivery《P.MIN》
When the delivery pressure falls below the

set value for a specific time, an error will
occur and stop delivery.
Input the value with the numeric keypad
and press [Enter].
Setting Range
Setting Step
0 to 130.0 MPa 0 to 60.0 MPa 0.1 MPa
0 to 1326 kgf/cm2 0 to 612 kgf/cm2 1 kgf/cm2
0 to 1300 bar 0 to 600 bar 1 bar
0 to 19000 psi 0 to 8706 psi 1 psi
3 Operation
1 kgf/cm2 = 0.098 MPa = 0.98 bar = 14.2 psi
n Setting the compressibility of solvent《COMP》
Although the instrument corrects the

compressibility to suppress pulse increase
caused by the effect of the compressibility
of the solvent, setting a compressibility
suitable for the solvent used enables more
accurate correction. Mobile Phase compressibility (GPa)-1
Input the compressibility (GPa)-1 with the Water 0.45
numeric keypad and press [Enter]. The
Acetonitrile 1.20
initial value is 0.45.
Methanol 1.25
Ethanol 1.20
2-propanol 1.20
Hexane 1.60
Heptane 1.25
Cyclohexane 1.25
Ethyl Acetate 1.10
Chloroform 1.10
Benzene 1.00
Single solvent
Set the compressibility according to the table.
Mixture of water and organic solvent

If the concentration of the organic solvent is 50 % or less, "0.45" is recommended for
the compressibility of water. If the concentration is more than 50 %, make the value
closer to the compressibility of organic solvent according to the mixture ratio.
Example) When the ratio between water and acetonitrile is 50 : 50, set "0.45".
When the ratio between water and acetonitrile is 30 : 70, set "0.75".
In other cases or when rigorous adjustment is required
1 Connect a resistance tube (or column) that can create pressure similar to the
desired delivery pressure at 1.0 mL/min or less and wait until delivery becomes
stable.
2 While monitoring fluctuation on the pressure monitor of the instrument,

gradually increase the set value of [COMP] from 0.45 in 0.05 increments to find
the COMP value with the minimum fluctuation on the pressure monitor.
In the low-pressure gradient mode, the
setting screen will be as shown in the
figure.
Press [→] to enter the [COMP A] (mobile
phase A compressibility) setting screen.
Press [↓] or [↑] to set the compressibilitys
of the four solvents (A, B, C, and D).
Solvents are delivered at the
compressibility optimized according to the
set mixture ratio.
3
n Setting the port of the internal solenoid valve《INT.SV PORT》
Select the mobile phase port used for (Default screen)

delivery when the optional low-pressure
gradient kit is used as a mobile phase
switching valve or when the internal
reservoir switching valve is used. Input the
value (the set value in the table) of the (Screen for low-pressure gradient kit)
desired mobile phase port with the

numeric keypad and press [Enter].
Solenoid Valve Type Set value Mobile Phase Port

1 Solvent A
2 Solvent B
Low-Pressure Gradient Kit
3 Solvent C
4 Solvent D
1 Solvent A
Reservoir Switching Valve
2 Solvent B
The value cannot be input if the instrument has no internal solenoid valve.
3 Operation
n Setting the flow path of the mobile phase switching valve connected to the back of
the instrument《EXT.SV PORT CH1 / CH2 / CH3》
Not used in CL model.

Do not change the factory default value.
n Setting the EVENT1 output terminal《EVENT1》

Set "ON" (close) / "OFF" (open) of the

[EVENT1] output (relay contact) on the
back of the instrument.
Set
EVENT1 Output
Value
0 Relay 1 OFF
1 Relay 1 ON
n Setting the EVENT2 output terminal《EVENT2》

Set "ON" (close) / "OFF" (open) of the

[EVENT2] output (relay contact) on the
back of the instrument.
Set
EVENT2 Output
Value
0 Relay 2 OFF
1 Relay 2 ON
n Setting the low-pressure gradient mode《LPGE CYCLE》
Set the operation mode of low-pressure

gradient.
Set Value Low-Pressure Gradient Mode

The mode is automatically selected according to the flow rate.
0
2.0000 mL/min or less: 4-cycle mode
(Standard)
2.0001 mL/min or more: 8-cycle mode
2-cycle mode
Select this mode when using a low-capacity mixer with 300 µL or less
2 internal capacity.
In any other mode, concentration will be more uneven resulting in a 3
wavier baseline on the detector.
4-cycle mode
4 This mode is suitable for a mixer with approximately 0.5 mL internal
capacity.
8-cycle mode
8 This mode is suitable for a mixer with a 1.5 mL or higher internal
capacity.
n Setting the purging time《PURGE TIME》
Set the purging time.

The value can be set in the range from 1
minute to 20 minutes in one minute
increments.
n Setting the purging flow rate《PURGE FLOW》
Set the purging flow rate. This setting is

effective for both purging with the
[PURGE] operation in the instrument and
auto purging with the system controller.
The value can be set in the range from 0.1 mL/min to 10.0 mL/min in 0.1 mL/min
increments.
The flow rate set here is approximate for replacing the mobile phase in the
pump.
3 Operation
n Setting the maximum pressure during purging《PURGE P.MAX》
Set the maximum pressure during auto

purging.
For a purging operation started by the

[PURGE] key of the instrument, the
maximum pressure limit function works at
2.0 MPa and displays the error shown in
the figure as well as stopping delivery.
Setting Range Setting Step

1.0 to 10.0 MPa 0.1 MPa
10 to 102 kgf/cm2 1 kgf/cm2
10 to 100 bar 1 bar
142 to 1451 psi 1 psi
1 kgf/cm2 = 0.098 MPa = 0.98 bar = 14.2 psi
n Setting the delivery amount of the rinsing pump《RINSING OPERATE》
Set the delivery amount of the optional

rinsing pump.
During delivery or purging operation, if
the setting is "standard", the rinsing pump
starts with 6-second delivery and then
delivers rinsing solution for 2 seconds at intervals of 1 minute. The other settings double
or triple the 2-second delivery time.
The user can set the delivery amount to one of the four levels below.
Selecting "0" disables the rinsing pump.
Based on the delivery amount per hour in the following table, prepare necessary the
amount of the rinse solution for the analysis time.
• The delivery amount in the table is approximate for rinsing.

• Select "0" when not using the rinsing pump.
• The approximate delivery amount necessary for replacing the rinse solution in the rinsing
flow path is approximately 10 mL.
• To replace the rinse solution in the rinsing flow path, select "4" and operate the pump
approximately 3 minutes.
Set Value Delivery Amount per Hour (mL/h) Remark

0 0 The rinsing pump is disabled.
1 30 Half of standard
2 60 Standard
3 120 Twofold amount of standard
4 240 Fourfold amount of standard
n Setting the time to reach the set flow rate after the start of delivery《FLOW SLOPE》
Set the time to reach the set flow rate

after the start of delivery.
The value can be set in the range from
0 minutes to 30.00 minutes in 0.01 minute
increments.
3
Selecting "0" disables this function.
3.5.3 [CONTROL] Setting Group
n Setting the plunger stop position《PLUNGER SET》
Set the plunger stop position. This setting

is used for maintenance.
the plunger starts moving. [PUMP] on the
display stops being highlighted after the
plunger finishes moving.
Set Value Function

0 The plunger stops moving.
The plunger on the left when viewed from the front is retracted.
1
In this state, the left pump head is removed.
The plunger on the right when viewed from the front is retracted.
2
In this state, the right pump head is removed.
3 The plunger stops at the middle position.
n Zero adjustment of pressure sensor《ZERO ADJUST》
Set the reference value for the zero point of the pressure sensor.
1 Open the drain valve to remove

pressure completely.
2 Press [Enter].
3 Operation
n Switching the delivery mode《MODE CHANGE》(Only for LC-40D XR CL)
Switch between the constant flow delivery

mode and the constant pressure delivery
mode.
Stop the pump before switching the delivery mode. Switching the delivery mode
while the pump is operating is not possible.
3.5.4 [SYSTEM] Setting Group
This group is relevant to the system configuration such as connection with external
equipment.
n Selecting the local/remote mode《LOCAL MODE》
When the system controller is connected,

the user can select whether to operate the
instrument independently or with the
system controller.
Set Value Mode Function

0 Remote The instrument is operated with the system controller.
1 Local The instrument is operated independently (local mode).
n Setting the link address《LINK ADDRESS》
Set the address (channel No.) used to

connect with the system controller.
Address Function
1 to 12 A channel number for connecting with the system controller
n Setting the link destination of the system controller《CONTROLLER LINK》
Select the type of the connected system

controller.
Set Value Function

0 Not used in CL model.
The instrument is connected to an external system controller (CBM-40 CL) via
1
an optical cable connected to the [REMOTE] connector.
n Setting the function of the EVENT1 output terminal《SELECT EVENT1》 3

Set the mode of controlling external

equipment via the [EVENT1] output (relay
1).
Set Value Function

0 The relay contact is controlled with the [EVENT1] set value.
1 The relay contact 1 [EVENT1] is used as a start signal of a time program.
If the SELECT EVENT1 function is used, the corresponding EVENT parameter is

disabled.
n Setting the function of the EVENT2 output terminal《SELECT EVENT2》

3 Operation
Set the mode of controlling external

equipment via the [EVENT2] output (relay
2).
Set Value Function

0 The relay contact is controlled with the [EVENT2] set value.
1 The relay contact 2 [EVENT2] is used as an error output signal.
If the SELECT EVENT2 function is used, the corresponding EVENT parameter is

disabled.
n Setting the system parameter《PUMP CONFIG (SYS)》
Input values according to the status of the

system used.
Set Value Function

1 The instrument is controlled independently or with external equipment
4 The instrument is used as a low-pressure gradient system
The system functions normally only with the above values. Values other than the
above cannot be set.
Setting the set value to "4" (low-pressure

gradient system) highlights [CONC] on the
display allowing the user to set a
concentration.
n Setting the system protection《SYSTEM PROTECT》
When [P.MAX] is active, the flow rate is

reduced in increments of a half rate while
delivery is continued until the pressure
falls below [P.MAX].
Set Value Function

0 Do not use system protection.
1 Use system protection.
To clear a [P.MAX] error, press [CE]. The alarm stops and the flow rate returns to that
before the error.
n Selecting the type of external solenoid valve《EXT.SV TYPE》

Select the type of flow switching valve

(optional) connected to the [SV] connector
on the back of the instrument.
3
Set Value Function
0 Do not use FCV.
n Inputting the serial number of the external flow path switching valve《EXT.SV SERIAL》
Input the serial number of the flow path

switching valve connected to the SV
connector on the back of the instrument.
n Selecting the flow path of the solenoid valve unit《EXT.SV CHANNEL》
3 Operation
Set Value Port

0 The port does not pass through a flow path of FCV-11AL/FCV-11ALS
n Setting the unit of the displayed pressure《PRESSURE UNIT》
Set the unit of the displayed pressure.
Set
Unit of Displayed Value
Value
0 MPa
1 kgf/cm2
2 bar
3 psi
1 kgf/cm2 = 0.098 MPa = 0.98 bar = 14.2 psi
n Inputting the tube volume《INNER VOLUME》
Inputting a value in a low-pressure

gradient system enables the system
controller to calculate the system volume.
Refer to the table to input an appropriate
value according to the tubes used.
Input "0" in any mode other than the low-pressure gradient mode.
Tubing Between Solenoid Valve Tubing Between Pump Outlet

Set Value
and Pump Inlet and Mixer Inlet
Standard tube SUS Tubing (ID 0.3 x 600) 200 µL

(OUT tube, 310 mm) SUS Tubing (ID 0.3 x 300) 180 µL
Tube for buffer solution SUS Tubing (ID 0.3 x 600) 355 µL
(OUT tube, 400 mm)
3
SUS Tubing (ID 0.3 x 300) 335 µL
Use the Mixer installation kit
SUS Tubing (ID 0.3 x 185) 0 µL
(228-65020-41)
• The values above are intended when using the mixer recognition device. When the mixer
recognition device is not used, add the capacity depending on the mixer.
The column oven automatically recognise the capacity of the mixer when using the mixer
recognition device.
• The values above are intended for the recommended tubes. When using a tube not listed
above, increase or decrease the capacity depending on the tube.
Example
1) Use the standard tube + SUS Tubing (ID 0.3 x 600) + Mixer MR40 LPGE (without the
mixer recognition device)
→Set value: 240 (200 µL （from the table above） + 40 µL (mixer capacity))
2) Using the tube for buffer solution + SUS Tubing (ID 0.3 x 600) + Mixer MR40 LPGE
(with the mixer recognition device)
→Set value: 355（from the table above） (The capacity of the mixer will be
automatically recognised by the column oven)
3) Use the mixer instllation kit (without the mixer recognition device)
→Set value: 40 (0 µL （from the table above） + 40 µL (mixer capacity))
3 Operation
3.5.5 [UTILITY] Setting Group
This setting group is relevant to the display monitor.
n Disables the key entry《KEY CLOSE》
Press [Enter] to disable key input.

From this point, keypad input is disabled.
To release this function, press [Enter] while pressing [CE].
n Setting the brightness of display screen《BRIGHTNESS》
Sets the brightness of the display screen.

The value range is 1 to 4, and 4 is the
brightest.
n Setting the operation of buzzer《BEEP MODE》
Sets the buzzer sound.
Set Value Function

The key input sound, as well as the alarm sound to go off when an error
0
occurs, are activated. (Default setting)
1 Only the alarm sound that goes off when an error occurs is activated.
2 All the buzzer sounds are deactivated.
n Setting the operation of buzzer volume《VOLUME》
Set the buzzer sound level. A larger setting

value increases the sound level.
n Setting the time to turn off the operation panel automatically《DISP OFF TIME》
Sets the time elapsed before the operation

panel automatically turns to the sleep
mode, when no operations are performed
on the instrument.
The setting range is from 0 to 10 (min), in which the step can be set by minute.
If 0 (min) is input, the operation panel remains on.
Setting "0" minutes causes the display screen to be always on. If the display
screen is always on, some dots frequently lighting will deteriorate in brightness
in a shorter time.
n Setting the direct keys《DIRECT KEY MODE》
To prevent unintended operation, a

confirmation screen can be displayed when
a direct key ([PUMP], [RUN], or [PURGE]) is
pressed.
For example, setting this function to "0"

(Confirm) and then pressing [PUMP] 3
displays the confirmation screen shown in
the figure. Pressing [Enter] starts the
operation.
When the optional low-pressure gradient kit or reservoir switching valve is installed inside,
pressing [PURGE] when starting purging does not display the confirmation screen. The
confirmation screen, however, is displayed when purging is stopped.
3 Operation
3.5.6 [FILE] Operation Group
This function is not used because events are not used in the CL model.
n Specifying the file number《FILE NUMBER》
The instrument allows the user to create

and store a maximum of ten time
programs. The program files can be
selected with the parameter.
Select the file number from 0 to 9.
Input the file number with the numeric keypad and press [Enter].
n Specifying the file copy destination《FILE COPY》
The program content of the currently

selected file (indicated by the number of
[FILE NUMBER] above) is copied to the file
of a specified number.
Input a file number with the numeric
keypad and press [Enter].
n Deleting a file《FILE DELETE》
The time program of the currently selected

file is deleted.
Press [Enter].
3.6 VP Functions Screen

The VP functions screen has four setting groups. Switch the groups with [↑] or [↓] and
press [→] to enter the screen of the desired setting group. Press [CE] to return to the
initial screen.
Setting Group Name Description

PRODUCT INFO The group is relevant to information of the instrument.
MAINTENANCE The group is relevant to maintenance of the instrument.
The group allows the user to check whether the instrument is
VALIDATION
operating correctly.
CALIBRATION The group is for calibration of the instrument.
3
3.6.1 List of VP Functions
n Product information group [PRODUCT INFO]
Name Function Ref.

SERIAL NUMBER Displays the serial number of the instrument. P.49
S/W ID Displays the S/W version number. P.49
n Maintenance information group [MAINTENANCE]
Name Function Ref.

TOTAL OP TIME Displays the total operation time of the instrument. P.49
Displays or reset the total delivery amount of the
L SEAL USED currently used plunger seal and the delivery amount that P.49
requires replacement (for the left seal).
Displays or reset the total delivery amount of the
R SEAL USED currently used plunger seal and the delivery amount that P.49
requires replacement (for the right seal).
Displays the remaining amount of mobile phase and set
MOBILE PHASE*1 P.50
an amount.
Specifies a threshold to cause an alarm of low mobile
ALARM LEVEL P.50
phase level.
Input the part number of the replaced part when
PART REPLACEMENT P.51
replacing the part.
MAINTENANCE LOG Displays the maintenance log. P.51
OPERATION LOG Displays the log of password change, parameter reset, etc. P.51
ERROR LOG Displays the error log. P.52
Displays or reset the operation time of the degassing unit
DGU OP TIME P.52
connected to the instrument.
*1 Normally one solvent can be set for the mobile phase level monitoring function. Only in the
low-pressure gradient mode, four solvents (A to D) can be set.
3 Operation
n Validation support information group [VALIDATION]
Name Function Ref.

DATE Displays or set the date. P.53
TIME Displays or set the time. P.53
MEMORY CHECK Checks ROM/RAM. P.54
LEAK SENSOR CHECK Checks operation of the leak sensor. P.54
PULSE CHECK Checks the pulse. P.55
FLOW CHECK Checks the flow rate. P.56
Checks that the maximum and minimum pressure
PRESS LIMIT CHECK P.60
functions work correctly.
n Calibration support information group [CALIBRATION]
Name Function Ref.

Input PASSWORD*1 Input a password. P.61
OPERATION MODE Not used in CL model.*2 -
DATE FORMAT Changes the displayed order of year, month and day. P.62
INITIALIZE PARAM Initializes parameters. P.62
PARAMETER LOCK To prohibit change of parameters. P.62
PARTS MGMT TOOL Sets the usage of the parts management tool. P.63
PASSWORD SETTING Changes the password. P.63
Displays or set the parameters of the connected system
CBM PARAMETER P.64
controller.
LEAK THOLD Sets the operation level of the leak sensor. P.66
FLOW COMP FACTOR Not used in CL model.*2 -
PRESS COMP FACTOR Not used in CL model.*2 -
PLUNGER SEAL Sets the time to replace the plunger seal. P.68
P.MAX OVERRIDE Sets override of the maximum pressure. P.68
SV SELECT Not used in CL model.*2 -
AUTO DIAGNOSTICS Turns on/off Auto-Diagnostics. P.68
* 1 In the calibration support group, an invalid password prevents the user from going further than
[OPERATION MODE] even if the user presses [Enter].
* 2 Do not change the factory default value.
3.6.2 Product Information Group (PRODUCT INFO)
The group is relevant to information of the instrument.
n Showing the serial number《SERIAL NUMBER》
Displays the serial number of the

instrument.
n Displaying the S/W version number《S/W ID》
The screen displays the S/W name (model

name) and version.
3
3.6.3 Maintenance Information Group (MAINTENANCE)
The group is relevant to maintenance information of the instrument.
n Showing the total operation time《TOTAL OP TIME》
Displays the total cumulative operating

time of the instrument.
n Displaying the delivery amount of the left plunger seal《L SEAL USED》
The screen displays the total delivery

amount of the currently used plunger seal
and the delivery amount that requires
replacement (unit: L).
If the parts management tool (PARTS MGMT TOOL) is not used, press [del] after replacing
the seal. The total delivery amount is reset to "0" and the reset date is recorded in the
maintenance log.
• The plunger seal on the left when viewed from the front is shown as "L".
• When PARTS MGMT TOOL is active, the delivery amount cannot be reset on the operation
panel. Reset it from PARTS MGMT TOOL. For the operation procedure, refer to the PARTS
MGMT TOOL instruction manual.
n Displaying the delivery amount of the right plunger seal《R SEAL USED》
The screen displays the total delivery

amount of the currently used plunger seal
and the delivery amount that requires
replacement (unit: L).
3 Operation
If the parts management tool (PARTS MGMT TOOL) is not used, press [del] after replacing
the seal. The total delivery amount is reset to "0" and the reset date is recorded in the
maintenance log.
• The plunger seal on the right when viewed from the front is shown as "R".
• When PARTS MGMT TOOL is active, the delivery amount cannot be reset on the operation
panel. Reset it from PARTS MGMT TOOL. For the operation procedure, refer to the PARTS
n Displaying and setting the remaining amount of mobile phase《MOBILE PHASE》
The screen displays the remaining amount

and set amount of the current mobile
phase.
When replacing the mobile phase, input
the set amount of the mobile phase in the
unit of mL and press [Enter].
The remaining amount is automatically
calculated when solvent is delivered.
Normally only one solvent is supported. In the low-pressure gradient mode, four solvents
(A to D) are supported.
A1, A2, B1, B2 etc. are shown if the
low-pressure gradient kit and external
mobile phase switching valve are used
together.
n Setting a threshold to cause an alarm of low mobile phase level《ALARM LEVEL》
The warning screen is displayed when the

mobile phase level has fallen to the set
percentage. Note that delivery continues.
Input the set value with the numeric
keypad and press [Enter]. Setting the value
to 0 % disables the low mobile phase level
alarm.
n Inputting a replacement part number《PART REPLACEMENT》
When the parts management tool (PARTS

MGMT TOOL) is not used, input the part
number when replacing a general part.
The input part number is stored in the
maintenance log.
When PARTS MGMT TOOL is active,

the delivery amount cannot be reset
on the operation panel. Reset it from
PARTS MGMT TOOL.
For the operation procedure, refer to
the PARTS MGMT TOOL instruction
manual.
3
"Displaying the maintenance log《MAINTENANCE LOG》" P.51
n Displaying the maintenance log《MAINTENANCE LOG》
This displays twenty records of previously

replaced parts with the dates of their
replacement. After press [→], press [↓]
several times to display the replaced parts
in order.
The figure shows an example that
"part number 228-48249-96 was replaced
on April 1, 2019".
The table shows the messages displayed in the second row of a maintenance log.
Message Description Ref.

L SEAL REPLACED The left plunger seal was replaced. P.49
R SEAL REPLACED The right plunger seal was replaced. P.49
P/N: XXX-XXXXX-XX The displayed part number was replaced. P.51
The setting value (******) was entered in DGU OP
DGU OP TIME: ****** P.52
TIME.
n Displaying the operation log《OPERATION LOG》
This displays ten records of previous

modifications such as password change and
parameter reset with the dates of the
modifications. After press [→], press [↓]
several times to display the operation log
records in order.
"the password was changed on April 1,
2019".
3 Operation
The table shows the messages displayed in the second row of a maintenance log.
Message Description Ref.

INITIALIZE PARAM The parameters were initialized. P.62
PARAM LOCK ON The parameter lock function was turned ON.
PARAM LOCK OFF The parameter lock function was turned OFF.
P.62
When the parameter lock function was ON, a user
PARAM LOGIN entered the password and moved on to the next
screen.
CHANGE PASSWORD The password was changed. P.63
USE PARTS MGMT The PARTS MGMT TOOL was set to "Use".
P.63
NOT USE PARTS MGMT The PARTS MGMT TOOL was set to "NotUse".
n Displaying the error log《ERROR LOG》
This displays ten records of previous errors

with the dates of the errors.
After press [→], press [↓] several times to
display the error log records in order.
"a maximum pressure error occurred on
April 1, 2019".
For the messages displayed in [ERROR
LOG], refer to the System Guide.
System Guide "5.4 Error Log List"
n Displaying the operation time of the degassing unit《DGU OP TIME》
The screen displays the operation time of

the degassing unit connected to the
instrument.
When the operation time is input, count
of the operation time of the degassing
unit starts from the input time. The input set value is stored in the maintenance log.
If [999999 h] is set, the display shows "******" and the operation time will not be
counted.
• To activate this function, supply power from the instrument to the degassing
unit and make sure that the pressure signal cable is connected correctly. If
more than one pump is used, be sure to connect the power cable and pressure
signal cable to the same pump. For how to connect, refer to the System Guide.
• The shutdown function of CBM-40 CL or LC-Workstation can stop the degassing
unit. For details, refer to the instruction manual of the system controller or
LC-Workstation.
• Approximate time to replace the vacuum pump of the degassing unit is 3 years
or 8000 hours of operation time. Contact your Shimadzu representative if you
need to replace the vacuum pump.
3.6.4 Validation Support Information Group (VALIDATION)
The group allows the user to check whether the equipment is operating correctly.
n Entering date《DATE》
This allows the user to view or input the

date in this screen. The value is reset to
the default "00-00-00" if the power switch
on the back is turned off. The value is
transmitted when the system controller is
connected for control.
To delete the input value, press [del].
The figure is an example of April 1, 2019. 3
n Entering time《TIME》
This allows the user to view or input the

time in this screen. The value returns to
the initial value "00:00:00" when the
power is turned off. The value is
transmitted when the system controller is
connected for control.
To delete the input value, press [del].
The figure is an example of 3:01:01 p.m.
3 Operation
n Checking the memory《MEMORY CHECK》
Runs the memory check on ROM and RAM.

Pressing [Enter] starts the memory check.
After the check, the result is displayed.
n Checking the leak sensor《LEAK SENSOR CHECK》
Performs the operation test for the leak sensor.
1 Use a syringe filled with water to wet

the sensor unit at the bottom of the
leak sensor.
2 Wait about 10 seconds and then press

[Enter].
If the sensor detects a leakage, [Good] will
be shown.
If not, [No Good] will be shown.
3 Press [CE].
The result display will be cleared.
If the result is [No Good], adjust the detection level with [LEAK THOLD] function in the
Calibration Support Group.
"Setting the operation level of leak sensor《LEAK THOLD》" P.66
After wetting and testing the leak sensor, wipe away the water on the tray
completely. When wiping away the water, do not apply pressure to the leak
sensor, the sensitivity decreases if the sensor unit touches the wall.
"4.14 Cleaning of the Leak Tray" P.122
n Checking the pulse《PULSE CHECK》
When checking operation, connect a resistance tube to the pump outlet to create 5 MPa
to 10 MPa pressure at 1 mL/min during delivery.
1 Press [Enter].
2 Set the acceptance criterion (MPa) of

pressure variation (pulse).
Input the set value with the numeric
keypad and press [Enter]. Start delivery at 3
1 mL/min.
The value range is 0.05 to 1.00 MPa.
• When setting 0.20 MPa as the acceptance criterion, input a value as shown in the figure.
Standard of the acceptance criterion (MPa) : 0.20 MPa or less (When connecting the
resistance tube 4 m x 0.1 mm I.D and delivery of water at 1 mL/min.)
The measurement starts after 1 minute

during pumping. During measurement, the
set flow rate (1 mL/min), measured
pressure, pressure variation, and remaining
time are displayed.
After measurement, the panel displays the

measured pressure variation with pass or
fail, and then the results and the
measurement date and time are stored in
the memory of the instrument.
Before measurement, make sure that the compressibility correction parameter of

the solvent is correctly set.
3 Operation
n Checking the flow rate《FLOW CHECK》
to 10 MPa pressure during delivery.
According to your measurement method, measure the flow rate by following the
instructions below.
Set Value Measurement Method

The flow rate is measured based on the time that was required to deliver
0
the specified volume. (volumetric method)
The flow rate is measured based on the weight of water delivered in the
1
specified time. (gravimetric method)
2 A flow meter is used for measurement
Before measurement, make sure that the compressibility correction parameter of

the solvent is correctly set.
To use the volumetric method
1 Set the flow rate and start delivery.
2 After confirming the delivery stabilizes, measure the time required for the
solvent to accumulate to 5 mL in a measuring flask.
3 In the [FLOW CHECK] screen, press

[Enter].
4 In the measurement method selection

screen, input "0".
5 Input the measured flow rate.

The value can be set between 0.1 mL/min
and 3.0 mL/min.
6 Set the acceptance criterion of the

flow rate accuracy in "ML".
The value can be set between 0.001 mL
and 1.000 mL.
For example, if the set flow rate is 1 mL/min and the criterion is ±2 %, input 0.02 mL.
7 Input the measurement time (seconds).
The flow rate accuracy (%) and pass/fail 3

result are displayed.
To use the gravimetric method
1 Measure the weight (g) before delivery.
3 After confirming the delivery stabilizes, accumulate water in a measuring flask

for a specific time.
4 Measure the weight (g) before delivery.

[Enter].

screen, input "1".

and 3.0 mL/min.
3 Operation

flow rate accuracy in mL.
and 1.000 mL.
9 Input the weight (g) measured before

delivery.
10 Input the measurement time (seconds).
11 Input the weight (g) measured after

delivery.
The flow rate accuracy (%) and pass/fail

To use a flow meter
2 After confirming the delivery stabilizes, measure the flow rate.

[Enter].

screen, input "2".

and 3.0 mL/min.

flow rate accuracy in mL.
and 1.000 mL.
3
7 Input the flow rate measured with a
flow meter.
The flow rate accuracy (%) and pass/fail

3 Operation
n Checking operation of the maximum and minimum pressure functions 《PRESS LIMIT
CHECK》
to 10 MPa pressure at 1 mL/min during delivery.
1 Press [Enter].
Start delivery at 1 mL/min. The pressure is

monitored for 1 minute and the maximum
pressure (P.MAX) and minimum pressure
(P.MIN) are automatically set.
Then the flow rate is automatically set to

1.5 mL/min and 0.5 mL/min, and whether
the P.MAX and P.MIN functions work
correctly is checked.
The result is displayed after checking is

completed.
3.6.5 Calibration Support Information Group (CALIBRATION)
The group is for calibration of the instrument.
The instrument is tuned before shipment. Do not modify values unnecessarily.
n Inputting the password《Input PASSWORD》
The password must be input by the system

administrator.
Input the five-digit numbers by using the
numeric keypad, and press [Enter].
3
Be sure to input the five numbers. The default password is [00000].
When the password matches, the [OPERATION MODE] screen below appears on the screen.
If the password does not match, the user cannot go further.
n Setting the operation mode《OPERATION MODE》
This function is not used in the CL model.

The factory default value : 0
3 Operation
n Changing the displayed order of year, month and day《DATE FORMAT》
Change the displayed order of year, month

and day.
Input the value by using the numeric
keypad, and press [Enter].
Set value Display

0 Year, month, and day (YMD)
1 Day, month, and year (DMY)
2 Month, day, and year (MDY)
n Initializing the parameters《INITIALIZE PARAM》
Initialize the parameters and the time

programs.
Press [Enter] to return to the default
values of the parameters and to delete the
time programs.
After the parameter initialization
completes, the instrument is automatically
restarted.
n Setting the parameter lock《PARAMETER LOCK》
This prevents users other than the

administrator from changing parameters
except the flow rate by prohibiting screen
transition to the auxiliary functions screen
and VP functions screen.
If [1] (On) is set, a password input screen is displayed when the user enters the setting
screen. If the user modifies the setting or inputs the password to enter the setting screen,
the operation is recorded in the operation log.
"Displaying the operation log《OPERATION LOG》" P.51
n Setting the usage of the parts management tool《PARTS MGMT TOOL》
Sets the usage of the parts management

tool.
For details on the parts management tool
(PARTS MGMT TOOL), refer to the PARTS
Set value Description

0 Do not use PARTS MGMT TOOL.
1 Use PARTS MGMT TOOL.
When the setting is changed, the change is recorded in the operation log.
"Displaying the operation log《OPERATION LOG》" P.51
3
n Changing the password《PASSWORD SETTING》
Enables to change the password.
1 Press [Enter].
The input screen is displayed.
2 Input the five-digit number by using

the numeric keypad, and press [Enter].
3 To confirm, input the same password

that was input in step 2 again.
When the input is completed and the
password is changed, the message
"PASSWORD CHANGED" is displayed. Then,
the password change is recorded in the
operation log.
If the password input is not correct, the
message "PASSWORD WRONG" is
displayed. At this stage, the password has
not been changed yet.
4 Press [Enter] to return to the title screen.
3 Operation
n Showing/setting CBM parameter《CBM PARAMETER》
This allows the user to view or set the

parameters of CBM-40 CL that controls the
instrument.
Press [→] to enter the CBM parameter setting screen.

Press [↓] or [↑] several times to select the desired item.
Pressing [←] at an item in the screen returns the display to the screen as shown in the
figure.
If CBM-40 CL is not connected (it is not set the link address), pressing [→] does not bring
the user to the CBM parameter setting screen.
"Setting the link address《LINK ADDRESS》" P.38

"Setting the link destination of the system controller《CONTROLLER LINK》" P.39
List of CBM Parameters
Display Description
SERIAL NUMBER Displays the serial No. of CBM.
S/W ID Displays the program version No. of CBM.
INTERFACE Sets the transmitting protocol to data processing unit.
ETHERNET SPEED Sets the transmitting speed of Ethernet.*1
USE GATEWAY Sets usage of default gateway or DHCP server.*1
IP ADDRESS Sets IP address of CBM.*1
SUBNET MASK Sets subnet mask.*1
DEFAULT GATEWAY Sets default gateway.*1, *2
Select the communication distribution when connecting to an

TRS MODE
LC workstation or a Chromatopac.
*1 Available only to show, when not allowed to change on CBM-40 CL.

*2 Not available when [USE GATEWAY] is set to "Do not use Default Gateway".
Each parameter is activated after the system controller is restarted.
Refer to CBM-40 CL instruction manual for details of each parameter.
Showing the serial number《SERIAL NUMBER》

Displays the serial number of the system
controller which controls the instrument.
Showing the S/W version number《S/W ID》

Displays the S/W name (the same name as
the model name) and version of CBM
which controls the instrument.
Setting the transmitting protocol to data processing unit《INTERFACE》

Sets the transmitting protocol between the
data processing unit and the system
controller which controls the instrument.
Enter the value using the numeric keypad
and press [Enter].
Set Value Transmitting Protocol

0 To connect with optical cable.
1 To connect with serial transmission. (RS-232C)
2 To connect with Ethernet.
Setting the transmitting speed of Ethernet《ETHERNET SPEED》

Sets transmitting speed of the system 3
controller Ethernet which controls the
instrument.
and press [Enter].
Set Value Transmitting Speed

0 Auto Detect
1 10 Mbps, Half Duplex
2 10 Mbps, Full Duplex
3 100 Mbps, Half Duplex
4 100 Mbps, Full Duplex
Setting the usage of default gateway or DHCP server《USE GATEWAY》

Sets usage of default gateway or DHCP
server of the system controller which
controls the instrument.
and press [Enter].
Set Value Description

0 Do not use Default Gateway.
1 Use Default Gateway.
2 Automatic acquisition of IP address from DHCP server.
Setting IP address《IP ADDRESS》

Sets IP address of the controller which
and press [Enter].
3 Operation
Setting the subnet mask《SUBNET MASK》

Sets subnet mask of the controller which
and press [Enter].
Setting the default gateway《DEFAULT GATEWAY》

Sets the default gateway of the controller
which controls the instrument.
and press [Enter].
Setting the transmission mode《TRS MODE》

Select the communication distribution
when the controller is connected to an LC
workstation.
and press [Enter].

0 Connects in manual setting at the system controller.
2 Can not be used.
3 Connects to LabSolutions.
Do not set values other than the set value above. Otherwise the instrument will
not work properly.
n Setting the operation level of leak sensor《LEAK THOLD》
Enables to set the threshold value for the

leak sensor.
When an error occurs in the leak sensor
check, set the value again.
Calibration of the leak sensor must be performed by the system administrator.

The leak sensor detects liquid drops when the mobile phase leaks from the
plunger seal or tubes. When the sensor output value exceeds the predetermined
"threshold," the sensor issues an error and stops pumping.
1 Dip the sensor unit at the bottom of the leak sensor into water.
Upon detection of water, the leak sensor value (Actual Level) in the screen increases.
2 Read the maximum value of the leak sensor value (Actual Level).
Example) The maximum value here is "160".
3 Wipe off the water near the leak sensor.

The leak sensor value (Actual Value) will decrease.
When wiping away the water, do not apply pressure to the sensor unit of the
leak sensor. The sensitivity decreases if the sensor unit touches the instrument
wall surface.
4 Calculate the threshold of the leak sensor based on the value read in step 2.
Formula to calculate the leak sensor threshold:
Threshold = (Max. value-100)×0.7+100
Example) The value obtained here is "142".
3
5 Press [Enter], input the value obtained
in step 4, and press [Enter].
The new "threshold" is stored.
n Setting the flow rate correction parameter (ALPHA)《FLOW COMP FACTOR》

Recalibration operation is executed by service personnel.
This allows the service personnel to set the

flow rate correction parameter.
n Setting the pressure sensor sensitivity correction factor《PRESS COMP FACTOR》

This allows service personnel to set the

pressure sensor sensitivity correction factor.
3 Operation
n Setting the time to replace the plunger seal《PLUNGER SEAL》
This allows the user to set the time to

replace the plunger seal (unit: L).
"Displaying the delivery amount of the left plunger seal《L SEAL USED》" P.49
"Displaying the delivery amount of the right plunger seal《R SEAL USED》" P.49
n Selecting how to set the maximum pressure《P.MAX OVERRIDE》 (LC-40D XR CL only)
This allows the user to set the maximum

pressure with keys on the pump even if
[OPERATION MODE] is set to "1" and the
instrument is controlled with the system
controller.
If [OPERATION MODE] is set to "0", the set value will be "0".
"Setting the operation mode《OPERATION MODE》" P.61
Set Value Setting Method

0 To set the value with the system controller.
1 To set the value on the pump.
n Selecting the flow path switching valve《SV SELECT》
This allows the user to select the valve to

use when [OPERATION MODE] is set to "1"
(compatible mode) and the instrument is
controlled with the system controller.
n Turning on/off Auto-Diagnostics《AUTO DIAGNOSTICS》
This allows the user to turn on/off

Auto-Diagnostics.

0 Auto-Diagnostics is off.
3.7 Creating Time Program

1 Auto-Diagnostics is on.
"Displays of Condition《CONDITION》" P.25
• The function of the time program is not available when controlled by the
system controller. Configure from the workstation. See " system guide 2.2
Editing Methods"
• Refer to the following procedure when using this function in local mode.
The instrument can run a time program with a flow rate and other parameters. A created 3
time program is saved in a file. Check for file numbers in use before creating a time
program.
See "Specifying the file number《FILE NUMBER》" P.46 of the auxiliary function to view
or specify file numbers.
3.7.1 Time Program Command List
The commands for the time program are listed below.
Com-
Description Setting Range Remark Ref.
mand
Sets flow rate (Available
0 to Minimum unit:
FLOW only in constant flow P.20
10.0000 mL/min 0.0001 mL/min
solvent delivery mode)
Sets delivery pressure.
Minimum units:
(Available only in 10 to 60.0 MPa
0.1 MPa
constant pressure solvent 10 to 612 kgf/cm2
PRESS 1 kgf/cm2 P.21
delivery mode. This can 10 to 600 bar
1 bar
be set only for 142 to 8706 psi
1 psi
LC-40D XR CL)
Specifies concentration of
mobile phase B Minimum unit: *2
BCONC 0 to 100 %
Only active when [PUMP 0.1 %
CONFIG(SYS)] = 4*1
mobile phase C Minimum unit:
CCONC 0 to100 %*2
CONFIG(SYS)] = 4*1
mobile phase D Minimum unit:
DCONC 0 to100 %*2
CONFIG(SYS)] = 4*1
3 Operation
Com-
Description Setting Range Remark Ref.
mand
Opens or closes the
solenoid valve of the
INT.SV low-pressure gradient unit 1, 2, 3, 4 - P.32
or internal reservoir
switching valve (optional)
Opens or closes the
0, 1, 2, 3, 12, 13,
EXT.SV solenoid valve of Not used in CL model. -
23, 123
FCV-11A/FCV-11ALS
Sets ON/OFF of the event
output (relay contact) on
EVENT 0, 1, 2, 12 Not used in CL model. -
the back of the
instrument
0 to 255
LOOP Repeats program * 0 means 256 - P.75
times.
STOP Stops program N/A - P.75

Switches to other
GOTO 0 to 9 Not used in CL model. -
programs (up to 10 files)
*1 "Setting the system parameter《PUMP CONFIG (SYS)》" P.40

*2 The total of the settings for [BCONC], [CCONC], [DCONC] may not exceed 100 %.
That is: [BCONC] + [CCONC] + [DCONC] ≤ 100.
The concentration of mobile phase A will be the value subtracted from the total value of the other
concentrations from 100 (%).,
For example: Concentration A = 100 − ([BCONC] + [CCONC] + [DCONC])
"3.7.4 Creating a Low-Pressure Gradient Program" P.73
3.7.2 Time Program Setting Screen
To create a time program, access the edit screen as described below.
1 Press [CE].
The initial screen is displayed.
2 Press [→] twice.

The auxiliary functions screen [FUNCTION]
is displayed.
3 Press [↓] several times until [EDIT] is displayed in the screen, move the cursor
to [EDIT], and press [→].
The time program editing screen is
displayed. The figure shows an example of
a time program that has ten steps already
created and 310 steps left available.
Display Description
USED The number of created steps in the selected file
LEFT The number of steps left available
4 Press [Enter] again to edit steps of the

time program. 3
Displays Description
TIME Time (minute) that elapsed from the start of the step
FUNC Command name
VALUE Set value
3.7.3 Creating a Time Program
The table below shows an example of a

time program that changes the flow rate
as shown in the figure (initial flow rate:
1 mL/min).
FUNC Command VALUE(mL/min)

Step TIME (minute) Setting
Option Input a Command Value
1 15.00 FLOW 2.0000
2 20.00 FLOW 5.0000
3 30.00 STOP -
3 Operation
1 Displays the time program setting

screen.
"3.7.2 Time Program Setting Screen"
P.70
2 Press [Enter].
The time (minute) setting screen is
displayed.
To set step 1
3 Press [1], [5], and [Enter].
4 Press [Enter].
The [FLOW] command is selected, which
enables the user to input the flow rate.
5 Press [2] and [Enter].

The flow rate is set to 2 mL/min and
editing step 1 is completed.
To set step 2
6 Press [2], [0], and [Enter] to set the

time.
7 Press [Enter].
The [FLOW] command is selected, which enables the user to input the flow rate.
8 Press [5] and [Enter] to set the flow

rate.
To set step 3
9 Press [3], [0], and [Enter].
10 Press [↓] several times until [STOP] is

displayed in the command selection
screen.
11 Press [Enter].
12 Press [CE] to save the time program.

Then the initial screen is displayed.
3
When inputting multiple steps, the user does not need to input the data in order of time
because they are automatically sorted.
3.7.4 Creating a Low-Pressure Gradient Program
Setting the concentrations of solvents B, C, and D at individual time points in a time

program can change the concentrations of solvents A, B, C, and D with time.
The concentration of solvent A in this case will be as below.
Solvent A (%) = 100 (%) - Solvent B (BCONC) (%) - Solvent C (CCONC) (%) - Solvent D (DCONC)
(%)
Note that the solvent B (BCONC), solvent C (CCONC), and solvent D (DCONC) parameters
can be set in the range from 0 % to 100 % with the minimum unit of 0.1 %.
n Example of time program setting
1 Displays the time program setting

screen.
"3.7 Creating Time Program" P.69
2 Press [Enter] and input the time with the numeric keypad.
3 Operation
3 Press [↓] several times until [BCONC]

is displayed in the setting screen.
Press [↓] several times in the same manner to display [CCONC] and [DCONC].
4 Move the cursor to [BCONC] and press

[Enter].
The [BCONC] command is selected, which enables the user to set the solvent B
concentration.
5 Input the concentration with the numeric keypad and press [Enter].
3.7.5 Deleting a Step
To delete a step, display it and press [del].

See the following example of deleting the first step of the program created in "3.7.3
Creating a Time Program" P.71.
1 In the same manner as creating the

program, display the desired step.
To display the second or subsequent

lines, press [↓] several times until the
desired step is displayed.
2 Press [del].
The first step of the program is deleted
and the second step is displayed.
3.7.6 Starting and Stopping a Time Program
n Starting a Time Program
To start a time program after setting it,

perform the following.
Press [RUN] to start the program.
[RUN] on the display is highlighted.
n Stopping a Time Program
There are two ways to stop the program.

• Forcibly stopping a program being executed
Press [RUN] to stop the program.
[RUN] on the display stops being highlighted and the program stops.
• Stopping a program with the [STOP] command
"Setting a [STOP] command for a time program《STOP》" P.75
3.7.7 Commands Used for Time Programs Only
This section explains about the commands that can be set for time programs only.
3
n Setting a loop count for a time program《LOOP》
A [LOOP] command can repeat a program

the specified number of times.
Settings shown in the table repeat steps 1 and 2 three times at intervals of 30 minutes.
TIME VALUE
Step FUNC
(Minute) (mL/min)
1 15.00 FLOW 2.0000
2 20.00 FLOW 5.0000
3 30.00 LOOP 3
• After [LOOP] is repeated the specified number of times, the program

automatically stops.
• The [LOOP] command can be set to a maximum of 255. Note that if it is set
to "0", LOOP is executed 256 times.
n Setting a [STOP] command for a time program《STOP》
This allows the user to set the time to stop

a time program. Input the time in the last
step of a time program and press [↓]
several times until [STOP] is displayed.
Press [Enter] to set a [STOP] command.
When the time program is executed continuously, do not set a [STOP] command
at the end of the program.
3 Operation
3.8 Delivery in the Low-Pressure Gradient Mode
3.8.1 Set Value
1 Input "4" in the [PUMP CONFIG(SYS)]

of [SYSTEM] setting group.
"Setting the system parameter《PUMP CONFIG (SYS)》" P.40
2 Set the [P.MAX] and [P.MIN] of [PARAMETER] setting group.

"Setting the maximum pressure limit during pumping《P.MAX》" P.31
"Setting the minimum pressure during delivery《P.MIN》" P.31
3.8.2 Preparation for Delivery
1 Press [Enter] once on the initial screen.
2 Input a flow rate as the [FLOW] set

value with the numeric keypad.
3 Set solvent concentrations.
1 Press [CONC].
The concentration setting screen
is displayed.
2 Input the solvent B concentration

(%) and press [Enter].
3 Input the solvent C and D concentrations (%).

The concentration of solvent A in this case will be as below.
Solvent A concentration (%) = 100 (%) - Solvent B concentration (%) - Solvent

C concentration (%) - Solvent D concentration (%)
For example, when setting the solvent A

to D concentrations to 25%, input values
as shown in the figure.
3.8 Delivery in the Low-Pressure Gradient Mode
4 Set the compressibility.
1 Displays [COMP] of [PARAMETER]

setting group.
2 Press [→].
The compressibility setting screen
is displayed.
Press [↓] several times to display
the [COMP B], [COMP C], and
[COMP D] setting screens.
3 Set the compressibilitys of solvents A to D.

3
5 Purge flow paths.
1 Open the drain valve by turning it 90 degrees counterclockwise.
2 Press [PURGE].
The flow path selection screen is
displayed.
3 Select the flow path to purge with the numeric keypad and press [Enter].
[PUMP] on the display is highlighted and purging starts.

Solvent is purged at the concentration set on the concentration
0
setting screen.
1 Solvent A is purged.
2 Solvent B is purged.
3 Solvent C is purged.
4 Solvent D is purged.
6 Close the drain valve and start delivery.
3 Operation
3.9 Connection with the System Controller or Workstation
3.9.1 Setting the Instrument
To control the instrument from CBM-40 CL set the parameters as follows:
Setting
Set Value Remark
Parameter
"Selecting the local/remote mode《LOCAL MODE》"
LOCAL MODE 0: Remote
P.38
LINK ADDRESS Link address "Setting the link address《LINK ADDRESS》" P.38
CONTROLLER 0: Int "Setting the link destination of the system controller

LINK 1: Ext 《CONTROLLER LINK》" P.39
(LC-40D X3 CL)
OPERATION 0: 40D X3 CL Not used in CL model.

MODE (LC-40D XR CL) Do not change the factory default value.
0: 40D XR CL
3.9.2 Basic Parameters
A max. of 4 pump units can be connected to CBM-40 CL. Workstation can set flow rate
(pressure), maximum pressure limit, minimum pressure limit, solenoid valves, pumping
ON/OFF, time program, etc., and can control binary and ternary high-pressure gradient
analysis depending on the system configuration. Also, by combining the SIL-40 CL series
autosampler, the auto purging function is available. For details, refer to the system guide.
3.10 Connection to External Input/Output Terminals
In the CL model, do not connect another external device with the black-white
event cable.
Wiring is done by service personnel during installation.
4 Maintenance
4.1 Periodic Inspection and Maintenance
It is necessary to perform periodic inspections of this instrument to ensure its safe use.
It is possible to have these periodic inspections performed by Shimadzu service personnel
on a contractual basis.
For information regarding the maintenance inspection contract, contact your Shimadzu
representative.
Turn off the main power switch on the back of the instrument and unplug
the power cable prior to maintenance when instructed.
Fire, electric shock or malfunction may occur.
4
For parts replacement, use parts and tools listed in "1.2 Component Parts" P.2
and "5.2 Maintenance Parts" P.130.
If any other parts or tools are used, part damage, injury, and malfunction may
occur.
Never remove the cover.

This may cause injury or a malfunction of the device.
Contact your Shimadzu representative if the cover must be removed.
4.1.1 Prior to Inspection and Maintenance
• Loosen the male nuts (A and C) on the

column inlet and outlet with an 8-mm
wrench and then remove the column (B)
from the flow path. Attach the coupling
(D) to the male nut (A and C), and
tighten it with an 8 mm wrench.
• Replace the mobile phase in the flow
lines with at least 20mL of water.
When normal phase solvent was used,
replace the internal channel with
2-propanol and then with water.
• Wipe away any dirt from the front panel
and the main cover.
• Remove the right panel cover and filter
holder before starting the work. Release
4 Maintenance
the tubes bound at the front right from the tube holder to prevent them from
interfering with the work.
"4.2 Removing the Right Panel Cover and the Filter Holder" P.84
n Standard Reagents for Maintenance
Solvent Description Inspection/Maintenance Item

"4.4 Replacing the Plunger
Seal" P.92
"4.5 Cleaning/ Replacing the
Plunger and Diaphragm"
P.100
"4.6 Rinsing the Check Valve"
P.107
"4.7 Performing an Ultrasonic
2-propanol Used for cleaning parts. Cleaning of the Check Valve"
P.110
"Gradient Mixer for
High-Pressure Gradient
Analysis"
"4.12 Replacing the Drain
Valve" P.119
"4.16 Cleaning of the
Automatic Rinsing Kit" P.123
n Hardware Maintenance Supplies
Note that in addition to the following tools and parts are also required. The tools and
parts required for the maintenance of each part are listed at the beginning of each
section.
Item Description Inspection/Maintenance Item

Protective glasses Glasses and gloves before
ALL Inspection/Maintenance
Gloves the maintenance.

Seal" P.92
Used for wiping and
Wiping paper "4.5 Cleaning/ Replacing the
cleaning parts.
Plunger and Diaphragm"
P.100
Beaker Used for cleaning parts.
Seal" P.92
Seal" P.92
"4.7 Performing an
Ultrasonic Cleaning of the
Check Valve" P.110
Ultrasonic bath Used for cleaning parts. "Gradient Mixer for
High-Pressure Gradient
Analysis"
"4.16 Cleaning of the
Automatic Rinsing Kit"
P.123
Item Description Inspection/Maintenance Item

Used for gripping the
Tweezers plunger seal and backup
Seal" P.92
ring.
4 Maintenance
4.1.2 List of Periodic Inspection and Maintenance
The maintenance and replacement periods listed in this table are presented only
as guidelines. These are not guarantee periods.
These will vary depending on usage conditions.
Inspection/Maintenance Replacement
Remark Ref.
Item Periods
• Sealing efficiency decreases when seals
are worn.
• As a guideline, seals should be
replaced after delivery of the
respective volumes listed below. (VP
fuction, [L(R)SEAL USED], shows the
total delivered volume.)
(LC-40D X3 CL)
Pumping Pressure Total Delivery

80 MPa
30 L
(816 kgf/cm2)
Replacement of Plunger Repeating high-pressure (above 100

1 year P.92
Seal MPa) and low-pressure (below 10 MPa)
pumping could shorten the life of seals.
(LC-40D XR CL)
Pumping Pressure Total Delivery

30 MPa
60 L
(306 kgf/cm2)
60 MPa
30 L
(612 kgf/cm2)
Repeating high-pressure (above 60 MPa)

and low-pressure (below 10 MPa)
pumping could shorten the life of seals.
Replace the plunger seal and the
Replacement of Plunger 1 year diaphragm when the plunger is
replaced. P.100
Replacement of
1 year -
Diaphragm
Replacement of Outlet
1 year - P.112
Check Valve
Replacement of Inlet
1 year - P.112
Check Valve
Replace when particulates in mobile P.114
Replacement of Line Filter 2 years
phase clog the filter. P.115
Replacement of Suction Replace when particulates in mobile
2 years P.117
Filter phase clog the filter.
Replacement of Drain Replace when there is a fluid leak from
3 years P.119
Valve the drain valve.
Inspection/Maintenance Replacement
Remark Ref.
Item Periods
Replacement of Air Filter
1 year - P.120
(for Right Panel)
Replacement of Air Filter
1 year Contact your Shimadzu representative. -
(for FAN)
Pump ASSY lubrication 3 years Contact your Shimadzu representative. -
Replacement of Fuse 3 years Contact your Shimadzu representative. -
Cleaning of the
1 year - P.123
Automatic Rinsing Kit
Pumping mobile phase mixtures
containing acids such as trifluoroacetic
acid or formic acid can cause leakage
due to internal damage in the pump
Replacement of Pump head. In such cases, replace the pump
(2 years) -
Head head every two years, as a general
guideline.
(Pump heads do not need to be
replaced if only mobile phases without
acid are pumped.)
Inspection of Leak Sensor 1 year - P.54
4
n Optional Parts
Replacement
Inspection/Maintenance Item Remark Ref.
Periods
Replacing the Air Filter of Contact your Shimadzu
1 year -
Low Pressure Gradient Kit representative.
4.1.3 Check after Inspection and Maintenance
• After inspection and maintenance, check any leakage during pumping.

• Return the right panel cover, filter holder and tubes after completing the work.
When the parts management tool (PARTS MGMT TOOL) is not used, input the
part number when replacing a part not registered in the PARTS MGMT TOOL.
"Inputting a replacement part number《PART REPLACEMENT》" P.51
4 Maintenance
4.2 Removing the Right Panel Cover and the Filter Holder
1 Open the right panel.
2 Remove the right panel cover.

While lightly pressing the cylinder-shaped
projection (a) inside the right panel cover,
slide the cover to the front.
3 While pressing the knob at the top of

the filter holder (A), remove it toward
the front.
4.3 Removing/Installing the Pump Heads

This section will explain how to remove and re-attach the right-side pump head.
Removing and re-attaching the left-side pump head is also the same procedure.
Necessary tools
Part Name Quantity Part No.

Wrench (8 mm and 10 mm) 2 086-03002
Allen wrench (M5) 1 086-03805
Syringe 1 046-00038-01
Syringe needle 1 228-18216-91
n Prior to the maintenance Perform the preparatory work.
"4.1.1 Prior to Inspection and Maintenance" P.79
n Removing the pump head

4
1 Perform either of the following to prevent the mobile phase solvent from
flowing out of the suction tube or pump head when removing the tubes around
the pump.
• Place the reservoir bottle at a position lower than the pump inlet.
• Empty the reservoir bottle and suction tube of the mobile phase solvent, loosen the
bushing of the suction filter, and remove it from the pump inlet.
2 Press [CE] to display the initial screen.
3 Press [→] twice.
4 Press [↓] once, move the cursor to

[CONTROL], and press [→].
[PLUNGER SET] menu is displayed.
4 Maintenance
5 Press [Enter].
The cursor blinks at the input position.
6 Press [2] and [Enter]. (To replace the

left plunger seal, press [1] and [Enter].)
after a while stops being highlighted. The
right plunger is now at the backmost
position.
In the conditions, remove the right pump head.
Before attaching the right pump head, perform steps 2 to 6 as well.
7 Follow the instructions below to remove the tube (SUS pipe R) connecting the
check valve OUT and the pressure sensor.
(LC-40D X3 CL)
1 While holding the check valve

OUT (A) with a 10-mm wrench,
loosen the check valve OUT side
male nut (B) of the SUS pipe R
with an 8-mm wrench.
2 Loosen the pressure sensor side
male nut (C) of the SUS pipe R
3 Hold the check valve OUT side

male nut ("a" in the figure) of
the SUS pipe R and then slightly
lift and pull out the SUS pipe R.
4 Pull the SUS pipe R to the right

to remove it from the pressure
sensor.
Though the orientation is

opposite to the right one
(SUS pipe R), remove the
left tube (SUS pipe L) in
the same procedure.
(LC-40D XR CL)

loosen the check valve OUT side
male nut (B) of the SUS pipe R
2 Loosen the pressure sensor side
male nut (C) of the SUS pipe R 4
3 Hold the check valve OUT side

male nut ("a" in the figure) of
the SUS pipe R and then slightly
lift the SUS pipe R.
4 Maintenance
4 Slightly lean backwards the SUS

pipe R to pull it out upward.

(SUS pipe R), remove the
the same procedure.
8 Loosen the male nut PEEK (A) of the

inlet tube by hand and remove it from
the check valve IN (B).
9 If the seal rinsing kit is attached,

remove the cap (B) of the seal rinsing
kit from the rinse solution bottle (A).
10 Attach the syringe needle (C) to the

end of the outlet FEP tubing (E)
(upward U-shape), and use the syringe
(D) to suction out the rinse fluid from
the rinse flow line.
11 Place the removed cap (B) and rinse solution bottle on the reservoir tray on the
top of the instrument.
12 Gradually loosen the 2 hexagonal

socket screws using an Allen wrench
(M5) by alternating between the left
and right screw, and remove the pump
head.
Gently pull the pump head

horizontally along the plunger.
The plunger may be damaged
if the pump head is forcefully
removed.
n Installing the pump head
1 Set the plunger position by following steps 2 to 6 in "Removing the pump head"
P.85.
4
2 Hold the pump head with the mark (a)

coming at the bottom right, tighten
the 2 hexagonal socket screws (B) with
an Allen wrench (M5) by tightening
the right and left screws alternately,
and attach the pump head (A).
Gently inset the pump head

horizontally along the plunger.
The plunger may be damaged
if the pump head is forcefully
inserted.
Tighten the left and right hexagonal socket screws 90 degrees at a time, and
then firmly tighten using the long side of the Allen wrench.
4 Maintenance
3 Tighten the male nut PEEK (A) of the

inlet tube to the inlet check valve (B)
by hand.
4 Follow the instructions below to attach the tube (SUS pipe R) connecting the
check valve OUT and the pressure sensor.
1 Insert the end of the SUS pipe R

into the hole of the pressure
sensor.
2 Hold the check valve side end of

the SUS pipe R ("a" in the figure)
and then insert the male nut into
the check valve OUT (A).

tighten the SUS pipe R male nut
on the check valve OUT side with
an 8-mm wrench (B).
4 Tighten the pressure sensor side

male nut (C) of the SUS pipe R

(SUS pipe R), attach the
the same procedure.
5 Attach the original rinsing kit cap (B)

to the rinse solution bottle (A), and
then remove the outlet FEP tubing (E)
(upward U-shape) from the rinse 4
solution bottle.
6 Attach the syringe needle (C) to the

end of the FEP tubing (E) (upward
U-shape), and use the syringe (D) to
suction out the rinse solution from the
rinse flow line.
7 Return the rinsing kit to the reservoir tray.
4 Maintenance
4.4 Replacing the Plunger Seal

A plunger seal and backup ring are attached inside the left and right pump heads. The
following section will explain the method for replacing the plunger seal and backup ring
on the right side (when viewed from the front).
Replacing the plunger seal and backup ring on the left side are also the same procedure.
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
Allen wrench (M5) 1 086-03805
Seal Installer/Remover 1 228-62458-41
Necessary parts
Part Name Part Type Part No.

Plunger Seal (UHP)
Consumable part 228-52711-93
(supplied with backup ring)
n Prior to the maintenance Perform the preparatory work.
4.4.1 Replacing the Plunger Seal and Backup Ring
1 Remove the pump head, SUS pipe, and inlet tube by following the instructions
in "Removing the pump head" P.85.
2 Perform the following steps to replace the plunger seal and backup ring.
The plunger seal and the backup ring are installed inside the pump head. Use the
provided seal installer/remover to remove them.
Do NOT drop the seal installer/remover when using it.

Seal installer/remover may be damaged.
1 Wipe off the threaded tip on the

metal part (a) of the seal
installer/remover (A) with a
wiping paper soaked in
2-propanol.
2 With the metal part (a) of the

4
seal installer/remover (A) facing
downward, insert the seal
installer/remover (A) vertically
into the backup ring (B).
3 Turn the seal installer/remover (A)

180 degrees to 360 degrees
clockwise.
4 Maintenance
4 Pull out the seal installer/remover

(A) upward.
The backup ring (B) will come off
from the plunger seal hole (b) of
the pump head (C).
If the backup ring is

still stuck, return to
step 3 and turn the
seal installer/remover
180 degrees more,
then retry this step.
5 Grip the backup ring (B) with two

fingers and turn the seal
installer/remover
counterclockwise, then remove
the backup ring from the seal
installer/remover.
metal part of the seal
installer/remover with a wiping paper soaked in 2-propanol.
7 With the metal part of the seal

installer/remover facing
downward, place the seal
installer/remover vertically above
the plunger seal and push it
slightly into the seal.
8 Turn the seal installer/remover (A)

clockwise until it comes into
contact with the pump head (C).
9 Pull out the seal installer/remover

(A) upward.
The plunger seal (D) will come off
from the plunger seal hole (b) of
the pump head (C).
10 Place the pump head into a clean beaker containing 2-propanol, and clean it for
10 minutes using an ultrasonic bath.
11 Use a piece of wiping paper

soaked in 2-propanol to wipe the
plunger attachment portion (b)
inside the pump head (C).
4
• If seal material adheres to the plunger attachment portion of the

pump head, the airtightness cannot be maintained.
• If any scratches are found on the plunger attachment portion of
the pump head, replace the pump head.
12 Grip the plunger seal (D) with

two fingers and turn the seal
installer/remover
counterclockwise, then remove
the plunger seal from the seal
installer/remover.
metal part of the seal
installer/remover with a wiping paper soaked in 2-propanol.
14 Remove the cap (c) from the seal

installer/remover (A).
15 Wipe off the white protrusion (d)
and the surrounding area of the
seal installer/remover (A) with a
wiping paper soaked in
2-propanol.
4 Maintenance
16 Put some drops of 2-propanol in

the plunger seal hole (b) of the
pump head (C) while paying
attention to spillage of
2-propanol.
Before putting in 2-propanol, screw the check

valves (E) into the pump head (C) so that
2-propanol does not leak from the holes for the
check valve.
17 Soak a new plunger seal and a new backup ring in 2-propanol in a clean beaker.
When gripping the plunger seal with

tweezers, do NOT touch the internal
hole surface. Also, to prevent damage,
do NOT grip the plunger seal and the
backup ring tightly with tweezers.
18 Take the new plunger seal (D) out

of the beaker and put it on the
white protrusion (d) of the seal
installer/remover (A).
Be sure that the plunger seal is moistened with 2-propanol when putting it
on the seal installer/remover.
19 Insert the seal installer/remover

(A) vertically all the way into the
plunger seal hole (b) of the pump
head (B) and slowly pull out the
seal installer/remover upward.
The plunger seal will be installed
in the pump head.
20 Take the new backup ring (B) out

of the beaker and, with the
smaller diameter side facing the
seal installer/remover (A), put the
backup ring on the white
protrusion (d).
Be sure to attach the backup ring with

the correct orientation.
When the backup ring (B) is attached
correctly to the pump head, the larger
diameter side comes into contact with
the plunger seal (D).
Be sure that the backup ring is moistened with 2-propanol when putting it
on the seal installer/remover.
21 Insert the seal installer/remover

vertically all the way into the
plunger seal and slowly pull out
the seal installer/remover upward.
The new backup ring (B) will be
installed into the pump head (C).
4 Maintenance
22 Put the cap (c) on the white

protrusion (d) of the seal
installer/remover.
3 Attach the pump head, SUS pipe, and inlet tube by following the instructions in
"Installing the pump head" P.89.
4.4.2 Resetting the Delivery Amount
When PARTS MGMT TOOL is active, the delivery amount cannot be reset on the
operation panel. Reset it from PARTS MGMT TOOL.
1 Display the [MAINTENANCE]

information group of the VP function.
"3.3 Types of Screens" P.23
2 Press [↓] several times until [R SEAL USED] is displayed.
3 Press [del].
The delivery amount of the plunger seal
before replacement is reset and the reset
date is recorded in the maintenance log.
"Displaying the delivery amount of the right plunger seal《R SEAL USED》" P.49 4
To replace the left plunger seal, display [L SEAL USED] and press [del].
4.4.3 Check after Replacement
After replacing the plunger seal, check the following:

• Is the pumping (pressure) stable?
• Is fluid leaking from the gap between the pump head and the head holder?
• Is fluid leaking from the rinse flow line?
If the above occurs even after replacing the plunger seal, it is possible that there
are scratches on the surface of the plunger. If there are scratches on the plunger,
replace it with a new plunger.
"4.5 Cleaning/ Replacing the Plunger and Diaphragm" P.100
4 Maintenance
4.5 Cleaning/ Replacing the Plunger and Diaphragm

A plunger and a diaphragm are attached to the left and right pump body. The following
section will explain the method for cleaning/ Inspection (replacing) the plunger and the
diaphragm on the right side (when viewed from the front). Replacing the plunger and
a diaphragmthe on the left side are also the same procedure.
When replacing the plunger, be sure to also replace the plunger seal and the diaphragm.
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
Allen wrench M4 1 086-03804
Allen wrench M5 1 086-03805
Box driver 1 228-28767-91
Diaphragm jig 1 228-54852
Plunger press plate 1 228-70388
Necessary parts
Part Name Part Type Part No. Remark

Supplied with a
Plunger Holder Assy Consumable part 228-52069-44
diaphragm
n Prior to the inspection and the maintenance Perform the preparatory work.

4.5.1 Removing the Pump Head and the Head Holder
1 Remove the pump head, SUS pipe, and

inlet tube by following the instructions
in "Removing the pump head" P.85.
2 Loosen the head holder top and

bottom fittings (C and D), and remove
the rinse tube.
4
3 Gradually loosen the 2 hexagonal
socket screws (B) of the head holder
(A) using an Allen wrench (M4) by
alternating between the left and right
screw.
4 Remove the head holder.
Gently pull the head holder horizontally along the plunger.

If the head holder is not removed carefully, the plunger may become damaged.

4 Maintenance
n In the case the head holder is difficult to remove
Insert into the head holder M4 bolt hole

(a) the 2 hexagonal socket screws (B) that
were removed, and tighten the left and
right sides gradually using an Allen wrench
(M4) (D).
The head holder (A) can be loosened by

pushing the pump body (C) using the 2
hexagonal socket screws (B).

4.5.2 Cleaning and Replacement of the Plunger and the Diaphragm
When replacing the plunger, be sure to replace the plunger seal and the
diaphragms.
1 Perform the following steps to clean or replace the plunger and diaphragm.
1 Wipe plunger with a sheet of

wiping pape soaked with
2-propanol to remove any portion
of the seal or dirt attached to the
plunger (A).
If small pieces of the

plunger seal material
adheres to the
surface, the
airtightness cannot
be maintained.
4
2 Check that there are no visible scratches on the plunger (A) or the diaphragm (B).
• If scratches are found through visual inspection, replace with a new plunger.
Proceed to the next procedure 3.
• If no scratches are found through visual inspection, proceed to step 2.
Do NOT allow foreign particles or crystals into the head holder.

Foreign particles from the mobile phase solution or crystals from the buffer
solution getting into the head holder may cause scratches on the surface of
the plunger.

4 Maintenance
3 Insert the box driver (B) to the

plunger holder (A), then rotate
counter-clockwise to remove the
plunger holder with the
diaphragm.
Gently insert the box driver to

the plunger holder.
The plunger may become
damaged if the box driver is
forcefully inserted.
4 Attach a new diaphragm (B) to

the new plunger holder (A) using
the diaphragm jig (C).
Confirm the
direction of the
diaphragm when
attaching.
5 After applying the grease (black:

approximately 5 mm square size)
provided with the plunger holder
ASSY to the rear end surface of
the plunger (a), insert the plunger
holder (A) to which the
diaphragm was attached in
Procedure 4 into the pump body.
• Be careful not to get any grease on the diaphragm or the body.

• Attach the diaphragm so that it will not become detached from the
plunger holder. If the diaphragm becomes detached, wipe away any
grease and perform from Procedure 4 again.

6 Tighten the plunger holder (A)

using the box driver (B).
7 Pinch the end of the plunger with

your two fingers, move it up,
down, right, left, clockwise, and
counterclockwise five times each
so that the grease applied to the
rear end of the plunger holder
can spread evenly. Then wipe off
the plunger surface with wiper
paper soaked in 2-propanol to clean it.
2 In the [PLUNGER SET] screen of the control setting group, press [2] and [Enter].
(To replace the left plunger, press [1] and [Enter].)
[PUMP] on the display is highlighted and after a while stops being highlighted. The right
plunger is now at the backmost position.
"Setting the plunger stop position《PLUNGER SET》" P.37
4
3 Attach the head holder to the pump
body.
Slowly push in the head

holder along the plunger.
Pushing in the head holder
forcibly may break the plunger.
4 Attach the 2 hexagonal socket screws to the head holder and tighten them with
an Allen wrench.
Tighten the right and left hexagon socket screws alternately in 90° increments.
Finally hold the longer part of the hex wrench and tighten them securely.
5 Attach rinse tubes to the top and bottom of the head holder.
6 In the [PLUNGER SET] screen, press [1] and [Enter].

(To replace the left plunger, press [2] and [Enter].)
plunger is now at the frontmost position.

4 Maintenance
7 Pinch the end of the plunger that is

projecting from the head holder with
your fingers and adjust its position to
make the gap (C) around the plunger
(A) inside the hole of the projection (B)
of the head holder even. While
keeping the plunger in place, wipe off
the plunger surface with clean wiper
paper soaked in 2-propanol.
Move the plunger gently.

Moving the plunger too
abruptly could scratch the
plunger surface or cause it to
break off.
8 In the [PLUNGER SET] screen, press [2] and [Enter]. (To attach the left plunger
seal, backup ring and pump head, press [1] and [Enter].)
plunger is now at the backmost position.
When replacing the plunger, be sure to replace the plunger seal and the
diaphragms."4.4 Replacing the Plunger Seal" P.92.
9 Attach the pump head, SUS pipe, and inlet tube and set the seal rinsing kit back
in place by following the instructions in "Removing the pump head" P.85.

4.6 Rinsing the Check Valve

This section will explain how to pump 2-propanol to rinse the check valve.
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
Necessary parts
Part Name Part Type Part No. Quantity

RESISTANCE TUBE,COIL Replacement
228-57908-41 1
FOR CLEANING part
1 After using buffer solution as the mobile phase, replace the solvent in the
reservoir bottle with water.
4
After using buffer solution as the mobile phase, do NOT replace with an
organic solvent such as 2-propanol without first replacing with water.
When using a buffer solution in the reservoir bottle, replace with distilled
water or purified water, and after pumping at least 20 mL into the instrument,
replace with new 2-propanol. If the buffer solution is directly replaced with
2-propanol, crystals will be precipitated, which can cause damage to the
plunger seal or cause problems with the check valve.
System Guide "Precautions When Replacing the Mobile Phase"
2 Replace the solvent in the reservoir bottle with 2-propanol.

4 Maintenance
3 Loosen the male nuts on the column

inlet and outlet with an 8-mm wrench
and then remove the coupling from
the flow path.
4 Attach the male nut (A and C) of the

coupling inlet and outlet that was
removed to the resistor tube (D), and
tighten it with an 8 mm wrench.
6 Press [Enter] once.

The cursor blinks at the [FLOW] input
position.
7 Press [2], [. ], [0] and [Enter].

[FLOW] is set to "2.0000" and confirmed.
8 Press [PUMP].
[PUMP] on the display is highlighted and delivery of 2-propanol starts at 2 mL/min.
Continue delivery at least 1 hour.
9 Loosen the male nut (A and C) of the

resistor tube inlet and outlet with an
8 mm wrench, and remove the resistor
tube (B) from the flow line.
10 Attach the removed male nut (A and

C) of the resistor tube inlet and outlet
to the coupling (D) with an 8 mm
wrench and tighten it with the 8 mm
wrench.
11 Check the pumping stability after cleaning the check valves.
"Checking the pulse《PULSE CHECK》" P.55

12 Replace the solvent in reservoir bottle with the original mobile phase.

4 Maintenance
4.7 Performing an Ultrasonic Cleaning of the Check Valve

Check valves are attached to the left and right pump heads. The following section will
explain the method for performing an ultrasonic cleaning of the check valves on the
right side (when viewed from the front). Performing an ultrasonic cleaning of the check
valves on the left side are also the same procedure.
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
1 Remove the SUS pipe and inlet tube by following the instructions in "Removing
the pump head" P.85.
2 Use a 10 mm wrench to remove the

inlet check valve (A) and the outlet
check valve (B).
Never disassemble the check valve.

Performance cannot be guaranteed if disassembled.
(For LC-40D X3 CL) Do NOT turn the inlet

check valve (A) upside down.
If you do, the internal parts will fall out.

4.7 Performing an Ultrasonic Cleaning of the Check Valve
3 Immerse the inlet check valve (A) and outlet check valve (B) into 2-propanol, and
clean for 5 minutes using an ultrasonic bath.
4 Rotate the inlet check valve (A) and

the outlet check valve (B) clockwise to
attach them to the pump head, and
after tightening by hand, use a 10 mm
wrench to tighten them as follows.
• Inlet check valve
• LC-40D X3 CL:
Hand tightening and 120° with
wrench
• LC-40D XR CL:
Hand tightening and 90° with
wrench
• Outlet check valve
• LC-40D X3 CL:
Hand tightening and 60 to 90° with wrench
4
• LC-40D XR CL:
Hand tightening and 90° with wrench
5 Attach the SUS pipe and inlet tube by following the instructions in "Installing

4 Maintenance
4.8 Replacing the Check Valve

Check valves are attached to the left and right pump heads. The following section will
explain the method for Inspection (Replacing) of the check valves.
Inspection (Replacing) of the check valves on the left side are also the same procedure.
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
Necessary parts
Target Model Part Name Part Type Part No.

LC-40D X3 CL 228-52964-42
Inlet check valve ASSY
LC-40D XR CL 228-48249-96
Consumable
LC-40D XR CL Inlet check valve ASSY, 2 pieces 228-48249-97
parts
LC-40D X3 CL 228-53334-96
Outlet Check Valve ASSY
LC-40D XR CL 228-45705-43
1 Check the pumping stability.

LC-40D CL Series Instruction Manual "3.6.4 Validation Support Information Group
(VALIDATION)/Checking the pulse《PULSE CHECK》 "
If the result of the system check is "Pass", it is considered normal.

If the result of the system check is "Fail", "4.6 Rinsing the Check Valve" P.107 . If the
pumping (pressure) is still unstable, "4.7 Performing an Ultrasonic Cleaning of the Check
Valve" P.110, and then check the pumping stability again. If the result of the system
check is "Fail", proceed to the following procedure.
2 Remove the check valve and install a new one by following the instructions in
Step 2 of "4.7 Performing an Ultrasonic Cleaning of the Check Valve" P.110.
3 Attach the SUS pipe and inlet tube by following the instructions in "Installing
4 Turn the drain valve counterclockwise to open it.
5 Press [Enter] once.

The cursor blinks at the [FLOW] input position.
6 Press [2], [. ], [0], and [Enter].

[FLOW] is set to "2.0000" and confirmed.

4.8 Replacing the Check Valve
7 Press [PUMP] and confirm the solvent continues to flow out from the drain valve.
[PUMP] on the display is highlighted and the solvent flows out of the drain tube.
There is no problem with the valve if the solvent continues to flow out.

4 Maintenance
4.9 Inspecting the Line Filter
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
1 Fix the line filter (A) with a 10 mm

wrench (B) and loosen the male nut (C)
of the pump outlet tube with an 8 mm
wrench (D) to remove it.
2 Pour water into a reservoir bottle and set the flow rate to 1 mL/min.
3 In a no load state, pump water at a rate of 1 mL/min.

• If the pressure is 0.3 MPa or less, it is considered normal.
• If the pressure is more than 0.3 MPa, replace the line filter.
When a mobile phase additive such as trifluoroacetic acid is used, a ghost peak
may be generated from the line filter. In this case, perform the following
procedure to rinse the line filter.
• Deliver a 17% phosphoric acid solution at a flow rate of 1 mL/min for about
30 minutes. (Pumping pressure: 1 MPa to 5 MPa)
For example, attach a stainless tube with ID0.1 x 2000mm (Part No.:
228-57908-41).
• Deliver purified water at a flow rate of 10 mL/min for about 30 minutes.
(Pumping pressure: 1 MPa to 5 MPa)
For example, attach a stainless tube with ID0.3 x 2000mm*1.
*1: Stainless tubing ID0.3 x 1000mm (Part No.: 228-53184-96) 2pc and Coupling
(Part No.: 228-16004-13) 1pc can be substituted.

4.10 Replacing the Line Filter
4.10 Replacing the Line Filter
Necessary tools

Wrench (8 mm and 10 mm) 2 086-03002
Necessary parts
Target Model Part Name Part Type Part No.

LC-40D X3 CL 228-57501-92
Line Filter Consumable part
LC-40D XR CL 228-35871-96

wrench (B) and loosen the male nut (C)
of the pump outlet tube with an 8 mm
wrench (D) to remove it.
4
2 Loosen the line filter (A) with 10 mm wrench to remove it.
3 Wipe with a cotton swab, etc., to wipe

away any dirt from the line filter port
(a).
4 Attach the new line filter (A) to the

line filter port (a) by hand, and tighten
it 45° using a 10 mm wrench.

4 Maintenance
5 Attach the male nut (C) of the pump

outlet tube to the line filter (A) by
hand.

wrench (B) and tighten the male nut
(C) of the pump outlet tube with an 8
mm wrench (D).
When using in the front end of

LC-MS, carry out the cleaning
process described as follows to
reduce background noise. Pump
50% methanol solution at a
pressure of approx.10 MPa for
approx. 30 min. (For example,
attach a stainless steel tube
with ID 0.1 x 2000 mm (Part
No.: 228-57908-41) and adjust
the flow rate.)

4.11 Cleaning/Replacing the Suction Filter
4.11 Cleaning/Replacing the Suction Filter
Necessary Parts

SUS Filter Element, w/PTFE
Consumable part 228-45707-91 1
Fitting
Use the SUCTION FILTER with tube and fittings for pump inlet (Part No. 228-45708-91 ) or
SUCTION FILTER with tube and fittings for the low pressure gradient kit or the degassing
unit (Part No. 228-61905-49 ) as necessary.
Contamination of suction filters can lead to contamination of mobile phase

solvents and bottles, causing background rise and ghost peaks. Even if you are
wearing gloves, do not touch the metal parts of the suction filter as much as
possible. Hold the non-metal part of the filter element.
After using the buffer solution as the mobile phase, do NOT replace with an
organic solvent such as 2-propanol without first replacing with water.
When using a buffer solution, replace it with distilled water or purified water,
and after pumping at least 20 mL into the instrument, replace it with new
2-propanol. If the buffer solution is directly replaced with 2-propanol, crystals
will be precipitated, which can cause damage to the plunger seal or cause
problems with the check valve.
1 Pull the suction filter (A) from the

suction tubing (B).
2 Place the suction filter into a clean

container with 2-propanol, and clean it
for 5 minutes using an ultrasonic bath.

4 Maintenance
3 Insert the suction tubing (B) into the

suction filter (A).
4 Replace the solvent in reservoir bottle with water, and place the suction tubing
back into the bottle.
6 Deliver water at 1 mL/min for 10 minutes.
7 Check for air bubbles inside the suction tubing.

Replace the suction filter if air bubbles are present.
If Ghost peaking occurs, rinse the suction filter. Refer to System Guide "5.3.2
Symptoms Presumably Due to an Error, Based on a Chromatogram".

4.12 Replacing the Drain Valve
4.12 Replacing the Drain Valve
Necessary tools

Phillips screwdriver 1 -
Necessary parts

DRAIN VALVE ASSY,TP Replacement part 228-51229-93
1 Rotate the drain valve ASSY counter-clockwise at least 3 complete rotations and
pull it off straight.
Check the hole for foreign objects.

4
2 Wet the seal portion (a) of the new

drain valve ASSY (A) with 2-propanol,
etc., and insert it into the hole.
If the drain valve ASSY is

forcefully inserted or is not
inserted straight, the seal
portion may become deformed,
resulting in leakage.
3 Tighten the drain valve ASSY

clockwise.

4 Maintenance
4 After tightening the drain valve ASSY,

attach the knob with the screw (B)
using a phillips screwdriver so that it
is vertical, and then attach the cap (C).
4.13 Replacing the Air Filter (Right Panel)

This section explains about replacement on the air filter of the right panel.
Necessary parts

Air Filter Element, Non Woven, for Right
Consumable part 228-53924-06
Panel
1 Open the right panel.
2 Remove the right panel cover.

While lightly pressing the cylinder-shaped
projection (a) inside the right panel cover,
slide the cover to the front.

4.13 Replacing the Air Filter (Right Panel)
3 While pressing the knob at the top of

the filter holder (A), remove it toward
the front.
4 Slide the air filter (B) to attach/remove

it.
5 Attach the filter holder and the right

panel.

4 Maintenance
4.14 Cleaning of the Leak Tray
If a leak occurred, wipe off the leak in the leak tray completely as below.
Leak of mobile phase solution used as buffer solution may dry and crystallize,
clogging the leak tray. If the leak tray is contaminated by such crystallized buffer
solution, wipe off the leak tray with paper wiper soaked in water in the same
manner as below.
1 Wipe off leak around the leak sensor or on the leak tray completely with wiper
paper.
• Do not bend or pull the leak sensor.

• If there is contamination such as crystallized buffer solution, wipe it off with
wiper paper soaked in water.
4.15 Cleaning the Exterior
4.15.1 Usual cleaning
If the exterior of the instrument is dirty, clean it with a dry soft cloth or tissue paper.
If it is very dirty, clean it as below.
1 Clean with a cloth soaked in diluted neutral detergent and wrung tightly.
2 Wipe off detergent with a cloth soaked in water and wrung tightly and then
wipe off water with a dry cloth.
Do NOT leave any part wet nor use thinner for cleaning.
Doing so may cause rust or discoloration.

4.16 Cleaning of the Automatic Rinsing Kit
4.15.2 Decontamination cleaning
After handling the hazardous substances, using the following procedure.
1 Turn off the power of the column oven.
The gas sensor may defect volatile gas generated from organic solvent.
2 Clean with a cloth soaked in Aqueous ethanol solution (80%) or aqueous sodium
hypochlorite solution (0.05%) and wrung tightly.
Do not leave metal surfaces wet.

This can cause rusting and discoloration.
4.16 Cleaning of the Automatic Rinsing Kit
Necessary parts

Replacement
WASHING PUMP SEAL KIT 228-56201-41 1
part
If the 10% 2-propanol water in the rinse solution bottle is dirty, replace with
new solution. If getting significantly dirty, replace with new solution once a day.
Even if it does not appear dirty, replace with new solution once a week.
1 Remove the rinse cap of the automatic rinsing kit from the rinse solution bottle.
2 Pour distilled water into the rinse solution botte and clean the inside of the rinse
solution bottle using a brush, etc.
3 Pour distilled water into the rinse solution bottle and rinse the inside of the
rinse solution bottle for about 5 minutes using an ultrasonic bath.
4 Pour new 10% 2-propanol water into the rinse solution bottle, and return the
cap and rinse solution bottle back to their original positions.

4 Maintenance
5 Replace the rinse tube if the inside is dirty.

5 Technical Information
5.1 Specifications
5.1.1 LC-40D X3 CL Specifications
Item Specification
Micro-volume double plunger pump
Pump Type
(approx. 10 µL/stroke)
Constant flow pumping and constant pressure
Pumping Methods
pumping
Allowable Maximum Pressure 130 MPa
Flow Rate Setting
0.0001 to 10.0000 mL/min
Range
130 MPa
Maximum Delivery (0.0001 to 3.0000 mL/min)
Pressure 80 MPa (5.0000 mL/min)
Constant Flow 22 MPa (10.0000 mL/min)
Pumping ±1 %
(1 mL/min when water is pumped at 80 MPa,
Flow Rate Accuracy
with water and room temperature constant
between 20 to 30 °C)
Flow Rate Precision

Larger value of either 0.06 % RSD or 0.02 min 5
SD
Pressure Display Accuracy Larger value of either ±2 % or ±2.4 MPa
Suction Filter 10 µm
Line Filter 5 µm
Commands for flow rate, pressure, [LOOP] (for
Time Program program repetition), 320 steps (total of 10
program files)
*1 When water is pumped at 10 to 40 MPa, with water and room temperature constant between 20
to 30 °C.
*2 When water is pumped at 40 to 60 MPa, with water and room temperature constant between 20
to 30 °C.

5.1.2 LC-40D XR CL Specifications
Item Specification
Micro-volume double plunger pump
Pump Type
(approx. 10 µL/stroke)
Constant flow pumping and constant pressure
Pumping Methods
pumping
Allowable Maximum Pressure 70 MPa
Flow Rate Setting
0.0001 to 10.0000 mL/min
Range
70 MPa
(0.0001 to 3.0000 mL/min)
Maximum Delivery 44 MPa
Pressure (3.0001 to 5.0000 mL/min)
Constant Flow 22MPa
Pumping (5.0001 to 10.0000 mL/min)
Larger value of either ±1 % or ±2 µL/min
(0.01 mL/min to 3 mL/min)
Flow Rate Accuracy
Larger value of either ±2 % or ±2 µL/min
(0.01 mL/min to 3 mL/min)
No more than the large value calculated with
Flow Rate Precision
either 0.06 % RSD or 0.02 min SD
Pressure Setting 1.0 to 60 MPa
Constant Pressure Range (in steps of 0.1 MPa)
Pumping
Pressure Accuracy Larger value of either ±10 % or 1.5 MPa
Pressure Display Accuracy Larger value of either ±2 % or ±1.0 MPa
Suction Filter 10 µm
Line Filter 5 µm
Commands for flow rate, pressure, [EVENT],
Time Program [LOOP] (for program repetition), 320 steps (total
of 10 program files)

5.1 Specifications
5.1.3 High-Pressure Gradient System
Specification
Item
Number of Solvents Mixed 2 or 3
Step, linear and exponential functions (Only when
Gradient Profile
controlled by LC workstation) possible at multiple levels
Maximum Program Steps 400 steps (total of 20 program files)
Program Duration 0.01 to 9999.90 minutes (in steps of 0.01 minutes)
Range of Set Concentrations 0 % to 100 % (in steps of 0.1 %)
±0.5 %
±0.5 %
Concentration Accuracy*1 (0.05 to 3 mL/min,
(1 mL/min, 80 MPa)
1 to 40 MPa)
Flow Rate Possible 0.0001 mL/min to 10 mL/min
*1 For binary gradient with water/caffeine solution
5.1.4 Low-Pressure Gradient System
Item Specification
Number of Solvents Mixed Max. 4
Gradient Profile
Step, linear and exponential functions (Only when controlled by 5
LC workstation) possible at multiple levels
Maximum Program Steps 400 steps (total of 20 program files)
Program Duration 0.01 to 9999.90 minutes (in steps of 0.01 minutes)
Range of Set
0 % to 100 % (in steps of 0.1 %)
Concentrations
±0.5 %(1 mL/min, 10 MPa)
Concentration Accuracy
(for binary gradient with water/caffeine solution)
Flow Rate Possible 0.0001 mL/min to 10 mL/min

5.1.5 Other Specifications
Item Specification
SUS316L, PEEK, ruby, sapphire, HastelloyC,
Liquid-Contacting Part Materials
polyethylene
Operating Temperature Range 4 °C to 35 °C
Humidity Range 20 % to 85 %
Available pH Range 1 to 14
W260 mm ´ H140 mm ´ D500 mm (Excluding
Dimensions
protruding parts)
Mass 10 kg
Power Supply Voltage 100 V AC to 240 V AC
(indicated on the * Mains supply voltage fluctuations are not to
instrument) exceed 10 % of the nominal supply voltage.
Power Consumption 150 VA
Power Supply Frequency 50/60 Hz

50 A
* Connect the instrument to a power outlet
Rated Breaking Capacity that is equipped with a circuit breaker that
shuts off the current at the described value or
less.
Installation Category Ⅱ, Pollution Degree 2,
Installation Environment (IEC) Altitude 2000 m max.
Install indoors.
Plunger Rinsing Line Automatic rinsing kit as standard equipment
Pressure Limiter Operations Upper/lower limits can be set
Exist (Error display and stop at the time of
Error Display
malfunction)

5.1 Specifications
n Low Pressure Gradient Kit
Item Specification
Flow Line Switching
Flow Line Switching with the five-way solenoid valve
Method
Number of Flow Lines 1 (with four built-in two way solenoid valve)
Solenoid Valve Operating
0 kPa to 50 kPa
Pressure
Materials for Parts
PTFE, PEEK, fluororubber
Exposed to Liquid
Operating Temperature 4 °C to 35 °C
Available Flow Rate 0.0001 mL/min to 10 mL/min

Available pH 1 to 14

5.2 Maintenance Parts

5.2.1 Consumable Parts
No. Part Name Part No. Remarks

Seal for pump head, with
PLUNGER SEAL 228-52711-93
1 backup ring
Plunger Seal GFP 228-52711-94 Optional parts
228-57501-92 LC-40D X3 CL
2 Line Filter ASSY, SUS
228-35871-96 LC-40D XR CL
3 Suction filter 228-45707-91 Filter body only (cleaned)
4 Plunger Holder ASSY 228-52069-44 with diaphragm
228-52964-42 LC-40D X3 CL
Inlet Check Valve ASSY
5 228-48249-96 LC-40D XR CL
Inlet Check Valve ASSY, 2 pieces 228-48249-97 LC-40D XR CL
228-53334-96 LC-40D X3 CL
6 Outlet Check Valve ASSY
228-45705-43 LC-40D XR CL
7 DIAPHRAGM 2PCS 228-55272-41
8 AIR FILTER FOR RIGHT PANEL 228-53924-06 Filter only

5.2.2 Replacement Parts
No. Part Name Part No. Remarks

9 PUMP HEAD 228-55230-45
10 HEAD HOLDER,2030 228-38022-01
11 Suction Filter ASSY 228-45708-91
12 PEEK Bush Fitting 3 228-39084 For suction filter
13 FERRULE,3.0F-T 228-12493 For suction filter
14 FEP TUBE 3.0 (O.D.)×1.5(I.D.) 670-10321-05 For suction filter
15 INLET BLOCK PEEK 228-47518 Pump inlet
16 DRAIN VALVE ASSY,TP 228-51229-93
17 THRUST 228-34469
For the left pump head of
228-70472-01
LC-40D X3 CL
18 SUS PIPE L
For the left pump head of
228-70026-01
LC-40D XR CL
For the right pump head of
228-70472-02
LC-40D X3 CL
19 SUS PIPE R
For the right pump head of
228-70026-02
LC-40D XR CL
20 TUBE HOLDER 228-70044
21 DRAIN TUBE ASSY 228-25495-93
For use between the inlet block
22 ETFE TUBE 1.6(O.D.)X0.8(I.D.) 228-18495-01
and the check valve
For plumbing between the inlet
23 MALE NUT 1.6 PEEK 228-35403
block and the check valve

5.2.3 Maintenance Kit
A set of consumable parts is provided as Maintenance Kit
n Maintenance Kit for LC-40D X3 CL (Parts No. 228-53265-45)
Part Name Part No. Quantity Remarks

PLUNGER SEAL,BACKUP RING UHP 228-52711-93 2 With backup ring
Line Filter ASSY, SUS 228-57501-92 1
Suction Filter ASSY 228-45708-91 1
Plunger Holder ASSY 228-52069-44 2 With diaphragm
Inlet Check Valve ASSY 228-52964-42 2
Outlet Check Valve ASSY 228-53334-96 2
Air Filter for Right Panel 228-53924-06 1
n Maintenance Kit for LC-40D XR CL (Parts No. 228-45593-49)
Part Name Part No. Quantity Remarks

PLUNGER SEAL,BACKUP RING UHP 228-52711-93 2 With backup ring
Line Filter ASSY, SUS 228-35871-96 1
Suction Filter ASSY 228-45708-91 1
Plunger ASSY 228-52069-44 2 With diaphragm 5
FERRULE 1.6F 316L 228-16000-10 2
MALE NUT 1.6MN 228-16001 2
SUS316LTP 0.3 228-50579-91 1 0.3 mm (I.D.) ´ 2 m
Inlet Check Valve ASSY 228-48249-96 2

Outlet Check Valve ASSY 228-45705-43 2

Index
A Maintenance Parts 130
Air Filter 120 Monitoring Screens 24
Operation Area 14, iv
Auto-Diagnostics 24, 25, 68 O
Automatic Rinsing Kit 6, 123 Operation Area iv
Auxiliary Functions Screen (FUNCTION) 28 Operation Panel 11
Available pH Range 128 Optional Parts 3
C P
Calibration Support Information Group [PARAMETER] Setting Group 31
(CALIBRATION) 61 password 63
CBM parameter 64 Periodic Inspection 79
Check Valve 107, 110, 112 Power Button 11
Component Parts 2 Product Information Group (PRODUCT INFO) 49
Compressibility 16, 32 Purge 14
Constant Flow Delivery Mode 20
Constant Pressure Delivery Mode 21 R
Consumable Parts 131 Replacing the Plunger Seal 92
[CONTROL] Setting Group 37
S
D Shipping Screws 10
Display Area 13 Specifications 125
Drain Valve 119 Status LED 11
Suction Filter 117
F System Controller 78
[FILE] Operation Group 46 [SYSTEM] Setting Group 38
Filter Holder 84
T
G Time Program 69
Gradient Mixer for High-Pressure Gradient Analysis tube volume 43
5
U
H [UTILITY] Setting Group 44
High-Pressure Gradient System 127
V
I Validation Support Information Group
Initializing 62 (VALIDATION) 53
VP Functions Screen 47
L
LC-40D XR CL Specifications 126
Leak Sensor 8
Line Filter 114, 115
Liquid-Contacting Part Materials 128
Low-Pressure Gradient 76
Low-Pressure Gradient System 127
M
Main Power Switch 9
Maintenance Information Group (MAINTENANCE)
49
Maintenance Kit 133

LC-40D X3 CL Lc-40D XR CL: Instruction Manual

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228-97197B

Solvent Delivery Module for Shimadzu

Document Name Document No. Description

Read "Safety Guideline" thoroughly before using the product.

ã 2020-2022 Shimadzu Corporation. All rights reserved.

Indications Used in This Manual

Indicates a potentially hazardous situation which, if not

Indicates a potentially hazardous situation which, if not

The following symbols are used in this manual:

Indicates an action that must not be performed.

Indicates an action that must be performed.

Indicates information provided to improve product performance.

Indicates the location of related reference information.

n EN55011 Emissions (Electromagnetic Interference)

This is a class B product. When this product causes an electromagnetic disturbance to

Operating Nexera CL System

This system is designed to perform qualitative and quantitative analysis of target

A calibration curve should be generated for each analyte in appropriate methods. At

LC-40D X3 CL LC-40D XR CL iii

1 Overview 1.1 Features ............................................................. 1

2.2 Behind Front Cover ............................................. 8

2.5.2 Operation Area ............................................... 14

3 Operation 3.1 Settings for the Basic Operation ......................... 16

3.1.2 Setting Flow Rate ............................................. 16

3.1.5 Setting Minimum Pressure Limit .............................. 18

3.2 Operation ......................................................... 19

3.2.2 Operation in Constant Flow Delivery Mode .................. 20

3.2.3 Operation in Constant Pressure Delivery Mode .............. 21

3.5.3 [CONTROL] Setting Group .................................... 37

3.5.6 [FILE] Operation Group ........................................ 46

3.6 VP Functions Screen ........................................... 47

3.6.5 Calibration Support Information Group (CALIBRATION) ...... 61

3.7 Creating Time Program ...................................... 69

3.7.2 Time Program Setting Screen ................................. 70

3.7.3 Creating a Time Program ..................................... 71

3.7.4 Creating a Low-Pressure Gradient Program .................. 73

3.8.2 Preparation for Delivery ....................................... 76

3.9 Connection with the System Controller or

4 Maintenance 4.1 Periodic Inspection and Maintenance .................. 79

4.1.2 List of Periodic Inspection and Maintenance ................. 82

4.1.3 Check after Inspection and Maintenance .................... 83

4.4 Replacing the Plunger Seal ................................ 92

4.4.2 Resetting the Delivery Amount ............................... 99

4.15.2 Decontamination cleaning .................................. 123

4.16 Cleaning of the Automatic Rinsing Kit .............. 123

5.1.1 LC-40D X3 CL Specifications ............................... 125

5.1.4 Low-Pressure Gradient System .............................. 127

5.2.3 Maintenance Kit ............................................. 133

Index ......................................................... 134

LC-40D X3 CL LC-40D XR CL vii

n Low Flow Pulsation and Pulsation Period Enable Precise Delivery

n Choice of Gradient Modes and Control Options

n Automatic Plunger Rinsing

An automatic rinsing kit is provided as standard for LC-40D X3 CL/LC-40D XR CL . It can

n High-pressure Flow is Available

1.2 Component Parts

No. Part Name Part No. Q'ty Remark

*1 It contains cautions for use regarding the instrument.

1.3 Optional Parts