Scribd Logo
Upload

Welcome to Scribd!

  • 41 views

    Histology

    Uploaded by

    Alliana Florenza
    This document provides an introduction to histology and the process of tissue processing. It discusses that histology is the study of tissues, their structure and function, and how they combine to form organs. It then explains the four basic types of tissues - epithelial, connective, muscular and nervous tissues. Finally, it outlines the key steps involved in processing tissue for histological examination, including fixation, dehydration, clearing, infiltration, embedding, sectioning, staining and mounting. The goal of these processing steps is to preserve tissue structure and allow thin sections to be viewed microscopically.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Histology

    Uploaded by

    Alliana Florenza
    41 views65 pages
    This document provides an introduction to histology and the process of tissue processing. It discusses that histology is the study of tissues, their structure and function, and how they combine to form organs. It then explains the four basic types of tissues - epithelial, connective, muscular and nervous tissues. Finally, it outlines the key steps involved in processing tissue for histological examination, including fixation, dehydration, clearing, infiltration, embedding, sectioning, staining and mounting. The goal of these processing steps is to preserve tissue structure and allow thin sections to be viewed microscopically.

    Original Title

    histology

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document provides an introduction to histology and the process of tissue processing. It discusses that histology is the study of tissues, their structure and function, and how they combine to form organs. It then explains the four basic types of tissues - epithelial, connective, muscular and nervous tissues. Finally, it outlines the key steps involved in processing tissue for histological examination, including fixation, dehydration, clearing, infiltration, embedding, sectioning, staining and mounting. The goal of these processing steps is to preserve tissue structure and allow thin sections to be viewed microscopically.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    41 views65 pages

    Histology

    Uploaded by

    Alliana Florenza
    This document provides an introduction to histology and the process of tissue processing. It discusses that histology is the study of tissues, their structure and function, and how they combine to form organs. It then explains the four basic types of tissues - epithelial, connective, muscular and nervous tissues. Finally, it outlines the key steps involved in processing tissue for histological examination, including fixation, dehydration, clearing, infiltration, embedding, sectioning, staining and mounting. The goal of these processing steps is to preserve tissue structure and allow thin sections to be viewed microscopically.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 65

    INTRODUCTION TO HISTOLOGY

    and
    TISSUE PROCESSING
    WHAT IS
    HISTOLOGY?

    Is the study of tissues, their functions and


    arrangement to constitute an organ.

    Branch of Anatomy = also known as


    Microscopic anatomy
    What is a Tissue?
    A tissue is a group of cells WITH INTERRELATED FUNCTION , not just
    GROUP OF CELLS.

    Cells in the tissue does not need to be the same type, or same
    morphology.
    NERVOUS
    TISSUE EXAMPLE

    Take note that the


    cells are not of the
    same type, nor the
    same morphology and
    function. But since
    they are a group of
    cell with interrelated
    function, they can be
    called a tissue.
    Why study
    cells in a Since tissues are
    subject that is made up of cells,
    supposed to you can never
    study understand the
    Tissues? characteristics of a
    tissue without
    understanding the
    characteristics of
    its individual cell.
    4 Basic types of If you combine these four basic types of tissue, you can
    form an organ.
    Tissue
    HCl
    Epithelial
    tissues are
    AVASCULAR.
    If the epithelial
    tissue in
    stomach is
    avascular, how
    can it get its
    oxygen and
    blood supply?
    Answer:
    The connective tissue
    below is highly
    vascularized, to which it
    shares blood supply and
    oxygen to the tightly
    packed epithelial tissue.
    Remember that
    EPITHELIAL
    TISSUE and
    CONNECTIVE
    TISSUE are
    lovers.
    Remember that
    EPITHELIAL
    TISSUE and
    CONNECTIVE
    TISSUE are
    lovers.
    Connective tissues
    are responsible for
    connecting the
    epithelial tissues to
    the muscle tissue,
    hence the name
    CONNECTIVE tissue.
    Take note:
    Tissues are not
    always tightly
    packed with cells
    but rather is also
    composed of Fibers
    and Ground
    substances.

    Fibers and Ground


    substances are
    referred to as
    EXTRACELLULAR
    MATRIX
    IF ONLY
    HISTOLOGY
    SPECIMEN
    LOOKS LIKE
    THIS, IT
    WOULD BE
    MUCH EASIER
    TO IDENTIFY
    THE CELLS,
    THE FIBERS,
    AND THE
    GROUND
    SUBTANCE.
    TI O N
    E C TA
    EX P
    REALITY
    HOW CAN A
    TISSUE
    TURN FROM
    THIS?
    TO THIS?
    In the Lab, we view specimens under a microscope.
    The tissue specimen undergoes various
    In order to make a histology slide:
    steps of tissue processing.
    STEPS IN TISSUE PROCESSING:
    WHY IS FIXATION
    IMPORTANT?
    UNFIXED
    FIXED After the specimen
    is surgically
    removed from the
    body, it should be
    preserved of fixed
    with a FIXATIVE to
    prevent the decay of
    the tissue.
    IMAGINE CUTTING A
    TISSUE THAT IS AS SOFT
    AS A GELATIN, IT’LL BE A
    TOUGH JOB.
    Decalcification is NOT only limited to
    bones and teeth.
    WHY IT SHOULD BE ASCENDING GRADES OF ALCOHOL?
    TO PRESERVE THE MORPHOLOGY AND APPERANCE OF THE ORGAN.
    INFILTRATION
    and
    PLASTIC Since paraffin wax requires the use of oven, some
    Medical Technologist are reported to experience burns
    RESINS from the melted paraffin.

    Plastic resins may be used but is TOO EXPENSIVE.


    TRADITIONAL
    PARAFFIN
    WAX AFTER
    EMBEDDING
    STANDARD ROUTINE TISSUE PROCESSING
    • FIXATION
    • DECALCIFICATION – IF CALCIFIED SPX
    • DEHYDRATION
    • CLEARING
    • INFILTRATION
    • EMBEDDING
    • SECTIONING
    • STAINING
    • MOUNTING
    TURN AROUND TIME IS 5 DAYS.
    What if there’s a faster way to do biopsy?

    There is a faster but more expensive


    way to do biopsy however it is by
    case to case basis and that
    is…FROZEN SECTION BIOPSY.
    NOT THIS ONE
    END OF LECTURE!

    You might also like