Opatola James’ Post

Here is the final open access version of our newly accepted article. "In silico and in vitro analysis of dipeptidyl peptidase-IV and angiotensin-converting enzyme inhibitory peptides derived from milk lactoferrin" The study explores the inhibitory potential of bioactive peptides from lactoferrin (LF), a versatile milk glycoprotein derived from bovine and human milk, against angiotensin-converting enzyme (ACE) and dipeptidyl peptidase-IV (DPP-IV). Firstly, in silico digested LF sequences were screened for potential ACE and DPP-IV inhibitory activity using AHTpin and StackDPPIV, respectively. Molecular docking showed that bovine LF peptides, “EPYF” (−10.1 kcal mol−1) and “WQWR” (−9.3 kcal mol−1), had significantly lower binding energies with ACE and DPP-IV, respectively. In addition, in vitro analysis of bovine LF hydrolysates displayed an IC50 value of 0.48 ± 0.01 (ACE) and 0.93 ± 0.02 mg mL−1 (DPP-IV). Liquid chromatography-mass spectrometry (LC-MS/MS) analysis identified ACE and DPP-IV inhibitory peptides, “EPYF” and “WQWR”, which were present within the parent peptide sequences of the bovine LF hydrolysates. Considering the potential bioactivities of bovine LF peptides and their functional similarities with human LF, bovine LF could be exploited as a promising alternative to human LF. Durban University of Technology DUT_Biotechnology and Food Science . The National Research Foundation of South Africa (NRF) Cherise Elisha Prashant Bhagwat Santhosh Kumar Pillai #Research #BioactivePeptides #Nutraceuticals #FunctionalFoods #Biotechnology

Struggling with outdated endotoxin testing methods? Jordi Iglesias Cullell from Charles River Laboratories will showcase a new statistical data analysis and validation approach for a Recombinant Cascade Reagent at the USP Virtual Workshop on May 15. Learn how to and streamline your testing processes for better compliance and faster results. Register today! https://okt.to/hbzecx #usp #endotoxin #microbiology

Minimum inhibitory concentration; the lowest concentration (in μg/mL) of an antibiotic that inhibits the growth of a given strain of bacteria. Can you read it? What’s the MIC? #microbiology #mls #bacteriagrowthinhibition #antibiotics

An interesting read about the enhancement of Sialyation of Fusion glycoproteins in rCHO cells..!!

In situ chemoproteomic profiling reveals itaconate inhibits de novo purine biosynthesis in pathogens #CellReports #microbiology https://hubs.li/Q02S4mmd0

In clinical microbiology, the isolation and culture of bacteria are fundamental processes that significantly influence diagnostic outcomes. 🔗 https://buff.ly/3Piiyde To read the full article please follow the link here: #samplecollection #diagnostics #NHS #ClinicalMicrobiology #Microbiology #bacteria #incubation #anaerobes

TraDIS-Xpress, offered at QIB Extra, is a transposon-directed insertion-site sequencing service. It enables the identification of bacterial survival mechanisms in diverse environments, providing insights for drug discovery, strain optimisation and antimicrobial efficacy. Explore the science behind TraDIS-Xpress with Emma Holden and Muhammad Yasir in this informative clip. Connect with us to delve into the transformative capabilities of TraDIS-Xpress in microbiology. 🧫🔍 #TraDISXpress #Microbiology #ResearchScience #QIBExtra #QIB #transposon #sequencing

Every Monday morning I make myself a coffee to get things started. Afterwards, I search the internet for interesting news in CHO cell culture just to stay ahead. Here is what kept me reading today: Ever wondered what keeps therapeutic glycoproteins in the game longer in your body? It's all about the sialylation! That's right, the glyco-wizardry that makes your meds last longer is a hot topic in biotech, and here's the scoop from the latest research: They took a deep dive into the world of recombinant Chinese hamster ovary (rCHO) cells and explored how sialyllactose could amp up the sialylation of Fc-fusion glycoproteins. Why? To make your meds not just good but GREAT! Here's the breakdown: Two sialyllactoses, 3'-SL and 6'-SL: They added these magic sugars to rCHO cell lines (DUKX-B11 and DG44) producing Fc-fusion glycoproteins. Results: Boom! Increased sialylation across the board. More isoform distribution, more sialylated N-glycans, and higher sialic acid content. How?: More intracellular CMP-sialic acid (CMP-SA) – the MVP of sialylation. Comparison: Their sialyllactoses went head-to-head with N-acetylmannosamine (ManNAc), and the results? Pretty darn close! Versatility: Worked wonders in three different commercially available CHO cell culture media. "Easy" to try by yourself. 📊 The verdict? Enzymatically-synthesized sialyllactose is a rockstar candidate for boosting glycoprotein sialylation in rCHO cultures. This could be a game-changer for the biotech world, paving the way for more efficient and longer-lasting therapeutic proteins. Paper by Hoon-Min Lee and colleagues: https://lnkd.in/evzXDv-A #Biotech #Glycoproteins #Sialylation #rCHOCells #TherapeuticProteins #Innovation #ScienceMagic #Biopharma

Happy to share our recent book chapter in book entitled Microbial Products for Health and Nutrition https://lnkd.in/dFDRS3mC #Indrashiluniversity #IU #lifesciencesuniversity #GSBTM #Cadilapharmaceuticals #Departmentofbiosciences #SchoolofScience #Microbiology #Biotechnology

Recombinant AKT3 Protein