Biochem Lab Papers
Biochem Lab Papers
Biochem Lab Papers
BSMT2C
M1 Pre-Task DONE
Type one definition/description /property/ example of an acid and a base that you could recall using the
format below.
An acid contains a hydrogen atom and dissolves in water to form a Hydrogen ion,
Acid H+. Acids react with bases to produce a salt compound and water. Hydrochloric
acid is an example of an acid.
A base contains hydroxide and dissolves in water to form OH -. Bases change the
Base
color of litmus from red to blue. Sodium hydroxide is an example of a base.
Let us check if you really read the discussions. Take the 5-item quiz before proceeding to Lesson 2.
Let us see how well you understood our discussion on Buffers. Take this 5-item quiz to assess what you
have learned from Lesson 2.
Group work:
M2 Pre-Task DONE
https://uwaterloo.ca/chem13-news-magazine/december-2016-january-2017/feature/buffered-vs-non-
buffered-solution
In the non-buffered solution, the added hydronium or hydroxide ions have nothing to reach with so the
concentrations increase rapidly, changing the pH significantly. Therefore, there is a significant change in
the solution as seen in the change of color. From yellow, it became orange after adding 1 drop of 1M HCl
and yellow became green after adding 1 drop of 1M NaOH. On the other hand, in the buffered solution,
the acetate ions react with hydronium ions from the HCl that is added to the solution to neutralize the
added acid and there will suddenly be an excess of H+ ions from dissociation of the HCl. The buffers in
the solution will respond by binding these excess H+ ions to re-form the weak acid, using up the excess
H+ ions so that the pH can remain around the same value despite the addition of an acid. Also, hydroxide
ions from the strong base, NaOH, are neutralized by the acetic acid, forming water and acetate ion.
Therefore, there is no significant change in the solution as seen in the solution that it has no change in
color as it remain yellow after adding 50 drops of 1M HCl and 50 drops of 1M NaOH.
A. Make a tabular presentation of the data available from the virtual experiments.
B. Explain the observed data from the pH values of the given solutions.
C. Explain in 2 sentences how unbuffered solutions are compared to buffered solutions based on
the observed data and results.
Collate your answers and submit your group output in a word (.doc) file.
Methyl red indicator (pKa= 4.95) HCl (aq) (1.7 × 10-5 M) (250mL) HOAc/NaOAc (Buffer Solution=
Acetic acid and Sodium acetate)
pH (initial) 4.70 pH 4.70 pH
0.1 M of sodium hydroxide= Add Change in color ( red to pale No change in color (solution
10 drops each solution yellow) remain in red)
pH (final) 9.89 pH 4.73 pH
nOH- 0.1 M (0.0005L) 5.0 × 10-5 mol
=5.0 × 10-5 mol
∆ph 5.19 0.03
Buffer capacity 9.63 × 10-6 mol/pH unit 1.67 × 10-3 mol/pH unit
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