1-Medical Bio 1 (Lab Safety - 23-24)

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Medical Biology

Lab -1 -
objectives
• Define Lab , Safety , And Lab Safety .
• Types of safety (Personal , Room , Equipment)
• What To Do And Not To Do In The Lab.
• The General Hazards In A Laboratory .
• Understand The First Aid In Case Of Any
Accident.
• General Arrangement .
• Waste Materials
• Biological Safety Levels
2
What is laboratory
• Laboratory is a building
or room equipped for
conducting scientific
research or for teaching
practical science .
• It is a workplace for the
conduct of scientific
research
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safety
• ‪Safety
is the state
of being "safe",
the condition of
being protected
from injury or
other dangers.
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So what is lab safety ?
Its procedure that used to
protect your self ,lab, your
reagent and equipment.

That mean the laboratory safety


classify into:
1. Personal safety
2. Room and environment safety
3. Reagent and equipment safety
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This is a ways that use to protect your self and your team work it
include:
1. Wearing lab coat is to protect your self from the chemical
material and the contaminated sample
2. Wearing gloves to protect against sharpener contaminated
samples and chemicals
3. Wearing mask when required (for inhaling pathogen)
4. Don't eat or drink in side the lab
5. Wash the hand before and after work by detergent soap
6. Don't put your personal material in the working pinch (Mobil, bag )
7. Use the suitable light for examination to reduce the eye effect
Personal Protective Equipment (PPE)
General rules for using PPE:
•Make sure it fits properly
•Check for damage before use
•If damaged during use,
remove and replace
immediately
•Do not wear sandals or open
shoes
•Remove PPE when leaving the
laboratory
To make the room as suitable place:
1. It must contain the hazard door
2. It have a good ventilation
3. Contain water supply
4. Contain existent fan
5. Good roof and good flour
6. Lab Room include :
 safety shower
 eye wash station
 fire extinguisher
 fire blanket
 fire alarm
 first aid
To keep your reagent and equipment in the perfect way
from sun , dust and bad effect .Any chemical that should be
consider as hazard chemical and you must wear gloves, and
eye goggles

Laboratory safety equipment The equipment preserve from


environment effect Some equipment can effect by low or
high temperature Some can be effected by different type of
chemical .
Protecting yourself
what to do ???
• Wear protective clothes
(Laboratory coats )
• Don’t wear sandals or open
shoes
• Long hair must be tied back
• Use protective gloves
• Remove your gloves before
using instruments, telephone,
and leaving the laboratory
• Always wash your hands
before you leave and
especially before eating
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What not to do???
• Never eat, drink or
smoke in a laboratory
• Never apply cosmetics
• Never touch your face,
mouth or eyes
• Never suck pens or
chew pencils
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• Fire
• Breakage of
glassware
• Sharps
• Spillages
• Pressure equipment
& gas cylinders
• Extremes of heat &
cold
• Chemical hazards
• Biological hazards
• Radiation
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In case of an emergency
• Know the location of :
• First aid kit
• Fire extinguisher
• Fire blanket
• Eyewash station
• The exit doors

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First aid
The following measures are given as first aid
possibilities until arrival of medical assistance.
- Eye injuries with corrosive substances Wash
the eyes with a large amount of water (eye
showers, douche) for approx. 10 min. Maintain
the eyelid open with the help of the fingers.
- Eye injuries with foreign parts Do not
administer first aid, wait for a trained
professional.
- Skin chemical burns Remove immediately the
contaminated clothes. Wash thoroughly the
wounded body parts using the emergency
showers .Cool the burned area with cold water
for approx. 10 min. Never use cream or oil.
• Toxic gas inhalation Close the toxic
gas source without endangering
yourself. Move the casualties away
from the danger zone.

• Opened wounds and bites Cover with


sterile bandage. (first aid kit ).

• Ingestion of chemical substances


Rinse the mouth thoroughly with
water, do not give any antidote
emetics .

• Hemorrhages Stop the bleeding by


placing the wounded limb in an
elevated position, press with your
finger or first stop the hemorrhage,
and cover the wound with sterile
bandage (first aid kit).
 Fractures Reassure the person;
do not move the broken limb.

 Electrocutions Turn of electrical


power (turn out the fuse, located
in the corridors!). In case of
respiratory arrest, start artificial
ventilation.

 Breathing stops Immediately


perform the artificial respiration

 .
General
Arrangement X
 Keep your workplace
tidy
 Clear up waste, deal
with washing up and put
things away as you
finish with them
 Make sure everything is
safe before you leave
things
 a cleanly laboratory
avoids accidents to
everyone 17
Waste Materials
• Part of your risk assessment will be to
determine how to dispose of waste lab
materials safely
• Ask your supervisor or a senior if you do not
know the correct disposal method for the
materials with which you are working
• Sharps contaminated with biologically
hazardous materials, syringes and needles
must be collected in special containers to be
sent for incineration.
• Solvents and oils must be segregated into the
correct waste bottle or drum
• Your department will help you determine what
to do with chemical or biological materials
• Do not put materials down the drain or in with 18
normal waste unless authorized to do
Biosafety is the development of a system to
prevent the transmission of biological agents to
workers, other persons or the environment.
So there are 4 levels :
BSL-4
Very serious disease for humans, animals and plants.
BSL-3
Serious disease for humans, animals and plants.
BSL-2
Moderate risk to personnel and the environment.
BSL-1
Low risk for personnel and the environment.

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