ORIGINAL ARTICLE J Nepal Med Assoc 2023;61(268): 931-3

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doi: 10.31729/jnma.8363

Human Papillomavirus among Women Undergoing Papanicolaou Smear

Test in the Department of Gynaecologic Oncology of a Tertiary Care
Centre
Mahendra Raj Shrestha,1 Ajaya Basnet,2 Rajendra Maharjan,1 Sagar Ghimire,1 Nisha Khatri,3
Arju Shrestha,3 Lochan Karki,4 Saujanya Karmacharya3
1
Department of Clinical Laboratory, Nepal Armed Police Force Hospital, Balambu, Kathmandu, Nepal,
2
Department of Microbiology, Nepal Armed Police Force Hospital, Balambu, Kathmandu, Nepal, 3Department
of Gynaecologic Oncology, Nepal Armed Police Force Hospital, Balambu, Kathmandu, Nepal, 4Department of
Medicine, National Academy of Medical Sciences, Mahaboudha, Kathmandu, Nepal.

ABSTRACT

Introduction: In invasive cervical specimens or precursors, high-risk human papillomavirus

Deoxyribonucleic acid may be detected to identify females at risk of developing cervical cancer.
This study aimed to find out the prevalence of human papillomavirus among women undergoing
Papanicolaou smear tests in a tertiary care centre.

Methods: A descriptive cross-sectional study was conducted among women undergoing the
Papanicolaou smear test in the Department of Gynaecologic Oncology, Nepal Armed Police Force
Hospital, between 1 June 2022 and 15 November 2022. Ethical approval was obtained from the
Ethical Review Board. A convenience sampling method was used. The point estimate was calculated
at a 95% Confidence Interval.

Results: Among the 199 women, 6 (3.02%) (0.64-5.40, 95% Confidence Interval) had human
papillomavirus infection. The mean age of the infected females was 31.17±5.57 years. Human
papillomavirus DNA for 16 and 18 were detected in 4 (66.67%) and 2 (33.33%) females, respectively.

Conclusions: The prevalence of human papillomavirus in females was found to be lower than other
studies done in similar settings.

Keywords: cytology; histology; human papillomavirus; Nepal; prevalence.

INTRODUCTION
statistics in cancer prevention programs across the
Human papillomaviruses, the double-stranded DNA country. However, scarce literature exists concerning
papovaviruses, cause cervical cancer-the second most cervical cancer in Nepal.
common and the fifth most lethal cancer in women
worldwide.1,2 In 2020, global cervical cancer incidence This study aimed to find out the prevalence of
and mortality rates were estimated at 13 per 100,000 human papillomavirus among women undergoing
women and 7 per 100,000 women, respectively, Papanicolaou smear tests in a tertiary care centre.
compared to 16 and 11 per 100,000 women, in Nepal,
making it an extremely dangerous disease.3 METHODS

Although cervical cancer is highly prevalent in Nepal, A descriptive cross-sectional study was conducted
very few studies have evaluated the prevalence of among women undergoing the Papanicolaou smear
HPV in precursor lesions and invasive cancer. As test in the Department of Gynaecologic Oncology,
Nepal has subtle regional and cultural differences, it ______________________________________
is vital to describe the prevalence of HPV in cervical Correspondence: Mr Ajaya Basnet, Department of
intraepithelial lesions and invasive cancer in multiple Microbiology, Nepal Armed Police Force Hospital, Balambu,
representative populations to generalize these Kathmandu, Nepal. Email: [email protected], Phone: +977-
9841170737.

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 Shrestha et al. Human Papillomavirus among Women Undergoing Papanicolaou Smear Test in the Department of Gynaecologic Oncology...

Nepal Armed Police Force Hospital, between 16 May smears with abnormal epithelial cells. Gynecologic
2022 and 15 November 2022. Ethical approval was oncologists collected cells from the uterine cervix
obtained from the Ethical Review Board (Reference in a sample collection buffer (5 ml) and sent them
number: 20790202). Individuals who were >18 years old to the molecular laboratory for HPV DNA testing.
who visited the hospital's Department of Gynaecologic DNA extraction kits were used as directed by the
Oncology for routine examinations and giving consent manufacturer (SpinStarTM Viral Nucleic Acid Kit 1.0,
were included in the study. Individuals with incomplete ADT Biotech). The Human Papillomavirus Nucleic Acid
study details and who did not give informed consent Amplification Test Kit (Fluorescent Probe-Based Real-
were excluded. A convenience sampling method Time PCR Assay) (Solis BioDyne) was used to detect
was used. The sample size was estimated using the HPV-16, HPV-18, and other types. HPV positivity was
following formula: assessed by real-time polymerase chain reaction.
pxq
n= Z2 x Data were collected and entered in Microsoft Excel
e2
2010 and analyzed in IBM SPSS Statistics version 17.0.
0.50 x 0.50
= 1.962 x Point estimate and 95% CI were calculated.
0.072
= 196 RESULTS
Where, Among 199 women, HPV infections were detected in 6
n= required sample size (3.02%) (0.64-5.40, 95% CI). HPV-infected women had
Z= 1.96 at 95% confidence interval (CI) a mean age of 31.17±5.57 years. Among the females
p= prevalence taken as 50% for maximum sample size infected with HPV, DNA for genotype 16 was detected
calculation in 4 (66.67%) (Figure 1).
q= 1-p
e= margin of error, 7%
The calculated minimum required sample size was
196. However, a total of 199 patients were included in
the study.

The patient information sheet recorded demographic,

cytological and molecular (HPV-16, HPV-18, and others)
findings. The informed consent of participants was
obtained, and records that were missing or ambiguous
were clarified by contacting involved healthcare
workers and/ or the participants themselves. The
gynecologic oncology team collected specimens from
the cervical os using an endocervical swab, fixed them Figure 1. Human papillomavirus genotypes (n= 6).
with a fixative spray, and sent them to the cytohistology
A total of 2 (33.33%) cases of low-grade squamous
laboratory for cytohistological analysis. A fixed smear
intraepithelial lesions (LSIL) and 2 (33.33%) cases of
was prepared in the cytohistology laboratory and
high-grade squamous intraepithelial lesions (HSIL)
immersed in tap water for 3 minutes. Excess water
were observed among females infected with HPV-
was blotted off the slide. For 60 seconds, the slide
16. On the other hand, 1 (16.67%) case of HSIL and 1
was dipped in rapid-pap nuclear stain (hematoxylin
(16.67%) case of squamous cell carcinoma (SCC) were
solution). Next, the slide was washed after ten
observed among females infected with HPV-18.
seconds by adding three drops of Scottie's tap water
buffer. Following that, the slide was immersed for 30 DISCUSSION
seconds in rapid-pap dehydrant (propranolol) with two
solution changes. The slide was dipped in a working The prevalence of HPV infections in females in this
cytoplasm stain (og-6 solution, light green SF-eosin) study was 6 (3.02%), which was lower than in studies
for 45 seconds. The slide was washed in tap water, conducted in India (15–85%).5 This study involved
and excess water was blotted away. Afterwards, a hospital-visiting females, often paramilitary, rather
rapid-pap dehydrator was used to dehydrate the slide than community-residing females, as in other studies,
for 30 seconds and air-dry. The slide was then dipped which resulted in a lower prevalence of HPV infections.
in xylene, dried, and mounted with dibutyl phthalate A previously published study from Nepal showed
polystyrene xylene using a coverslip. Lastly, the smear higher HPV-16 (72.20%) infection rates than HPV-
was reported according to the revised 2001 Bethesda 18 (14.80%), though with varying incidence rates.6 A
system.4 HPV DNA testing was performed only on variety of factors influence HPV prevalence, including

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 Shrestha et al. Human Papillomavirus among Women Undergoing Papanicolaou Smear Test in the Department of Gynaecologic Oncology...

age of sexual debut, number of partners, parity, use of circumcision, and personal hygiene.12,13 Studies have
oral contraception, and socioeconomic status.7 also shown that females over 40 years have the highest
incidence of invasive cancer, which peaks around age
In our study, the mean age of the infected females 50 and precursors are detectable for up to 10 years
was 31.17±5.57 years. Furthermore, a study involving before cancer develops, with a peak lesion rate at 35
361 cervical specimens found that high-risk HPV was years of age.12
most prevalent in females between the ages of 15 and
35 years.8 Researchers have linked HPV infection in As this study involved only one centre, no generalization
females to an early age of intercourse, an insufficient of the study findings could be made for the entire
adaptive immune response, and relative incompetence nation, therefore further studies involving multiple
in viral clearance because of hormonal changes during centres and a larger number of cervical specimens are
the menopausal transition, which may lead to latent required.
HPV infection persistence or reactivation.9
CONCLUSIONS
The present study showed the highest number of
HSIL cases among those infected with HPV, followed The prevalence of HPV infections in females was lower
by LSIL (33.33%) and SCC (16.67%). Similarly, studies than in national and international studies performed
conducted in low- and middle-income countries have in similar settings. Formulation of cervical cancer
shown higher cases of LSIL (41-77.7%) than HSILs (5.2- prevention guidelines including screening, and HPV
14%).10,11 In this study, it was equally predominant to vaccinations could be promoted to decrease the
detect HPV-16 in females with LSIL and HSIL, and HPV- burden of cervical cancer.
18 in females with HSIL and SCC. Most likely, this is
Conflict of Interest: None.
due to cultural differences, including age at marriage,
number of sexual partners, religious practices such as REFERENCES

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