“Rochelle and I worked together at BRT, where she was a Study Director and I was the QA Manager. She was the key contact for many immunotoxicity studies and had to liaison with Sponsors, QA, and laboratory personnel. Her reports were skillfully written and she was always willing to explain scientific nuances to someone less experienced. She is talented, supportive of colleagues and clients, and has excellent time management skills. She fosters a collaborative environment and working with her was always a pleasure. ”
Rochelle Mills
Managing Partner & Senior Medical Writer at SRM Management Science Company
Richmond, Virginia, United States
731 followers
500+ connections
About
PhD scientist with expertise in the fields of medical writing, regulatory submissions, clinical and nonclinical summaries, oncology, immunotherapy, infectious disease (antibacterial, antifungal, and antiviral), vaccines and adjuvants, pain management, cardiology, and toxicology. Excited to contribute to well-designed studies and communicate critical data and results in written or oral form. Invigorated by team work and collaboration.
Interested in networking and collaboration to advance research and progress personally as a scientific/regulatory writer. Also interested in advancing the careers of women in science through mentoring and education.
Specialties: Regulatory & Scientific Writing, Project Management, GCP, GLP & Nonclinical, Oncology, Immunology/Immunotherapy, Toxicology, Vaccines, Infectious Disease.
Activity
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I am really proud of the GIVAX team that is pushing a new vaccine concept to fight GI viral infections. An oral vaccine for infant protection…
I am really proud of the GIVAX team that is pushing a new vaccine concept to fight GI viral infections. An oral vaccine for infant protection…
Liked by Rochelle Mills
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First LinkedIn post ever! I did a thing that I think is cool. Check it out! https://lnkd.in/gYRb6wVj
First LinkedIn post ever! I did a thing that I think is cool. Check it out! https://lnkd.in/gYRb6wVj
Liked by Rochelle Mills
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Today, I was promoted to Associate Professor of Psychiatry & Behavioral Sciences and Pediatrics (with tenure) at the The George Washington University…
Today, I was promoted to Associate Professor of Psychiatry & Behavioral Sciences and Pediatrics (with tenure) at the The George Washington University…
Liked by Rochelle Mills
Experience
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Managing Partner & Senior Medical Writer
SRM Management Science Company
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Graduate Women in Science, Research Triangle Chapter
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President
Graduate Women in Science, Research Triangle Chapter
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Secretary
Graduate Women in Science, Research Triangle Chapter
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Graduate Research Assistant (NIH Ruth L. Kirschstein National Research Service Award Trainee)
North Carolina State University
Education
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North Carolina State University
PhD Immunology, minor biotechnology
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Activities and Societies: Lectured on quantitative PCR for graduate students and in an immunology laboratory for DVM students. Manuscript co-reviewer for Blood. Mentored two DVM students in the Merck-Merial Veterinary Scholars Program. Recruited and mentored a high school student during a semester-long research project. NCSU College of Veterinary Medicine Graduate Student Association Secretary. NC DNA Day Mentor. Organized science outreach activities for Dr. Gregg Dean’s laboratory.
Ruth L. Kirschstein National Research Service Award Trainee in the NCSU Molecular Biotechnology Training Program, 2006-2008.
Keystone Symposia HIV Pathogenesis Student Travel Scholarship, 2008. -
University of Alabama
BS Microbiology and Immunology
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Activities and Societies: Howard Hughes Medical Research Intern in 2003 and 2004. Tutored for the University of Alabama Rural Science Scholars Program. Managed an undergraduate summer intern.
Volunteer Experience
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Secretary
Graduate Student Association, College of Veterinary Medicine (NCSU)
- 1 year
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Chapter Adviser
Graduate Women in Science, Rho Tau Chapter
- 2 years 1 month
Science and Technology
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Chair, Chapter Establishment Committee
Graduate Women in Science, National
- 2 years
Science and Technology
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Member, Programs Committee
Immunotoxicology Specialty Section, Society of Toxicology
- 1 year 3 months
Science and Technology
Publications
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Regulatory T cell depletion prior to acute feline immunodeficiency virus infection does not alter disease pathogenesis.
PLoS ONE
Feline immunodeficiency virus (FIV) infection in cats follows a disease course similar to HIV-1, including a short acute phase characterized by high viremia, and a prolonged asymptomatic phase characterized by low viremia and generalized immune dysfunction. CD4+CD25hiFoxP3+ immunosuppressive regulatory T (Treg) cells have been implicated as a possible cause of immune dysfunction during FIV and HIV-1 infection, as they are capable of modulating virus-specific and inflammatory immune responses…
Feline immunodeficiency virus (FIV) infection in cats follows a disease course similar to HIV-1, including a short acute phase characterized by high viremia, and a prolonged asymptomatic phase characterized by low viremia and generalized immune dysfunction. CD4+CD25hiFoxP3+ immunosuppressive regulatory T (Treg) cells have been implicated as a possible cause of immune dysfunction during FIV and HIV-1 infection, as they are capable of modulating virus-specific and inflammatory immune responses. Additionally, the immunosuppressive capacity of feline Treg cells has been shown to be increased during FIV infection. We have previously shown that transient in vivo Treg cell depletion during asymptomatic FIV infection reveals FIV-specific immune responses suppressed by Treg cells. In this study, we sought to determine the immunological influence of Treg cells during acute FIV infection. We asked whether Treg cell depletion prior to infection with the highly pathogenic molecular clone FIV-C36 in cats could alter FIV pathogenesis. We report here that partial Treg cell depletion prior to FIV infection does not significantly change provirus, viremia, or CD4+ T cell levels in blood and lymphoid tissues during the acute phase of disease. The effects of anti-CD25 mAb treatment are truncated in cats acutely infected with FIV-C36 as compared to chronically infected cats or FIV-naïve cats, as Treg cell levels were heightened in all treatment groups included in the study within two weeks post-FIV infection. Our findings suggest that the influence of Treg cell suppression during FIV pathogenesis is most prominent after Treg cells are activated in the environment of established FIV infection.
Other authorsSee publication -
In vivo depletion of CD4+CD25hi regulatory T cells is associated with improved antiviral responses in cats chronically infected with feline immunodeficiency virus.
Virology
Regulatory T (Treg) cells are activated and suppress immune responses during infection, and are characterized as CD4(+)CD25(hi)FOXP3(+). Ex vivo studies demonstrate that Treg cells potentially suppress anti-HIV-1 T cell responses. Lentivirus-induced CD4(+)CD25(hi) Treg cells were first described in feline immunodeficiency virus (FIV)-infected cats. In the present study we demonstrate that anti-feline CD25 monoclonal antibody (mAb) therapy depletes Treg cells in FIV-infected cats for 4 weeks and…
Regulatory T (Treg) cells are activated and suppress immune responses during infection, and are characterized as CD4(+)CD25(hi)FOXP3(+). Ex vivo studies demonstrate that Treg cells potentially suppress anti-HIV-1 T cell responses. Lentivirus-induced CD4(+)CD25(hi) Treg cells were first described in feline immunodeficiency virus (FIV)-infected cats. In the present study we demonstrate that anti-feline CD25 monoclonal antibody (mAb) therapy depletes Treg cells in FIV-infected cats for 4 weeks and does not exacerbate viral replication or proinflammatory cytokine production. Significant FIV-specific immune responses are revealed in Treg cell-depleted cats. These anti-FIV effector cells exist prior to Treg cell depletion and are not expanded while Treg cells are depleted. Importantly, cats receiving the Treg cell-depleting mAb are able to produce a robust humoral response to new antigen. We propose that short-term in vivo Treg cell depletion during chronic HIV-1 infection could provide a window of opportunity for therapeutic vaccination in individuals with controlled viral replication.
Other authorsSee publication -
In vivo depletion of CD4+CD25+ regulatory T cells in cats.
Journal of Immunological Methods
To establish a characterized model of regulatory T cell (Treg) depletion in the cat we assessed the kinetics of depletion and rebound in peripheral and central lymphoid compartments after treatment with anti-CD25 antibody as determined by cell surface markers and FOXP3 mRNA expression. An 82% decrease in circulating CD4+CD25+ Tregs was observed by day 11 after treatment. CD4+CD25+ cells were also reduced in the thymus (69%), secondary lymphoid tissues (66%), and gut (67%). Although CD4+CD25+…
To establish a characterized model of regulatory T cell (Treg) depletion in the cat we assessed the kinetics of depletion and rebound in peripheral and central lymphoid compartments after treatment with anti-CD25 antibody as determined by cell surface markers and FOXP3 mRNA expression. An 82% decrease in circulating CD4+CD25+ Tregs was observed by day 11 after treatment. CD4+CD25+ cells were also reduced in the thymus (69%), secondary lymphoid tissues (66%), and gut (67%). Although CD4+CD25+ cells rebound by day 35 post-treatment, FOXP3 levels remain depressed suggesting anti-CD25 antibody treatment has a sustainable diminutive effect on the Treg population. To determine whether CD25+ Treg depletion strategies also deplete activated CD25+ effector cells, cats were immunized with feline immunodeficiency virus (FIV) p24-GST recombinant protein, allowing them to develop a measurable memory response, prior to depletion with anti-CD25 antibody. Anti-FIV p24-GST effector cell activity in peripheral blood after depletion was sustained as determined by antigen-specific T cell proliferation and humoral responses against FIV p24-GST with an ELISA for antigen-specific feline IgG. Furthermore, development of an anti-mouse response in Treg-depleted cats was similar to control levels indicating the retained capacity to respond to a novel antigen. We conclude that despite alterations in CD25+ cell levels during depletion, the feline immune system remains functional. We demonstrate here a model for the study of disease pathogenesis in the context of reduced numbers of immunosuppressive CD4+CD25+ Tregs throughout the feline immune system.
Other authorsSee publication -
Plasmid-specified FemABX-like immunity factor in Staphylococcus sciuri DD 4747.
FEMS Microbiology Letters
A plasmid from Staphylococcus sciuri DD 4747 had three open reading frames: a replication gene, an N-acetylmuramyl-l-alanine amidase-like gene, and a gene similar to the lysostaphin endopeptidase resistance gene (epr/lif). The epr-like gene was introduced into S. aureus RN4220; the recombinant strain was more resistant to lysostaphin endopeptidase and its cell wall peptidoglycan contained more serines and fewer glycines than the parental strain with the shuttle vector alone. Based on both its…
A plasmid from Staphylococcus sciuri DD 4747 had three open reading frames: a replication gene, an N-acetylmuramyl-l-alanine amidase-like gene, and a gene similar to the lysostaphin endopeptidase resistance gene (epr/lif). The epr-like gene was introduced into S. aureus RN4220; the recombinant strain was more resistant to lysostaphin endopeptidase and its cell wall peptidoglycan contained more serines and fewer glycines than the parental strain with the shuttle vector alone. Based on both its function and its similarity to femAB, this gene is a member of the femABX-like immunity gene family. Furthermore, this is the first example of a femABX-like immunity gene that is not linked to the gene for the bacteriolytic enzyme against which it specifies immunity.
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The streptococcolytic enzyme zoocin A is a penicillin-binding protein
FEMS Microbiology Letters
Zoocin A is a streptococcolytic enzyme produced by Streptococcus equi subsp. zooepidemicus 4881 that has an unknown site of action on the peptidoglycans of susceptible organisms. Analysis of a mutant strain in which the genes for zoocin A and resistance to zoocin A were inactivated revealed that this strain was more susceptible to β-lactam antibiotics than the parental organism. Purified zoocin A had weak β-lactamase activity, bound radioactive penicillin covalently, and its streptococcolytic…
Zoocin A is a streptococcolytic enzyme produced by Streptococcus equi subsp. zooepidemicus 4881 that has an unknown site of action on the peptidoglycans of susceptible organisms. Analysis of a mutant strain in which the genes for zoocin A and resistance to zoocin A were inactivated revealed that this strain was more susceptible to β-lactam antibiotics than the parental organism. Purified zoocin A had weak β-lactamase activity, bound radioactive penicillin covalently, and its streptococcolytic activity was inhibited by penicillin. Thus, zoocin A is a penicillin-binding protein and presumably is a d-alanyl endopeptidase.
Other authors
Courses
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American College of Toxicology: Toxicology for Pharmaceutical and Regulatory Scientists (April 2016)
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Projects
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Presentation: Managing Your Online Professional Network
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Presented hour-long seminars about maximizing the utility of LinkedIn and networking to six groups from 2012-2015.
• RTI International, RTP, NC, June 2012
• The Church of Jesus Christ of Latter-day Saints, Durham, NC, September 2012
• University of Alabama at Birmingham Graduate Career Awareness and Trends (UAB GCAT), Birmingham, AL, September 2012
• University of North Carolina-Chapel Hill Career Symposium for Graduate Students and Post-doctoral Scholars, February 2013 (with…Presented hour-long seminars about maximizing the utility of LinkedIn and networking to six groups from 2012-2015.
• RTI International, RTP, NC, June 2012
• The Church of Jesus Christ of Latter-day Saints, Durham, NC, September 2012
• University of Alabama at Birmingham Graduate Career Awareness and Trends (UAB GCAT), Birmingham, AL, September 2012
• University of North Carolina-Chapel Hill Career Symposium for Graduate Students and Post-doctoral Scholars, February 2013 (with Katherine Nobles)
• The Church of Jesus Christ of Latter-day Saints, Raleigh, NC, February 2014
• Duke University Women in Science & Engineering, Durham, NC, January 2015
Honors & Awards
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American Society for Virology Postdoctoral Fellow Travel Award
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Keystone Symposia HIV Pathogenesis Student Travel Scholarship
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Ruth L. Kirschstein National Research Service Award Trainee
NIH/NCSU Molecular Biotechnology Training Program
Participated 2006-2008.
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Howard Hughes Medical Research Intern
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Howard Hughes Medical Research Intern
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Organizations
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American Medical Writers Association
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- Present -
Society of Toxicology
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- Present -
Graduate Women in Science
President, Secretary, Chapter Adviser, Chapter Establishment Committee Chair
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Women in Bio
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American Society for Virology
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American Association of Immunologists
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American Society for Microbiology
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Recommendations received
7 people have recommended Rochelle
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It's not about the people, it's about the RIGHT people! #TheinSeptionDifference #PeoplePurposePerformance
It's not about the people, it's about the RIGHT people! #TheinSeptionDifference #PeoplePurposePerformance
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