Aspen Alert’s Post

Enhancing Biomanufacturing Efficiency: A Model-Based Plug-and-Play Hybrid Fed-Batch Process Using ATF Perfusion for High-Yield Drug Substance Production

Process Optimization and Economic Evaluation of Continuous Multi-Column Capture for Monoclonal Antibody – A Case Study Protein A affinity chromatography has been widely employed as the capture step for mAb purification and serves as a key driver of economic efficiency on downstream operations. To enhance the purification process and alleviate manufacturing constraints while reducing costs, continuous processing, such as multi-column capture (MCC), has gained attention. This study aimed to establish and optimize the protein A MCC process and to evaluate it with traditional single-column capture mode. Through a series of verification runs, it was determined that there was no significant difference in yield and quality attributes across different loops. Furthermore, annual cost of goods (COGs) was evaluated with the conventional single-column batch process. The results showed that MCC process reduced operation cost by 18% and almost doubled productivity in the specific scenario. https://lnkd.in/gbACvsCV #aspenalert #biotech #bioprocess

𝐓𝐑𝐈𝐍𝐈𝐓𝐘 𝐂𝐡𝐚𝐫𝐠𝐞 𝐏𝐥𝐮𝐬 𝐋𝐞𝐧𝐭𝐢𝐜𝐮𝐥𝐚𝐫 𝐅𝐢𝐥𝐭𝐞𝐫𝐬: 𝐀 𝐏𝐨𝐰𝐞𝐫𝐟𝐮𝐥 𝐒𝐨𝐥𝐮𝐭𝐢𝐨𝐧 𝐟𝐨𝐫 𝐁𝐢𝐨𝐩𝐡𝐚𝐫𝐦𝐚𝐜𝐞𝐮𝐭𝐢𝐜𝐚𝐥 𝐚𝐧𝐝 𝐁𝐢𝐨𝐭𝐞𝐜𝐡 𝐀𝐩𝐩𝐥𝐢𝐜𝐚𝐭𝐢𝐨𝐧𝐬 TRINITY Charge Plus Lenticular Filters are cutting-edge filtration solutions designed to meet the stringent requirements of the biopharmaceutical and biotech industries. These positively charged cellulose depth filters are formulated to deliver exceptional performance and reliability. 𝐊𝐞𝐲 𝐅𝐞𝐚𝐭𝐮𝐫𝐞𝐬 𝐚𝐧𝐝 𝐁𝐞𝐧𝐞𝐟𝐢𝐭𝐬: 𝐌𝐞𝐜𝐡𝐚𝐧𝐢𝐜𝐚𝐥 𝐚𝐧𝐝 𝐄𝐥𝐞𝐜𝐭𝐫𝐨-𝐊𝐢𝐧𝐞𝐭𝐢𝐜 𝐀𝐝𝐬𝐨𝐫𝐩𝐭𝐢𝐯𝐞 𝐂𝐚𝐩𝐭𝐮𝐫𝐞: Effectively removes particulate matter, microorganisms, colloids, and pyrogens from critical process streams. 𝐄𝐧𝐡𝐚𝐧𝐜𝐞𝐝 𝐅𝐢𝐥𝐭𝐫𝐚𝐭𝐢𝐨𝐧 𝐄𝐟𝐟𝐢𝐜𝐢𝐞𝐧𝐜𝐲: Superior performance and reduced pressure drop. High Flow Rates: Optimized for efficient processing. For Know About Carbon Lenticular Filter https://buff.ly/40xOHF2 For more information and to explore how TRINITY can support your filtration needs, please contact us at [email protected] #TRINITY #lenticularfilters #biopharma #biotech #filtration #pharmaceuticalgrade #qualitycontrol #processoptimization

𝐓𝐑𝐈𝐍𝐈𝐓𝐘 𝐂𝐡𝐚𝐫𝐠𝐞 𝐏𝐥𝐮𝐬 𝐋𝐞𝐧𝐭𝐢𝐜𝐮𝐥𝐚𝐫 𝐅𝐢𝐥𝐭𝐞𝐫𝐬: 𝐀 𝐏𝐨𝐰𝐞𝐫𝐟𝐮𝐥 𝐒𝐨𝐥𝐮𝐭𝐢𝐨𝐧 𝐟𝐨𝐫 𝐁𝐢𝐨𝐩𝐡𝐚𝐫𝐦𝐚𝐜𝐞𝐮𝐭𝐢𝐜𝐚𝐥 𝐚𝐧𝐝 𝐁𝐢𝐨𝐭𝐞𝐜𝐡 𝐀𝐩𝐩𝐥𝐢𝐜𝐚𝐭𝐢𝐨𝐧𝐬 TRINITY Charge Plus Lenticular Filters are cutting-edge filtration solutions designed to meet the stringent requirements of the biopharmaceutical and biotech industries. These positively charged cellulose depth filters are formulated to deliver exceptional performance and reliability. 𝐊𝐞𝐲 𝐅𝐞𝐚𝐭𝐮𝐫𝐞𝐬 𝐚𝐧𝐝 𝐁𝐞𝐧𝐞𝐟𝐢𝐭𝐬: 𝐌𝐞𝐜𝐡𝐚𝐧𝐢𝐜𝐚𝐥 𝐚𝐧𝐝 𝐄𝐥𝐞𝐜𝐭𝐫𝐨-𝐊𝐢𝐧𝐞𝐭𝐢𝐜 𝐀𝐝𝐬𝐨𝐫𝐩𝐭𝐢𝐯𝐞 𝐂𝐚𝐩𝐭𝐮𝐫𝐞: Effectively removes particulate matter, microorganisms, colloids, and pyrogens from critical process streams. 𝐄𝐧𝐡𝐚𝐧𝐜𝐞𝐝 𝐅𝐢𝐥𝐭𝐫𝐚𝐭𝐢𝐨𝐧 𝐄𝐟𝐟𝐢𝐜𝐢𝐞𝐧𝐜𝐲: Superior performance and reduced pressure drop. High Flow Rates: Optimized for efficient processing. For Know About Carbon Lenticular Filter https://buff.ly/40xOHF2 For more information and to explore how TRINITY can support your filtration needs, please contact us at [email protected] #TRINITY #lenticularfilters #biopharma #biotech #filtration #pharmaceuticalgrade #qualitycontrol #processoptimization

𝐓𝐑𝐈𝐍𝐈𝐓𝐘 𝐂𝐡𝐚𝐫𝐠𝐞 𝐏𝐥𝐮𝐬 𝐋𝐞𝐧𝐭𝐢𝐜𝐮𝐥𝐚𝐫 𝐅𝐢𝐥𝐭𝐞𝐫𝐬: 𝐀 𝐏𝐨𝐰𝐞𝐫𝐟𝐮𝐥 𝐒𝐨𝐥𝐮𝐭𝐢𝐨𝐧 𝐟𝐨𝐫 𝐁𝐢𝐨𝐩𝐡𝐚𝐫𝐦𝐚𝐜𝐞𝐮𝐭𝐢𝐜𝐚𝐥 𝐚𝐧𝐝 𝐁𝐢𝐨𝐭𝐞𝐜𝐡 𝐀𝐩𝐩𝐥𝐢𝐜𝐚𝐭𝐢𝐨𝐧𝐬 TRINITY Charge Plus Lenticular Filters are cutting-edge filtration solutions designed to meet the stringent requirements of the biopharmaceutical and biotech industries. These positively charged cellulose depth filters are formulated to deliver exceptional performance and reliability. 𝐊𝐞𝐲 𝐅𝐞𝐚𝐭𝐮𝐫𝐞𝐬 𝐚𝐧𝐝 𝐁𝐞𝐧𝐞𝐟𝐢𝐭𝐬: 𝐌𝐞𝐜𝐡𝐚𝐧𝐢𝐜𝐚𝐥 𝐚𝐧𝐝 𝐄𝐥𝐞𝐜𝐭𝐫𝐨-𝐊𝐢𝐧𝐞𝐭𝐢𝐜 𝐀𝐝𝐬𝐨𝐫𝐩𝐭𝐢𝐯𝐞 𝐂𝐚𝐩𝐭𝐮𝐫𝐞: Effectively removes particulate matter, microorganisms, colloids, and pyrogens from critical process streams. 𝐄𝐧𝐡𝐚𝐧𝐜𝐞𝐝 𝐅𝐢𝐥𝐭𝐫𝐚𝐭𝐢𝐨𝐧 𝐄𝐟𝐟𝐢𝐜𝐢𝐞𝐧𝐜𝐲: Superior performance and reduced pressure drop. High Flow Rates: Optimized for efficient processing. For Know About Carbon Lenticular Filter https://buff.ly/40xOHF2 For more information and to explore how TRINITY can support your filtration needs, please contact us at [email protected] #TRINITY #lenticularfilters #biopharma #biotech #filtration #pharmaceuticalgrade #qualitycontrol #processoptimization

𝐓𝐑𝐈𝐍𝐈𝐓𝐘 𝐂𝐡𝐚𝐫𝐠𝐞 𝐏𝐥𝐮𝐬 𝐋𝐞𝐧𝐭𝐢𝐜𝐮𝐥𝐚𝐫 𝐅𝐢𝐥𝐭𝐞𝐫𝐬: 𝐀 𝐏𝐨𝐰𝐞𝐫𝐟𝐮𝐥 𝐒𝐨𝐥𝐮𝐭𝐢𝐨𝐧 𝐟𝐨𝐫 𝐁𝐢𝐨𝐩𝐡𝐚𝐫𝐦𝐚𝐜𝐞𝐮𝐭𝐢𝐜𝐚𝐥 𝐚𝐧𝐝 𝐁𝐢𝐨𝐭𝐞𝐜𝐡 𝐀𝐩𝐩𝐥𝐢𝐜𝐚𝐭𝐢𝐨𝐧𝐬 TRINITY Charge Plus Lenticular Filters are cutting-edge filtration solutions designed to meet the stringent requirements of the biopharmaceutical and biotech industries. These positively charged cellulose depth filters are formulated to deliver exceptional performance and reliability. 𝐊𝐞𝐲 𝐅𝐞𝐚𝐭𝐮𝐫𝐞𝐬 𝐚𝐧𝐝 𝐁𝐞𝐧𝐞𝐟𝐢𝐭𝐬: 𝐌𝐞𝐜𝐡𝐚𝐧𝐢𝐜𝐚𝐥 𝐚𝐧𝐝 𝐄𝐥𝐞𝐜𝐭𝐫𝐨-𝐊𝐢𝐧𝐞𝐭𝐢𝐜 𝐀𝐝𝐬𝐨𝐫𝐩𝐭𝐢𝐯𝐞 𝐂𝐚𝐩𝐭𝐮𝐫𝐞: Effectively removes particulate matter, microorganisms, colloids, and pyrogens from critical process streams. 𝐄𝐧𝐡𝐚𝐧𝐜𝐞𝐝 𝐅𝐢𝐥𝐭𝐫𝐚𝐭𝐢𝐨𝐧 𝐄𝐟𝐟𝐢𝐜𝐢𝐞𝐧𝐜𝐲: Superior performance and reduced pressure drop. High Flow Rates: Optimized for efficient processing. For Know About Carbon Lenticular Filter https://buff.ly/40xOHF2 For more information and to explore how TRINITY can support your filtration needs, please contact us at [email protected] #TRINITY #lenticularfilters #biopharma #biotech #filtration #pharmaceuticalgrade #qualitycontrol #processoptimization

In this #article with Manufacturing Chemist, Dr. Andy Lewis, Chief Scientific Officer, examines trends driving the adoption of oral #peptide products and discusses strategies to overcome the #development challenges associated with effective delivery. Read the full article here: https://bit.ly/4dq7Ah4

Investigation of Fouling in Perfusion Cell Culture Processes Using Alternating Tangential Flow Filtration Microfiltration membranes are increasingly used to retain the cells inside bioreactors while continuous harvest of the desired biopharmaceutical occurs in perfusion processes. One method of microfiltration is Alternating Tangential Flow (ATF) filtration, which involves moving the cultivation broth tangentially back and forth across the membrane, which results in a backwash effect that reduces fouling. In this study, fouling was investigated with asymmetric polysulfone hollow fibers operated in ATF mode attached to a bioreactor producing a recombinant protein in Chinese Hamster Ovary (CHO) cells. Fouling was assessed through different approaches, including determination of critical flux using an improved flux-step method. https://lnkd.in/grZSYpMD #aspenalert #biotech #bioprocess

Can disc-stack centrifuges double as homogenizers? 🤔 Last week a bioprocess engineer questioned us if we employed disc stack centrifugation technology in our cRUSH system. It's an intriguing concept, especially considering the high cell concentration achieved at the periphery of the discs. While developing our single-use continuous homogenizer, cRUSH, we initially explored this approach. And yes, stacks of discs indeed partially homogenized cells (up to 70%) with surprising ease. However, despite rigorous testing, we couldn't achieve 100% homogenization in a single pass. While multiple passes might yield different results, our findings suggest that while promising, disc-stack centrifuges alone might not be the complete solution for efficient cell lysis. On the other hand, our single-use cRUSH systems using a completely different technology achieved 100% lysis of cells in a single pass. www.athembio.com #biotechnology #cellculture #homogenization #discstackcentrifuge #research #development #cRUSH #singleuse #AthemBio

Tired of pulsation and variation in the flow of your biopharma media? With up to 90% reduction in peak-to-peak flow pulsation, our Pulsation Dampener from Malema, a PSG brand, has a pressure rating of 90 psi, or 6 bar. Learn more about our solutions from Malema, a PSG brand https://hp-ne.com/malema/ #innovation #biopharma #biotech #bioprocessing