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    sciFLEX QuickStart

    Uploaded by

    Jessie A. Cruz
    This document provides instructions for starting up and optimizing the sciFLEXARRAYER liquid handling system. It contains a pre-power up checklist to ensure proper setup. The steps include powering on the system components, selecting the desired liquid channels, running a morning wash procedure, adjusting the nozzle position using offset values, entering nozzle parameters like voltage and pulse obtained during drop optimization, and fine tuning the parameters until the drop distance is within specifications. The goal is to have the nozzle dispense a stable drop with the correct shape and position for array spotting.

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    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd

    sciFLEX QuickStart

    Uploaded by

    Jessie A. Cruz
    192 views1 page
    This document provides instructions for starting up and optimizing the sciFLEXARRAYER liquid handling system. It contains a pre-power up checklist to ensure proper setup. The steps include powering on the system components, selecting the desired liquid channels, running a morning wash procedure, adjusting the nozzle position using offset values, entering nozzle parameters like voltage and pulse obtained during drop optimization, and fine tuning the parameters until the drop distance is within specifications. The goal is to have the nozzle dispense a stable drop with the correct shape and position for array spotting.

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    sciFLEX_QuickStart

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    This document provides instructions for starting up and optimizing the sciFLEXARRAYER liquid handling system. It contains a pre-power up checklist to ensure proper setup. The steps include powering on the system components, selecting the desired liquid channels, running a morning wash procedure, adjusting the nozzle position using offset values, entering nozzle parameters like voltage and pulse obtained during drop optimization, and fine tuning the parameters until the drop distance is within specifications. The goal is to have the nozzle dispense a stable drop with the correct shape and position for array spotting.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    192 views1 page

    sciFLEX QuickStart

    Uploaded by

    Jessie A. Cruz
    This document provides instructions for starting up and optimizing the sciFLEXARRAYER liquid handling system. It contains a pre-power up checklist to ensure proper setup. The steps include powering on the system components, selecting the desired liquid channels, running a morning wash procedure, adjusting the nozzle position using offset values, entering nozzle parameters like voltage and pulse obtained during drop optimization, and fine tuning the parameters until the drop distance is within specifications. The goal is to have the nozzle dispense a stable drop with the correct shape and position for array spotting.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    sciFLEX Quick Start

    PRE-POWER UP CHECKLIST
    Wash bottle filled....................................................................................... CHECK
    Waste bottle empty .................................................................................. CHECK
    System liquid bottle filled to appropriate level.................................... CHECK
    • Use filtered (0,22µm) freshly degased millipore water
    • The system liquid surface and the nozzle tip (when in front of the camera station)
    !
    must be on the same level.

    sciFLEXARRAYER stage is clear and free of obstacles ....................... CHECK


    All cover hood doors ............................................................................. CLOSED
    • The doors must be closed to activate the magnetic door sensors.

    Emergency stop switches ....................................................................... ARMED


    • The emergency stop switches must be pulled out (one in the S3; two in S5/S11).

    POWERING UP
    Multi-outlet power strip switch ........................................................ SWITCH ON
    "Clunk"-sound of the spotter ................................................................... CHECK
    • After ~20 seconds of powering the axes (S5/S11 only), a distinct sound is audible.

    Computer ......................................................................................... SWITCH ON


    sciFLEX Software ................................................................. START INIT AXES
    Software started without error messages ............................................. CHECK

    FLUIDIC SETUP (1)

    Desired liquid channel(s) .......................................................................... SELECT


    • In the sciFLEX-Software click the tab Nozzle Setup. (2)
    • Click the green buttons corresponding to the desired channel to activate it (1).

    Task "MorningWashProcedure" .......................................................... ACTIVATE


    • Click the tab Nozzle Setup and then Do Task (2).

    • Select MorningWashProcedure and click DO to start.


    • A pop-up window will guide you through the operation (duration 20 mins).

    DROP OPTIMIZATION (3)

    • In the SNAP WINDOW click: Image > show center to obtain the centered green cross.
    • For better nozzle visibility activate the strobe LED by setting a pulse of 1µs and
    select Continuous Dispensing and click START (image will go bright).

    Nozzle position offset values ...................................................................ADJUST (4)


    • In the Nozzle Offset tab, adjust the nozzle position via the arrow buttons
    until the nozzle is positioned as shown in (3).
    • Click START again to turn off the piezo (Image will go dark).

    Nozzle parameter........................................................................................ ENTER


    • Set the parameters for Voltage and Pulse you obtained for your nozzle, into
    the according fields (4).

    • Save the parameters by clicking the Set Nozzle Parameter button.


    • Select Continuous Dispensing in the dispense mode window and click START.
    (5)
    A drop should be visible now.
    • Fine tune the parameters until the drop distance is approx. 450 ± 25 µm (5).
    450 ± 25µm
    OK Not OK
    Possible drop shapes:
    instable shape satellite position
    v1.20 - 03.04.2009

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