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    Take Care - Bio

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    The protein particle will migrate toward the cathode during electrophoresis. At pH 5.0, which is greater than the protein's isoelectric point of 4.0, the protein will have a net negative charge and migrate toward the positively charged cathode.

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    Take Care - Bio

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    The protein particle will migrate toward the cathode during electrophoresis. At pH 5.0, which is greater than the protein's isoelectric point of 4.0, the protein will have a net negative charge and migrate toward the positively charged cathode.

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    The protein particle will migrate toward the cathode during electrophoresis. At pH 5.0, which is greater than the protein's isoelectric point of 4.0, the protein will have a net negative charge and migrate toward the positively charged cathode.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    2 views9 pages

    Take Care - Bio

    Uploaded by

    fyou56939
    The protein particle will migrate toward the cathode during electrophoresis. At pH 5.0, which is greater than the protein's isoelectric point of 4.0, the protein will have a net negative charge and migrate toward the positively charged cathode.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 9

    Take care

    Bio
    1- Which of the following equation is needed for
    calculation of serum albumin level?
    A. (Absorption of sample/absorption of standard) concentration of standard
    B. (Adsorption of test /adsorption of standard) concentration of standard
    C. (Absorbance of sample / absorbance of standard) concentration of standard
    D. (Density of test/density of standard) concentration of standard

    2- Spectrophotometry measures:

    A. Absorbance
    B. Adsorption
    C. Density
    D. d. Absorption
    : ‫ من البلوك اللي فات‬Principles of spectrophotometer ‫من محاضرة ال‬
    3-What does the electrophoresis apparatus consist of?
    a) Gel, buffer chamber and fire pack
    b) Buffer chamber and electrophoresis unit
    c) Electrophoresis unit and gel separator
    d) Power supply and electrophoresis unit

    4- Which of the following is responsible for synthesis of


    Immunoglobulins?
    a. Liver
    b. Spleen
    c. plasma cells
    d. Bone marrow
    Most plasma proteins are synthesized by the liver. Exceptions to this
    include the immunoglobulins, which are secreted by B- lymphocytes known
    as plasma cells.
    Hypoproteinemia :
    1. Decrease synthesis: Liver
    5- Hypoalbuminemia could be carried by: disease and protein calories
    malnutrition.
    A. Decrease synthesis in liver. 2. Increased loss from:
    B. Severe burns.  Kidney: nephrotic syndrome.
    C. nephrotic syndrome  Skin: burns.
    D. All of the above.  Intestine: malabsorption.
     Blood: wounds and bleeding.

    6-Which of the following conditions could leads to Hyperproteinemia?

    A. Dehydration Hyperproteinemia :
    B. burns 1- Excessive production (γ-Globulin) called
    C. hemorrhage hypergammaglobinemia causes:
    D. Liver disease • Multiple myeloma (malignant tumor of plasma cells)
    • Chronic inflammations
    • Autoimmune diseases (systemic lupus
    erythematosis).
    2- Dehydration: only concentration changes not
    changes in absolute amount.
    7-When an analyte is present in a sample, the biuret color will turn:

    a. Violet

    b. purple

    c. green

    d. yellow

    8- The biuret reagent is used to test material for the presence of :


    In the presence of Biuret reagent which contains
    a. protein
    an alkaline cupric sulfate, the protein produces a
    b. carbohydrates violet color (Chelate between Cu+2 ions and
    peptide bond NHCO) the intensity of which is
    c. nucleic acids proportional to (the number of peptide bonds at
    least 2 peptide bonds is needed) the
    d. lipids concentration of proteins.
    9-Which of the following structure is identified by biuret test?

    A. amino group
    B. carboxyl group
    C. peptide bound
    D. Hydrogen

    10- Which of the following protein is present in the prealbumin zone in


    electrophoresis?
    A. Transthyretin
    B. Ceruloplasmin
    C. Haptoglobin
    D. Immunoglobulin

     The albumin fraction also includes transthyretin [TTR] (originally


    called prealbumin or thyroxine-binding prealbumin because it ran faster
    than albumin on electrophoresis gels), which together with other proteins
    transports the hormone thyroxine and its metabolites.

    11-What happened to total protein if the collected blood


    sample was Hemolyzed?
    A. Increased
    B. Decreased
    C. Not affected
    D. Reach zero level
    hemolysis will falsely elevate total proteins because of the release of RBCs
    proteins into serum.
    12-Which of the following is true about serum albumin level
    in patients with liver cirrhosis?
    A. Its level is increased due to decrease excretion
    B. Its level is increased due to decrease metabolism
    a. c. its level is decreased due to excess loss
    C. Its level is decreased due to decrease synthesis

    Hypoalbuminemia:
    Reduced Synthesis:

     Chronic liver disease:

     The increase of globulins that occurs in early cirrhosis, will balance the
    decrease in albumin to give a total protein concentration within acceptable
    limits.

     Normal A/G value is 2:1.

     A/G ratio is the most sensitive hepatomarker. Inverted ratio indicates


    decreased albumin in chronic liver disease.

    13-The speed of migration of ions in electric field depends on:

    a. Shape and structure

    b. Amino acids and size

    c. Charge and shape

    d. Mass of molecule

    14-Which of the following ranges is considered normal for a human serum albumin
    test?

    a. 3.5-5.5 g/dl

    b. Zero -5g/dl

    c. 5-10 g/dl

    d. 2.4-3.4 g/dl
    15-Which of the following is the function of human serum albumin?

    a. Prevent the fluid part of blood from leaking into surrounding tissues

    b. Prevent the movement of foreign substances through the bloodstream

    c. Fight disease causing agents such as viruses or bacteria

    d. Prevent fluids produced by major organs from entering the bloodstream

    16- The normal reference range for total plasma proteins are:

    a) 2.5-4.5 g/dl
    b) 4.5-6.0 g/dl
    c) 6.0-8.2 g/dl
    d) >8.3gm/dl

    17-Which of the following protein is present in the gamma-globulin fraction?


    a) Transthyretin
    b) Ceruloplasmin
    c) Haptoglobin
    d) Immunoglobulin

     The albumin fraction also includes transthyretin [TTR] (originally


    called prealbumin or thyroxine-binding prealbumin because it ran faster
    than albumin on electrophoresis gels), which together with other proteins
    transports the hormone thyroxine and its metabolites.

    18-Which of the following is not the function of albumin?


    a) Maintenance of osmotic pressure
    b) Binding and transport of fatty acids and bilirubin
    c) Transport of iron
    d) Transport of drugs such as sulphonamides
    19- The following condition can be altered A: G (albumin/globulin) ratio less than 2
    with decreased plasma albumin concentration:
    a) Chronic hepatic Cirrhosis
    b) Anemia
    c) Obstructive jaundice
    d) Increased alcohol consumption

    20-Which technique separates charged particles using electric field?


    a) Hydrolysis
    b) Electrophoresis
    c) Protein synthesis
    d) Protein denaturing

    21-The protein electrophoresis pattern of a plasma sample reveals a peak in the


    region containing the most abundant plasma protein which is:

    A. Alpha globin

    B. Beta globin

    C. Gamma globin

    D. Albumin

    22-Serum albumins and globulins can be separated by the method of:

    A. Electrophoresis

    B. Polarography

    C. Dialysis

    D. Spectrography
    23- Doctor, before prescribing of parenteral protein nutrition made laboratory
    study electrophoretic of spectrum of plasma proteins. What physicochemical
    properties of proteins are used in this method?

    A. Ability to be charged

    B. Viscosity

    C. The inability to denaturate

    D. Hydrophility and swelling

    24- Which electrode to the protein particle will move during electrophoresis, if its
    isoelectric point is 4.0, and the pH for buffer solution is 5.0?

    A. Anode

    B. Calomel electrode

    C. Cathode

    D. Silver electrode

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