MIBI-Fly CHROMagar Orientation-E

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CHROMagar™ Orientation

For isolation and differentiation


of urinary tract pathogens
CHROMagar™ Orientation
For isolation and differentiation of urinary tract pathogens

Background
Urine analysis is the most common clinical microbial test.
For instance, in France in 2007, out of 10 million bacteriology tests carried out, 6 million
(60 %) were urine analyses. Thus, any workload reduction related to this analysis will dramati-
cally improve the efficiency of the laboratory.

Medium Performance
Plate Reading
1 Instant palette of colours to obtain a large spectrum of
species differentiation
• E.coli CHROMagar™ Orientation has several advantages over traditional media:
→ dark pink to reddish - allows in most cases full differentiation of the pathogens
- allows for reliable detection semi-quantitative and presumptive identification of
urinary tract pathogens
• Enterococcus
→ turquoise blue - easier recognition of mixed growth
- provides higher detection rates

• Proteus 2 High detection of minor population


→ brown halo The proper use of CHROMagar™ Orientation will correctly pinpoint the presence
of a minor population and will help to establish the right diagnosis and therapy.
• Klebsiella,
Enterobacter, 3 Save time and reduce workload
Serratia
→ metallic blue
The most common UTI pathogen is E. coli, found in 40-70 % of infections.
CHROMagar™ Orientation has a specificity of 99,3 %* for E. coli, rendering the
• S.aureus species confirmatory test largely unnecessary.
→ golden, opaque, small Merlino, J. et al. 1996. Evaluation of CHROMagar TM Orientation for Differentiation and Presumptive Identification of Gram-
Negative Bacilli and Enterococcus Species, J.C.M. 34: 1788-1793.

One plate of CHROMagar™ Orientation will give the same information as the
• Citrobacter
→ metallic blue
combination of the 3 classical plates used for UTI analysis (blood agar, CLED and
with red halo MacConkey agar). Moreover, since it is easy to differentiate mixed flora on CHRO-
Magar™ Orientation, antimicrobial susceptibility tests can be performed directly
from primary isolates without the need of subcultures.

CHROMagar is a trademark of Dr A. Rambach


• S.saprophyticus *Samra, Z et al. 1998. Evaluation of use of a new chromogenic agar in detection of urinary tract pathogens, J.C.M. 36:
→ pink, opaque, small 990-994

4 Isolation of a variety of Microoganisms


• Candida albicans The major target of this medium is the detection of urinary tract pathogens but
→ colourless CHROMagar™ Orientation has a broader application when supplemented with
various antibiotics in detecting increasingly important nosocomial and multidrug
resistant microorganisms (See CHROMagar™ ESBL and CHROMagar™ KPC).
• Streptococcus agalactiae
→ Light blue
CHROMagar™ can also be used to differentiate various microorganisms in envi-
• Pseudomonas ronmental field.
aeruginosa
→ Translucent,
cream to blue Medium Description
Version 7.0 / Jun -20 (Mast)

Powder Base Total ..................................................................... 33 g/L


Agar..................................................................... 15.0
Peptone and yeast extract ................................. 17.0
Chromogenic mix.................................................. 1.0
Storage at 15/30 °C - pH: 7.0 +/-0.2
Shelf Life .................................................. > 18 months

Usual Samples urine, surfaces, air, clinical and other materials.


Procedure Direct Steaking. Incubation at 35-37 °C, 18-24 h.
LF-EXT-002

Manufacturer: Aerobic condition.


CHROMagar
Scientific Publications on this product: available on www.CHROMagar.com
4 place du 18 juin 1940 75006 Paris - France
Please read carefully the instructions for use (IFU document) available on
e-mail: [email protected]
www.CHROMagar.com
www.CHROMagar.com

Distributed by: Tel: +49 (0)4533 2007 0 Ordering Information


Mast Diagnostica GmbH Fax: +49 (0)4533 2007 68
Product Order Code
Feldstraße 20 e-mail: [email protected]
DE-23858 Reinfeld www.mast-group.com CHROMagar™ Orientation dry media, 5 liter 15RT412
CHROMagar™ Orientation ready to use plates, 20 pcs. 201410

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