Titration Chemistry Lab Report Vitamin C

Experiment 1: Determining content of Vitamin C in vitamin tablets.
Aim: The aim of this experiment was to determine the content of vitamin C in
tablets using potassium iodate titration.
Experimental:
A vitamin C tablet (1.946g) was dissolved in water. The solution was filtered into a
250 cm3 volumetric flask and water was added up to the mark (Solution A).
Potassium iodate (1.345g) was dissolved in water and made up to the mark in a
250 cm3 volumetric flask to prepare a standard potassium iodate solution
(Solution B). Solution A (10.0 cm3), sulfuric acid (25 cm3, 2 M), potassium iodide
solution (20 cm3, 10%) and solution B (10 cm3) were added to a 500 cm3 conical
flask. Deionised water (135 cm3) was added into the flask to dilute the mixture to
200 cm3. Standard sodium thiosulfate solution (0.1014 M) was used to titrate the
mixture until a change in colour from brown to pale yellow was observed. Starch
solution (2 cm3) was added at this stage into the mixture, making its colour
change to a dark blue. The titration was then carried on until the disappearance of
the dark blue color indicated the end point of the reaction. The titration was
repeated until three concordant titres were obtained. The results were used to
calculate the concentration of the Vitamin C solution and determine the Vitamin C
content in the tablet. The mass of Vitamin C was 1.00958 g and the tablet
contained 51.88% of ascorbic acid.
Results:
S2O32- volume Titration 1 Titration 2 Titration 3 Titration 4

(cm3)
Final reading 10.60 20.97 10.76 21.09
Initial reading 0.14 10.60 0.42 10.76
Volume 10.46 10.37 10.34 10.33
delivered
Table 1 - Burette readings [S2O32-] = 0.1014M
Average titre = 10.35 cm3 of 0.1014M S2O32-
Moles of S2O32- used = 1.04949 x 10-3 mol
Moles of IO3- used= 2.514 x 10-4 mol
Moles of iodine generated = 7.542 x 10-4 mol
Moles of iodine reacting with vit. C = 2.2945 x 10-4 mol
[C6H8O6] in solution A = 0.022945 M

Vitamin C content in tablet = 1.00958 g
% of Vitamin C in tablet = 58.88 %
Discussion
Balanced equation for the oxidation of C6H8C6 by I3-
C6H8O6 + I3-  C6H6O6 + 3I- + 2H+
Concentration of KIO3 in Solution B

KIO3= 1.345g GFM = 214g
Moles of KIO3 = 1.345 : 214 = 0.006285 mol
Solution B volume = 250 cm3 = 0.25 dm3
[KIO3] = n/V = 0.006285 mol : 0.25 dm3 = 0.02514 M
Number of moles of iodate added into the mixture

Solution B added volume = 10 cm3 = 0.01 dm3
Solution B [KIO3] = 0.02514 M
n = c x V = 0.02514 mol/dm3 x 0.001 dm3 = 2.514 x 10 -4 mol
Number of moles of iodine (or tri-iodide) generated
Per 1 mole of iodate we have 3 moles of iodine
Moles of IO3- = 2.514 x 10-4 mol
Moles of I2 = 2.514 X 10-4 x 3 = 7.542 x 10-4 mol
Number of moles of thiosulphate used

[S2O32-] = 0.1014 M
Volume of S2O32- = 10.35 cm3 = 0.01035 dm3
n = c x V = 0.1014 mol/dm3 x 0.01035 dm3 = 1.04949 x 10-3 mol
Number of moles of iodine titrated

One mole of thiosulfate reacts with half mole of iodine
Moles of titrated iodine = 1.04949 x 10-3 : 2 = 5.24745 x 10-4 mol
Iodine moles that reacted with vitamin C

The difference between the moles of iodine generated and the moles of iodine
titrated by the thiosulfate corresponds to the moles that reacted with the ascorbic
acid:
7.542 x 10-4 mol - 5.24745 x 10-4 mol = 2.2945 x 10-4 mol
Ascorbic acid concentration in Solution A

One mole of iodine reacts with one mole of vitamin C
Vitamin C moles in 0.01 dm3 of solution A= 2.2945 x 10-4 mol
[C6H8O6] in sol. A = n/V = 2.2945 x 10-4 mol : 0.01 dm3 = 0.022945 M

Vitamin C content in tablet
Vitamin C moles in 250 cm3 of solution A:
n = c x V = 0.022945 mol/dm3 x 0.25 dm3= 5.73625 x 10-3 mol
Vitamin C GFM = 176 g
Vit. C mass in tablet = n x GFM = 5.73625 x 10-3 x 176 = 1.00958 g = 1009.58 mg
Vit. C % in tablet
Mass of tablet = 1.946 g
Mass of Vitamin C = 1.00958 g
% Vitamin C = 1.00958 g/ 1.946 g* 100 = 51.87975… = 51.88 % (to 2 d.p.)
The box states that the vitamin content of each tablet is 1000 mg. According to
the results obtained using the data originated during the experiment for the
calculations, there were 1009.58mg of ascorbic acid in the chosen tablet.
Therefore, even if the content appears to be higher than the stated amount in the
box, the difference is only minimal (being it of only 9.58 mg) compared to the
1000mg figure and it can be said that the results agree with the box statement.
Conclusion
The aim of the experiment was to calculate the vitamin C content in a tablet. The
results showed that the percentage of vitamin C in the tablet was 51.88%. The
tablet contained 1009.58 mg of vitamin C, a slightly higher mass than the one
stated in the box. The difference could derive from errors made during the
experiment. The burette readings could have been wrong, leading to a minimal
change in the results during different stages and, therefore, giving a slightly higher
number in the final calculation.

Titration Chemistry Lab Report Vitamin C

Uploaded by

Copyright:

Available Formats

Titration Chemistry Lab Report Vitamin C

Uploaded by

Document Information

Original Description:

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Titration Chemistry Lab Report Vitamin C

Uploaded by

Copyright:

Available Formats

Experiment 1: Determining content of Vitamin C in vitamin tablets.

S2O32- volume Titration 1 Titration 2 Titration 3 Titration 4

[C6H8O6] in solution A = 0.022945 M

C6H8O6 + I3-  C6H6O6 + 3I- + 2H+

Concentration of KIO3 in Solution B

Number of moles of iodate added into the mixture

Number of moles of thiosulphate used

Number of moles of iodine titrated

Iodine moles that reacted with vitamin C

Ascorbic acid concentration in Solution A

[C6H8O6] in sol. A = n/V = 2.2945 x 10-4 mol : 0.01 dm3 = 0.022945 M

You might also like