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    Pathik Desai Resume PDF

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    (1) This document is a resume for Pathik Rakesh Desai that outlines his education and professional experience in cell culture, protein analytics, molecular biology, and related laboratory techniques and equipment. (2) His most recent role is as a Senior Research Associate at Celgene where he performs tasks such as isolating immune cells, developing ELISA platforms, screening small molecules, generating cell lines, and analyzing samples using mass spectrometry.

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    Pathik Desai Resume PDF

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    (1) This document is a resume for Pathik Rakesh Desai that outlines his education and professional experience in cell culture, protein analytics, molecular biology, and related laboratory techniques and equipment. (2) His most recent role is as a Senior Research Associate at Celgene where he performs tasks such as isolating immune cells, developing ELISA platforms, screening small molecules, generating cell lines, and analyzing samples using mass spectrometry.

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    (1) This document is a resume for Pathik Rakesh Desai that outlines his education and professional experience in cell culture, protein analytics, molecular biology, and related laboratory techniques and equipment. (2) His most recent role is as a Senior Research Associate at Celgene where he performs tasks such as isolating immune cells, developing ELISA platforms, screening small molecules, generating cell lines, and analyzing samples using mass spectrometry.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    86 views1 page

    Pathik Desai Resume PDF

    Uploaded by

    pathik desai
    (1) This document is a resume for Pathik Rakesh Desai that outlines his education and professional experience in cell culture, protein analytics, molecular biology, and related laboratory techniques and equipment. (2) His most recent role is as a Senior Research Associate at Celgene where he performs tasks such as isolating immune cells, developing ELISA platforms, screening small molecules, generating cell lines, and analyzing samples using mass spectrometry.

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    Pathik Rakesh Desai

    26 Haskell St, Apt 2, Boston, MA-02134 |857.206.5525| [email protected]|https://www.linkedin.com/in/pathik-desai-/

    Core Competencies

    Cell Culture: Primary immune cells, Mammalian (THP1, Raji, RAMOS, H929, HEK-293, RAW 264.7, PANC-1), SF9, SF13

    Protein Analytics: Purification (Affinity, SEC, SCX, AEX, IMAC); UF/DF; MSD, Delfia, A280, HPLC, UPLC, Pierce 660.

    Molecular Biology: Reporter assays, SiRNA, Plasmid Purification and isolation; DNA & RNA extraction; RT-PCR; qPCR.

    Software: FlowJo, SAS JMP, Unicorn 7.0, EMPOWER, Chemstation, Sparklink, ELN-LIMS, Graphpad, Compass, Licor, Dotmatics.

    Equipment: AKTA Pure 25, AKTA Avant 25, AKTA Avant 150, AKTA Explorer, Agilent 1200, Waters Acquity, Bio-Rad Imager, LICOR,
    Charles River endotoxin reader, Alias Auto Sampler, Scilog TFF, Protein Simple WES and PEGGY.

    PROFESSIONAL EXPERIENCE

    Senior Research Associate, Biology, Celgene, Cambridge, MA January 2018 – Present


     Isolate PBMCs and monocytes for functional readouts using luciferase reporter assays.
     Receptor internalization analysis using FACs.
     Develop an ELISA platform for High Throughput screening of target proteins.
     Screen Small molecule PROTACS and generate Dose Response Curves in Mammalian cells.
     Transient and Stable transfection to generate disease relevant Cell lines.
     Perform Immunoprecipitations using polyclonal antibodies and Small molecule conjugated beads.
     Dose, Lyse, Desalt, TMT label and fractionate samples for fingerprinting using LC/MS.

    QC Associate I (Contract), Molecular Biology, Brammerbio, Cambridge, MA October 2017- January 2018
     Perform qPCR and ELISA to determine AAV2 and AAV6 Viral titers.
     Instrument Validation in accordance to cGMP regulations

    Research Co-op, Momenta Pharmaceuticals, Cambridge, MA January 2017- June 2017


     Develop HIC and Ion Exchange purification modalities for antibodies, Resulting in a single component pure compound.
     Charactize final probes using SEC, SDS-PAGE, and LAL testing.
     Conduct large scale Protein A, SCX, and Multicolumn purifications.
     Use DOE, Study Effect of Depth filtration on highly concentrated IVIG solutions.
     Evaluate multiple colorimetric protein kits for protein quantification.
     Perform Western Blots to detect presence of ubiquitinated proteins.

    Research Assistant, Tufts University School of Medicine, Boston, MA April 2016- December 2016
     Purify His tagged proteins.
     Treat Murine Macrophages and analyze lysates to study activation of various secondary messengers.
     Genotype mice using agarose gel electrophoresis.

    SEMINARS/ WORKSHOPS:
     Basic experimental setup for Gene-Editing with Crispr/Cas systems. -The Zhang Lab, Broad Institute
     Building biological systems, Genome synthesis and genome editing -Coursera

    EDUCATION

    M.S Pharmaceutical Sciences NORTHEASTERN UNIVERSITY June 2017

    Bachelor of Pharmacy M.E.T, University of Mumbai, India May 2015

    ACADEMIC PROJECT
    Project: Compare antioxidant activity of three plants of the family Zingiberaceae, Namely zingiber officinale, curcuma amada and
    curcuma longa.

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