We've come a long way from making 10mers by hand in grad school: Our gene synthesis service at Ansa Biotechnologies, Inc. can now build DNA constructs up to 5 kb more reliably than anyone else on the market, including those with complex features.
Create an account at www.ansabio.com and get your 5 kb synthetic genes on time, regardless of sequence complexity.
I am happy to share that our engineering and characterization of the Neq2X7 DNA polymerase for PCR applications is now published in BMC Biotechnology. Thanks Cristina Hernández Rollán and Morten Nørholm for the collaboration on this project!
An earlier version of this manuscript was available on bioRxiv for a while already, but we expanded the project quite a bit for this new version, adding an experiment to determine the error rate of Neq2X7 and characterizing its tolerance to a number of common inhibitory substances.
https://lnkd.in/dDY8dFUZ
The expression plasmids for Neq2X7 and PfuX7 are available via addgene.com if you want to try it out for yourself!
https://lnkd.in/dfJJwGV3
💡 Why did we do this and want to share?
🔬 Have you ever really thought about which polymerase you are using for both DNA assembly and colony/diagnostic PCR? Different DNA polymerases can have wildly different properties.
👩🔬 Have you ever really looked at your PCR program and adjusted the settings to the recommendations for your polymerase or based on your experience? Or are you still just using the same program that you copy-pasted from the postdoc who trained you?
🚀 It is valuable to explore alternatives for your materials and workflows, it could potentially save your lab a lot of money, time and PCR-related headaches.
💸 Molecular biology and synthetic biology labs spend incredible amounts of money on DNA polymerases, we hope that Neq2X7 can be a useful addition to the toolbox combining high performance with an easy DIY-production protocol for labs with low financial resources as well as in teaching labs.
Apprentice Trainee (Team Incharge & Operational Reporting) @JFL | Research Intern at IIT (Banaras Hindu University) | Intrested towards Waste to Energy Solutions | Final year Biotechnology Student | @RRIMT..
Agarose Gel Electrophoresis in Molecular Research
As a cornerstone technique in molecular biology, agarose gel electrophoresis has empowered researchers to visualize, analyze, and separate nucleic acids with incredible precision. Whether you’re studying DNA, RNA, or even protein fragments, this method remains indispensable in labs around the world.
Here’s why agarose gel electrophoresis is so crucial:
🔬 Separation of Molecules: Agarose gel electrophoresis separates nucleic acid fragments based on size, making it invaluable for tasks like gene analysis, PCR validation, and DNA sequencing.
⚡ Visualization: By applying an electric field, researchers can view the movement of DNA or RNA under UV light, allowing quick and clear interpretation of results.
🔍 Applications:
Genotyping: Identifying genetic variations with ease.
PCR Analysis: Verifying PCR products and ensuring they match expected sizes.
Molecular Cloning: Confirming insertions or deletions in recombinant DNA projects.
🎯 Precision & Efficiency: This simple yet powerful method provides results that are not only reproducible but also fast, giving scientists the ability to advance their experiments with confidence.
At its core, agarose gel electrophoresis is more than just a lab technique—it’s a gateway to innovation, enabling breakthroughs in genetic research, diagnostics, and biotechnology.
#MolecularBiology#AgaroseGelElectrophoresis#DNAAnalysis#GeneticResearch#LabTech#Biotech
Professor and Translational Vision Scientist, Eye Research Institute, Past President OU-AAUP, Inventor, Oakland University. With past lives as a DJ 1450 CHCL and SAR navigator pilot.
Looking east front the Control of Gene Expression lab in the Eye Research Institute. We have some red leaves, a little behind schedule in our warming climate. This is the view from the lab where we invented a biotechnology production process for Noregen, a protein therapeutic for a compromised retinal vasculature, with NEI STTR phase1 support. Noregen development continues with NEI SBIR phase2 support and our small scale bacterial production process has been transfered to big scale production by Caeregen Therapeutics*.
You can only get the knowhow to do these things from a broad training and experience in many areas of biochemistry, molecular biology, and physiology. Over many years at more than one institution. Using knowledge from all over the world.
That mostly only happens in Universities like ours. So support America's universities and students. They provide us with a future.
Oakland University
(*I am not an employee nor investor. I provided my IP for zero compensation to Noregen's developers.)
We are excited to announce the next speaker for ESACT-UK 2025
🌟Introducing the next speaker in our series: Mario Pereira🌟
Mario is the Director of Technologies and Business Development at ATUM, bringing over 15 years of combined academic and industrial expertise in recombinant protein expression. He earned his PhD in Biotechnology from the University of Manchester, where he investigated the transcriptional mechanisms that regulate recombinant promoters in CHO cells. Following his doctoral studies, Mario pursued his passion for biotherapeutic manufacturing at FUJIFILM Diosynth Biotechnologies and Horizon Discovery, where he had different scientific and commercial-related roles. Passionate about technology development, Mario is driven by a desire to translate scientific discoveries into impactful technologies.
❗Title: From Landing Pads to Transposases to CRISPR: What Have Gene Editing Tools Given to Biomanufacturing, and Where Can They Take Us?
🌟Session: 2 - Engineering biology in bioprocessing
📅 Date: 8th January 2025
📌 Stay connected for the latest updates! Follow our page to catch every episode of "Meet the Speakers." 🎤
#MeetTheSpeakers#Innovation#Research#Bioengineering#Academia#ThoughtLeadership#LinkedInSeries
Scientists are on their way to designing and making tailored proteins. By combining AI’s design talents and gene editing’s engineering abilities, scientists can modify bacteria to act as mini-factories producing new proteins that can reduce greenhouse gases, digest plastics, or act as species-specific pesticides https://bit.ly/4bEsd7e
🎓 Thesis Completed!
I’m excited to share that I have successfully completed my Master’s Thesis in Bioengineering: Cell and Gene Biotechnology, with a major in Bioinformatics, at Ghent University!🔬
During this project, hosted by IQS Barcelona, I focused on the metabolic engineering of Saccharomyces cerevisiae to boost ceramide production, which has significant applications in the pharmaceutical and health industries, as well as broader industrial bioprocessing. 🧬💊
A big thank you to my advisors and colleagues at UGent and IQS for their support throughout this journey. I’m eager to leverage my skills in both computational and metabolic engineering to tackle future challenges in the pharma and industrial biotech sectors. 🚀💡
#MetabolicEngineering#Pharma#Ceramides#Bioinformatics#SaccharomycesCerevisiae#IndustrialBiotech#ThesisCompleted#UGent#IQS#HealthIndustry#Research