Madhav Naik’s Post

Assessment of Membrane-Based Downstream Purification Processes as a Replacement to Traditional Resin Bead for Monoclonal Antibody Purification In this study, ion exchange and protein A membrane chromatography performances were assessed and compared with their resin counterparts. Protein A dynamic binding capacities were higher than 50 g/L for two of the tested membranes and with a residence time of 0.2 min. For polishing, it was observed that aggregate clearance was generally less performant with membrane separation when compared to resins with similar ligands. However, the comparable yield and increased productivity of membranes could be enough to consider their implementation. In addition, lifetime studies demonstrated that the performance of membranes remained robust over cycles. One hundred cycles were reached for most of the tested membranes with no impact on the process performance nor product quality. https://lnkd.in/gyjmMNVd #aspenalert #biotech #bioprocess

Membrane #chromatography. Have you heard of it? 📰 Faster. Cheaper. Better. 🙌 Oh 💡 You also won't need crazy packing procedures / equipment / qualified packing operators and 100's upon hundred's of liters of buffer. Do you want to hear more 🤔 #together👫 we can..... 🟡 Simplify Progress 🟡

A Detailed Assessment of Membrane-Based Downstream Purification Processes. Clear advantages compared to traditional resin-based processes. And there is more to it: Innovation in the field of membrane-based chromatography won't stop here.

Peptide purification #biotage Biotage

🚀 #TIDESUS is approaching fast! 📆 May 14th – 17th. 🔷 Hynes Convention Center, Boston As global leaders in the production of solid phase supports for #peptide and #oligo synthesis, and IEX resins for purification, Sunresin colleagues Yaping Hou, Alessandra Basso, Silvia Baldina, Simona Serban and Patrick Wang are excited to discuss your process needs at booth 920. Be sure to also check out posters 118 and 119, presenting data for our proprietary manufacturing process for mono-sized beads, and innovative new resins for improved #synthesis of large molecular weight peptides. See you there! 🤝 #SPPS #SPOS #IonExchange #Chromatography 

Its important to have a stable kit that can measure intra-cellular ATP consistently. Do check our #cell #viability #assay kit below

Hello, glycosidically oriented scientists. Progress of your projects with glycosides can be accelerated by reading a post from scimers.com referreing a recent research article on N-glycosides.

The field of #oligonucleotide therapeutics has seen significant advancements, resulting in a growing demand for purified oligonucleotides. 📈   One established method for oligonucleotide production involves chemical synthesis followed by chromatographic #purification. For efficient upscaling, ion exchange #chromatography is preferred due to its high loading capacity, resolution, and yield. 💡 BianoGMP GmbH specializes in producing high-purity oligonucleotides in substantial quantities, catering to research and development as well as clinical programs. In our application note, we demonstrate a successful large-scale ion exchange chromatography purification of an oligo dT with 95% purity and 90% yield. 🔬   Find out more about large scale purification of oligonucleotides with ion exchange chromatography in our application note. 👇   #thinklc #thinkknauer #application #practice Ulrike Krop Tobias Pöhlmann

High-Density Perfusion Cultures of the Marine Bacterium Rhodovulum Sulfidophilum for the Biomanufacturing of Oligonucleotides In this work, perfusion cell cultures were established for the intensification of ON biomanufacturing. First, the perfusion conditions were simulated in 50 mL spin tubes, selected as a scale-down model of the process, with the aim of optimizing the medium composition and process parameters. This optimization stage led to an increase in the cell density by 44 % compared to the reference medium formulation. In addition, tests at increasing perfusion rates were conducted until achieving the maximum viable cell density (VCDmax), allowing the determination of the minimum cell-specific perfusion rate (CSPRmin) required to sustain the cell culture. Intriguingly, we discovered in this system also a maximum CSPR, above which growth inhibition starts. https://lnkd.in/gyZ_2wEx #aspenalert #biotech #bioprocess

Discover the ion exchange #chromatography for large scale #purification of #oligonucleotides by KNAUER. Benefit from high loading capacity, resolution, and yield. 💡 In the application note with BianoGMP GmbH, KNAUER demonstrates a successful large-scale ion exchange chromatography purification of an oligo dT with 95% purity and 90% yield. 👇 #thinklc #thinkknauer Ulrike Krop Tobias Pöhlmann