Promega Ireland’s Post

Assess cell proliferation in under two hours with our "Add-Mix-Measure" Lumit® hKi-67 Immunoassay. This simple, no-wash, plate-based solution tracks cell proliferation using only a luminescence reader More at: https://bit.ly/4173o2j #assays #immunoassay #CellProliferation

Want to find antigen-specific TCR/BCR clonotypes - specific, sensitive, all-in-one workflow

Optimizing Cell Density: A Key to Robust Cell-Based Assays Proliferation and Viability As you explore cell densities for assay development, tracking cell proliferation and viability yields valuable insights. Cell viability assessments utilizing indicators like ATP levels or dyes that distinguish between live and dead cells offer insights into density-related phenomena, such as sparse or overcrowded conditions that can prompt cellular demise. Among the frequently employed dyes are Calcein-AM, utilized for live cell detection, and propidium iodide, ethidium homodimer, or trypan blue, employed for identifying dead cells. Proliferation assays such as BrdU or EdU incorporation can provide quantitative measures of expected cell division activity. Additionally, cell-cycle markers of proliferating cells, such as Ki67, can also be used to identify proliferating vs. senescent cells. #CellBasedAssays #CellDensity #AssayOptimization #CellProliferation #CellViability #BrdU #EdU #ProliferationAssays #ViabilityIndicators #TrypanBlue #ATPLevels #ExperimentalDesign #DataAnalysis #BiomedicalResearch #LaboratoryTechniques #ResearchMethods #CellularHealth #OptimizationStrategies #BenchtopScience #ScientificMethods

This article shows that the G-Rex method reduces the residual TCR/CD3+ contaminations 45x more than their previous method while expanding the target CAR T cells, yielding in an ultrapure product of 99.99 % The new depletion method used focuses on coculturing target CAR T cells with an NK-92 cell line in G-Rex to purify their CAR T cells and promote CAR T cell expansion at the same time. Download the entire study here: https://lnkd.in/gqYiARvC #celltherapymanufacturing #CART #celltherapy #biotech

Seahorse Real-Time Cell Metabolic Analysis With over 20,000 genes, 200,000 proteins and thousands of pathways, you can't measure everything in a cell at once, but you can measure the energy that drives them. Agilent offers cell analysis technology solutions that detect discrete changes in cellular bioenergetics in real-time. Real-time cell measurements provide a clear window into the critical functions driving cell signaling, proliferation, activation, toxicity and biosynthesis. Move beyond analyzing what your cells are, and reveal a clearer measure of what they do.

Not sure where to begin with your single-cell multiomics experiments? Explore our detailed video guides and learn from our experts about important topics like antibody titration, cell hashing, and data analysis. https://bit.ly/4hDghHc

JAK inhibitors in GCA? We have a phase 3 trial in giant cell arteritis back on the opening day plenary stage. The SELECT-GCA study, looking at upadacitinib in GCA, was scheduled to anchor the #ACR24 opening plenary for a reason - it is highly notable and badly needed. https://buff.ly/3B1hNSU

Rethinking Cell Proliferation Assays Discover a better way to measure cell proliferation. Promega’s Lumit® hKi-67 Immunoassay offers accurate results by tracking the Ki-67 marker, regardless of metabolic state. It's fast, efficient, and no-wash – perfect for your research needs. More at: https://bit.ly/4btJz6Y #assays #immunoassay #CellProliferation

Please checkout how modulating plaque cell phenotypes could revolutionize therapeutic strategies for advanced atherosclerosis in our latest review! Dive into the cutting-edge insights now! https://lnkd.in/gHis8vm3

👨🔬 👩🔬 Cell proliferation / cytotoxicity / viability detection methods and tools for your cell research 👇 🔎 🔬 𝐓𝐡𝐞 𝐒𝐢𝐦𝐢𝐥𝐚𝐫𝐢𝐭𝐢𝐞𝐬 𝐚𝐧𝐝 𝟖 𝐃𝐢𝐟𝐟𝐞𝐫𝐞𝐧𝐜𝐞𝐬 𝐁𝐞𝐭𝐰𝐞𝐞𝐧 𝐌𝐓𝐓 𝐚𝐧𝐝 𝐂𝐂𝐊-𝟖 1️⃣ MTT Cell Proliferation and Cytotoxicity Assay Kit  🔗 https://lnkd.in/eYXmg4jr 2️⃣ Enhanced Cell Counting Kit 8 (WST-8/CCK8)   🔗 https://lnkd.in/gWeuawB8 #CellProliferation #CellCytotoxicity #CellDetection #MTT #CCK8 #CellCounting