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    What Does 1% Horse DNA Mean Testing For DNA: Foreign

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    Sonu Somanath
    The document discusses DNA testing for food authentication and species identification. It explains that DNA can spread between unrelated species naturally or through human interventions like food processing. Testing food for foreign DNA is important for food safety, combating fraud, and meeting religious requirements. Methods like PCR, real-time PCR, and DNA sequencing can be used to detect, quantify, and identify DNA from different species in processed foods. Finding 1% or more horse DNA would require a product to be labeled as containing horse meat. DNA testing helps address various health, economic, social, and ethical implications of food contamination or adulteration.

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    What Does 1% Horse DNA Mean Testing For DNA: Foreign

    Uploaded by

    Sonu Somanath
    30 views24 pages
    The document discusses DNA testing for food authentication and species identification. It explains that DNA can spread between unrelated species naturally or through human interventions like food processing. Testing food for foreign DNA is important for food safety, combating fraud, and meeting religious requirements. Methods like PCR, real-time PCR, and DNA sequencing can be used to detect, quantify, and identify DNA from different species in processed foods. Finding 1% or more horse DNA would require a product to be labeled as containing horse meat. DNA testing helps address various health, economic, social, and ethical implications of food contamination or adulteration.

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    DIFSC

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    DIFSC_2013

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    The document discusses DNA testing for food authentication and species identification. It explains that DNA can spread between unrelated species naturally or through human interventions like food processing. Testing food for foreign DNA is important for food safety, combating fraud, and meeting religious requirements. Methods like PCR, real-time PCR, and DNA sequencing can be used to detect, quantify, and identify DNA from different species in processed foods. Finding 1% or more horse DNA would require a product to be labeled as containing horse meat. DNA testing helps address various health, economic, social, and ethical implications of food contamination or adulteration.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    30 views24 pages

    What Does 1% Horse DNA Mean Testing For DNA: Foreign

    Uploaded by

    Sonu Somanath
    The document discusses DNA testing for food authentication and species identification. It explains that DNA can spread between unrelated species naturally or through human interventions like food processing. Testing food for foreign DNA is important for food safety, combating fraud, and meeting religious requirements. Methods like PCR, real-time PCR, and DNA sequencing can be used to detect, quantify, and identify DNA from different species in processed foods. Finding 1% or more horse DNA would require a product to be labeled as containing horse meat. DNA testing helps address various health, economic, social, and ethical implications of food contamination or adulteration.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 24

    What does 1% Horse DNA Mean

    Testing for Foreign DNA

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    WHAT is FOREIGN DNA


    DNA is the genetic print of life.
    It is transferred from one generation to other carrying vital
    information of life.
    Has been widely used in Food testing procedures to detect
    pathogens, species identifications and determining GM
    content.
    DNA can spread to unrelated species by either
    naturally (antibiotic resistance among microorganisms) or
    human interventions (during food processing, creation of
    hybrids, introduction of gene to enhance quality and
    quantity of product)

    The latter is the foreign DNA


    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    FOREIGN DNA in FOOD


    Humans are continually exposed to DNA in
    Inhaled organisms,

    From a broad variety of food sources


    Via microorganisms normally present.

    Infectious agents entering the body


    Humans ingest 0.1 g to 1 g of DNA per day (Deorfler 2000).

    0.1% to 1% of dietary DNA is absorbed from the


    gastrointestinal tract (Nielsen et al; 2005a,2006)

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    FOREIGN DNA in FOOD


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION
    Food authentication and food safety are a

    growing concern.
    Food fraud is a universal problem to tackle.
    Mislabeling of Food
    Consumers are cheated by paying for a
    species that they are not actually getting.

    Fraud hampers conservation efforts (especially


    in sea food items).
    Enhances illegal trade activities

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    IMPLICATIONS OF FOREIGN DNA


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION

    Food adulteration or contamination can lead to


    Physiological Effect
    Can have health impacts when species are riddled
    with contaminants.
    Significant public health issues will arise
    Toxic to sensitive consumers.
    Allergens can get introduced into our health system.

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    IMPLICATIONS OF FOREIGN DNA


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION

    Food adulteration or contamination can lead to


    Economical Effects
    Detrimental to business some companies get
    benefited.
    The horsemeat scandal in Europe served as a strong
    reminder of how damaging unreliable suppliers can
    be to corporate brands.
    It robs hard-earned profits from honest suppliers and
    businesses through unfair competition.

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    IMPLICATIONS OF FOREIGN DNA


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION
    LETS TRY

    Escolar

    Nile Perch

    Mako Shark

    Rock Fish

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    IMPLICATIONS OF FOREIGN DNA


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION

    Food adulteration or contamination can lead to


    Social and Ethical effects
    the impacts of fraud can be social and ethical
    concerns.
    Widespread public fear
    Loss of public confidence in safety of food
    Effectiveness of authorities is questioned?

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    IMPLICATIONS OF FOREIGN DNA


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION

    Food adulteration or contamination can lead to


    Religious
    Presence of certain contaminants is not accepted by
    religious beliefs.
    Pork has zero tolerance in the region.
    Certain Food items are not eaten in other parts of the
    world.

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    PRESENCE OF HORSE DNA - 1%


    Indicates that the food is contaminated with horse meat
    First reported by FSAI 15th January
    Followed by FSA UK performing screening tests and identifying

    products that had presence of horse and pork meat.


    Subsequently other reports emerged and a legal framework was
    designed.
    1% is the minimum threshold level for DNA or protein resulting
    from the DNA present in the food product above which labeling
    is mandatory

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    PRESENCE OF HORSE DNA - 1%

    The new UK meat testing regime agreed by the Food Standards Agency,
    retailers and processors will allow for a 1% tolerance level for the DNA of other
    species in meat products.

    At the meeting, the industry also agreed to share its own meat DNA test results
    with the FSA.

    FSA is carrying out its own food authenticity programme on processed meat
    lines.

    It is understood the 1% level will apply to both the industrys own tests and the
    FSA authenticity programme.

    The FSA and industry are confident that labs can reliably test to 1% and that
    levels of DNA at sub 1% are unlikely to indicate deliberate contamination,

    We will be looking at this issue further and engaging directly with consumers as
    we move forward.

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    TESTING FOREIGN DNA


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION

    DNA testing has the answer


    Accurately detect the presence or
    absence.
    Quantify to meet the regulations of

    allowable limits.
    Identify different species present in

    processed food.

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    TESTING FOREIGN DNA


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION

    Polymerase Chain Reaction (PCR)


    REAL TIME PCR

    DNA SEQUENCING

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    TESTING FOREIGN DNA - PCR


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION
    Denaturation

    ds DNA denatured
    by heating >94oC

    Extension

    Annealing

    1 PCR
    cycle

    Taq polymerase
    extends target
    sequences

    Oligo primers bind


    to target sequences

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    TESTING FOREIGN DNA Real Time PCR


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION
    3
    3
    5
    Forward Primer

    R
    5

    Q
    Reverse Primer
    3
    5
    3

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    TESTING FOREIGN DNA - PCR


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION
    DNA based testing for Species Identification

    2013

    600 + total Samples


    4000+ total Samples

    2012

    2008

    2005

    Food Authentication and Species


    Identification

    Water and Food-borne Pathogen

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    TESTING FOREIGN DNA - PCR


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION
    DNA based testing for Horse Species Identification

    2013

    200+ total Samples

    1 Positive

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    TESTING FOREIGN DNA DNA SEQUENCING


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION

    DNA sequencing Procedures


    1.
    2.
    3.
    4.
    5.
    6.

    7.

    DNA Extraction & Quantitation


    DNA Amplification
    PCR product purification
    Cycle sequencing
    Cycle sequencing product
    clean up
    Amplified DNA product
    Separation using Capillary
    Electrophoresis
    Sequence Analysis

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    TESTING FOREIGN DNA DNA SEQUENCING


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION
    DNA Sequencing based Identifications

    2013

    300+ total Samples


    4000+ total Samples

    2012

    2008

    2005

    Food Authentication and Species


    Identification

    Water and Food-borne Pathogen

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    TESTING FOREIGN DNA


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION

    DNA Barcoding CO1 Sequencing BOLD


    Unique sequence that assists in Species identification
    Developed by Canadian Centre for DNA Barcoding

    NCBI Genebank Database

    Amplified and sequenced Species specific DNA sequences


    can be matched and identified from the database.

    FDA sea food Authentication

    Recently USFDA has developed protocol for Seafood


    identification based on mitochondrial CO1 sequences.

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    TESTING FOREIGN DNA


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION

    Species Identification by BOLD system

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    TESTING FOREIGN DNA


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION

    Species Identification by NCBI system


    www.ncbi.nih.gov/blast
    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    TESTING FOREIGN DNA


    FOOD AUTHENTICATION and SPECIES IDENTIFICATION
    DNA testing can solve many a problems for Food
    authentication.

    Choose the right technique depending on the


    complexity of the sample and also the results required.
    Establish a Food screening program for all stakeholders

    in Food industry and ensure integrity of the product and


    consumer interest.
    Dubai Municipality Proactive Approach
    One of the few Municipalities in the world that supports and

    propagates DNA based testing services.

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

    THANK YOU ALL

    ISO 17025 Accredited Laboratory


    LB-62-Test

    306, Riqa Al Buteen Plaza


    Al Maktoum Road
    Dubai
    Tel: 00971 4 2270004
    Fax: 00971 4 2270005

    DIFSC 2013 | Advance Biotechnology Centre, Dubai | Dr. Sanjeet Mishra

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