Eric Lefkofsky’s Post

Asuragen was proud to share data at #ESMO from our latest innovation in #breastcancer research - a comprehensive methodology for targeted RT-qPCR monitoring of #ESR1 mutations in plasma. Our streamlined workflow utilizes both exosomal RNA and circulating cell-free DNA, and is capable of interrogating 11 of the most common and actionable mutations for consistent and specific results. The in development panel reliably and specifically identifies rare variants in both contrived and clinical liquid biopsy samples using an in-house method (ExoLution Plus) designed to simultaneously enrich exoRNA and cfDNA, included with the QuantideX qPCR ESR1 exoMutation Kit (RUO). See the results below! https://lnkd.in/ge8qF9Qi

🔎 Simultaneously detect all major types of genomic rearrangements that cause and characterize #cancer on a genome-wide scale. Cytogenetics tool, CytoTerra, comprehensively surveys the genomic landscape from a wide variety of biological samples. Here’s how… Enabled by Genomic Proximity Mapping™, CytoTerra provides a high-resolution view of translocations and inversions, as well as copy number variants (CNVs), using a single NGS-based assay and cloud-based analysis platform. Discover more in the link below 👇

Sarah-Jane Dawson’s team has developed MisMatchFinder, a tool to detect mutational signatures in circulating tumor DNA using low-coverage whole-genome sequencing. MisMatchFinder removes non-tumor-related noise, such as germline variants and sequencing errors, enabling mutational signatures detection even at ultra-low coverage (down to 0.5×) in cfDNA samples with low tumor purity. Key Points: - Tested on 375 plasma samples across nine cancer types, with high concordance to traditional high-coverage sequencing. - Identifies mutational signatures, including APOBEC activity (SBS2), UV damage (SBS7), and a challenging "flat" mutation profile like homologous recombination deficiency (SBS3). - Powered by Rust and Echtvar, MisMatchFinder is blazingly fast. Paper: https://lnkd.in/gshjEzvm MisMatchFinder repo: https://lnkd.in/gN4xk6WV Echtvar processes up to 1 million variants per second, with gnomAD data storage reduced to 1.2 bits per variant: https://lnkd.in/gCzWkANQ #cfDNA #ctDNA #LiquidBiopsy #MutationalSignatures

MisMatchFinder: a tool to detect mutational signatures in circulating tumor DNA using low-coverage whole-genome sequencing. #liquidbiopsy #lowcoveragewgs

Tune in to this featured webinar to discover a pan-cancer #NGS assay for comprehensive genomic profiling (CGP) of solid tumor samples. Drive lab productivity with walkaway automation on the #Agilent Magnis NGS Prep system. https://bit.ly/3zkOUQm

Come meet Jin Li at AACR to learn about how ICON plc can support your sequencing needs.

Exciting to see the NEAT Liquid Biopsy Kit used in this study to concentrate and extract ESR1 mutations in plasma samples. Interested in learning more about this solution? Let's connect at [email protected] #nanotrap #liquidbiopsy #cancerresearch

Comprehensive genomic profiling optimizes cancer testing by covering all clinically relevant genes, including biomarkers that can influence participation in clinical trials. Neo Comprehensive™ – Solid Tumor includes 517 genes by DNA for SNVs and InDels, 59 genes by DNA for CNVs, and 55 genes by RNA for known and novel fusions and splice variants: https://lnkd.in/gPM6f7kv #CGP #ClinicalTrials #SolidTumor https://lnkd.in/g-ccT8EN

✨New Application Note!✨ GT Molecular's RUO ESR1 Mutation Panel Kit for the Roche Digital LightCycler® PCR System can detect common ESR1 mutations in one reaction with ultra-high sensitivity and specificity. Contact us to learn more and get a copy of this application note covering use of this impressive kit on an impressive platform with real plasma sample detection and concentration protocol analysis.   🤹♂️Highly multiplexed assay measures 9 ESR1 mutations and a wild-type ESR1 target within a single well 🔎Patent-pending technology allows for highly specific detection and quantification in both plasma and tumor tissue samples 📄Kits include controls and step-by-step data analysis guidance 🧬Assay detects key ESR1 mutations in ctDNA even in very low concentration plasma samples 💪Robust concentration protocol outlined within this study Learn more or contact us here: https://lnkd.in/gdRQQ6m8   #LiquidBiopsy #ISLB, AACR Journals, Roche, Ceres Nanosciences, Inc. #GTMolecular #ESR1 #BreastCancer #CancerResearch #nanotrap #roche #dPCR #digitalPCR #PCR

The Agilent TapeStation systems are automated electrophoresis instruments that provide objective analysis of nucleic acid samples. The TapeStation system uses the Agilent Genomic DNA ScreenTape assay to assess the sample size and concentration of input genomic DNA (gDNA) and generates a DNA quality metric called the DNA Integrity Number (DIN). The DIN assigns a score from 1 to 10 to each sample analyzed, with 1 indicating low-quality samples, and 10 indicating samples of the highest quality. Using 4,000 samples and their sequenced data collected by the German Cancer Research Center (DKFZ) and their associated input gDNA DIN values as an example, this application note shows users how the DIN can be used to differentiate between samples of different quality. By defining the quality of each input gDNA sample, users can define a threshold to determine if the sample is fit for NGS or not. Read more: https://lnkd.in/ddN42imQ