Labena Slovenia’s Post

🌟New preprint: Pith cell responses to low red: far-red light in dicot stems. doi: https://lnkd.in/ez7dgPvU ------------------------------------------------------------------------------ 🍅This study investigated how tomato internodes adapt to low R:FR light conditions, focusing on cellular anatomy and gene expression changes.  By profiling the transcriptome in both whole internodes and hand-dissected pith tissue from the central cylinder, we identified transcription factors (TFs) that were significantly upregulated in response to low R:FR. We then characterized FR-responses in eight dicotyledonous species and constructed report lines in tomatoes. We discovered central cylinder gene expression patterns in SAS that are distinct from those of the entire internode, suggesting that some responses are unique and likely specific to vascular cell types such as pith.  ------------------------------------------------------------------------------ Special thanks to talented student Lotte van der Krabben and Technician who helps me all the time Yorrit van de Kaa.🌟 #PlantScience #PhDResearch #Transcriptomics #LightSignaling

Science Q: What is long-read genomic sequencing, and how does it improve on previous sequencing technologies? Long-read genomic sequencing is form of next-generation sequencing that works by sequencing lengths of DNA or RNA in long, unbroken strands. This is an improvement over older technologies that had to chop up genetic material into shorter fragments in order to sequence them. This innovative technology presents researchers with numerous benefits. It enhances the detection of isoforms and gene fusions, streamlines the resolution of long and repetitive genomic regions, and uncovers complex structural details that may be harder to detect in shorter fragments. We are proud to now be offering long-read sequencing at Wistar. Dive deeper into our Genomics Shared Resource here: https://lnkd.in/eHqQYnVB

3 Ways Molecular Biology is Transforming the World 🔬 CRISPR Gene Editing CRISPR isn't a kind of salad but a transformative gene-editing tool. It allows scientists to change DNA sequences with unprecedented precision. This could mean curing genetic diseases or helping crops thrive. It also raises ethical concerns. 🧬 Personalized Medicine Previously, drugs were prescribed based on a one-size-fits-all approach. But with personalization, we can now tailor treatment to patients' genetics. This means better response rates, less wasted medicine, and targeted treatment. 🦠 Fighting Disease Our understanding of diseases like cancer has skyrocketed in the last few years. True, we haven't found a cure but we're longer guessing how to treat it. With new tools like gene sequencing, we're able to develop more effective treatments for a variety of diseases. It's an exciting time to be alive, and even more exciting to see what future advancements molecular biology has in store for us. #molecularbiology #personalisedmedicine #crispr #advancementofscience

📢 Don’t Miss Out on This FREE Webinar: Intelligent Design of Promoters! 🧬 Unlock new possibilities in synthetic biology with expert insights from Dr. Alexander Kel, CEO and CSO of geneXplain GmbH. This completely #FREEwebinar will provide a deep dive into the critical role of artificial promoters in advancing gene therapy, metabolic engineering, and synthetic biological circuits. 💡 Key topics covered: 🔑 What are promoters and enhancers? 🔑 Why do we need artificial ones? 🔑 Applications in gene therapy, metabolic engineering, and synthetic biology. 🔑 Promoter Architecture: Core promoters, regulatory elements, transcription factor binding sites, and more. 🔑 Top Databases and Tools: #JASPAR #GTRD #TRANSFAC and #GenomeEnhancer 🔑 #AI and #MachineLearning: Predicting promoter activity while balancing strength and specificity. This free opportunity is essential for anyone looking to stay at the forefront of synthetic biology and bioinformatics. Don’t miss out! 🗓️ Date: [8th october 2024] ⏰ Time: [6PM CEST] 🎟️ Register now for FREE: [https://lnkd.in/dbbrvUDD] #SyntheticBiology #FreeWebinar #GeneTherapy #Bioinformatics #MachineLearning #AI #PromoterDesign #geneXplain #TRANSFAC #GenomeEnhancer #JASPAR #GTRD #Webinar #JASPAR #GTRD #Transcriptomics #Genomics #TFBS #TranscriptionFactors #SyntheticBiology #ClinicalResearch #Biotechnology #ArtificialPromoters #PromoterDesign #PrecisionMedicine

Embark on a revolutionary journey in molecular biology with Protoscript II Reverse Transcriptase, your key to unlocking unparalleled precision and efficiency in cDNA synthesis. Whether you are delving into gene expression analysis, RNA sequencing, or viral research, Protoscript II is your trusted companion, elevating your experiments to new heights. 𝐊𝐞𝐲 𝐅𝐞𝐚𝐭𝐮𝐫𝐞𝐬: 𝐄𝐧𝐡𝐚𝐧𝐜𝐞𝐝 𝐒𝐞𝐧𝐬𝐢𝐭𝐢𝐯𝐢𝐭𝐲: Protoscript II boasts heightened sensitivity, ensuring the detection of low-abundance transcripts with unparalleled accuracy.. 𝐕𝐞𝐫𝐬𝐚𝐭𝐢𝐥𝐢𝐭𝐲 𝐔𝐧𝐥𝐞𝐚𝐬𝐡𝐞𝐝: This reverse transcriptase accommodates a wide range of RNA inputs, enabling seamless integration into diverse experimental setups.  𝐄𝐱𝐜𝐞𝐩𝐭𝐢𝐨𝐧𝐚𝐥 𝐘𝐢𝐞𝐥𝐝 𝐚𝐧𝐝 𝐅𝐢𝐝𝐞𝐥𝐢𝐭𝐲: Experience robust cDNA synthesis with maximum yield and fidelity. 𝐄𝐟𝐟𝐢𝐜𝐢𝐞𝐧𝐭 𝐓𝐞𝐦𝐩𝐞𝐫𝐚𝐭𝐮𝐫𝐞 𝐓𝐨𝐥𝐞𝐫𝐚𝐧𝐜𝐞: Navigate through a spectrum of experimental conditions with ease. Protoscript II's temperature tolerance ensures optimal performance across a broad range, providing flexibility in experimental design. . To know more write to us at inquiry@zellebiotech.com . #zellebiotechnology #neb #nebindia #ecoli #research #science #nextgenerationsequencing #dnasequencing #rnaseq #reagents #highfidelity #epigenetics #phage #researchers #polymerase #rna #pcrtesting #pcr

A significant breakthrough in genomics has been achieved with the discovery of a previously hidden code within DNA, termed 'spatial grammar.' Researchers from Washington State University and the University of California, San Diego, have unveiled this complex layer of gene regulation, challenging existing paradigms about how genes are controlled. Published in Nature, this research reveals that gene activity is influenced not just by the presence of transcription factors but also by their spatial arrangement within the DNA sequence. Traditionally viewed as either activators or repressors, transcription factors have been found to exhibit more nuanced roles depending on their position relative to a gene's transcription start site. This positioning, or 'ambience,' dictates whether these factors enhance or inhibit gene expression. This discovery has profound implications for our understanding of genetic variations and their roles in diseases. It could reshape how scientists study gene expression and develop therapeutic strategies, offering new avenues for personalized medicine and genetic research. As we integrate this new 'spatial grammar' into our genomic studies, the potential to uncover more about gene regulation and its impact on health is immense. This advancement marks a pivotal moment in genetics and molecular biology. #Science #Research#Technology

Stepping up the spatial biology game with Visium HD from 10x Genomics! 🌟 Peek into the microscopic world like never before. Ready for a deep dive into gene expression mapping? Let's explore what's next together. #SpatialBiology #10xGenomics #VisiumHD #ScienceCool #biopharma #biopharmabytes #transcriptomics

🔬Introducing #BacterialMERFISH, a revolutionary method for multiplexed RNA imaging in bacterial cells from Vizgen co-founder Jeffrey Moffitt's lab at Boston Children's Hospital & Harvard Medical School. 🌟 Highlights of Bacterial-MERFISH: 🔧 Bacteria-optimized #ExpansionMicroscopy toolbox: Tunable expansion of individual bacteria up to 1000-fold in volume. 📈 High-throughput, spatially resolved profiling of thousands of transcripts within individual bacteria. 🔍 Accurate, high detection efficiency and low false positives, capable of imaging 100k+ cells. 💡 Key Findings: 1️⃣ In E. coli grown on glucose and xylose, a richer single-cell response was observed during the transition phase, revealing coordinated transcriptional bursts and exploration of various sugars. 2️⃣ Detailed organization of the E. coli transcriptome was mapped, uncovering extensive sub-cellular RNA localization patterns. 3️⃣ B. theta in the mouse colon adapts its gene expression to different niches, upregulating genes for harvesting mucus polysaccharides near the host mucus layer. This technique opens new possibilities for studying bacterial single-cell heterogeneity in complex, spatially structured environments. Whether it's specialization in biofilms, antibiotic resistance, or commensal communication, bacterial-MERFISH will be a powerful tool for diverse research questions. 📖 Dive into the details of this groundbreaking study! Read the full article on bioRxiv: https://hubs.ly/Q02K2xSj0 #BacterialResearch #SpatialTranscriptomics #ExpansionMicroscopy #Microbiology #Vizgen

Breakthrough in human artificial chromosomes 🔬🧬 Researchers from the University of Pennsylvania have developed a new method for creating human artificial chromosomes. This could revolutionize genetic therapies for cancer and also has potential applications in agriculture, to develop pest-resistant crops. 𝗪𝗵𝘆 𝘀𝗵𝗼𝘂𝗹𝗱 𝘆𝗼𝘂 𝗸𝗻𝗼𝘄❓ 1. This approach produces human artificial chromosomes as safer, more efficient platforms for therapeutic gene expression, contrasting current virus-based methods. 2. This advanced gene therapy opens the door to new treatments. 3. It could lead to better-engineered cell therapies for diseases like cancer. 𝗛𝗼𝘄 𝗱𝗼𝗲𝘀 𝗶𝘁 𝘄𝗼𝗿𝗸⚙️ - Researchers have been able to use single, long constructs of designer DNA. - This solves issues where chromosomes were joining together in unpredictable long series and rearrangements. - They were also able to produce chromosomes that could reproduce themselves during cell division. Investment theme: Genetic Therapies - Source: Penn Medecine News / Sci Tech Daily #investing #genetics #health Thematic #investment #Litrendingtopics PS: Did you know that "chromosome" comes from the Greek words for color (chroma) and body (soma) because these cell structures are strongly stained by colorful dyes used in research? (source: National Humane Genome Research Institute)

❓Have you ever used single-cell sequencing? 💡It's all about diving deep into transcriptomic profiles of individual cells using state-of-the-art sequencing technology. This gives us a clearer picture of how each cell works in its own environment! 🎯In IRA3P we optimized and implemented sc-RNA-seq to study the expression profiles of individual cells from human blood cells and tissues using 10X Genomics Chromium platform. scRNA-seq provides: ✅ Precision by allowing to analyze individual cells, revealing unique gene expression profiles ✅ High resolution by detecting subtle differences between cells that traditional bulk sequencing might not capture ✅ Insightful by providing a detailed understanding of cellular diversity with its microenvirnoment ✅ Diagnostic potential by offering insights into molecular basis and mechanism of the disease and potential therapeutic targets at a cellular level. 📰 Are you eager to know how we used this novel technology in our research? Check our high impact paper that utilized scRNA-seq: https://lnkd.in/g9Ue92tG https://lnkd.in/gqtZxhT5 https://lnkd.in/gxdQ8-X6 https://lnkd.in/g6NS-ZHc