Xpress Biologics now part of Sartorius’ Post

#Article now available! Precision in production: optimizing monitoring and quality control for high-value #plasmids Variable pathlength technology, also known as slope spectroscopy, addresses issues of around ensuring regulatory compliance, and lack of industry standardization by adjusting pathlengths to maintain a constant concentration and eliminate the need for dilutions. In this article, the SoloVPE® system used to determine plasmid #DNApurity ratios will be explored, along with other #variablepathlengthtechnology applications across various stages of #genetherapy, including fermentation, downstream processing, chromatography, #mRNApurity measurements, and AAV titer analysis. Read at your convenience below:

#Article now available! Precision in production: optimizing monitoring and quality control for high-value #plasmids Variable pathlength technology, also known as slope spectroscopy, addresses issues of around ensuring regulatory compliance, and lack of industry standardization by adjusting pathlengths to maintain a constant concentration and eliminate the need for dilutions. In this article, the SoloVPE® system used to determine plasmid #DNApurity ratios will be explored, along with other #variablepathlengthtechnology applications across various stages of #genetherapy, including fermentation, downstream processing, chromatography, #mRNApurity measurements, and AAV titer analysis. Read at your convenience below:

#Article now available! Accelerate #genetherapy downstream process development using scalable pre-packed #chromatographycolumns Single-use products such as pre-packed chromatography columns can be utilized to meet the growing demands for AAV-based #genetherapies due to their ability to reduce risks in manufacturing processes, save costs, and enhance facility efficiency. Here, how pre-packed chromatography columns are applied for the development and scale-up of #AAVpurification workflows is discussed. Read below:

Optimizing cell culture media is a key success factor necessary for high yield / high titer production processes. When MilliporeSigma started optimizing media across several CHOZN® cell lines, they immediately turned to Culture Biosciences’ services with cloud-connected bioreactors to optimize their bioprocess in real-time. Download this white paper to learn how MilliporeSigma: - Validated scale-up robustness - Generated significant increase in yield / titer for multiple CHOZN® cell lines - Validated results at scale #processdevelopment #processoptimization #CHO #mAb https://lnkd.in/eaDEmi2r

We understand that typical #ATMP bioprocessing methods are highly variable and challenging to characterise, and as one of our Challenge Themes we are committed to improving understanding of this process. We invite partners to our Process Analytical Technologies lab, a specialist facility that is dedicated to enable the rapid generation of detailed and automated process understanding, where we can work together to improve our understanding, improve outcomes and satisfy regulatory authorities. Learn more about our Challenge Themes: https://buff.ly/44lGFOu #CellTherapy #GeneTherapy #ChallengeThemes #AdvancedTherapies

Beyond Viability: Advancing CHO Cell Culture Process Strategies to Modulate Host Cell Protein Levels While purification strategies usually offer substantial HCP clearance, certain problematic HCPs, particularly lipases, pose significant challenges. This study investigates the accumulation of various problematic HCPs in CHO cell culture using transcriptomics, revealing correlations between cell culture parameters and HCP activity. Contrary to conventional assumptions, viability alone does not reliably predict HCP levels, with factors such as clone selection, host cell line choice, and media feed type significantly influencing HCP accumulation. Leveraging transcriptomics-based approaches, we demonstrate the potential of upstream process control strategies to mitigate HCP presence and improve biologic product quality. Our findings underscore the importance of considering diverse cell culture parameters in bioprocess optimization to ensure product stability and quality. https://lnkd.in/g27Z3pc3 #aspenalert #biotech #bioprocess

Interested in cell culture optimization. Learn more by downloading our whitepaper highlighting the Culture Biosciences collaboration with MilliporeSigma. #processoptimization #bioreactors #cloudconnectivity #bioprocess

A more detailed story behind our TNIK paper in Nature Biotechnology. Thank you, my dear friends, who took part in this. https://lnkd.in/d33PhHE4

🔬 Streamline Your Assays with Automated In Situ Supernatant Sampling! In drug discovery, compound screening, and organoid research, analyzing secreted factors from 3D cell models is crucial. But this step is often manual, labor-intensive, and taking plates out of the incubator can impact results. 😰    ✨ Enter the Pu·MA System – a breakthrough in assay automation. 🤩 With in situ supernatant sampling, you can easily collect conditioned media at multiple time points without disturbing your spheroids. This automation enhances data quality and provides deeper insights into the phenotypic and physiological state of your models. ➡ Check out this application: https://lnkd.in/gizcqD_i #OrganoidResearch #DrugDiscovery #3DCellModels #InnovationInResearch #PuMASystem #AssayAutomation #LabTech 

Struggling to identify irregularities in your cell cultures? We know how important a healthy cell culture is for successful outcomes in research and production processes. One key feature by using our cell counter NucleoCounter® NC-202™ is the DebrisIndex™ monitor tool, which provides insights into the amount of debris present in your sample relative to cell concentration, allowing researchers and manufacturers to ensure the quality and viability of the cells in your cell culture work. Let’s work together to accelerate the development and production of life-saving medications using innovative biotechnology. Learn more about the DebrisIndex™ here: https://okt.to/9m7uP5