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    Lab 7 Column Chromatography

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    This document summarizes an experiment using column chromatography to separate ferrocene from acetylferrocene. Two solvents, petroleum ether and hexane ether, were used to develop chromatograms and calculate Rf values. With petroleum ether, the Rf values of ferrocene and one separated solution were identical, indicating they were the same compound. However, with hexane ether the Rf values did not provide clear results about what was separated. So hexane ether was not a good solvent for this separation based on the inability to relate the results to identify the compounds.

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    Lab 7 Column Chromatography

    Uploaded by

    jessyh2o
    2K views3 pages
    This document summarizes an experiment using column chromatography to separate ferrocene from acetylferrocene. Two solvents, petroleum ether and hexane ether, were used to develop chromatograms and calculate Rf values. With petroleum ether, the Rf values of ferrocene and one separated solution were identical, indicating they were the same compound. However, with hexane ether the Rf values did not provide clear results about what was separated. So hexane ether was not a good solvent for this separation based on the inability to relate the results to identify the compounds.

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    This document summarizes an experiment using column chromatography to separate ferrocene from acetylferrocene. Two solvents, petroleum ether and hexane ether, were used to develop chromatograms and calculate Rf values. With petroleum ether, the Rf values of ferrocene and one separated solution were identical, indicating they were the same compound. However, with hexane ether the Rf values did not provide clear results about what was separated. So hexane ether was not a good solvent for this separation based on the inability to relate the results to identify the compounds.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    2K views3 pages

    Lab 7 Column Chromatography

    Uploaded by

    jessyh2o
    This document summarizes an experiment using column chromatography to separate ferrocene from acetylferrocene. Two solvents, petroleum ether and hexane ether, were used to develop chromatograms and calculate Rf values. With petroleum ether, the Rf values of ferrocene and one separated solution were identical, indicating they were the same compound. However, with hexane ether the Rf values did not provide clear results about what was separated. So hexane ether was not a good solvent for this separation based on the inability to relate the results to identify the compounds.

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    of 3

    Organic 251:237B

    Experiment 7

    Column Chromatography

    Hajra Malik
    SCC 251:237B
    Experiment 7: Column Chromatography
    Date: May 13th, 2014
    Abstract
    In this experiment column chromatography was used to separate ferrocene from
    acetylferrocene. Column chromatography is a process in which we separate two
    compound mixtures and usually performed for small and large scale experiments. In the
    beginning we used TLC technique to select the solvent, and then we used dry packing
    method to perform the column chromatography procedure in which we used two
    solvents. As a result, two color patterns were observed orange and yellow. To separate
    yellow solution hexane solvent was added and to separate orange color pattern hexane
    ether solvent was added slowly to the column. After separating the compounds we
    spotted yellow, orange and ferrocene solution on the plate to see which solution was
    ferrocene with the help of solvent named Petroleum ether (non-polar). We concluded that
    Rf value for yellow solution was 0.86, for orange it was 0.76 and for ferrocene it was
    0.86. We can safely say that yellow solution was ferrocene based on the Rf values. On the
    other hand, we used hexane ether solvent for solutions to see the distance but
    unfortunately we couldnt find any related results based on Rf values.
    Data
    Table 1: Rf values using Petroleum Ether solvent
    Solutions

    Yellow

    Orange

    Ferrocene

    Rf = Distance travelled by
    compound/ distance travelled
    by solvent

    4.3/5.0=0.86

    3.8/5.0=0.76

    4.3/5.0=0.86

    Organic 251:237B

    Experiment 7

    Column Chromatography

    Fig. 1. A Chromatogram showing distance travelled by compounds and Petroleum Ether

    Table 2: Rf values using Hexane Ether solvent


    Solutions

    Yellow

    Orange

    Ferrocene

    Rf = Distance travelled by
    compound/ distance travelled
    by solvent

    0/5.0=0.0

    1.4/5.0=0.28

    3.5/5.0=0.7

    Fig. 2. A Chromatogram showing distance travelled by compounds and Hexane Ether

    Organic 251:237B

    Experiment 7

    Column Chromatography

    Discussion
    The procedure of column chromatography involved few important steps like
    choosing a stationary phase, choosing proper solvents, setting up apparatus, packing the
    column and identification of the solutions based on Rf values. We used two different
    solvents to measure the Rf values petroleum ether and hexane ether. With use of
    petroleum ether the Rf values were as followed; yellow: 0.86, orange: 0.76 and ferrocene:
    0.86. It is observed that ferrocene Rf value is similar to yellow solution so we can assume
    that yellow solution is ferrocene. On the other hand, with the use of hexane ether we
    found Rf values as followed: yellow: 0.0, orange: 1.4 and Ferrocene: 3.5. We couldnt see
    any similarity between the Rf values, hence, we cannot relate any data. As we knew,
    mixtures separate during stationary phase based on their polarity to the solvents. We
    expected that the mixtures would elute faster in petroleum ether and that is what we got
    in results. But we were not sure about the hexane ether solvent. As a result, we couldnt
    find the related data to show that if it was polar or non-polar. We could have improved the
    experiment technique by confirming the results of hexane ether solvent by performing it
    twice or spotted correctly on the plate. There are limitations to column chromatography
    technique. It gets complicated when we are separating large compounds and also two
    very similar compounds. There are highly chances of getting wrong results when it comes
    to similar compounds and thats probably why this technique is mostly used in industry
    where they can measure it automatically and accurately.

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