Octet®- Affinity, Binding Kinetics and Concentration’s Post

How can timing impact API characterization? Combining various instruments previously discussed in the Crystal Classroom series, Subject Matter Expert, Derik McCarthy, outlines important considerations for time dependence with: 🔹 XRPD 🔹 TGA 🔹 DSC Subscribe on YouTube to keep up with the series: https://lnkd.in/dNFW-DFA #CrystalPharmatech #CrystalClassroom #XRPD #Chemistry #PharmaceuticalScience #CMC #Biotech #DrugDevelopment #Research #Technology #API #CRO #CDMO

A compound's lipophilicity can provide an indication of how it may interact in the human body. Traditionally, lipophilicity was measured by the time-consuming “shake flask method” however today, chromatographic and miniaturised shake flask methodologies offer a quicker alternative. Reversed phase high performance liquid chromatography (RP-HPLC) enables rapid, scalable lipophilicity screening by comparing a compound's retention time against standards with known hydrophobicity. Quickly determining key indicators of interactions through HPLC lipophilicity can help compounds progress through the drug discovery pathway. Find out more about our chromatographic LogD assay here: https://lnkd.in/e-xQYXWp #adme #dmpk #invitro #assays

Chemistry plays a major role in developing a manufacturable Antibody-drug conjugate (ADC). From the design and optimization of synthetic routes to developing novel linker-payloads, these challenging steps require extensive expertise in order to deliver a scalable and high-quality ADC.   Our latest info sheet, "Complex Chemistry for Antibody-Drug Conjugates and Beyond," explores how Abzena’s advanced chemistry solutions drive the rapid development of highly potent compounds and bioconjugates. Our expertise across small molecules, peptides, and oligos coupled with our integrated approach, enables us to provide innovative strategies that confidently advance ADC programs from discovery through clinical and commercial stages.   Read the full info sheet to discover how our specialized chemistry capabilities can streamline your ADC's development journey: https://lnkd.in/eNR9hn72   #ADCDevelopment #ComplexChemistry #DrugDevelopment #Bioconjugation #CDMO #CRO

-miRNA(microRNA) : 21-25nt, single-stranded RNA molecule that acts on the 3'UTR of mRNAs -siRNA(Small interfering RNA): 21-23nt, double-stranded RNA molecule, acts on mRNAs Modification of siRNA strand: 2‘-OMe -Improved siRNA resistance to nuclease and increased stability  2‘Fu，FC -Improved siRNA resistance to nuclease and increased stability  PEG -Enhanced cellular uptake of siRNA and delayed half-life of siRNA in serum  Peptide -Enhanced cellular guidance and uptake of siRNA, etc.  #siRNA #RNAresearch #RNA #sgRNA #miRNA #RNAsynthesis

The structure and spectroscopic properties of 5 methyl 2 8 quinolinyl benzoxazole and its complexes with Zn(II ion were studied using a DFT and TD DFT methods with def2 TZVP basis set. It was shown that the type of functional used B3-LYP or pbe0 implemented in TURBOMOLE package does not have essential influence on the geometry small differences in bond length valence and dihedral angles of studied compounds in both ground and excited states Link :- https://lnkd.in/eGKdSRqm #CADD #MedChem #CompChem #DrugDiscovery #Pharma #Biotech #CRO #innovation #digitalmarketing #technology #education BIOVIA Zastra Innovations

Ion-exchange centrifugal partition chromatography (CPC) offers industrial-scale production of oligonucleotides with high purity and yield in a single purification step in the pharmaceutical and biotechnology industries. As a liquid-liquid chromatographic technique, CPC uses a two-phase solvent system, which means it uses no solid stationary phase, such as silica gel. Paired with its solvent recycling capabilities, these traits make CPC exceptionally cost-efficient. Watch the video below for more information, and click the link to download the free application note. https://lnkd.in/dQyivDRv #chromatography #nucleotide #oligonucleotide #laboratory #pharma #downstreamprocessing #purification #continuousmanufacturing #liquidchromatography #continuousbioprocessing #bioprocessing #pharmaceutical #pharmaceuticalindustry #lipidresearch #drugdelivery #drugdevelopment #manufacturing #downstreamprocessing #GMP #biopharma #biotech #biotechnology #MethodDevelopment #LabInnovation

VCD can be applied to structural problems involving amino acids, peptides, polypeptides, proteins, carbohydrates, molecules of pharmaceutical interest, natural products, nucleic acids, as well as chiral molecules of interest to organic or inorganic chemists. It can also be used for finding structure elucidation and the determination of the stereo-conformational features of chiral molecules. Since the CD signals in the infrared region are one or more orders of magnitude lower than ECD signals in the UV-Vis region, high sensitivity, and stability are required for a VCD spectrometer. The FVS-6000 & VFT 4000 are the VCD spectrometers of choice for highly sensitive VCD measurements. JASCO Corporation is a pioneer in circular dichroism spectrometry and Jasco FVS-6000 is an essential tool for all absolute configurations of molecules. For more details visit https://lnkd.in/g6z3gg-b... For any information and inquiries mail us at [email protected] #vcd #stereochemistry #vibrationalspectroscopy #spectroscopy #circulardichroism 

Check out our latest article in Drug Discovery Today, titled "Exploring the Chemical Space of Orally Bioavailable PROTACs" together with Giulia Apprato, Diego García Jiménez, Yoseph Atilaw, Mate Erdelyi, Giuseppe Ermondi, Giulia Caron and Jan Kihlberg Take home: • Most of the reported orally bioavailable proteolysis targeting chimeras (PROTACs) are based on #cereblon. • The oral druggable space of PROTACs is identified better by 3D descriptors than by 2D descriptors. • #PROTAC lipophilicity/polarity and #chameleonicity can be optimized by chromatography. • In silico methods show promise for the prediction of 3D descriptors of PROTACs. 📰 Read the full article here: https://lnkd.in/dCwKKKkW 💻🔬 #DrugDiscovery #PROTACs #chemicalspace #cellpermeability #NMR #chromatography

Two days ago, on the 4th of April, we celebrated National Vitamin C Day 🍋 🍊 🥝. Aside from those sparkly crystals set in jewellery pieces, what about pharmaceutical crystals? How does a Vitamin C crystal form? What about its nucleation? New application note released by Technobis Crystallization Systems "Automating Nucleation Kinetics Analysis With Crystal16: Effect of Solvent on Nucleation'' observes the nucleation kinetics of ascorbic acid in water and ethanol-water binary solvent systems by the isothermal method. Correct, with today's state-of-the-art technologies, the moment when you can grow a perfect crystal in the lab using volume of 1mL is here. Also today, on 6th of April and 223 years later, we remember the birthday of the founder of modern crystallography, prof. William Hallowes Miller. Miller indices were written so long ago, and become even more important today. Herbal-ID Technobis Crystallization Systems #crystallization #analysis #nucleation #crystals #pharmaceuticals #api #pharma #kinetics #polymorphism #medicines #API #analytical #analyticalmethod https://lnkd.in/gaqgaaD5

A fantastic contribution to the synthesis of fully substituted hindered primary amines by #DanLehnherr's team at #Merck and #TomislavRovis's team at #ColumbiaUniversity! #Amines are crucial structural motifs in active pharmaceutical ingredients (#APIs) and their intermediates, as well as key components in ligand libraries for both biological and catalytic applications. You can explore the full publication here: https://lnkd.in/g7_4yySd Key Highlights: 1. Electrochemical Method: A novel electrochemical approach is described to access hindered primary and secondary amines, utilizing benchtop-stable iminium salts and a cyanoarene as starting materials. 2. Mechanistic Insight: This process involves proton-coupled electron transfer (PCET) followed by heteroradical−radical coupling, with subsequent re-aromatization leading to the final products. 3. Substrate Scope: At least one aryl group is required to stabilize the α-amino radicals in the imine; alkyl iminium salts are incompatible due to lack of radical stabilization. 4. Critical Parameters: The choice of cathode material and the supporting electrolyte are crucial for the success of this reaction. Key Merits: 1. A versatile and practical approach: This method accommodates a broad range of substituted heterocycles—including pyridine, pyrimidine, pyrazine, purine, and azaindole—bearing functional groups such as halides, trifluoromethyl, esters, amides, ethers, heterocycles, as well as unprotected alcohols and alkynes. #ProcessDevelopment #DrugDevelopment #DrugSubstances #CDMO #CRO #networking #Pharma #Biotech #CMC #SmallMolecules #Intermediates #API #manufacturing