Cryo-Electron Microscopy
Dr. Ran ZalkHead
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The Electron Microscopy unit provides the expertise and resources for solving macromolecular structures. For structural determination, a high-end transmission electron microscope (FEI Tecnai F30 Polara), a direct detection camera (Gatan K2 Summit with Quantum GIF energy filter), and the computational support necessary for single particle analysis are available. Higher order structure determination is possible through tomographic reconstruction using the same setup. The unit also offers grid sample preparation expertise and equipment (Leica EM GP).
The equipment and services of the Electron Microscopy Unit are available for use by NIBN members and BGU faculty, as well as researchers at other universities and in industry.
- Email: [email protected]
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Main Services
Expertise and resources for solving macromolecular structures
Grid sample preparation expertise and equipment (Leica EM GP)
Single particle analysis for structural determination (Gatan K2 Summit with Quantum GIF energy filter)
Publications
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Sporny M., Guez-Haddad J., Khazma T., Yaron A., Dessau M., Shkolnisky Y., Mim C., Isupov M.N., Zalk R., Hons M., Opatowsky Y. (2020)
Structural basis for SARM1 inhibition and activation under energetic stress
ELife. 9:e62021. -
Davidov G., Abelya G., Zalk R., Izbicki B., Shaibi S., Spektor L., Shagidov D., Meyron-Holtz E.G., Zarivach R., Frank G.A. (2020)
Folding of an Intrinsically Disordered Iron-Binding Peptide in Response to Sedimentation Revealed by Cryo-EM
J. Am. Chem. Soc. 142(46):19551-19557. -
Dridi H., Kushnir A., Zalk R., Yuan Q,., Melville Z., Marks A.R. (2020)
Intracellular calcium leak in heart failure and atrial fibrillation: a unifying mechanism and therapeutic target
Nat. Rev. Cardiol. 17(11):732-747. -
Drabkin M., Yogev Y., Zeller L., Zarivach R., Zalk R., Halperin D., Wormser O., Gurevich E., Landau D., Kadir R., Perez Y., Birk O.S. (2019)
Hyperuricemia and gout caused by missense mutation in d-lactate dehydrogenase
J. Clin. Invest. 129(12):5163-5168. -
Kopatz I., Zalk R., Levi-Kalisman Y., Zlotkin-Rivkin E., Frank G.A., Kler S. (2019)
Packaging of DNA origami in viral capsids
NaNoscale. 11(21):10160-10166.