COURSE PLAN

Department :
Biotechnology

Course Name & code :

Bioprocess Engineering & BIO 3102

Semester & branch :

V & B.TECH (BIOTECHNOLOGY)

Name of the faculty :

Dr. Subbalaxmi S

No of contact hours/week: L T P C
3 1 0 4

Course Outcomes (COs)

No. of
At the end of this course, the student should be able to: Contact Marks
Hours
CO1: Apply the kinetics of enzyme catalyzed reaction 10 25

CO2: Develop the medium for the industrial fermentation process 05 10

CO3: Estimate thermal death kinetic parameters of microorganism and design 9 15

the sterilization unit
CO4: Employ various concepts of transport phenomena in the design of 12 25
bioreactors
CO5: Illustrate microbial kinetics and determine kinetic parameters for growth 12 25
and product formation

Total 48 100

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 Assessment Plan

End Semester/
Components Assignments Sessional Tests
Make-up Examination
Duration 20 to 30 minutes 60 minutes 180 minutes
Weightage 20 % (4 X 5 marks) 30 % (2 X 15 Marks) 50 % (1 X 50 Marks)
Knowledge/ Recall; Understanding/
Understanding/ Comprehension;
Typology of Understanding/ Comprehension;
Application; Analysis; Synthesis;
Questions Comprehension; Application; Analysis;
Evaluation
Application Synthesis; Evaluation
Answer one randomly selected MCQ: 10 questions Answer all 5 full
question from the problem sheet (0.5 marks) questions of 10 marks
Pattern (Students can refer their class Short Answers: 5 each. Each question
notes) questions (2 marks) may have 2 to 3 parts
of 3/4/5/6/7 marks
4, 7, 10, and 13th week of
Schedule Calendared activity Calendared activity
academic calendar
Quiz 1 (L 1-12 & T 1-4 ) (CO1) Test 1 Comprehensive
(L a1-a2 & T b1-b2 ) examination covering
Topics Quiz 2 (L 13-20 & T 5-6 ) (CO2,3) (CO1-3) full syllabus. Students
Covered Quiz 3 (L 21-25 & T 7-8 ) (CO4) Test 2 are expected to answer
(L a3-a4 & T b3-b4 ) all questions (CO1-5)
Quiz 4 (L 26-33 & T 9-11 ) (CO5)
(CO3-5)

Lesson Plan

Course
L. No. Topics Outcome
Addressed
L0 Introduction to the course CO1-5

L1 Enzymes - Introduction, functions, classifications, Coenzyme and cofactor, enzyme CO1

specificity
L2 Michaelis-Menten Equation - Basics, assumptions CO1

T1 Tutorial CO1

L3 Michaelis-Menten Equation - derivation (Equilibrium approach & PSS approach) CO1

L4 Enzyme kinetic parameter estimation - various plots CO1

L5 Problems CO1

T2 Tutorial CO1

L6 Enzyme Inhibition - types, derivation CO1

L7 Enzyme Inhibition - derivation contd., CO1

L8 Problems CO1

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T3 Tutorial CO2

L9 History of Industrial fermentation, the basics of Industrial fermentation; CO2

Fermentation products, examples
L10 Medium requirements for fermentation processes- Carbon, nitrogen, minerals, CO2
vitamins
L11 Medium formulation for optimal growth and product formation; Design and usage of CO2
various commercial media for industrial fermentations
T4 Tutorial CO2

L12 Thermal death kinetics of microorganisms, heat -Sterilization of Liquid media CO3

L13 Del factor calculations, HTST concept CO3

L14 Batch and continuous sterilizers CO3

T5 Tutorial CO3

L15 Problems CO3

L16 Design of Sterilization Equipment CO3

L17 Immobilization methods - advantages, disadvantages CO4

T6 Tutorial CO3

L18 Immobilized enzyme/cell kinetics: Shell balance & effectiveness factor – zero order CO4

L19 Immobilized enzyme/cell kinetics: Shell balance & effectiveness factor – First order CO4

L20 Effect of geometry on effectiveness factor CO4

T7 Tutorial CO4

L21 Tutorial Oxygen transfer in submerged fermentation processes CO4

L22 OTR, OUR calculations CO4

L23 kLa estimations - various methods CO4

T8 Tutorial CO4

L24 role of aeration and agitation in oxygen transfer CO4

L25 Problems CO4

L26 Effect of environmental factors on microorganism growth. Cell Growth CO5

Measurements
T9 Tutorial CO4

L27 Effect of environmental factors on microorganism growth. Cell Growth CO5

Measurements
L28 Growth Cycle for Batch cultivation, Microbial cell kinetics CO5

L29 Simple unstructured kinetic models for microbial growth CO5

T10 Tutorial CO5

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 L30 Monod model; Growth of Filamentous Organisms. CO5

L31 Logistic model CO5

L32 Problems CO5

T11 Tutorial CO5

L33 Problems CO5

L34 Growth associated (primary) and non-growth associated (secondary) product CO5
formation kinetics;
L35 Leudeking Piret models; substrate and product inhibition on cell growth and product CO5
formation;
T12 Tutorial CO5

L36 Problems CO5

L/T Click or tap here to enter text.

References:

1. Michael L Shuler and Fikret Kargi. 2008. Bioprocess Engineering: Basic Concepts. Prentice-Hall of
India Pvt Ltd.
2. Pauline M. Doran. 1995. Bioprocess Engineering Principles. Pauline M. Doran. 1995.Academic
Press.
3. P. F. Stanbury, S. Hall, A. Whitaker. 2003. Principles of Fermentation Technology. 2nd Edition,
Elsevier Science Publishers.
4. Click or tap here to enter text.

5. Click or tap here to enter text.

6. Click or tap here to enter text.

7. Click or tap here to enter text.

Submitted by: DR SUBBALAXMI S

(Signature of the faculty)

(Page 4 of 5) MIT/GEN/F-01/R2
 Date: 24-07-2019

Approved by: DR M RAMANANDA BHAT

(Signature of HOD)

Date: Click or tap to enter a date.

FACULTY MEMBERS TEACHING THE COURSE (IF MULTIPLE SECTIONS EXIST):

FACULTY SECTION FACULTY SECTION

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