B.Sc. Biotechnology Semester III BBT 312: Biochemistry Ii Total 45 L

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B.Sc.

Biotechnology SemesterIII BBT 312: Biochemistry II


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Total 45 L
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Bioenergetics Anabolism and catabolism. Energy transformations obey laws of thermodynamics, enthalpy, entropy and free energy changes, oxidation and reduction, redox potential, high energy bond/compounds. Enzymes Introduction, specificity of enzymes, brief classification and nomenclature, factors affecting enzyme activity, mechanism of enzyme actions , enzyme kinetics Michaelis Menten rate equation, regulatory enzymes, enzyme inhibition allosteric, feedback, competitive and noncompetitive inhibition. Carbohydrate metabolism Glycogenesis, gluconeogenesis, glycogenolysis, glycolytic pathway, hormonal regulation and energetics. Entry of pyruvate in citric acid cycle. Glyoxylate cycle. Citric acid cycle Citric acid cycle, enzymes and regulation, energetics. Role of mutant in citric acid production. Amphibolic nature of citric acid cycle. Electron transfer chain Electron transfer chain, enzymes, pathway and regulation and oxidative phosphorylation in mitochondria. Lipid metabolism Absorption, transport, storage and oxidation of fatty acids (saturated with odd and even no. of carbon atoms), oxidation of unsaturated fatty acids, regulation of fatty acid oxidation. Nitrogen metabolism Overview of nitrogen metabolism, digestion of proteins to peptides and amino acids, essential amino acids, transamination of amino acids, nitrogen excretion and urea cycle. Biotech applications of peptides. Photosynthesis Photosynthetic pigments, light reaction (absorption of light, ETC and cyclic and noncyclic photophophorylation) and dark reactions, photorespiration, C4 and CAM metabolism. References 1. Biochemistry, Berg, J.M., Tymoczko, J. L. and Stryer, L. W.H. Freeman and Co., New York, USA (2003). 2. Principles of Biochemistry, Lehninger, A., Nelson, D.L and Cox, M. M., W.H. Freeman and Co., New York, USA (2008). 3. Biochemistry, Satyanarayan, U., Books and Allied (P) Ltd., Kolkata, India (2008).

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