TargetMol’s Post

https://lnkd.in/gaDyyqfu   Article title: Synthesis, characterization, anti-cancer evaluation, and DNA-binding study of new bay-substituted perylene derivatives   Author(S): Arwa Abourajab, S Melika Mostafanejad, Meltem Dinleyici, Basma Al-Khateeb, Imge Kunter, Sukru Tuzmen and Huriye Icil*   Journal: Journal of Biology and Medicine   Journal ISSN: 2688-8408 Abstract: Two new perylene derivatives 1,7-di(3,5-diamino-pyrimidoxyl) perylene-3,4,9,10- tetracarboxylic acid bisanhydride (4) and 1,7-di(2-[3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-1,3-thiazol-3-ium-5-yl]) ethoxyperylene-3,4,9,10-tetracarboxylic bisanhydride (6) have been synthesized. We aimed to study their interactions with G-quadruplex (G4) structures as potent G4 ligands and telomerase inhibitors. We used a PCR-amplified guanine-rich region from the human beta-globin gene, oligonucleotide from human telomeres (a-coreTT), an oncogene (c-kit), and SK-HEP-1 adenocarcinoma cells to characterize those compounds’ binding and stabilizing abilities to G4 structures and anti-cancer potential. All results obtained through UV-visible and fluorescence spectroscopies, agarose gel electrophoresis, and MTT assay on SK-HEP-1 adenocarcinoma cells were in good agreement. Compounds 4 and 6 are promising DNA-binding and cytotoxic compounds with a relatively antiproliferative effect on the selected tumour. In all studies, the formal positive charge carrier, compound 6, showed higher activity in terms of anti-cancer effects. These results may help elucidate the feasibility of the perylene derivatives as future chemo-therapeutic agents. #Perylenebisanhydride #Guanine #PCR #Oligonucleotide #Electrophoresis #MTTassay   #Biochemistry #Biotechnology #Bioinformatics #Cardiology #CellBiology #Peertechz #PeertechzPublications #FoodAndNutrition #FreeRadicalResearch #InfectiousAndCommunicableDiseases #Genetics #Gerontology #Microbiology #Hepatology #Gastroenterology #Toxicology #MedicinalChemistry #Nanotechnology #Oncology #Pharmacology #PlantBiology #Pathology #Physiology #RadiationBiology #Nephrology #StemCellResearch #VascularBiology

Effect of the Nitrogen Source on the Synthesis of Secondary Metabolites by Suspension Culture of Medicinal Asparagus Asparagus officinalis L. https://lnkd.in/ene9YR5u #Medicinalasparagus #suspensionculture #biologicallyactivesubstances #phenols #steroidalsaponins #nitrogensource #asparagus #nitrogen Abstract Medicinal asparagus contains unique compounds that can stop the development of cancerous tumors and even destroy cancer cells. However, there is still insufficient information about the features of the biosynthesis of these compounds and the conditions for their production. In this paper, the influence of an inorganic nitrogen source on the growth of a suspended culture of medicinal asparagus, the accumulation of phenolic compounds in the culture and culture liquid, and the synthesis of saponins is studied. A callus and suspension culture of medicinal asparagus Asparagus officinalis L. were obtained under in vitro conditions. For further research, concentrations of growth regulators 1 mg/L kinetin and 1.5 mg/L 2,4-D were selected. The fastest growth of the suspension culture was established on the medium with a concentration of potassium nitrate of 1900 mg/L and ammonium sulfate of 250 mg/L. Individual explants demonstrated spontaneous differentiation of callus tissue that affected the increase in callus mass and the ratio of the concentration of exophenols to the total mass of the explant. The highest concentrations of endo- and exophenols were obtained on a medium without ammonium nitrogen sources. A positive effect of the medium with a concentration of ammonium sulfate of 500 mg/L on the synthesis of the main steroidal saponins of Asparagus officinalis L. was determined. As a result of the study, a modified MS medium was developed that promotes the accumulation of steroid saponins in a suspension culture of medicinal asparagus. Keywords:  Medicinal asparagus suspension culture biologically active substances phenols steroidal saponins nitrogen source asparagus nitrogen

I'm pleased to announce that my recent research paper, titled "Differential Tissue Abundance of Membrane-Bound Drug-Metabolizing Enzymes and Transporter Proteins by Global Proteomics (https://lnkd.in/gZ8svtKv)," has been published in the Drug Metabolism and Disposition journal. We quantified the abundance and compositions of drug-metabolizing enzymes and transporters (DMETs) in pooled human liver, intestine, and kidney microsomes using an optimized novel sequence coverage-informed total protein approach (SC-TPA). Additionally, we identified high variability in the levels of intestinal DMEs (e.g., CYP3A4, CYP3A5, UGT2B17, CES2, and MGST2). The data generated regarding DMET abundance in the human liver, intestine, and kidney hold significant potential for informing the development of physiologically based pharmacokinetic (PBPK) models, particularly in explaining the role of enzymes and transporters in bioavailability and tissue-specific toxicity. Thanks to co-authors, Deepak Ahire, PhD, Dmitri Davydov, and my mentor Bhagwat Prasad, for their critical inputs. #https://lnkd.in/g2EzYH7P #https://lnkd.in/g8uVXkiu #ASPET

Please check out our newest paper that has been published in Molecular Therapy - Nucleic Acids. 😊

*Liver Function Tests in a nutshell *When to test for albumin? and when to test for acute liver biomarkers? *Liver conjugating enzymes can be assessed by the estimation of bilirubin. When direct bilirubin is elevated? and when the indirect form is?? *Compare ALT to AST? *Alkaline phosphatase (ALP) can be estimated hand in hand with Gamma-GT to confirm a gall bladder condition. *Tags: #biochemistry #laboratory #physician #liver #lab #pathology https://lnkd.in/dXEPSq-F

🔬Citation Break!📖 This citation investigates the potential use of compounds for their anti-aggregation effects on Synuclein and Tau proteins. rPeptide's Alpha-Synuclein (cat#S-1001) and Tau-441 (cat# T-1001) were used successfully in various biophysical experiments including thioflavin assays, PICUP assays, and TEM. Our monomeric products can be used with a range of applications such as these. We also offer labeled products, mutants, and preformed fibrils of both Synuclein and Tau! Paper: https://t.ly/HEsCJ Alpha-Synuclein: https://t.ly/AeISq Tau-441: https://t.ly/-hdBI #Research #Development #Experiment #Recombinant #Protiens #Peptides #rPeptide

🔬🧪 I just came across an incredibly fascinating article that I can't help but share with all of you! It's titled "Antibody–peptide conjugates deliver covalent inhibitors blocking oncogenic cathepsins" and it's published in the esteemed Nature Chemical Biology. As someone who has a deep love for #peptides, this comprehensive study captivated me from start to finish. It delves into the utility of peptides in antibody-peptide conjugate settings and provides a thorough understanding of the biochemical, cellular targeting, and biophysical aspects. 🧬 What makes this study even more intriguing are the two patents associated with it! And for those who love diving into the details, there's PDB data available - a real cherry on the cake! 🍒 The authors have used a variety of approaches including cell sorting, HRMS, western blotting, XRD, and more to paint a complete picture of their research. The depth and breadth of their investigation is truly commendable. I highly recommend this article to all peptide enthusiasts out there. It's not just a read, it's an experience! 📚 Please note: I am not associated with this paper or its authors in any way. I'm just a fan of great science and love to share interesting reads with my network. Happy reading and have a great weekend! 🎉 🔗 https://lnkd.in/gRNK-Mcg #Peptide #PeptideScience #NatureChemicalBiology #ScienceReading #WeekendReading #KnowledgeSharing

𝐂𝐨𝐦𝐩𝐫𝐞𝐡𝐞𝐧𝐬𝐢𝐯𝐞 𝐏𝐃𝐅 𝐆𝐮𝐢𝐝𝐞 𝐭𝐨 #Organoids [𝐏𝐃𝐅 𝐆𝐮𝐢𝐝𝐞] 📚 𝐆𝐞𝐭 𝐏𝐃𝐅 𝐒𝐚𝐦𝐩𝐥𝐞 𝐂𝐨𝐩𝐲: @ https://shorturl.at/jKm3s The organoids market size is projected to reach US$ 15.01 billion by 2031 from US$ 3.03 billion in 2023. The market is expected to register a #CAGR of 22.3% in 2023–2031. The combination of organoids and organ-on-a-chip is likely to act as one of the key #trends in the organoids market in the future. Organoids Market Analysis Factors contributing to the organoids market growth include the increasing demand for #tumor modeling and #biobanking, and a surging adoption of personalized drugs. However, the dearth of skilled professionals and stringent safety regulations hamper the #market growth. #organoids #stemcells #science #research #biology #alphamedpress #stemcellsportal #regenerativemedicine #scicomm #microscopy #cells #developmentalbiology #stemcellsjournals #instascience #cellculture #review #cellbiology #sciart #phd #organoid #escsipscs #unibonn #stem #thestemcellsjournal #technology #stemcellsjournal #cancer #liver #recentlypublished #immunology

Checkout the 3D Organoid Cell Viability Assay Kit by Assay Genie — available now at FroggaBio! This sensitive, one-step kit quantifies live cells in 2D and 3D models for accurate proliferation and cytotoxicity studies. 🐸🔬💡 CONTACT US TO LEARN MORE: https://hubs.li/Q02D1dwS0 The Assay Genie 3D Organoid Cell Viability Assay Kit offers a highly sensitive and straightforward method to quantify live cells in both 2D and 3D cultures. Validated in various models, including high-throughput 384-well formats, this kit uses WST-8 Tetrazolium salt for colorimetric detection. It boasts superior sensitivity and lower cytotoxicity compared to other Tetrazolium reagents, making it ideal for long-term experiments. The kit is easy to use with no pre-treatments required and delivers accurate results, enhancing your cell viability and cytotoxicity studies. #CellViability #AssayGenie #3DOrganoid #CellCulture #Cytotoxicity #ProliferationStudies Assay Genie #HighThroughput #WST8 #TetrazoliumAssay #ScientificResearch #BioTech #CellAssays #ColorimetricDetection #MicroplateReader #LabSupplies #LifeScienceResearch #CellSamples #FroggaBio #OrganoidResearch #BiotechProducts #InnovativeResearch #ResearchKit #LabEquipment #CellBiology #ScientificInstruments #LifeSciences #LabKits #ResearchTools #BiomedicalResearch #LabTesting #CellProliferation