Embroyonic Stem Cells.

III. What are embryonic stem cells?
A. What stages of early embryonic development are important for generating embryonic
stem cells?
Embryonic stem cells, as their name suggests, are derived from embryos. Specifically,
embryonic stem cells are derived from embryos that develop from eggs that have been
fertilized in vitro—in an in vitro fertilization clinic—and then donated for research
purposes with informed consent of the donors. They are not derived from eggs fertilized
in a woman's body. The embryos from which human embryonic stem cells are
derived are typically four or five days old and are a hollow microscopic ball of cells called
the blastocyst. The blastocyst includes three structures: the trophoblast, which is the
layer of cells that surrounds the blastocyst; the blastocoel, which is the hollow cavity
inside the blastocyst; and the inner cell mass, which is a group of approximately 30
cells at one end of the blastocoel.
B. How are embryonic stem cells grown in the laboratory?

Growing cells in the laboratory is known as cell culture. Human embryonic stem cells
are isolated by transferring the inner cell mass into a plastic laboratory culture dish that
contains a nutrient broth known as culture medium. The cells divide and spread over
the surface of the dish. The inner surface of the culture dish is typically coated with
mouse embryonic skin cells that have been treated so they will not divide. This coating
layer of cells is called a feeder layer. The reason for having the mouse cells in the
bottom of the culture dish is to give the inner cell mass cells a sticky surface to which
they can attach. Also, the feeder cells release nutrients into the culture medium.
Recently, scientists have begun to devise ways of growing embryonic stem cells without
the mouse feeder cells. This is a significant scientific advancement because of the risk
that viruses or other macromolecules in the mouse cells may be transmitted to the
human cells.
Over the course of several days, the cells of the inner cell mass proliferate and begin to
crowd the culture dish. When this occurs, they are removed gently and plated into
several fresh culture dishes. The process of replating the cells is repeated many times
and for many months, and is called subculturing. Each cycle of subculturing the cells is
referred to as a passage. After six months or more, the original 30 cells of the inner cell
mass yield millions of embryonic stem cells. Embryonic stem cells that have proliferated
in cell culture for six or more months without differentiating, are pluripotent, and
appear genetically normal are referred to as an embryonic stem cell line.
Once cell lines are established, or even before that stage, batches of them can be frozen
and shipped to other laboratories for further culture and experimentation.
C. What laboratory tests are used to identify embryonic stem cells?

At various points during the process of generating embryonic stem cell lines, scientists
test the cells to see whether they exhibit the fundamental properties that make them
embryonic stem cells. This process is called characterization.
As yet, scientists who study human embryonic stem cells have not agreed on a standard
battery of tests that measure the cells' fundamental properties. Also, scientists
acknowledge that many of the tests they do use may not be good indicators of the cells'
most important biological properties and functions. Nevertheless, laboratories that grow
human embryonic stem cell lines use several kinds of tests. These tests include:
• growing and subculturing the stem cells for many months. This ensures that the
cells are capable of long-term self-renewal. Scientists inspect the cultures through
a microscope to see that the cells look healthy and remain undifferentiated.
• using specific techniques to determine the presence of surface markers that are
found only on undifferentiated cells. Another important test is for the presence of
a protein called Oct-4, which undifferentiated cells typically make. Oct-4 is a
transcription factor, meaning that it helps turn genes on and off at the right time,
which is an important part of the processes of cell differentiation and embryonic
development.
• examining the chromosomes under a microscope. This is a method to assess
whether the chromosomes are damaged or if the number of chromosomes has
changed. It does not detect genetic mutations in the cells.
• determining whether the cells can be subcultured after freezing, thawing, and
replating.
• testing whether the human embryonic stem cells are pluripotent by 1) allowing
the cells to differentiate spontaneously in cell culture; 2) manipulating the cells so
they will differentiate to form specific cell types; or 3) injecting the cells into an
immunosuppressed mouse to test for the formation of a benign tumor called
ateratoma. Teratomas typically contain a mixture of many differentiated or partly
differentiated cell types—an indication that the embryonic stem cells are capable
of differentiating into multiple cell types.
V. What are the similarities and differences between embryonic and adult stem
cells?
Human embryonic and adult stem cells each have advantages and disadvantages
regarding potential use for cell-based regenerative therapies. Of course, adult and
embryonic stem cells differ in the number and type of differentiated cells types they can
become. Embryonic stem cells can become all cell types of the body because they
are pluripotent. Adult stem cells are generally limited to differentiating into different cell
types of their tissue of origin. However, some evidence suggests that adult stem
cell plasticity may exist, increasing the number of cell types a given adult stem cell can
become.
Large numbers of embryonic stem cells can be relatively easily grown in culture, while
adult stem cells are rare in mature tissues and methods for expanding their numbers
in cell culture have not yet been worked out. This is an important distinction, as large
numbers of cells are needed for stem cell replacement therapies.
A potential advantage of using stem cells from an adult is that the patient's own cells
could be expanded in culture and then reintroduced into the patient. The use of the
patient's own adult stem cells would mean that the cells would not be rejected by the
immune system. This represents a significant advantage as immune rejection is a difficult
problem that can only be circumvented with immunosuppressive drugs.
Embryonic stem cells from a donor introduced into a patient could cause transplant
rejection. However, whether the recipient would reject donor embryonic stem cells has
not been determined in human experiments.

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III. What are embryonic stem cells?

B. How are embryonic stem cells grown in the laboratory?

C. What laboratory tests are used to identify embryonic stem cells?

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