Logos Biosystems’ Post

Structured RNAs have complex electrostatic topologies that influence how small molecule drugs find their targets. Small molecules conduct a low-pass flyover of neutral and negatively charged ridges and valleys en route to their target. Once they arrive, (they may have to displace a water or two!), complementary hydrogen bonding and the hydrophobic effect anchor them in place. As the community strives to develop new RNA targeted molecules I think it is important to remember that RNA folding is driven by enthalpic contributions, the sum of many non-covalent interactions, not the hydrophobic effect as protein folding is. The rules of the game may be, and likely are, different.

This article shows that the G-Rex method reduces the residual TCR/CD3+ contaminations 45x more than their previous method while expanding the target CAR T cells, yielding in an ultrapure product of 99.99 % The new depletion method used focuses on coculturing target CAR T cells with an NK-92 cell line in G-Rex to purify their CAR T cells and promote CAR T cell expansion at the same time. Download the entire study here: https://lnkd.in/gqYiARvC #celltherapymanufacturing #CART #celltherapy #biotech

Our cell signaling assays use the power and convenience of bioluminescence to measure the activity of various enzymes and proteins involved in signal transduction cascades. Browse the full range for detection of ADP, cAMP, GPCR GTPase and other enzyme activities: https://bit.ly/3Ti9JlP #lifescience #biotech #bioluminescence

Join us as we provide guidance and detailed recommendations for optimizing image-based cell migration assays within microplate formats. We’ll consider several factors, such as biological context/sample type, throughput, cost, and desired readout in our discussion. https://lnkd.in/dv5cmrDF #Agilent #Cell #Cellmigration

New to 3D Cell Culture? Learn about 3D cell cultures, why and how they are used, and how to choose assays for 3D models on our website, which includes a free 3D Culture Guide at: https://bit.ly/3SQZ1DE #3DCellCulture #CellCulture #spheroids

New to 3D Cell Culture? Learn about 3D cell cultures, why and how they are used, and how to choose assays for 3D models on our website, which includes a free 3D Culture Guide at: https://bit.ly/46ivAjj #3DCellCulture #CellCulture #spheroids

Achieving accurate quantitation requires robust bioanalytical methods. Harness the power of mass spectrometry for unparalleled sensitivity, specificity and speed. Explore our instruments: -Sciex API 6500l with Selexion Ion Mobility: Exceptional sensitivity and selectivity. Ion mobility for advanced separation, enhancing throughput. -Thermo Orbitrap Exploris 240: High-res mass spectrometry with precision. Versatile for diverse applications, especially with large molecules -Agilent 7900 ICP/MS: Precision ideal for quantitative analysis and nanoparticles. Well-suited for targeted screening scenarios. Improve the accuracy of your nucleotide screening with Sannova #BioanalyticalCRO #ScreeningNucleotidePlates #DrugDevelopment #HighThroughputAssays

ICYMI: Single cell sequencing (#scRNAseq) has allowed us to capture cellular diversity in complex biological systems, both in vivo and in vitro. Read our blog that compares top platforms to power your next big discovery: https://lnkd.in/ekvx9NUg #singlecell #bioinformatics #bigdata

New to 3D Cell Culture? Learn about 3D cell cultures, why and how they are used, and how to choose assays for 3D models on our website, which includes a free 3D Culture Guide at: https://bit.ly/3Il5BfN #3DCellCulture #CellCulture #spheroids

Happy #fluoresence #friday! The calcein AM viability assay is a simple sensitive assay for measuring cell viability, cell proliferation , or to assess cytotoxicity in adherent cells or those in suspension. Calcein AM interacts with intracellular esterases to produce a hydrophilic, strongly fluorescent compound that is retained in the cytoplasm. Analysis can be performed at an Ex/Em = 490/525 nm in a fluorescent microplate reader or under an appropriate channel in a flow cytometer. Other calceins may require other spectral settings outside of the typical green wavelengths. The measured fluorescence intensity is proportional to the number of viable cells within a sample. Check out our experimental protocol for calcein AM assay https://lnkd.in/gHi27Rsw #fluorescence #protocol #biotechnology