'With its intuitive touchscreen interface and rapid processing, it delivers highly accurate cell counts in seconds,' remarked this researcher from Università degli Studi di Milano-Bicocca (Milan, Italy) of their LUNA-II automated cell counter. Talk about ❤️ LUNA love ❤️ #cellcounter #cellbiology #researcher #laboratory
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The Cyto-ID® assay described in this protocol is based on using a specific dye that selectively stains autophagic compartments, combined with an additional Hoechst 33342 dye for nuclear staining. The Cytation Imager then enables the software to determine the number of autophagosomes per nucleus in living cells. To learn more, view https://lnkd.in/gxMeAcvA Barbara Hochecker | Dr. Katja Matt | Alica Meßmer | Melanie Scherer | Jörg Bergemann | Albstadt-Sigmaringen University Follow Bio-protocol for more life-science research updates. #openaccess #reproducibility #lifesciences #research #cellbiology
Quantification of Autophagosomes in Human Fibroblasts Using Cyto-ID® Staining and Cytation Imaging: https://lnkd.in/gxMeAcvA Work by Barbara Hochecker, Dr. Katja Matt, Alica Meßmer, Melanie Scherer, and Jörg Bergemann at Albstadt-Sigmaringen University. Reviewed by Olga Kopach and Vishal Nehru. Follow Bio-protocol and visit https://lnkd.in/gR3QGYz6 to explore publishing with us. #openaccess #reproducibility #lifesciences #research #cellbiology
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#ARCSinternational #ScientificConferenceOrganization #ScientificEventOrganization excited to announce the our upcoming Conference. Join us for a series of groundbreaking events that will bring together global experts, researchers, and innovators in diverse fields,#EnvironmentalChange #ClimateAction #BreastCancerResearch #CellBiology #MolecularBiology #ARCSConferences2025 #GlobalSummit #ScientificInnovation #ResearchCommunity #ScientificConferences #ResearchConferences #VirtualConferences #InternationalConferences #2025
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Watch this quick video on how to use the LUNA Reusable Slide! A cost-effective and eco-friendly option compatible with the entire LUNA family of automated cell counters. Offering the flexibility of disposable slides and a reusable slide, you never have to worry about cross-contamination. It's the best of both worlds. #researcher #labinnovation #lifescience #scientist #researchanddevelopment #cellbiology #molecularbiology
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I'm excited to share our recent publication titled "Endosomal actin branching, fission, and receptor recycling require FCHSD2 recruitment by MICAL-L1," where I had the privilege of contributing as the second author alongside an amazing team of researchers. Our study highlights the critical role of FCHSD2 in coordinating with MICAL-L1 to promote actin branching and fission, key steps in receptor recycling. #Research #CellBiology #Science #Publication #ActinDynamics #Endocytosis #MolecularBiology
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Most research focuses mainly on #what_is_happening, but few studies address a combination of #what_is_happening and #how_it_is_happening. Regarding this, I was glad to attend a talk by #Dr_Saikat_Chowdhury, Senior Scientist at #CSIR_CCMB, on structural insights into the activation of the ARP2/3 complex in actin polymerization. It was an honor to discuss my work with him. The Arp2/3 complex (Actin-Related Protein 2/3) is a key protein that enhances actin polymerization through linear/branched structures, which play a crucial role in responding to intracellular signaling systems such as cell division, transcription, cell differentiation, proliferation, muscle contraction, invasiveness, and metastasis. During the talk, he presented cryoEM images and videos demonstrating how ARP2/3 contributes to actin polymerization, which provided us with a clear molecular-level understanding. I would also like to thank our #School_of_BioSciences_Technology (SBST), #VIT_Vellore for organizing such an excellent and informative talk on this hot topic by bringing in Dr. Saikat Chowdhury, sir. Thank you, Professor, for your insights on CryoEM, which have boosted me to work on CryoEM. #CryoEM #StructuralBiology #ActinPolymerization #Arp2_3Complex #CellBiology #ResearchTalks #MolecularBiology #SBST #VIT #CSIR #ScientificResearch #AcademicGrowth.
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✨Here are some examples of new snapshots of mitochondria captured under microscopes, revealing the fascinating complexity of the regulation of the mitochondrial function in cardiac cells. 🧬 Pictures by Manon Restituito 📸 #mitochondrial #bioscience #CRO #cell #science
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How Do Mitochondria Produce Energy? Mitochondria produce energy through a process called oxidative phosphorylation. This process takes place in the inner mitochondrial membrane and involves the electron transport chain and ATP synthase. High-energy electrons are transferred through a series of protein complexes, ultimately creating a proton gradient that drives the synthesis of ATP from ADP and inorganic phosphate. Explore more insights on the Cell Biology. Visit our blog for more interesting updates: https://lnkd.in/gwF94uD7 #mitochondria #mitochondrialdisease #mitochondrialhealth #cellularenergy #mitochondrialfunction #powerhouseofthecell #cellbiology #EnergyMetabolism #cellularrespiration #biologicalenergy #metabolicprocesses #mitochondrialhealth #mitochondrialresearch
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Studying the roles of extracellular vesicles in embryo-maternal communication: in this mini-literature review, Alireza Fazeli & Kasun godakumara at Estonian University of Life Sciences highlighted the emerging role of EV-mediated intercellular signaling as a novel aspect of embryo-maternal dialogue, underscoring the significance of EVs in securely transporting labile molecules, particularly microRNA, which is believed to be a critical component of this communication system https://lnkd.in/gQwAXeUf #extracellularvesicles #exosomes #miRNA #uptake #embryo #Vesiculab
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Amazing what Microscopy combined with imaging and 3D graphics can do for visualizing our cellular machinery. #microscopy #mitochondria #proteomics #imaging #graphics
🔋 Energy Production & the Mitochondria 🦠 Mitochondria produce energy ⚡ through oxidative phosphorylation, involving five membrane-bound complexes known as the respiratory chain. The arrangement of these complexes within the mitochondria has been debated, particularly their assembly into supercomplexes, or "respirasomes." Using in situ cryo-electron tomography 🧊🔭, researchers visualized the native structures of these complexes inside Chlamydomonas reinhardtii cells. The study revealed that ATP synthases and respiratory complexes are organized into separate membrane domains, with ATP synthases in curved regions and respiratory complexes I, III, and IV assembling into a respirasome in flat regions. Key Points 🔽 Energy production ⚙️: Oxidative phosphorylation relies on five membrane-bound complexes. Respirasomes 🔗: Complexes I, III, and IV form supercomplexes in flat crista membranes. Imaging 🧪: In situ cryo-electron tomography provided 5 Å resolution of the respirasome, showing electron carrier cytochrome c binding. Implication 🔍: A detailed structural model reveals how mitochondrial complexes interact to drive ATP production. Credit to Florent Waltz and their team for the amazing video and great research! #CellBiology #Biology #Science #Mitochondria
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The hallmark of the microBrain™ platform is the spontaneous and coordinated network activity exhibited by the cortical organoids. Using a calcium reporter, we can capture these coordinated network bursts in real-time. Calculating the fluorescence over time provides a visual waveform of the functional activity. Changes in the number, size, or shape of these waveforms indicate functional neural modulation or viability impacts. #microscopymonday #neuroimaging #microBrain #AxoSim #3Dorganoids #corticalspheroids #organoids #centralnervoussystem #CNS #peripheralnervoussystem #PNS #Biotech #drugdevelopment
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